Restriction map for
pF1KB9876
(Linear) (Six-base) MAPSORT of: KIBB9876.seq Check: 3179 from: 1 to: 1626
KIBB9876.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1012 1626
Size: 1012 614
Acc65I G'GTAC_C
Cuts at: 0 567 1626
Size: 567 1059
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 494 575 1336 1472 1626
Size: 152 342 81 761 136 154
Fragments arranged by size:
761 342 154 152 136 81
AflIII A'CryG_T
Cuts at: 0 254 1626
Size: 254 1372
AhdI GACnn_n'nnGTC
Cuts at: 0 335 437 1545 1626
Size: 335 102 1108 81
Fragments arranged by size:
1108 335 102 81
ApaI G_GGCC'C
Cuts at: 0 1085 1626
Size: 1085 541
ApoI r'AATT_y
Cuts at: 0 45 159 210 961 1263 1626
Size: 45 114 51 751 302 363
Fragments arranged by size:
751 363 302 114 51 45
BanI G'GyrC_C
Cuts at: 0 567 1626
Size: 567 1059
BanII G_rGCy'C
Cuts at: 0 385 1085 1626
Size: 385 700 541
Fragments arranged by size:
700 541 385
BbsI GAAGACnn'nnnn_
Cuts at: 0 347 593 883 998 1541 1579 1626
Size: 347 246 290 115 543 38 47
Fragments arranged by size:
543 347 290 246 115 47 38
BbvCI CC'TCA_GC
Cuts at: 0 901 1626
Size: 901 725
BglII A'GATC_T
Cuts at: 0 90 1626
Size: 90 1536
Bme1580I G_kGCm'C
Cuts at: 0 1085 1626
Size: 1085 541
BmrI ACTGGGnnnn_n'
Cuts at: 0 398 1626
Size: 398 1228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1626
Size: 482 1144
Bpu10I CC'TnA_GC
Cuts at: 0 901 1626
Size: 901 725
BsaI GGTCTCn'nnnn_
Cuts at: 0 462 1626
Size: 462 1164
BsaAI yAC'GTr
Cuts at: 0 255 1626
Size: 255 1371
BsaBI GATnn'nnATC
Cuts at: 0 83 89 1626
Size: 83 6 1537
Fragments arranged by size:
1537 83 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 440 470 1626
Size: 440 30 1156
Fragments arranged by size:
1156 440 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 479 1626
Size: 85 394 1147
Fragments arranged by size:
1147 394 85
BseYI C'CCAG_C
Cuts at: 0 986 1626
Size: 986 640
BsiEI CG_ry'CG
Cuts at: 0 328 1626
Size: 328 1298
BsiHKAI G_wGCw'C
Cuts at: 0 385 1406 1626
Size: 385 1021 220
Fragments arranged by size:
1021 385 220
Bsp1286I G_dGCh'C
Cuts at: 0 385 1085 1406 1626
Size: 385 700 321 220
Fragments arranged by size:
700 385 321 220
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1012 1626
Size: 1012 614
BsrDI GCAATG_nn'
Cuts at: 0 758 1626
Size: 758 868
BsrFI r'CCGG_y
Cuts at: 0 303 1019 1367 1626
Size: 303 716 348 259
Fragments arranged by size:
716 348 303 259
BsrGI T'GTAC_A
Cuts at: 0 117 1065 1626
Size: 117 948 561
Fragments arranged by size:
948 561 117
BssSI C'ACGA_G
Cuts at: 0 1460 1626
Size: 1460 166
BstYI r'GATC_y
Cuts at: 0 84 90 635 720 1626
Size: 84 6 545 85 906
Fragments arranged by size:
906 545 85 84 6
BtgI C'CryG_G
Cuts at: 0 1076 1626
Size: 1076 550
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1056 1626
Size: 1056 570
BtsI GCAGTG_nn'
Cuts at: 0 1011 1626
Size: 1011 615
DraI TTT'AAA
Cuts at: 0 894 1431 1626
Size: 894 537 195
Fragments arranged by size:
894 537 195
EcoICRI GAG'CTC
Cuts at: 0 383 1626
Size: 383 1243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 482 494 575 1336 1472 1626
