Restriction map for
pF1KB9878
(Linear) (Six-base) MAPSORT of: KIBB9878.seq Check: 5047 from: 1 to: 1725
KIBB9878.orf
With 182 enzymes: SgfI *
January 12, 2011 16:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 188 1672 1725
Size: 188 1484 53
Fragments arranged by size:
1484 188 53
AatII G_ACGT'C
Cuts at: 0 808 1725
Size: 808 917
AccI GT'mk_AC
Cuts at: 0 1247 1725
Size: 1247 478
Acc65I G'GTAC_C
Cuts at: 0 1030 1725
Size: 1030 695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1192 1259 1514 1725
Size: 376 816 67 255 211
Fragments arranged by size:
816 376 255 211 67
AfeI AGC'GCT
Cuts at: 0 1620 1725
Size: 1620 105
AleI CACnn'nnGTG
Cuts at: 0 103 1725
Size: 103 1622
ApaI G_GGCC'C
Cuts at: 0 216 1725
Size: 216 1509
AvaI C'yCGr_G
Cuts at: 0 169 376 679 698 1159 1725
Size: 169 207 303 19 461 566
Fragments arranged by size:
566 461 303 207 169 19
BanI G'GyrC_C
Cuts at: 0 91 165 1030 1725
Size: 91 74 865 695
Fragments arranged by size:
865 695 91 74
BanII G_rGCy'C
Cuts at: 0 128 216 688 1544 1725
Size: 128 88 472 856 181
Fragments arranged by size:
856 472 181 128 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 364 1104 1725
Size: 364 740 621
Fragments arranged by size:
740 621 364
BbvCI CC'TCA_GC
Cuts at: 0 13 42 68 1725
Size: 13 29 26 1657
Fragments arranged by size:
1657 29 26 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 693 727 1725
Size: 693 34 998
Fragments arranged by size:
998 693 34
Bme1580I G_kGCm'C
Cuts at: 0 96 170 216 527 1725
Size: 96 74 46 311 1198
Fragments arranged by size:
1198 311 96 74 46
BmgBI CAC'GTC
Cuts at: 0 842 1725
Size: 842 883
BmrI ACTGGGnnnn_n'
Cuts at: 0 1348 1485 1725
Size: 1348 137 240
Fragments arranged by size:
1348 240 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 778 849 1221 1725
Size: 205 573 71 372 504
Fragments arranged by size:
573 504 372 205 71
Bpu10I CC'TnA_GC
Cuts at: 0 13 42 68 1725
Size: 13 29 26 1657
Fragments arranged by size:
1657 29 26 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 1725
Size: 206 1519
BsaAI yAC'GTr
Cuts at: 0 1503 1725
Size: 1503 222
BsaHI Gr'CG_yC
Cuts at: 0 470 805 1177 1725
Size: 470 335 372 548
Fragments arranged by size:
548 470 372 335
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 523 553 886 916 1725
Size: 523 30 333 30 809
Fragments arranged by size:
809 523 333 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 56 559 586 631 634 1515 1552 1725
Size: 56 503 27 45 3 881 37 173
Fragments arranged by size:
881 503 173 56 45 37 27 3
BseYI C'CCAG_C
Cuts at: 0 75 387 460 564 1191 1725
Size: 75 312 73 104 627 534
Fragments arranged by size:
627 534 312 104 75 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1650 1725
Size: 1650 75
BsiEI CG_ry'CG
Cuts at: 0 252 284 450 514 1725
Size: 252 32 166 64 1211
Fragments arranged by size:
1211 252 166 64 32
BsiHKAI G_wGCw'C
Cuts at: 0 688 1544 1725
Size: 688 856 181
Fragments arranged by size:
856 688 181
BsmBI CGTCTCn'nnnn_
Cuts at: 0 340 373 754 889 1725
Size: 340 33 381 135 836
Fragments arranged by size:
836 381 340 135 33
Bsp1286I G_dGCh'C
Cuts at: 0 96 128 170 216 527 688 1544 1725
Size: 96 32 42 46 311 161 856 181
Fragments arranged by size:
856 311 181 161 96 46 42 32
BspHI T'CATG_A
Cuts at: 0 980 1725
Size: 980 745
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 188 1672 1725
Size: 188 1484 53
Fragments arranged by size:
1484 188 53
BsrBI CCG'CTC
Cuts at: 0 426 624 1725
Size: 426 198 1101
Fragments arranged by size:
1101 426 198
BsrDI GCAATG_nn'
Cuts at: 0 1062 1417 1725
Size: 1062 355 308
Fragments arranged by size:
1062 355 308
BsrFI r'CCGG_y
Cuts at: 0 279 455 509 513 693 829 1399 1725
Size: 279 176 54 4 180 136 570 326
Fragments arranged by size:
570 326 279 180 176 136 54 4
BstBI TT'CG_AA
Cuts at: 0 821 923 1725
Size: 821 102 802
Fragments arranged by size:
821 802 102
BstEII G'GTnAC_C
Cuts at: 0 1317 1725
Size: 1317 408
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1077 1725
Size: 1077 648
BstYI r'GATC_y
Cuts at: 0 548 1154 1381 1725
Size: 548 606 227 344
Fragments arranged by size:
606 548 344 227
Bsu36I CC'TnA_GG
Cuts at: 0 1087 1725
Size: 1087 638
BtgI C'CryG_G
Cuts at: 0 111 161 284 578 1725
Size: 111 50 123 294 1147
Fragments arranged by size:
1147 294 123 111 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 690 1380 1398 1725
Size: 690 690 18 327
Fragments arranged by size:
690 690 327 18
BtsI GCAGTG_nn'
Cuts at: 0 274 1319 1655 1725
Size: 274 1045 336 70
Fragments arranged by size:
1045 336 274 70
DraIII CAC_nnn'GTG
Cuts at: 0 1356 1725
Size: 1356 369
EaeI y'GGCC_r
Cuts at: 0 197 281 511 1001 1040 1079 1725
Size: 197 84 230 490 39 39 646
Fragments arranged by size:
646 490 230 197 84 39 39
EagI C'GGCC_G
Cuts at: 0 281 511 1725
Size: 281 230 1214
Fragments arranged by size:
1214 281 230
EarI CTCTTCn'nnn_
Cuts at: 0 673 1475 1533 1592 1725
Size: 673 802 58 59 133
Fragments arranged by size:
802 673 133 59 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 496 1087 1725
Size: 496 591 638
Fragments arranged by size:
638 591 496
EcoICRI GAG'CTC
Cuts at: 0 686 1542 1725
Size: 686 856 183
Fragments arranged by size:
856 686 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 376 778 849 1192 1221 1259 1514
Size: 205 171 402 71 343 29 38 255
Cuts at: 1514 1725
Size: 211
Fragments arranged by size:
402 343 255 211 205 171 71 38
29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 1725
Size: 613 1112
EcoO109I rG'GnC_Cy
Cuts at: 0 212 213 848 1725
Size: 212 1 635 877
Fragments arranged by size:
877 635 212 1
FseI GG_CCGG'CC
Cuts at: 0 697 1725
Size: 697 1028
HaeII r_GCGC'y
Cuts at: 0 12 75 654 1622 1725
Size: 12 63 579 968 103
Fragments arranged by size:
968 579 103 63 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 1187 1219 1725
Size: 523 32 632 32 506
Fragments arranged by size:
632 523 506 32 32
HincII GTy'rAC
Cuts at: 0 519 1698 1725
Size: 519 1179 27
Fragments arranged by size:
1179 519 27
KpnI G_GTAC'C
Cuts at: 0 1034 1725
Size: 1034 691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1466 1559 1725
Size: 1466 93 166
Fragments arranged by size:
1466 166 93
MscI TGG'CCA
Cuts at: 0 1003 1081 1725
Size: 1003 78 644
Fragments arranged by size:
1003 644 78
MslI CAynn'nnrTG
Cuts at: 0 103 160 1501 1725
Size: 103 57 1341 224
