Restriction map for
pF1KB9891
(Linear) (Six-base) MAPSORT of: KIBB9891.seq Check: 7347 from: 1 to: 1401
KIBB9891.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1361 1401
Size: 1165 196 40
Fragments arranged by size:
1165 196 40
AflIII A'CryG_T
Cuts at: 0 372 1401
Size: 372 1029
AlwNI CAG_nnn'CTG
Cuts at: 0 811 1401
Size: 811 590
ApaLI G'TGCA_C
Cuts at: 0 1233 1401
Size: 1233 168
ApoI r'AATT_y
Cuts at: 0 248 918 1124 1165 1401
Size: 248 670 206 41 236
Fragments arranged by size:
670 248 236 206 41
BamHI G'GATC_C
Cuts at: 0 1369 1401
Size: 1369 32
BanI G'GyrC_C
Cuts at: 0 1103 1401
Size: 1103 298
BanII G_rGCy'C
Cuts at: 0 1078 1401
Size: 1078 323
BciVI GTATCCnnnnn_n'
Cuts at: 0 282 360 1401
Size: 282 78 1041
Fragments arranged by size:
1041 282 78
BfrBI ATG'CAT
Cuts at: 0 874 1401
Size: 874 527
BglII A'GATC_T
Cuts at: 0 1148 1401
Size: 1148 253
Bme1580I G_kGCm'C
Cuts at: 0 542 1237 1401
Size: 542 695 164
Fragments arranged by size:
695 542 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 328 512 719 1401
Size: 95 233 184 207 682
Fragments arranged by size:
682 233 207 184 95
Bpu10I CC'TnA_GC
Cuts at: 0 936 1401
Size: 936 465
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 412 1374 1401
Size: 202 210 962 27
Fragments arranged by size:
962 210 202 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 332 1401
Size: 332 1069
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 648 678 717 747 1284 1314 1401
Size: 648 30 39 30 537 30 87
Fragments arranged by size:
648 537 87 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 92 104 1016 1401
Size: 92 12 912 385
Fragments arranged by size:
912 385 92 12
BseYI C'CCAG_C
Cuts at: 0 45 618 1401
Size: 45 573 783
Fragments arranged by size:
783 573 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1090 1401
Size: 1090 311
BsiHKAI G_wGCw'C
Cuts at: 0 1237 1401
Size: 1237 164
BsmBI CGTCTCn'nnnn_
Cuts at: 0 346 1401
Size: 346 1055
Bsp1286I G_dGCh'C
Cuts at: 0 542 1078 1237 1401
Size: 542 536 159 164
Fragments arranged by size:
542 536 164 159
BspHI T'CATG_A
Cuts at: 0 420 1401
Size: 420 981
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 819 1401
Size: 819 582
BstYI r'GATC_y
Cuts at: 0 554 1148 1369 1401
Size: 554 594 221 32
Fragments arranged by size:
594 554 221 32
BtgI C'CryG_G
Cuts at: 0 968 1401
Size: 968 433
BtsI GCAGTG_nn'
Cuts at: 0 137 1401
Size: 137 1264
EarI CTCTTCn'nnn_
Cuts at: 0 31 993 1181 1401
Size: 31 962 188 220
Fragments arranged by size:
962 220 188 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 101 560 1401
Size: 101 459 841
Fragments arranged by size:
841 459 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 328 512 719 1165 1361 1401
Size: 95 233 184 207 446 196 40
Fragments arranged by size:
446 233 207 196 184 95 40
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1319 1401
Size: 1319 82
HaeII r_GCGC'y
Cuts at: 0 399 1401
Size: 399 1002
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 715 747 1401
Size: 715 32 654
Fragments arranged by size:
715 654 32
HincII GTy'rAC
Cuts at: 0 957 1401
Size: 957 444
HpaI GTT'AAC
Cuts at: 0 957 1401
Size: 957 444
MfeI C'AATT_G
Cuts at: 0 434 1080 1401
Size: 434 646 321
Fragments arranged by size:
646 434 321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 837 1325 1401
Size: 837 488 76
Fragments arranged by size:
837 488 76
MslI CAynn'nnrTG
Cuts at: 0 819 1243 1325 1401
Size: 819 424 82 76
Fragments arranged by size:
819 424 82 76
MspA1I CmG'CkG
Cuts at: 0 64 1401
Size: 64 1337
NsiI A_TGCA'T
Cuts at: 0 876 1401
Size: 876 525
NspI r_CATG'y
Cuts at: 0 376 1398 1401
Size: 376 1022 3
Fragments arranged by size:
1022 376 3
PciI A'CATG_T
Cuts at: 0 372 1401
Size: 372 1029
PflMI CCAn_nnn'nTGG
Cuts at: 0 667 1401
Size: 667 734
PfoI T'CCnGG_A
Cuts at: 0 666 1401
Size: 666 735
PvuII CAG'CTG
Cuts at: 0 64 1401
Size: 64 1337
SapI GCTCTTCn'nnn_
Cuts at: 0 1181 1401
Size: 1181 220
ScaI AGT'ACT
Cuts at: 0 1299 1401
Size: 1299 102
SexAI A'CCwGG_T
Cuts at: 0 322 1401
Size: 322 1079
SfcI C'TryA_G
Cuts at: 0 893 1336 1401
Size: 893 443 65
Fragments arranged by size:
893 443 65
SmlI C'TyrA_G
Cuts at: 0 181 427 1389 1401
Size: 181 246 962 12
Fragments arranged by size:
962 246 181 12
StuI AGG'CCT
Cuts at: 0 299 1401
Size: 299 1102
TatI w'GTAC_w
Cuts at: 0 262 1297 1401
Size: 262 1035 104
Fragments arranged by size:
1035 262 104
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 100 132 717 749 989 1021 1401
Size: 100 32 585 32 240 32 380
Fragments arranged by size:
585 380 240 100 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1150 1401
Size: 1150 251
Enzymes that do cut:
AcuI AflIII AlwNI ApaLI ApoI BamHI BanI BanII
BciVI BfrBI BglII Bme1580I BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BstYI BtgI BtsI EarI EciI Eco57MI FalI
HaeII Hin4I HincII HpaI MfeI MmeI MslI MspA1I
NsiI NspI PciI PflMI PfoI PvuII SapI ScaI
SexAI SfcI SmlI StuI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BbvCI BcgI BclI
BglI BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HindIII KasI KpnI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI ZraI