Size: 152 330 12 81 761 136 154
Fragments arranged by size:
761 330 154 152 136 81 12
EcoO109I rG'GnC_Cy
Cuts at: 0 1081 1082 1131 1347 1626
Size: 1081 1 49 216 279
Fragments arranged by size:
1081 279 216 49 1
EcoRV GAT'ATC
Cuts at: 0 9 1626
Size: 9 1617
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 321 342 353 423 440 455 472
Size: 310 11 21 11 70 17 15 17
Cuts at: 472 780 812 1196 1228 1531 1563 1626
Size: 308 32 384 32 303 32 63
Fragments arranged by size:
384 310 308 303 70 63 32 32
32 21 17 17 15 11 11
KpnI G_GTAC'C
Cuts at: 0 571 1626
Size: 571 1055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 1306 1626
Size: 426 880 320
Fragments arranged by size:
880 426 320
MslI CAynn'nnrTG
Cuts at: 0 125 1075 1626
Size: 125 950 551
Fragments arranged by size:
950 551 125
MspA1I CmG'CkG
Cuts at: 0 905 986 1626
Size: 905 81 640
Fragments arranged by size:
905 640 81
NaeI GCC'GGC
Cuts at: 0 1369 1626
Size: 1369 257
NcoI C'CATG_G
Cuts at: 0 1076 1626
Size: 1076 550
NgoMIV G'CCGG_C
Cuts at: 0 1367 1626
Size: 1367 259
NspI r_CATG'y
Cuts at: 0 118 1626
Size: 118 1508
PasI CC'CwG_GG
Cuts at: 0 427 562 1626
Size: 427 135 1064
Fragments arranged by size:
1064 427 135
PmlI CAC'GTG
Cuts at: 0 255 1626
Size: 255 1371
PpuMI rG'GwC_Cy
Cuts at: 0 1131 1347 1626
Size: 1131 216 279
Fragments arranged by size:
1131 279 216
PsiI TTA'TAA
Cuts at: 0 374 1626
Size: 374 1252
PspOMI G'GGCC_C
Cuts at: 0 1081 1626
Size: 1081 545
PvuI CG_AT'CG
Cuts at: 0 328 1626
Size: 328 1298
PvuII CAG'CTG
Cuts at: 0 905 986 1626
Size: 905 81 640
Fragments arranged by size:
905 640 81
SacI G_AGCT'C
Cuts at: 0 385 1626
Size: 385 1241
SexAI A'CCwGG_T
Cuts at: 0 366 1626
Size: 366 1260
SfcI C'TryA_G
Cuts at: 0 1045 1372 1626
Size: 1045 327 254
Fragments arranged by size:
1045 327 254
SspI AAT'ATT
Cuts at: 0 927 1626
Size: 927 699
StyI C'CwwG_G
Cuts at: 0 679 1076 1412 1626
Size: 679 397 336 214
Fragments arranged by size:
679 397 336 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1200 1626
Size: 1200 426
TatI w'GTAC_w
Cuts at: 0 117 1065 1626
Size: 117 948 561
Fragments arranged by size:
948 561 117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 661 1162 1626
Size: 661 501 464
Fragments arranged by size:
661 501 464
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI ApaI ApoI BanI
BanII BbsI BbvCI BglII Bme1580I BmrI BpmI Bpu10I
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BsrGI BssSI BstYI BtgI
BtgZI BtsI DraI EcoICRI Eco57MI EcoO109I EcoRV Hin4I
KpnI MmeI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PmlI PpuMI PsiI PspOMI PvuI PvuII SacI
SexAI SfcI SspI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI BpuEI BsaHI BsaWI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoNI EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MscI NarI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PfoI PmeI PpiI PshAI
PspXI PsrI PstI RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI