Restriction map for
pF1KB9879
(Linear) (Six-base) MAPSORT of: KIBB9879.seq Check: 8449 from: 1 to: 1752
KIBB9879.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 835 1297 1752
Size: 66 769 462 455
Fragments arranged by size:
769 462 455 66
AflII C'TTAA_G
Cuts at: 0 214 1752
Size: 214 1538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 193 626 1752
Size: 154 39 433 1126
Fragments arranged by size:
1126 433 154 39
AlwNI CAG_nnn'CTG
Cuts at: 0 514 699 1752
Size: 514 185 1053
Fragments arranged by size:
1053 514 185
ApaI G_GGCC'C
Cuts at: 0 206 263 1497 1618 1752
Size: 206 57 1234 121 134
Fragments arranged by size:
1234 206 134 121 57
AvaI C'yCGr_G
Cuts at: 0 184 199 1153 1592 1638 1752
Size: 184 15 954 439 46 114
Fragments arranged by size:
954 439 184 114 46 15
BanI G'GyrC_C
Cuts at: 0 543 671 1122 1218 1351 1560 1752
Size: 543 128 451 96 133 209 192
Fragments arranged by size:
543 451 209 192 133 128 96
BanII G_rGCy'C
Cuts at: 0 206 263 1107 1497 1618 1752
Size: 206 57 844 390 121 134
Fragments arranged by size:
844 390 206 134 121 57
BbeI G_GCGC'C
Cuts at: 0 675 1126 1355 1752
Size: 675 451 229 397
Fragments arranged by size:
675 451 397 229
BbsI GAAGACnn'nnnn_
Cuts at: 0 1538 1752
Size: 1538 214
BbvCI CC'TCA_GC
Cuts at: 0 440 1752
Size: 440 1312
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 766 800 1752
Size: 766 34 952
Fragments arranged by size:
952 766 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 793 1752
Size: 793 959
BglI GCCn_nnn'nGGC
Cuts at: 0 492 1078 1752
Size: 492 586 674
Fragments arranged by size:
674 586 492
Bme1580I G_kGCm'C
Cuts at: 0 206 263 1259 1497 1555 1565 1618 1657
Size: 206 57 996 238 58 10 53 39
Cuts at: 1657 1679 1752
Size: 22 73
Fragments arranged by size:
996 238 206 73 58 57 53 39
22 10
BmrI ACTGGGnnnn_n'
Cuts at: 0 1221 1487 1752
Size: 1221 266 265
Fragments arranged by size:
1221 266 265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 1752
Size: 210 1542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 779 1739 1752
Size: 403 376 960 13
Fragments arranged by size:
960 403 376 13
Bpu10I CC'TnA_GC
Cuts at: 0 440 1752
Size: 440 1312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 573 1752
Size: 148 425 1179
Fragments arranged by size:
1179 425 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 1237 1752
Size: 1237 515
BsaAI yAC'GTr
Cuts at: 0 177 1752
Size: 177 1575
BsaHI Gr'CG_yC
Cuts at: 0 4 16 672 863 1010 1123 1352 1752
Size: 4 12 656 191 147 113 229 400
Fragments arranged by size:
656 400 229 191 147 113 12 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 1207 1359 1659 1752
Size: 442 765 152 300 93
Fragments arranged by size:
765 442 300 152 93
BseYI C'CCAG_C
Cuts at: 0 263 284 290 417 987 1052 1174 1379
Size: 263 21 6 127 570 65 122 205
Cuts at: 1379 1391 1752
Size: 12 361
Fragments arranged by size:
570 361 263 205 127 122 65 21
12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1364 1536 1752
Size: 31 1333 172 216
Fragments arranged by size:
1333 216 172 31
BsiEI CG_ry'CG
Cuts at: 0 1622 1752
Size: 1622 130
BsiHKAI G_wGCw'C
Cuts at: 0 182 1752
Size: 182 1570
BsmI GAATG_Cn'
Cuts at: 0 615 1752
Size: 615 1137
Bsp1286I G_dGCh'C
Cuts at: 0 182 206 263 1107 1259 1497 1555 1565
Size: 182 24 57 844 152 238 58 10
Cuts at: 1565 1618 1657 1679 1752
Size: 53 39 22 73
Fragments arranged by size:
844 238 182 152 73 58 57 53
39 24 22 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 1752
Size: 56 1696
BsrBI CCG'CTC
Cuts at: 0 194 1524 1643 1752
Size: 194 1330 119 109
Fragments arranged by size:
1330 194 119 109
BsrDI GCAATG_nn'
Cuts at: 0 855 1265 1752
Size: 855 410 487
Fragments arranged by size:
855 487 410
BsrFI r'CCGG_y
Cuts at: 0 304 859 959 1068 1752
Size: 304 555 100 109 684
Fragments arranged by size:
684 555 304 109 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 237 417 1752
Size: 237 180 1335
Fragments arranged by size:
1335 237 180
BtgI C'CryG_G
Cuts at: 0 61 68 434 1037 1373 1752
Size: 61 7 366 603 336 379
Fragments arranged by size:
603 379 366 336 61 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1381 1752
Size: 1381 371
BtsI GCAGTG_nn'
Cuts at: 0 515 689 878 1752
Size: 515 174 189 874
Fragments arranged by size:
874 515 189 174
ClaI AT'CG_AT
Cuts at: 0 146 1752
Size: 146 1606
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1695 1730 1752
Size: 1695 35 22
Fragments arranged by size:
1695 35 22
DraIII CAC_nnn'GTG
Cuts at: 0 1215 1752
Size: 1215 537
EaeI y'GGCC_r
Cuts at: 0 224 407 1286 1619 1752
Size: 224 183 879 333 133
Fragments arranged by size:
879 333 224 183 133
EagI C'GGCC_G
Cuts at: 0 1619 1752
Size: 1619 133
EarI CTCTTCn'nnn_
Cuts at: 0 577 826 1752
Size: 577 249 926
Fragments arranged by size:
926 577 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 699 1337 1752
Size: 699 638 415
Fragments arranged by size:
699 638 415
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 403 779 835 1297 1739 1752
Size: 66 337 376 56 462 442 13
Fragments arranged by size:
462 442 376 337 66 56 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1322 1752
Size: 1322 430
EcoO109I rG'GnC_Cy
Cuts at: 0 203 234 259 260 398 455 1044 1314
Size: 203 31 25 1 138 57 589 270
Cuts at: 1314 1333 1493 1494 1614 1746 1752
Size: 19 160 1 120 132 6
Fragments arranged by size:
589 270 203 160 138 132 120 57
31 25 19 6 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1596 1752
Size: 1596 156
HaeII r_GCGC'y
Cuts at: 0 675 706 1126 1355 1752
Size: 675 31 420 229 397
Fragments arranged by size:
675 420 397 229 31
KasI G'GCGC_C
Cuts at: 0 671 1122 1351 1752
Size: 671 451 229 401
Fragments arranged by size:
671 451 401 229
MscI TGG'CCA
Cuts at: 0 409 1752
Size: 409 1343
MspA1I CmG'CkG
Cuts at: 0 267 505 987 1039 1240 1441 1460 1472
Size: 267 238 482 52 201 201 19 12
Cuts at: 1472 1659 1752
Size: 187 93
Fragments arranged by size:
482 267 238 201 201 187 93 52
19 12
NaeI GCC'GGC
Cuts at: 0 861 961 1070 1752
Size: 861 100 109 682
Fragments arranged by size:
861 682 109 100
NarI GG'CG_CC
Cuts at: 0 672 1123 1352 1752
Size: 672 451 229 400
Fragments arranged by size:
672 451 400 229
NcoI C'CATG_G
Cuts at: 0 68 1752
Size: 68 1684
NgoMIV G'CCGG_C
Cuts at: 0 859 959 1068 1752
Size: 859 100 109 684
Fragments arranged by size:
859 684 109 100
PasI CC'CwG_GG
Cuts at: 0 490 1296 1489 1679 1752
Size: 490 806 193 190 73
Fragments arranged by size:
806 490 193 190 73
PflMI CCAn_nnn'nTGG
Cuts at: 0 74 1752
Size: 74 1678
PfoI T'CCnGG_A
Cuts at: 0 795 1111 1752
Size: 795 316 641
Fragments arranged by size:
795 641 316
PmlI CAC'GTG
Cuts at: 0 177 1752
Size: 177 1575
PpuMI rG'GwC_Cy
Cuts at: 0 234 398 1314 1746 1752
Size: 234 164 916 432 6
Fragments arranged by size:
916 432 234 164 6
PspOMI G'GGCC_C
Cuts at: 0 202 259 1493 1614 1752
Size: 202 57 1234 121 138
Fragments arranged by size:
1234 202 138 121 57
PstI C_TGCA'G
Cuts at: 0 510 1365 1752
Size: 510 855 387
Fragments arranged by size:
855 510 387
PvuII CAG'CTG
Cuts at: 0 267 505 987 1752
Size: 267 238 482 765
Fragments arranged by size:
765 482 267 238
RsrII CG'GwC_CG
Cuts at: 0 126 1752
Size: 126 1626
SacII CC_GC'GG
Cuts at: 0 1040 1752
Size: 1040 712
SanDI GG'GwC_CC
Cuts at: 0 398 1746 1752
Size: 398 1348 6
Fragments arranged by size:
1348 398 6
SapI GCTCTTCn'nnn_
Cuts at: 0 826 1752
Size: 826 926
SfcI C'TryA_G
Cuts at: 0 506 1361 1752
Size: 506 855 391
Fragments arranged by size:
855 506 391
SfoI GGC'GCC
Cuts at: 0 673 1124 1353 1752
Size: 673 451 229 399
Fragments arranged by size:
673 451 399 229
SgrAI Cr'CCGG_yG
Cuts at: 0 859 1752
Size: 859 893
SmaI CCC'GGG
Cuts at: 0 201 1594 1752
Size: 201 1393 158
Fragments arranged by size:
1393 201 158
SmlI C'TyrA_G
Cuts at: 0 163 214 552 1752
Size: 163 51 338 1200
Fragments arranged by size:
1200 338 163 51
SrfI GCCC'GGGC
Cuts at: 0 201 1752
Size: 201 1551
StuI AGG'CCT
Cuts at: 0 533 1752
Size: 533 1219
StyI C'CwwG_G
Cuts at: 0 68 230 1310 1752
Size: 68 162 1080 442
Fragments arranged by size:
1080 442 162 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1203 1323 1552 1752
Size: 1203 120 229 200
Fragments arranged by size:
1203 229 200 120
TatI w'GTAC_w
Cuts at: 0 386 1631 1752
Size: 386 1245 121
Fragments arranged by size:
1245 386 121
Tth111I GACn'n_nGTC
Cuts at: 0 1009 1752
Size: 1009 743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 234 1752
Size: 234 1518
XmaI C'CCGG_G
Cuts at: 0 199 1592 1752
Size: 199 1393 160
Fragments arranged by size:
1393 199 160
XmnI GAAnn'nnTTC
Cuts at: 0 820 1752
Size: 820 932
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstXI BtgI BtgZI BtsI ClaI CspCI
DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII KasI MscI MspA1I NaeI NarI NcoI
NgoMIV PasI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII RsrII SacII SanDI SapI SfcI SfoI SgrAI
SmaI SmlI SrfI StuI StyI TaqII TatI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BclI BfrBI BglII BlpI BmgBI
BmtI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I DraI DrdI EcoICRI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MslI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI SacI SalI SbfI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SspI SwaI TstI XbaI XhoI
ZraI