Restriction map for
pF1KB9885
(Linear) (Six-base) MAPSORT of: KIBB9885.seq Check: 4470 from: 1 to: 1959
KIBB9885.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1026 1959
Size: 1026 933
AccI GT'mk_AC
Cuts at: 0 1182 1580 1959
Size: 1182 398 379
Fragments arranged by size:
1182 398 379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 653 1959
Size: 514 139 1306
Fragments arranged by size:
1306 514 139
AflII C'TTAA_G
Cuts at: 0 841 1959
Size: 841 1118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1326 1959
Size: 1326 633
AlwNI CAG_nnn'CTG
Cuts at: 0 141 940 1320 1890 1959
Size: 141 799 380 570 69
Fragments arranged by size:
799 570 380 141 69
ApoI r'AATT_y
Cuts at: 0 1549 1793 1959
Size: 1549 244 166
Fragments arranged by size:
1549 244 166
AvaI C'yCGr_G
Cuts at: 0 809 1959
Size: 809 1150
AvrII C'CTAG_G
Cuts at: 0 1006 1959
Size: 1006 953
BanI G'GyrC_C
Cuts at: 0 1573 1959
Size: 1573 386
BanII G_rGCy'C
Cuts at: 0 272 365 794 818 839 985 1959
Size: 272 93 429 24 21 146 974
Fragments arranged by size:
974 429 272 146 93 24 21
BbvCI CC'TCA_GC
Cuts at: 0 425 1568 1959
Size: 425 1143 391
Fragments arranged by size:
1143 425 391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1666 1700 1959
Size: 1666 34 259
Fragments arranged by size:
1666 259 34
BglI GCCn_nnn'nGGC
Cuts at: 0 928 1235 1959
Size: 928 307 724
Fragments arranged by size:
928 724 307
BglII A'GATC_T
Cuts at: 0 1272 1922 1959
Size: 1272 650 37
Fragments arranged by size:
1272 650 37
BlpI GC'TnA_GC
Cuts at: 0 271 1255 1405 1959
Size: 271 984 150 554
Fragments arranged by size:
984 554 271 150
Bme1580I G_kGCm'C
Cuts at: 0 1576 1959
Size: 1576 383
BmrI ACTGGGnnnn_n'
Cuts at: 0 1226 1959
Size: 1226 733
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1062 1959
Size: 1062 897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 538 781 1085 1630 1657 1959
Size: 163 375 243 304 545 27 302
Fragments arranged by size:
545 375 304 302 243 163 27
Bpu10I CC'TnA_GC
Cuts at: 0 425 1568 1596 1959
Size: 425 1143 28 363
Fragments arranged by size:
1143 425 363 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1339 1959
Size: 1057 282 620
Fragments arranged by size:
1057 620 282
BsaAI yAC'GTr
Cuts at: 0 1134 1959
Size: 1134 825
BsaHI Gr'CG_yC
Cuts at: 0 315 435 1959
Size: 315 120 1524
Fragments arranged by size:
1524 315 120
BsaWI w'CCGG_w
Cuts at: 0 1854 1959
Size: 1854 105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 330 360 1959
Size: 330 30 1599
Fragments arranged by size:
1599 330 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 586 679 778 793 796 1280 1959
Size: 442 144 93 99 15 3 484 679
Fragments arranged by size:
679 484 442 144 99 93 15 3
BseYI C'CCAG_C
Cuts at: 0 221 265 1680 1959
Size: 221 44 1415 279
Fragments arranged by size:
1415 279 221 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 718 1959
Size: 94 624 1241
Fragments arranged by size:
1241 624 94
BsiHKAI G_wGCw'C
Cuts at: 0 794 818 839 1631 1959
Size: 794 24 21 792 328
Fragments arranged by size:
794 792 328 24 21
BsmI GAATG_Cn'
Cuts at: 0 61 1959
Size: 61 1898
Bsp1286I G_dGCh'C
Cuts at: 0 272 365 794 818 839 985 1576 1631
Size: 272 93 429 24 21 146 591 55
Cuts at: 1631 1959
Size: 328
Fragments arranged by size:
591 429 328 272 146 93 55 24
21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 686 1026 1959
Size: 686 340 933
Fragments arranged by size:
933 686 340
BsrBI CCG'CTC
Cuts at: 0 432 1959
Size: 432 1527
BsrDI GCAATG_nn'
Cuts at: 0 686 1310 1730 1911 1959
Size: 686 624 420 181 48
Fragments arranged by size:
686 624 420 181 48
BsrGI T'GTAC_A
Cuts at: 0 400 1959
Size: 400 1559
BssSI C'ACGA_G
Cuts at: 0 1754 1959
Size: 1754 205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 562 1959
Size: 69 493 1397
Fragments arranged by size:
1397 493 69
BstYI r'GATC_y
Cuts at: 0 1272 1922 1959
Size: 1272 650 37
Fragments arranged by size:
1272 650 37
BstZ17I GTA'TAC
Cuts at: 0 1183 1959
Size: 1183 776
Bsu36I CC'TnA_GG
Cuts at: 0 1037 1808 1877 1959
Size: 1037 771 69 82
Fragments arranged by size:
1037 771 82 69
BtgI C'CryG_G
Cuts at: 0 923 1167 1414 1959
Size: 923 244 247 545
Fragments arranged by size:
923 545 247 244
BtsI GCAGTG_nn'
Cuts at: 0 794 1066 1377 1959
Size: 794 272 311 582
Fragments arranged by size:
794 582 311 272
DraIII CAC_nnn'GTG
Cuts at: 0 1383 1959
Size: 1383 576
DrdI GACnn_nn'nnGTC
Cuts at: 0 1850 1959
Size: 1850 109
EaeI y'GGCC_r
Cuts at: 0 117 155 393 728 1374 1440 1959
Size: 117 38 238 335 646 66 519
Fragments arranged by size:
646 519 335 238 117 66 38
EarI CTCTTCn'nnn_
Cuts at: 0 80 1959
Size: 80 1879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 510 1210 1959
Size: 510 700 749
Fragments arranged by size:
749 700 510
EcoICRI GAG'CTC
Cuts at: 0 792 816 837 1959
Size: 792 24 21 1122
Fragments arranged by size:
1122 792 24 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 514 538 653 781 1085 1630 1657
Size: 163 351 24 115 128 304 545 27
Cuts at: 1657 1959
Size: 302
Fragments arranged by size:
545 351 304 302 163 128 115 27
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1392 1959
Size: 1392 567
EcoO109I rG'GnC_Cy
Cuts at: 0 525 1010 1033 1666 1959
Size: 525 485 23 633 293
Fragments arranged by size:
633 525 485 293 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 522 1959
Size: 522 1437
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 1486 1518 1959
Size: 219 32 1235 32 441
Fragments arranged by size:
1235 441 219 32 32
HincII GTy'rAC
Cuts at: 0 458 903 1959
Size: 458 445 1056
Fragments arranged by size:
1056 458 445
HindIII A'AGCT_T
Cuts at: 0 103 1257 1474 1959
Size: 103 1154 217 485
Fragments arranged by size:
1154 485 217 103
HpaI GTT'AAC
Cuts at: 0 458 903 1959
Size: 458 445 1056
Fragments arranged by size:
1056 458 445
MscI TGG'CCA
Cuts at: 0 119 157 395 730 1376 1442 1959
Size: 119 38 238 335 646 66 517
Fragments arranged by size:
646 517 335 238 119 66 38
MslI CAynn'nnrTG
Cuts at: 0 67 1744 1959
Size: 67 1677 215
Fragments arranged by size:
1677 215 67
MspA1I CmG'CkG
Cuts at: 0 93 179 225 265 424 492 772 1029
Size: 93 86 46 40 159 68 280 257