Fragments arranged by size:
1341 224 103 57
MspA1I CmG'CkG
Cuts at: 0 86 264 286 354 564 1725
Size: 86 178 22 68 210 1161
Fragments arranged by size:
1161 210 178 86 68 22
NaeI GCC'GGC
Cuts at: 0 281 511 695 831 1725
Size: 281 230 184 136 894
Fragments arranged by size:
894 281 230 184 136
NcoI C'CATG_G
Cuts at: 0 578 1725
Size: 578 1147
NgoMIV G'CCGG_C
Cuts at: 0 279 509 693 829 1725
Size: 279 230 184 136 896
Fragments arranged by size:
896 279 230 184 136
NruI TCG'CGA
Cuts at: 0 972 1725
Size: 972 753
NspI r_CATG'y
Cuts at: 0 1500 1725
Size: 1500 225
PflMI CCAn_nnn'nTGG
Cuts at: 0 102 183 1725
Size: 102 81 1542
Fragments arranged by size:
1542 102 81
PfoI T'CCnGG_A
Cuts at: 0 1292 1725
Size: 1292 433
PmlI CAC'GTG
Cuts at: 0 1503 1725
Size: 1503 222
PpuMI rG'GwC_Cy
Cuts at: 0 848 1725
Size: 848 877
PspOMI G'GGCC_C
Cuts at: 0 212 1725
Size: 212 1513
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 100 132 1725
Size: 100 32 1593
Fragments arranged by size:
1593 100 32
PstI C_TGCA'G
Cuts at: 0 1154 1725
Size: 1154 571
PvuII CAG'CTG
Cuts at: 0 354 564 1725
Size: 354 210 1161
Fragments arranged by size:
1161 354 210
SacI G_AGCT'C
Cuts at: 0 688 1544 1725
Size: 688 856 181
Fragments arranged by size:
856 688 181
SacII CC_GC'GG
Cuts at: 0 287 1725
Size: 287 1438
SanDI GG'GwC_CC
Cuts at: 0 848 1725
Size: 848 877
SapI GCTCTTCn'nnn_
Cuts at: 0 673 1475 1725
Size: 673 802 250
Fragments arranged by size:
802 673 250
ScaI AGT'ACT
Cuts at: 0 439 1725
Size: 439 1286
SfcI C'TryA_G
Cuts at: 0 1150 1725
Size: 1150 575
SgrAI Cr'CCGG_yG
Cuts at: 0 1399 1725
Size: 1399 326
SmaI CCC'GGG
Cuts at: 0 171 700 1725
Size: 171 529 1025
Fragments arranged by size:
1025 529 171
SmlI C'TyrA_G
Cuts at: 0 221 1725
Size: 221 1504
StyI C'CwwG_G
Cuts at: 0 578 1142 1725
Size: 578 564 583
Fragments arranged by size:
583 578 564
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1367 1725
Size: 1367 358
TatI w'GTAC_w
Cuts at: 0 437 1493 1725
Size: 437 1056 232
Fragments arranged by size:
1056 437 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 886 918 1725
Size: 886 32 807
Fragments arranged by size:
886 807 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1188 1329 1725
Size: 1188 141 396
Fragments arranged by size:
1188 396 141
XmaI C'CCGG_G
Cuts at: 0 169 698 1725
Size: 169 529 1027
Fragments arranged by size:
1027 529 169
ZraI GAC'GTC
Cuts at: 0 806 1725
Size: 806 919
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AleI ApaI
AvaI BanI BanII BbsI BbvCI BcgI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NruI NspI PflMI PfoI PmlI PpuMI PspOMI PsrI
PstI PvuII SacI SacII SanDI SapI ScaI SfcI
SgrAI SmaI SmlI StyI TaqII TatI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AhdI AlfI AloI AlwNI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BciVI BclI BfrBI BglI BglII BlpI BmtI
BplI BsaI BsaBI BsaWI BsiWI BsmI BspEI BsrGI
BssHII BssSI BstAPI BstZ17I ClaI CspCI DraI DrdI
EcoRI EcoRV FalI FspI FspAI HindIII HpaI KasI
MfeI MluI NarI NdeI NheI NotI NsiI PacI
PasI PciI PmeI PpiI PshAI PsiI PspXI PvuI
RsrII SalI SbfI SexAI SfiI SfoI SgfI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmnI