(Linear) (Six-base) MAPSORT of: KIBB9903.seq  Check: 1525  from: 1  to: 765



KIBB9903.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665     679     765 
   Size:        665      14      86

  Fragments arranged by size: 

                 665      86      14
 
AlwNI CAG_nnn'CTG

Cuts at:      0     258     553     765 
   Size:        258     295     212

  Fragments arranged by size: 

                 295     258     212
 
ApaI G_GGCC'C

Cuts at:      0     713     765 
   Size:        713      52
 
ApaLI G'TGCA_C

Cuts at:      0     500     765 
   Size:        500     265
 
ApoI r'AATT_y

Cuts at:      0      89     765 
   Size:         89     676
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     339     372     765 
   Size:        339      33     393

  Fragments arranged by size: 

                 393     339      33
 
BanII G_rGCy'C

Cuts at:      0     713     765 
   Size:        713      52
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     198     749     765 
   Size:        198     551      16

  Fragments arranged by size: 

                 551     198      16
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     367     765 
   Size:        367     398
 
BclI T'GATC_A

Cuts at:      0     249     765 
   Size:        249     516
 
Bme1580I G_kGCm'C

Cuts at:      0     504     713     765 
   Size:        504     209      52

  Fragments arranged by size: 

                 504     209      52
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     316     765 
   Size:        316     449
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     575     765 
   Size:        575     190
 
BsaWI w'CCGG_w

Cuts at:      0     369     765 
   Size:        369     396
 
BsiEI CG_ry'CG

Cuts at:      0      63     765 
   Size:         63     702
 
BsiHKAI G_wGCw'C

Cuts at:      0     504     765 
   Size:        504     261
 
BsmI GAATG_Cn'

Cuts at:      0     621     765 
   Size:        621     144
 
Bsp1286I G_dGCh'C

Cuts at:      0     504     713     765 
   Size:        504     209      52

  Fragments arranged by size: 

                 504     209      52
 
BssSI C'ACGA_G

Cuts at:      0     298     765 
   Size:        298     467
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     508     765 
   Size:        508     257
 
BstEII G'GTnAC_C

Cuts at:      0     372     765 
   Size:        372     393
 
BstYI r'GATC_y

Cuts at:      0     683     765 
   Size:        683      82
 
EarI CTCTTCn'nnn_

Cuts at:      0     725     765 
   Size:        725      40
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      31     765 
   Size:         31     734
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     575     665     679     765 
   Size:        575      90      14      86

  Fragments arranged by size: 

                 575      90      86      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     709     765 
   Size:        709      56
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     554     765 
   Size:        554     211
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     553     765 
   Size:        553     212
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     266     298     466     498     765 
   Size:        266      32     168      32     267

  Fragments arranged by size: 

                 267     266     168      32      32
 
PsiI TTA'TAA

Cuts at:      0     410     765 
   Size:        410     355
 
PspOMI G'GGCC_C

Cuts at:      0     709     765 
   Size:        709      56
 
PstI C_TGCA'G

Cuts at:      0     674     765 
   Size:        674      91
 
ScaI AGT'ACT

Cuts at:      0     431     765 
   Size:        431     334
 
SfcI C'TryA_G

Cuts at:      0     670     765 
   Size:        670      95
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     298     765 
   Size:        298     467
 
TatI w'GTAC_w

Cuts at:      0      41     429     765 
   Size:         41     388     336

  Fragments arranged by size: 

                 388     336      41
 
Tth111I GACn'n_nGTC

Cuts at:      0     748     765 
   Size:        748      17
 
XmnI GAAnn'nnTTC

Cuts at:      0     278     481     765 
   Size:        278     203     284

  Fragments arranged by size: 

                 284     278     203
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI    ApaLI     ApoI     BaeI    BanII     BbsI
    BciVI     BclI Bme1580I     BmrI     BpmI    BsaWI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BssSI   BstAPI   BstEII    BstYI     EarI     EciI
  Eco57MI EcoO109I     FalI    PflMI     PpiI     PsiI   PspOMI     PstI
     ScaI     SfcI    TaqII     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI     BbeI    BbvCI     BcgI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI    PpuMI    PshAI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TstI     XbaI     XcmI     XhoI     XmaI     ZraI