Cuts at: 1029 1404 1523 1680 1727 1887 1959
Size: 375 119 157 47 160 72
Fragments arranged by size:
375 280 257 160 159 157 119 93
86 72 68 47 46 40
NcoI C'CATG_G
Cuts at: 0 923 1414 1959
Size: 923 491 545
Fragments arranged by size:
923 545 491
NspI r_CATG'y
Cuts at: 0 1743 1959
Size: 1743 216
PasI CC'CwG_GG
Cuts at: 0 1590 1959
Size: 1590 369
PflMI CCAn_nnn'nTGG
Cuts at: 0 141 1383 1959
Size: 141 1242 576
Fragments arranged by size:
1242 576 141
PfoI T'CCnGG_A
Cuts at: 0 41 554 584 1101 1959
Size: 41 513 30 517 858
Fragments arranged by size:
858 517 513 41 30
PpuMI rG'GwC_Cy
Cuts at: 0 525 1033 1959
Size: 525 508 926
Fragments arranged by size:
926 525 508
PshAI GACnn'nnGTC
Cuts at: 0 1781 1959
Size: 1781 178
PstI C_TGCA'G
Cuts at: 0 9 491 1959
Size: 9 482 1468
Fragments arranged by size:
1468 482 9
PvuII CAG'CTG
Cuts at: 0 93 225 265 424 772 1404 1523 1680
Size: 93 132 40 159 348 632 119 157
Cuts at: 1680 1887 1959
Size: 207 72
Fragments arranged by size:
632 348 207 159 157 132 119 93
72 40
SacI G_AGCT'C
Cuts at: 0 794 818 839 1959
Size: 794 24 21 1120
Fragments arranged by size:
1120 794 24 21
SapI GCTCTTCn'nnn_
Cuts at: 0 80 1959
Size: 80 1879
ScaI AGT'ACT
Cuts at: 0 1154 1959
Size: 1154 805
SfcI C'TryA_G
Cuts at: 0 5 487 1661 1821 1926 1959
Size: 5 482 1174 160 105 33
Fragments arranged by size:
1174 482 160 105 33 5
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 928 1959
Size: 928 1031
SmlI C'TyrA_G
Cuts at: 0 841 1072 1354 1959
Size: 841 231 282 605
Fragments arranged by size:
841 605 282 231
SpeI A'CTAG_T
Cuts at: 0 1155 1959
Size: 1155 804
StuI AGG'CCT
Cuts at: 0 1601 1959
Size: 1601 358
StyI C'CwwG_G
Cuts at: 0 506 521 923 1006 1263 1367 1414 1959
Size: 506 15 402 83 257 104 47 545
Fragments arranged by size:
545 506 402 257 104 83 47 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 290 1603 1959
Size: 290 1313 356
Fragments arranged by size:
1313 356 290
TatI w'GTAC_w
Cuts at: 0 400 1152 1959
Size: 400 752 807
Fragments arranged by size:
807 752 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1493 1525 1959
Size: 1493 32 434
Fragments arranged by size:
1493 434 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 388 654 1321 1639 1959
Size: 388 266 667 318 320
Fragments arranged by size:
667 388 320 318 266
XmnI GAAnn'nnTTC
Cuts at: 0 613 1947 1959
Size: 613 1334 12
Fragments arranged by size:
1334 613 12
Enzymes that do cut:
AarI AccI AcuI AflII AlfI AlwNI ApoI AvaI
AvrII BanI BanII BbvCI BcgI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrGI BssSI BstXI BstYI BstZ17I
Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HindIII
HpaI MscI MslI MspA1I NcoI NspI PasI PflMI
PfoI PpuMI PshAI PstI PvuII SacI SapI ScaI
SfcI SfiI SmlI SpeI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI BaeI BamHI
BbeI BbsI BciVI BclI BfrBI BmgBI BmtI BsaI
BsaBI BsiEI BsiWI BsmBI BspEI BspHI BsrFI BssHII
BstAPI BstBI BstEII BtgZI ClaI CspCI DraI EagI
EcoRI EcoRV FseI FspI FspAI HaeII KasI KpnI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI