Restriction map for
pF1KB9910
(Linear) (Six-base) MAPSORT of: KIBB9910.seq Check: 5350 from: 1 to: 1770
KIBB9910.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1387 1514 1770
Size: 1387 127 256
Fragments arranged by size:
1387 256 127
AatII G_ACGT'C
Cuts at: 0 1619 1770
Size: 1619 151
Acc65I G'GTAC_C
Cuts at: 0 548 1690 1770
Size: 548 1142 80
Fragments arranged by size:
1142 548 80
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 431 509 1168 1303 1770
Size: 217 214 78 659 135 467
Fragments arranged by size:
659 467 217 214 135 78
AfeI AGC'GCT
Cuts at: 0 799 1770
Size: 799 971
AflIII A'CryG_T
Cuts at: 0 707 1770
Size: 707 1063
AleI CACnn'nnGTG
Cuts at: 0 265 568 1606 1770
Size: 265 303 1038 164
Fragments arranged by size:
1038 303 265 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 818 1770
Size: 818 952
AlwNI CAG_nnn'CTG
Cuts at: 0 875 1379 1392 1407 1770
Size: 875 504 13 15 363
Fragments arranged by size:
875 504 363 15 13
ApoI r'AATT_y
Cuts at: 0 148 1770
Size: 148 1622
AvaI C'yCGr_G
Cuts at: 0 158 603 886 1770
Size: 158 445 283 884
Fragments arranged by size:
884 445 283 158
BanI G'GyrC_C
Cuts at: 0 19 548 1690 1770
Size: 19 529 1142 80
Fragments arranged by size:
1142 529 80 19
BanII G_rGCy'C
Cuts at: 0 766 912 1038 1770
Size: 766 146 126 732
Fragments arranged by size:
766 732 146 126
BbeI G_GCGC'C
Cuts at: 0 23 1770
Size: 23 1747
BbsI GAAGACnn'nnnn_
Cuts at: 0 1486 1770
Size: 1486 284
BclI T'GATC_A
Cuts at: 0 368 1770
Size: 368 1402
BglI GCCn_nnn'nGGC
Cuts at: 0 880 1770
Size: 880 890
Bme1580I G_kGCm'C
Cuts at: 0 853 1770
Size: 853 917
BmgBI CAC'GTC
Cuts at: 0 1455 1770
Size: 1455 315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 209 1579 1690 1770
Size: 122 87 1370 111 80
Fragments arranged by size:
1370 122 111 87 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1489 1770
Size: 1489 281
BsaI GGTCTCn'nnnn_
Cuts at: 0 227 415 1096 1770
Size: 227 188 681 674
Fragments arranged by size:
681 674 227 188
BsaAI yAC'GTr
Cuts at: 0 461 710 1770
Size: 461 249 1060
Fragments arranged by size:
1060 461 249
BsaHI Gr'CG_yC
Cuts at: 0 20 824 1616 1770
Size: 20 804 792 154
Fragments arranged by size:
804 792 154 20
BsaWI w'CCGG_w
Cuts at: 0 614 969 1770
Size: 614 355 801
Fragments arranged by size:
801 614 355
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 694 724 1643 1673 1770
Size: 694 30 919 30 97
Fragments arranged by size:
919 694 97 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 659 766 956 1616 1770
Size: 659 107 190 660 154
Fragments arranged by size:
660 659 190 154 107
BseYI C'CCAG_C
Cuts at: 0 426 1445 1770
Size: 426 1019 325
Fragments arranged by size:
1019 426 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 814 1378 1770
Size: 346 468 564 392
Fragments arranged by size:
564 468 392 346
BsiEI CG_ry'CG
Cuts at: 0 56 1770
Size: 56 1714
BsiHKAI G_wGCw'C
Cuts at: 0 614 707 1237 1442 1770
Size: 614 93 530 205 328
Fragments arranged by size:
614 530 328 205 93
BsiWI C'GTAC_G
Cuts at: 0 1082 1770
Size: 1082 688
BsmI GAATG_Cn'
Cuts at: 0 142 1770
Size: 142 1628
Bsp1286I G_dGCh'C
Cuts at: 0 614 707 766 853 912 1038 1237 1442
Size: 614 93 59 87 59 126 199 205
Cuts at: 1442 1770
Size: 328
Fragments arranged by size:
614 328 205 199 126 93 87 59
59
BspEI T'CCGG_A
Cuts at: 0 614 1770
Size: 614 1156
BspHI T'CATG_A
Cuts at: 0 819 1770
Size: 819 951
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 318 1387 1514 1678 1770
Size: 293 25 1069 127 164 92
Fragments arranged by size:
1069 293 164 127 92 25
BsrDI GCAATG_nn'
Cuts at: 0 1277 1619 1770
Size: 1277 342 151
Fragments arranged by size:
1277 342 151
BtgI C'CryG_G
Cuts at: 0 696 1770
Size: 696 1074
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1668 1770
Size: 1668 102
BtsI GCAGTG_nn'
Cuts at: 0 1483 1740 1770
Size: 1483 257 30
Fragments arranged by size:
1483 257 30
DraI TTT'AAA
Cuts at: 0 1026 1770
