Restriction map for
pF1KB9908
(Linear) (Six-base) MAPSORT of: KIBB9908.seq Check: 7129 from: 1 to: 1767
KIBB9908.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1638 1767
Size: 1638 129
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 955 1650 1767
Size: 540 415 695 117
Fragments arranged by size:
695 540 415 117
AfeI AGC'GCT
Cuts at: 0 1231 1767
Size: 1231 536
AflIII A'CryG_T
Cuts at: 0 1085 1767
Size: 1085 682
AlwNI CAG_nnn'CTG
Cuts at: 0 396 711 1569 1767
Size: 396 315 858 198
Fragments arranged by size:
858 396 315 198
ApoI r'AATT_y
Cuts at: 0 321 1767
Size: 321 1446
AvaI C'yCGr_G
Cuts at: 0 7 44 145 940 1009 1213 1741 1767
Size: 7 37 101 795 69 204 528 26
Fragments arranged by size:
795 528 204 101 69 37 26 7
BanI G'GyrC_C
Cuts at: 0 188 1039 1767
Size: 188 851 728
Fragments arranged by size:
851 728 188
BanII G_rGCy'C
Cuts at: 0 8 1717 1767
Size: 8 1709 50
Fragments arranged by size:
1709 50 8
BbeI G_GCGC'C
Cuts at: 0 192 1767
Size: 192 1575
BbsI GAAGACnn'nnnn_
Cuts at: 0 858 1767
Size: 858 909
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 12 46 1127 1161 1767
Size: 12 34 1081 34 606
Fragments arranged by size:
1081 606 34 34 12
BglI GCCn_nnn'nGGC
Cuts at: 0 186 842 1767
Size: 186 656 925
Fragments arranged by size:
925 656 186
BglII A'GATC_T
Cuts at: 0 881 1767
Size: 881 886
BlpI GC'TnA_GC
Cuts at: 0 139 229 1767
Size: 139 90 1538
Fragments arranged by size:
1538 139 90
Bme1580I G_kGCm'C
Cuts at: 0 1010 1042 1317 1523 1767
Size: 1010 32 275 206 244
Fragments arranged by size:
1010 275 244 206 32
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 418 733 916 1402 1492 1767
Size: 85 333 315 183 486 90 275
Fragments arranged by size:
486 333 315 275 183 90 85
Bpu10I CC'TnA_GC
Cuts at: 0 457 1767
Size: 457 1310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 796 1767
Size: 22 774 971
Fragments arranged by size:
971 774 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 754 1543 1767
Size: 754 789 224
Fragments arranged by size:
789 754 224
BsaHI Gr'CG_yC
Cuts at: 0 189 1767
Size: 189 1578
BsaWI w'CCGG_w
Cuts at: 0 1059 1767
Size: 1059 708
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1540 1570 1767
Size: 1540 30 197
Fragments arranged by size:
1540 197 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1198 1231 1258 1767
Size: 1198 33 27 509
Fragments arranged by size:
1198 509 33 27
BseYI C'CCAG_C
Cuts at: 0 389 1365 1736 1767
Size: 389 976 371 31
Fragments arranged by size:
976 389 371 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 889 1616 1767
Size: 369 520 727 151
Fragments arranged by size:
727 520 369 151
BsiHKAI G_wGCw'C
Cuts at: 0 8 1334 1717 1767
Size: 8 1326 383 50
Fragments arranged by size:
1326 383 50 8
Bsp1286I G_dGCh'C
Cuts at: 0 8 1010 1042 1317 1334 1523 1717 1767
Size: 8 1002 32 275 17 189 194 50
Fragments arranged by size:
1002 275 194 189 50 32 17 8
BspEI T'CCGG_A
Cuts at: 0 1059 1767
Size: 1059 708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 911 1638 1767
Size: 911 727 129
Fragments arranged by size:
911 727 129
BsrBI CCG'CTC
Cuts at: 0 174 1194 1250 1767
Size: 174 1020 56 517
Fragments arranged by size:
1020 517 174 56
BsrGI T'GTAC_A
Cuts at: 0 923 1767
Size: 923 844
BssSI C'ACGA_G
Cuts at: 0 216 448 1767
Size: 216 232 1319
Fragments arranged by size:
1319 232 216
BstYI r'GATC_y
Cuts at: 0 707 881 1767
Size: 707 174 886
Fragments arranged by size:
886 707 174
Bsu36I CC'TnA_GG
Cuts at: 0 356 1767
Size: 356 1411
BtgI C'CryG_G
Cuts at: 0 686 963 1043 1767
Size: 686 277 80 724
Fragments arranged by size:
724 686 277 80
BtsI GCAGTG_nn'
Cuts at: 0 520 1317 1521 1767
Size: 520 797 204 246
Fragments arranged by size:
797 520 246 204
DrdI GACnn_nn'nnGTC
Cuts at: 0 1667 1767
Size: 1667 100
EaeI y'GGCC_r
Cuts at: 0 260 966 1767
Size: 260 706 801
