Restriction map for
pF1KB9928
(Linear) (Six-base) MAPSORT of: KIBB9928.seq Check: 2983 from: 1 to: 1878
KIBB9928.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 805 979 1384 1765 1878
Size: 59 746 174 405 381 113
Fragments arranged by size:
746 405 381 174 113 59
AfeI AGC'GCT
Cuts at: 0 516 1878
Size: 516 1362
AflIII A'CryG_T
Cuts at: 0 1460 1878
Size: 1460 418
ApoI r'AATT_y
Cuts at: 0 423 801 1878
Size: 423 378 1077
Fragments arranged by size:
1077 423 378
AvaI C'yCGr_G
Cuts at: 0 1299 1878
Size: 1299 579
BanI G'GyrC_C
Cuts at: 0 1466 1878
Size: 1466 412
BanII G_rGCy'C
Cuts at: 0 1697 1878
Size: 1697 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 1878
Size: 287 1591
BbvCI CC'TCA_GC
Cuts at: 0 1378 1441 1878
Size: 1378 63 437
Fragments arranged by size:
1378 437 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 749 783 1878
Size: 749 34 1095
Fragments arranged by size:
1095 749 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 349 1878
Size: 349 1529
BglI GCCn_nnn'nGGC
Cuts at: 0 1070 1878
Size: 1070 808
BmgBI CAC'GTC
Cuts at: 0 1461 1878
Size: 1461 417
BmrI ACTGGGnnnn_n'
Cuts at: 0 1660 1737 1878
Size: 1660 77 141
Fragments arranged by size:
1660 141 77
BmtI G_CTAG'C
Cuts at: 0 515 1878
Size: 515 1363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 943 1235 1668 1780 1816 1878
Size: 685 258 292 433 112 36 62
Fragments arranged by size:
685 433 292 258 112 62 36
Bpu10I CC'TnA_GC
Cuts at: 0 809 1378 1441 1878
Size: 809 569 63 437
Fragments arranged by size:
809 569 437 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 1878
Size: 1333 545
BsaI GGTCTCn'nnnn_
Cuts at: 0 1150 1234 1772 1878
Size: 1150 84 538 106
Fragments arranged by size:
1150 538 106 84
BsaAI yAC'GTr
Cuts at: 0 302 1878
Size: 302 1576
BsaWI w'CCGG_w
Cuts at: 0 321 459 1878
Size: 321 138 1419
Fragments arranged by size:
1419 321 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 664 694 916 946 1214 1244 1429 1459
Size: 664 30 222 30 268 30 185 30
Cuts at: 1459 1878
Size: 419
Fragments arranged by size:
664 419 268 222 185 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 138 1222 1671 1878
Size: 138 1084 449 207
Fragments arranged by size:
1084 449 207 138
BseYI C'CCAG_C
Cuts at: 0 683 1878
Size: 683 1195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1017 1747 1878
Size: 1017 730 131
Fragments arranged by size:
1017 730 131
BsiEI CG_ry'CG
Cuts at: 0 1074 1878
Size: 1074 804
BsiHKAI G_wGCw'C
Cuts at: 0 7 1878
Size: 7 1871
BsmI GAATG_Cn'
Cuts at: 0 55 1878
Size: 55 1823
Bsp1286I G_dGCh'C
Cuts at: 0 7 1697 1878
Size: 7 1690 181
Fragments arranged by size:
1690 181 7
BspEI T'CCGG_A
Cuts at: 0 321 1878
Size: 321 1557
BsrDI GCAATG_nn'
Cuts at: 0 438 1565 1878
Size: 438 1127 313
Fragments arranged by size:
1127 438 313
BstAPI GCAn_nnn'nTGC
Cuts at: 0 251 1185 1310 1878
Size: 251 934 125 568
Fragments arranged by size:
934 568 251 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 61 856 1455 1878
Size: 61 795 599 423
Fragments arranged by size:
795 599 423 61
BstYI r'GATC_y
Cuts at: 0 1150 1847 1878
Size: 1150 697 31
Fragments arranged by size:
1150 697 31
BtgI C'CryG_G
Cuts at: 0 60 1878
Size: 60 1818
BtsI GCAGTG_nn'
Cuts at: 0 168 450 1878
Size: 168 282 1428
Fragments arranged by size:
1428 282 168
EaeI y'GGCC_r
Cuts at: 0 1071 1812 1878
Size: 1071 741 66
Fragments arranged by size:
1071 741 66
EagI C'GGCC_G
Cuts at: 0 1071 1878
Size: 1071 807
EarI CTCTTCn'nnn_
Cuts at: 0 962 1878
Size: 962 916
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1008 1878
Size: 1008 870
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 685 805 943 979 1235 1384 1668
Size: 59 626 120 138 36 256 149 284
Cuts at: 1668 1765 1780 1816 1878
Size: 97 15 36 62
Fragments arranged by size:
626 284 256 149 138 120 97 62
59 36 36 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1705 1878
Size: 1705 173
EcoO109I rG'GnC_Cy
Cuts at: 0 1353 1878
Size: 1353 525
EcoRI G'AATT_C
Cuts at: 0 423 1878
Size: 423 1455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1158 1878
Size: 1158 720
HaeII r_GCGC'y
Cuts at: 0 159 518 1878
Size: 159 359 1360
Fragments arranged by size:
1360 359 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 114 146 802 834 888 920 1214 1246
Size: 114 32 656 32 54 32 294 32
Cuts at: 1246 1429 1461 1878
Size: 183 32 417
Fragments arranged by size:
656 417 294 183 114 54 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1101 1878
Size: 1101 777
MscI TGG'CCA
Cuts at: 0 1814 1878
Size: 1814 64
MslI CAynn'nnrTG
Cuts at: 0 59 443 1453 1666 1878
Size: 59 384 1010 213 212
Fragments arranged by size:
1010 384 213 212 59
MspA1I CmG'CkG
Cuts at: 0 852 1878
Size: 852 1026
NcoI C'CATG_G
Cuts at: 0 60 1878
Size: 60 1818
NheI G'CTAG_C
Cuts at: 0 511 1878
Size: 511 1367
NspI r_CATG'y
Cuts at: 0 494 1878
Size: 494 1384
PfoI T'CCnGG_A
Cuts at: 0 104 503 1037 1878
Size: 104 399 534 841
Fragments arranged by size:
841 534 399 104
PmlI CAC'GTG
Cuts at: 0 302 1878
Size: 302 1576
PpuMI rG'GwC_Cy
Cuts at: 0 1353 1878
Size: 1353 525
PsiI TTA'TAA
Cuts at: 0 545 1878
Size: 545 1333
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 655 687 1878
Size: 655 32 1191
Fragments arranged by size:
1191 655 32
PstI C_TGCA'G
Cuts at: 0 1190 1878
Size: 1190 688
PvuII CAG'CTG
Cuts at: 0 852 1878
Size: 852 1026
SapI GCTCTTCn'nnn_
Cuts at: 0 962 1878
Size: 962 916
ScaI AGT'ACT
Cuts at: 0 1318 1878
Size: 1318 560
SfcI C'TryA_G
Cuts at: 0 1186 1878
Size: 1186 692
SmlI C'TyrA_G
Cuts at: 0 1348 1878
Size: 1348 530
SphI G_CATG'C
Cuts at: 0 494 1878
Size: 494 1384
StuI AGG'CCT
Cuts at: 0 1255 1819 1861 1878
Size: 1255 564 42 17
Fragments arranged by size:
1255 564 42 17
StyI C'CwwG_G
Cuts at: 0 60 1265 1286 1878
Size: 60 1205 21 592
Fragments arranged by size:
1205 592 60 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1852 1878
Size: 1852 26
TatI w'GTAC_w
Cuts at: 0 1316 1878
Size: 1316 562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 916 948 1450 1482 1777 1809 1878
Size: 916 32 502 32 295 32 69
Fragments arranged by size:
916 502 295 69 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1226 1878
Size: 1226 652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 181 918 1878
Size: 181 737 960
Fragments arranged by size:
960 737 181
Enzymes that do cut:
AcuI AfeI AflIII ApoI AvaI BanI BanII BbsI
BbvCI BcgI BciVI BglI BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BsrDI BstAPI
BstXI BstYI BtgI BtsI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
MscI MslI MspA1I NcoI NheI NspI PfoI PmlI
PpuMI PsiI PsrI PstI PvuII SapI ScaI SfcI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BclI BfrBI BglII
BlpI Bme1580I BplI BsaBI BsaHI BsiWI BsmBI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PpiI PshAI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI XbaI XhoI
XmaI XmnI ZraI