Size: 1026 744
DrdI GACnn_nn'nnGTC
Cuts at: 0 931 1770
Size: 931 839
EaeI y'GGCC_r
Cuts at: 0 53 81 290 1770
Size: 53 28 209 1480
Fragments arranged by size:
1480 209 53 28
EagI C'GGCC_G
Cuts at: 0 53 1770
Size: 53 1717
EarI CTCTTCn'nnn_
Cuts at: 0 193 352 1770
Size: 193 159 1418
Fragments arranged by size:
1418 193 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 209 217 431 509 1168 1303 1579
Size: 122 87 8 214 78 659 135 276
Cuts at: 1579 1690 1770
Size: 111 80
Fragments arranged by size:
659 276 214 135 122 111 87 80
78 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 867 1770
Size: 867 903
EcoO109I rG'GnC_Cy
Cuts at: 0 468 872 1208 1404 1770
Size: 468 404 336 196 366
Fragments arranged by size:
468 404 366 336 196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 673 1770
Size: 673 1097
FspI TGC'GCA
Cuts at: 0 775 1770
Size: 775 995
HaeII r_GCGC'y
Cuts at: 0 23 801 1770
Size: 23 778 969
Fragments arranged by size:
969 778 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1115 1135 1147 1167 1770
Size: 1115 20 12 20 603
Fragments arranged by size:
1115 603 20 20 12
HincII GTy'rAC
Cuts at: 0 1154 1770
Size: 1154 616
HindIII A'AGCT_T
Cuts at: 0 529 1423 1770
Size: 529 894 347
Fragments arranged by size:
894 529 347
KasI G'GCGC_C
Cuts at: 0 19 1770
Size: 19 1751
KpnI G_GTAC'C
Cuts at: 0 552 1694 1770
Size: 552 1142 76
Fragments arranged by size:
1142 552 76
MslI CAynn'nnrTG
Cuts at: 0 265 568 955 1606 1762 1770
Size: 265 303 387 651 156 8
Fragments arranged by size:
651 387 303 265 156 8
MspA1I CmG'CkG
Cuts at: 0 265 769 856 1326 1413 1449 1770
Size: 265 504 87 470 87 36 321
Fragments arranged by size:
504 470 321 265 87 87 36
NarI GG'CG_CC
Cuts at: 0 20 1770
Size: 20 1750
NspI r_CATG'y
Cuts at: 0 584 1770
Size: 584 1186
PasI CC'CwG_GG
Cuts at: 0 869 1770
Size: 869 901
PflMI CCAn_nnn'nTGG
Cuts at: 0 1077 1770
Size: 1077 693
PfoI T'CCnGG_A
Cuts at: 0 998 1667 1770
Size: 998 669 103
Fragments arranged by size:
998 669 103
PmlI CAC'GTG
Cuts at: 0 461 1770
Size: 461 1309
PpuMI rG'GwC_Cy
Cuts at: 0 468 1404 1770
Size: 468 936 366
Fragments arranged by size:
936 468 366
PshAI GACnn'nnGTC
Cuts at: 0 827 1770
Size: 827 943
PsiI TTA'TAA
Cuts at: 0 479 1770
Size: 479 1291
PstI C_TGCA'G
Cuts at: 0 1331 1412 1770
Size: 1331 81 358
Fragments arranged by size:
1331 358 81
PvuII CAG'CTG
Cuts at: 0 856 1326 1413 1449 1770
Size: 856 470 87 36 321
Fragments arranged by size:
856 470 321 87 36
SfcI C'TryA_G
Cuts at: 0 1327 1408 1770
Size: 1327 81 362
Fragments arranged by size:
1327 362 81
SfoI GGC'GCC
Cuts at: 0 21 1770
Size: 21 1749
SmaI CCC'GGG
Cuts at: 0 160 1770
Size: 160 1610
SmlI C'TyrA_G
Cuts at: 0 603 1468 1770
Size: 603 865 302
Fragments arranged by size:
865 603 302
SpeI A'CTAG_T
Cuts at: 0 1218 1770
Size: 1218 552
SphI G_CATG'C
Cuts at: 0 584 1770
Size: 584 1186
StyI C'CwwG_G
Cuts at: 0 845 1312 1770
Size: 845 467 458
Fragments arranged by size:
845 467 458
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1264 1278 1770
Size: 1264 14 492
Fragments arranged by size:
1264 492 14
TatI w'GTAC_w
Cuts at: 0 1524 1770
Size: 1524 246
XhoI C'TCGA_G
Cuts at: 0 603 1770
Size: 603 1167
XmaI C'CCGG_G
Cuts at: 0 158 1770
Size: 158 1612
XmnI GAAnn'nnTTC
Cuts at: 0 1175 1770
Size: 1175 595
ZraI GAC'GTC
Cuts at: 0 1617 1770
Size: 1617 153
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AleI AlfI
AlwNI ApoI AvaI BanI BanII BbeI BbsI BclI
BglI Bme1580I BmgBI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrDI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MslI MspA1I NarI NspI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PstI PvuII SfcI SfoI
SmaI SmlI SpeI SphI StyI TaqII TatI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I
BsaBI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraIII EciI EcoICRI EcoRI EcoRV FseI FspAI HpaI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI