Restriction map for
pF1KB9909
(Linear) (Six-base) MAPSORT of: KIBB9909.seq Check: 9109 from: 1 to: 768
KIBB9909.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 237 456 507 768
Size: 237 219 51 261
Fragments arranged by size:
261 237 219 51
AvaI C'yCGr_G
Cuts at: 0 68 150 768
Size: 68 82 618
Fragments arranged by size:
618 82 68
BamHI G'GATC_C
Cuts at: 0 725 768
Size: 725 43
BanI G'GyrC_C
Cuts at: 0 172 445 481 768
Size: 172 273 36 287
Fragments arranged by size:
287 273 172 36
BanII G_rGCy'C
Cuts at: 0 261 284 518 768
Size: 261 23 234 250
Fragments arranged by size:
261 250 234 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 579 613 768
Size: 579 34 155
Fragments arranged by size:
579 155 34
BlpI GC'TnA_GC
Cuts at: 0 55 370 768
Size: 55 315 398
Fragments arranged by size:
398 315 55
Bme1580I G_kGCm'C
Cuts at: 0 95 440 486 768
Size: 95 345 46 282
Fragments arranged by size:
345 282 95 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 444 768
Size: 444 324
Bpu10I CC'TnA_GC
Cuts at: 0 308 768
Size: 308 460
BsaAI yAC'GTr
Cuts at: 0 717 768
Size: 717 51
BsaHI Gr'CG_yC
Cuts at: 0 31 768
Size: 31 737
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 768
Size: 112 30 626
Fragments arranged by size:
626 112 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 768
Size: 349 419
BseYI C'CCAG_C
Cuts at: 0 16 187 230 287 378 467 495 768
Size: 16 171 43 57 91 89 28 273
Fragments arranged by size:
273 171 91 89 57 43 28 16
BsiEI CG_ry'CG
Cuts at: 0 206 768
Size: 206 562
BsiHKAI G_wGCw'C
Cuts at: 0 254 518 768
Size: 254 264 250
Fragments arranged by size:
264 254 250
Bsp1286I G_dGCh'C
Cuts at: 0 95 254 261 284 440 486 518 768
Size: 95 159 7 23 156 46 32 250
Fragments arranged by size:
250 159 156 95 46 32 23 7
BsrBI CCG'CTC
Cuts at: 0 59 768
Size: 59 709
BsrFI r'CCGG_y
Cuts at: 0 8 545 586 768
Size: 8 537 41 182
Fragments arranged by size:
537 182 41 8
BssSI C'ACGA_G
Cuts at: 0 94 768
Size: 94 674
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 452 768
Size: 216 236 316
Fragments arranged by size:
316 236 216
BstEII G'GTnAC_C
Cuts at: 0 531 768
Size: 531 237
BstYI r'GATC_y
Cuts at: 0 725 768
Size: 725 43
BtgI C'CryG_G
Cuts at: 0 441 461 527 768
Size: 441 20 66 241
Fragments arranged by size:
441 241 66 20
BtsI GCAGTG_nn'
Cuts at: 0 210 768
Size: 210 558
DraIII CAC_nnn'GTG
Cuts at: 0 267 452 768
Size: 267 185 316
Fragments arranged by size:
316 267 185
EaeI y'GGCC_r
Cuts at: 0 203 458 547 768
Size: 203 255 89 221
Fragments arranged by size:
255 221 203 89
EagI C'GGCC_G
Cuts at: 0 203 768
Size: 203 565
EcoICRI GAG'CTC
Cuts at: 0 516 768
Size: 516 252
EcoO109I rG'GnC_Cy
Cuts at: 0 504 768
Size: 504 264
HaeII r_GCGC'y
Cuts at: 0 237 768
Size: 237 531
MscI TGG'CCA
Cuts at: 0 460 768
Size: 460 308
MspA1I CmG'CkG
Cuts at: 0 369 414 768
Size: 369 45 354
Fragments arranged by size:
369 354 45
NaeI GCC'GGC
Cuts at: 0 547 588 768
Size: 547 41 180
Fragments arranged by size:
547 180 41
NcoI C'CATG_G
Cuts at: 0 461 768
Size: 461 307
NgoMIV G'CCGG_C
Cuts at: 0 545 586 768
Size: 545 41 182
Fragments arranged by size:
545 182 41
PflMI CCAn_nnn'nTGG
Cuts at: 0 237 456 467 768
Size: 237 219 11 301
Fragments arranged by size:
301 237 219 11
PpuMI rG'GwC_Cy
Cuts at: 0 504 768
Size: 504 264
PstI C_TGCA'G
Cuts at: 0 137 512 768
Size: 137 375 256
Fragments arranged by size:
375 256 137
PvuII CAG'CTG
Cuts at: 0 369 414 768
Size: 369 45 354
Fragments arranged by size:
369 354 45
SacI G_AGCT'C
Cuts at: 0 518 768
Size: 518 250
SbfI CC_TGCA'GG
Cuts at: 0 512 768
Size: 512 256
SfcI C'TryA_G
Cuts at: 0 133 508 768
Size: 133 375 260
Fragments arranged by size:
375 260 133
SmaI CCC'GGG
Cuts at: 0 152 768
Size: 152 616
StyI C'CwwG_G
Cuts at: 0 461 639 755 768
Size: 461 178 116 13
Fragments arranged by size:
461 178 116 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 301 463 469 768
Size: 301 162 6 299
Fragments arranged by size:
301 299 162 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 24 768
Size: 24 744
XmaI C'CCGG_G
Cuts at: 0 150 768
Size: 150 618
Enzymes that do cut:
AlwNI AvaI BamHI BanI BanII BcgI BlpI Bme1580I
BmrI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstAPI BstEII BstYI
BtgI BtsI DraIII EaeI EagI EcoICRI EcoO109I HaeII
MscI MspA1I NaeI NcoI NgoMIV PflMI PpuMI PstI
PvuII SacI SbfI SfcI SmaI StyI TaqII XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BplI BpmI BpuEI BsaI BsaBI BsaWI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI NarI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmnI ZraI