Fragments arranged by size:
801 706 260
EarI CTCTTCn'nnn_
Cuts at: 0 122 1669 1705 1767
Size: 122 1547 36 62
Fragments arranged by size:
1547 122 62 36
EcoICRI GAG'CTC
Cuts at: 0 6 1715 1767
Size: 6 1709 52
Fragments arranged by size:
1709 52 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 418 540 733 916 955 1402 1492
Size: 85 333 122 193 183 39 447 90
Cuts at: 1492 1650 1767
Size: 158 117
Fragments arranged by size:
447 333 193 183 158 122 117 90
85 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 1273 1767
Size: 424 849 494
Fragments arranged by size:
849 494 424
EcoO109I rG'GnC_Cy
Cuts at: 0 340 360 406 900 930 1209 1278 1746
Size: 340 20 46 494 30 279 69 468
Cuts at: 1746 1767
Size: 21
Fragments arranged by size:
494 468 340 279 69 46 30 21
20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1371 1767
Size: 1371 396
HaeII r_GCGC'y
Cuts at: 0 192 694 1111 1233 1767
Size: 192 502 417 122 534
Fragments arranged by size:
534 502 417 192 122
KasI G'GCGC_C
Cuts at: 0 188 1767
Size: 188 1579
MspA1I CmG'CkG
Cuts at: 0 236 372 393 1767
Size: 236 136 21 1374
Fragments arranged by size:
1374 236 136 21
NarI GG'CG_CC
Cuts at: 0 189 1767
Size: 189 1578
NcoI C'CATG_G
Cuts at: 0 686 1767
Size: 686 1081
NspI r_CATG'y
Cuts at: 0 302 1006 1380 1767
Size: 302 704 374 387
Fragments arranged by size:
704 387 374 302
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 1529 1760 1767
Size: 396 1133 231 7
Fragments arranged by size:
1133 396 231 7
PfoI T'CCnGG_A
Cuts at: 0 248 425 710 1562 1767
Size: 248 177 285 852 205
Fragments arranged by size:
852 285 248 205 177
PpuMI rG'GwC_Cy
Cuts at: 0 340 360 406 900 930 1209 1278 1767
Size: 340 20 46 494 30 279 69 489
Fragments arranged by size:
494 489 340 279 69 46 30 20
PspXI vC'TCGA_Gb
Cuts at: 0 145 1767
Size: 145 1622
PstI C_TGCA'G
Cuts at: 0 515 1658 1698 1767
Size: 515 1143 40 69
Fragments arranged by size:
1143 515 69 40
PvuII CAG'CTG
Cuts at: 0 372 1767
Size: 372 1395
SacI G_AGCT'C
Cuts at: 0 8 1717 1767
Size: 8 1709 50
Fragments arranged by size:
1709 50 8
SanDI GG'GwC_CC
Cuts at: 0 406 1767
Size: 406 1361
SapI GCTCTTCn'nnn_
Cuts at: 0 122 1705 1767
Size: 122 1583 62
Fragments arranged by size:
1583 122 62
SfcI C'TryA_G
Cuts at: 0 511 1654 1694 1767
Size: 511 1143 40 73
Fragments arranged by size:
1143 511 73 40
SfoI GGC'GCC
Cuts at: 0 190 1767
Size: 190 1577
SmaI CCC'GGG
Cuts at: 0 46 1767
Size: 46 1721
SmlI C'TyrA_G
Cuts at: 0 7 37 145 811 1767
Size: 7 30 108 666 956
Fragments arranged by size:
956 666 108 30 7
SphI G_CATG'C
Cuts at: 0 1380 1767
Size: 1380 387
StyI C'CwwG_G
Cuts at: 0 686 764 1767
Size: 686 78 1003
Fragments arranged by size:
1003 686 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 403 1567 1767
Size: 403 1164 200
Fragments arranged by size:
1164 403 200
TatI w'GTAC_w
Cuts at: 0 923 1767
Size: 923 844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 285 317 760 792 983 1015 1540 1572
Size: 285 32 443 32 191 32 525 32
Cuts at: 1572 1767
Size: 195
Fragments arranged by size:
525 443 285 195 191 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1610 1767
Size: 1610 157
XhoI C'TCGA_G
Cuts at: 0 7 145 1767
Size: 7 138 1622
Fragments arranged by size:
1622 138 7
XmaI C'CCGG_G
Cuts at: 0 44 1767
Size: 44 1723
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApoI AvaI BanI
BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrGI
BssSI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII KasI MspA1I
NarI NcoI NspI PflMI PfoI PpuMI PspXI PstI
PvuII SacI SanDI SapI SfcI SfoI SmaI SmlI
SphI StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BmgBI BmrI
BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmI BsmBI
BspHI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EciI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XmnI ZraI