(Linear) (Six-base) MAPSORT of: KIBB9925.seq Check: 7723 from: 1 to: 1038 KIBB9925.orf With 182 enzymes: SgfI * April 24, 2009 13:09 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 463 1038 Size: 382 81 575 Fragments arranged by size: 575 382 81 AflIII A'CryG_T Cuts at: 0 908 1038 Size: 908 130 AlwNI CAG_nnn'CTG Cuts at: 0 778 1038 Size: 778 260 ApoI r'AATT_y Cuts at: 0 543 677 1038 Size: 543 134 361 Fragments arranged by size: 543 361 134 AvaI C'yCGr_G Cuts at: 0 11 755 1038 Size: 11 744 283 Fragments arranged by size: 744 283 11 BanII G_rGCy'C Cuts at: 0 848 1038 Size: 848 190 BbsI GAAGACnn'nnnn_ Cuts at: 0 574 1038 Size: 574 464 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 724 758 1038 Size: 724 34 280 Fragments arranged by size: 724 280 34 BclI T'GATC_A Cuts at: 0 423 1038 Size: 423 615 BglII A'GATC_T Cuts at: 0 178 881 1038 Size: 178 703 157 Fragments arranged by size: 703 178 157 Bme1580I G_kGCm'C Cuts at: 0 390 1038 Size: 390 648 BmrI ACTGGGnnnn_n' Cuts at: 0 125 1038 Size: 125 913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 862 1022 1038 Size: 862 160 16 Fragments arranged by size: 862 160 16 BsaI GGTCTCn'nnnn_ Cuts at: 0 490 1038 Size: 490 548 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 995 1025 1038 Size: 995 30 13 Fragments arranged by size: 995 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 94 125 912 1038 Size: 94 31 787 126 Fragments arranged by size: 787 126 94 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 205 572 776 1038 Size: 205 367 204 262 Fragments arranged by size: 367 262 205 204 BsiHKAI G_wGCw'C Cuts at: 0 848 1033 1038 Size: 848 185 5 Fragments arranged by size: 848 185 5 BsmI GAATG_Cn' Cuts at: 0 745 1038 Size: 745 293 Bsp1286I G_dGCh'C Cuts at: 0 390 848 1033 1038 Size: 390 458 185 5 Fragments arranged by size: 458 390 185 5 BspHI T'CATG_A Cuts at: 0 245 1038 Size: 245 793 BsrDI GCAATG_nn' Cuts at: 0 863 1038 Size: 863 175 BstAPI GCAn_nnn'nTGC Cuts at: 0 453 1038 Size: 453 585 BstYI r'GATC_y Cuts at: 0 178 881 1038 Size: 178 703 157 Fragments arranged by size: 703 178 157 BtsI GCAGTG_nn' Cuts at: 0 35 302 390 1038 Size: 35 267 88 648 Fragments arranged by size: 648 267 88 35 EarI CTCTTCn'nnn_ Cuts at: 0 954 1038 Size: 954 84 EcoICRI GAG'CTC Cuts at: 0 846 1038 Size: 846 192 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 463 862 1022 1038 Size: 382 81 399 160 16 Fragments arranged by size: 399 382 160 81 16 EcoRV GAT'ATC Cuts at: 0 306 1038 Size: 306 732 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 995 1027 1038 Size: 995 32 11 Fragments arranged by size: 995 32 11 HincII GTy'rAC Cuts at: 0 827 1038 Size: 827 211 MslI CAynn'nnrTG Cuts at: 0 800 913 1038 Size: 800 113 125 Fragments arranged by size: 800 125 113 NspI r_CATG'y Cuts at: 0 912 1038 Size: 912 126 PciI A'CATG_T Cuts at: 0 908 1038 Size: 908 130 PstI C_TGCA'G Cuts at: 0 30 327 385 458 1038 Size: 30 297 58 73 580 Fragments arranged by size: 580 297 73 58 30 SacI G_AGCT'C Cuts at: 0 848 1038 Size: 848 190 SfcI C'TryA_G Cuts at: 0 26 102 323 381 454 1038 Size: 26 76 221 58 73 584 Fragments arranged by size: 584 221 76 73 58 26 SmaI CCC'GGG Cuts at: 0 13 1038 Size: 13 1025 SmlI C'TyrA_G Cuts at: 0 5 1038 Size: 5 1033 SspI AAT'ATT Cuts at: 0 784 940 1038 Size: 784 156 98 Fragments arranged by size: 784 156 98 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 275 1038 Size: 275 763 TatI w'GTAC_w Cuts at: 0 98 1038 Size: 98 940 XmaI C'CCGG_G Cuts at: 0 11 1038 Size: 11 1027 XmnI GAAnn'nnTTC Cuts at: 0 376 1038 Size: 376 662 Enzymes that do cut: AcuI AflIII AlwNI ApoI AvaI BanII BbsI BcgI BclI BglII Bme1580I BmrI BpmI BsaI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstAPI BstYI BtsI EarI EcoICRI Eco57MI EcoRV Hin4I HincII MslI NspI PciI PstI SacI SfcI SmaI SmlI SspI TaqII TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI ZraI