(Linear) (Six-base) MAPSORT of: KIBB9897.seq  Check: 298  from: 1  to: 1530



KIBB9897.orf

 With 182 enzymes: SgfI * 

                        March 30, 2011 17:32  ..

AccI GT'mk_AC

Cuts at:      0     769    1530 
   Size:        769     761
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     472     632    1530 
   Size:        260     212     160     898

  Fragments arranged by size: 

                 898     260     212     160
 
AflII C'TTAA_G

Cuts at:      0    1120    1530 
   Size:       1120     410
 
AflIII A'CryG_T

Cuts at:      0     553    1525    1530 
   Size:        553     972       5

  Fragments arranged by size: 

                 972     553       5
 
AhdI GACnn_n'nnGTC

Cuts at:      0     528    1530 
   Size:        528    1002
 
ApoI r'AATT_y

Cuts at:      0     151     270     645     976    1530 
   Size:        151     119     375     331     554

  Fragments arranged by size: 

                 554     375     331     151     119
 
AseI AT'TA_AT

Cuts at:      0    1105    1530 
   Size:       1105     425
 
BanI G'GyrC_C

Cuts at:      0     729    1530 
   Size:        729     801
 
BanII G_rGCy'C

Cuts at:      0    1246    1530 
   Size:       1246     284
 
BclI T'GATC_A

Cuts at:      0     822    1530 
   Size:        822     708
 
BglII A'GATC_T

Cuts at:      0     721    1116    1530 
   Size:        721     395     414

  Fragments arranged by size: 

                 721     414     395
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     919    1530 
   Size:        919     611
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     697     874    1530 
   Size:        697     177     656

  Fragments arranged by size: 

                 697     656     177
 
BsaAI yAC'GTr

Cuts at:      0     554    1530 
   Size:        554     976
 
BsaBI GATnn'nnATC

Cuts at:      0     872     914    1530 
   Size:        872      42     616

  Fragments arranged by size: 

                 872     616      42
 
Bsp1286I G_dGCh'C

Cuts at:      0    1246    1530 
   Size:       1246     284
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      10    1530 
   Size:         10    1520
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     193    1530 
   Size:        193    1337
 
BstYI r'GATC_y

Cuts at:      0     721    1116    1530 
   Size:        721     395     414

  Fragments arranged by size: 

                 721     414     395
 
BstZ17I GTA'TAC

Cuts at:      0     770    1530 
   Size:        770     760
 
Bsu36I CC'TnA_GG

Cuts at:      0    1445    1530 
   Size:       1445      85
 
BtgI C'CryG_G

Cuts at:      0     290    1530 
   Size:        290    1240
 
BtsI GCAGTG_nn'

Cuts at:      0     912    1334    1530 
   Size:        912     422     196

  Fragments arranged by size: 

                 912     422     196
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1061    1096    1530 
   Size:       1061      35     434

  Fragments arranged by size: 

                1061     434      35
 
EaeI y'GGCC_r

Cuts at:      0     467    1530 
   Size:        467    1063
 
EarI CTCTTCn'nnn_

Cuts at:      0     141    1014    1421    1530 
   Size:        141     873     407     109

  Fragments arranged by size: 

                 873     407     141     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     805    1530 
   Size:        805     725
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     472     632    1530 
   Size:        260     212     160     898

  Fragments arranged by size: 

                 898     260     212     160
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      95    1530 
   Size:         95    1435
 
EcoO109I rG'GnC_Cy

Cuts at:      0     100     992    1478    1530 
   Size:        100     892     486      52

  Fragments arranged by size: 

                 892     486     100      52
 
EcoRI G'AATT_C

Cuts at:      0     270     976    1530 
   Size:        270     706     554

  Fragments arranged by size: 

                 706     554     270
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     590    1530 
   Size:        590     940
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     514     546    1530 
   Size:        514      32     984

  Fragments arranged by size: 

                 984     514      32
 
MfeI C'AATT_G

Cuts at:      0     463    1530 
   Size:        463    1067
 
MslI CAynn'nnrTG

Cuts at:      0     845     970    1328    1530 
   Size:        845     125     358     202

  Fragments arranged by size: 

                 845     358     202     125
 
NcoI C'CATG_G

Cuts at:      0     290    1530 
   Size:        290    1240
 
NspI r_CATG'y

Cuts at:      0    1529    1530 
   Size:       1529       1
 
PciI A'CATG_T

Cuts at:      0    1525    1530 
   Size:       1525       5
 
PpuMI rG'GwC_Cy

Cuts at:      0     100     992    1478    1530 
   Size:        100     892     486      52

  Fragments arranged by size: 

                 892     486     100      52
 
PsiI TTA'TAA

Cuts at:      0     136     398    1530 
   Size:        136     262    1132

  Fragments arranged by size: 

                1132     262     136
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     821     853    1530 
   Size:        821      32     677

  Fragments arranged by size: 

                 821     677      32
 
ScaI AGT'ACT

Cuts at:      0      71    1530 
   Size:         71    1459
 
SfcI C'TryA_G

Cuts at:      0    1420    1530 
   Size:       1420     110
 
SmlI C'TyrA_G

Cuts at:      0     898    1120    1530 
   Size:        898     222     410

  Fragments arranged by size: 

                 898     410     222
 
SspI AAT'ATT

Cuts at:      0     834    1530 
   Size:        834     696
 
StuI AGG'CCT

Cuts at:      0     308    1530 
   Size:        308    1222
 
StyI C'CwwG_G

Cuts at:      0     290     995    1530 
   Size:        290     705     535

  Fragments arranged by size: 

                 705     535     290
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     747    1530 
   Size:        747     783
 
TatI w'GTAC_w

Cuts at:      0      69    1530 
   Size:         69    1461
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1265    1297    1530 
   Size:       1265      32     233

  Fragments arranged by size: 

                1265     233      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     389    1319    1530 
   Size:        389     930     211

  Fragments arranged by size: 

                 930     389     211
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII     AhdI     ApoI     AseI     BanI
    BanII     BclI    BglII    BpuEI     BsaI    BsaAI    BsaBI Bsp1286I
    BspMI   BstAPI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    Hin4I     MfeI     MslI     NcoI     NspI     PciI    PpuMI     PsiI
     PsrI     ScaI     SfcI     SmlI     SspI     StuI     StyI    TaqII
     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI