Restriction map for
pF1KB9933
(Linear) (Six-base) MAPSORT of: KIBB9933.seq Check: 3662 from: 1 to: 1467
KIBB9933.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AccI GT'mk_AC
Cuts at: 0 845 1467
Size: 845 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 699 1231 1467
Size: 699 532 236
Fragments arranged by size:
699 532 236
AflIII A'CryG_T
Cuts at: 0 923 1229 1467
Size: 923 306 238
Fragments arranged by size:
923 306 238
AleI CACnn'nnGTG
Cuts at: 0 628 838 1467
Size: 628 210 629
Fragments arranged by size:
629 628 210
AlwNI CAG_nnn'CTG
Cuts at: 0 789 1377 1467
Size: 789 588 90
Fragments arranged by size:
789 588 90
ApaI G_GGCC'C
Cuts at: 0 663 810 1109 1284 1386 1467
Size: 663 147 299 175 102 81
Fragments arranged by size:
663 299 175 147 102 81
ApoI r'AATT_y
Cuts at: 0 31 1467
Size: 31 1436
AvaI C'yCGr_G
Cuts at: 0 763 795 1108 1260 1467
Size: 763 32 313 152 207
Fragments arranged by size:
763 313 207 152 32
AvrII C'CTAG_G
Cuts at: 0 1275 1467
Size: 1275 192
BanI G'GyrC_C
Cuts at: 0 172 419 622 1467
Size: 172 247 203 845
Fragments arranged by size:
845 247 203 172
BanII G_rGCy'C
Cuts at: 0 663 810 1040 1109 1267 1284 1386 1467
Size: 663 147 230 69 158 17 102 81
Fragments arranged by size:
663 230 158 147 102 81 69 17
BbvCI CC'TCA_GC
Cuts at: 0 1127 1467
Size: 1127 340
BglI GCCn_nnn'nGGC
Cuts at: 0 1123 1345 1390 1467
Size: 1123 222 45 77
Fragments arranged by size:
1123 222 77 45
BlpI GC'TnA_GC
Cuts at: 0 576 1467
Size: 576 891
Bme1580I G_kGCm'C
Cuts at: 0 242 392 490 625 663 810 1109 1193
Size: 242 150 98 135 38 147 299 84
Cuts at: 1193 1284 1386 1467
Size: 91 102 81
Fragments arranged by size:
299 242 150 147 135 102 98 91
84 81 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 852 1000 1141 1378 1450 1467
Size: 403 449 148 141 237 72 17
Fragments arranged by size:
449 403 237 148 141 72 17
Bpu10I CC'TnA_GC
Cuts at: 0 193 1127 1467
Size: 193 934 340
Fragments arranged by size:
934 340 193
BsaAI yAC'GTr
Cuts at: 0 1188 1467
Size: 1188 279
BsaBI GATnn'nnATC
Cuts at: 0 1165 1467
Size: 1165 302
BsaWI w'CCGG_w
Cuts at: 0 875 1467
Size: 875 592
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 487 517 1467
Size: 487 30 950
Fragments arranged by size:
950 487 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 511 910 1144 1257 1375 1447 1467
Size: 511 399 234 113 118 72 20
Fragments arranged by size:
511 399 234 118 113 72 20
BseYI C'CCAG_C
Cuts at: 0 1192 1318 1348 1440 1467
Size: 1192 126 30 92 27
Fragments arranged by size:
1192 126 92 30 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 470 505 1467
Size: 297 173 35 962
Fragments arranged by size:
962 297 173 35
BsiEI CG_ry'CG
Cuts at: 0 1416 1467
Size: 1416 51
BsiHKAI G_wGCw'C
Cuts at: 0 188 1467
Size: 188 1279
Bsp1286I G_dGCh'C
Cuts at: 0 188 242 392 490 625 663 810 1040
Size: 188 54 150 98 135 38 147 230
Cuts at: 1040 1109 1193 1267 1284 1386 1467
Size: 69 84 74 17 102 81
Fragments arranged by size:
230 188 150 147 135 102 98 84
81 74 69 54 38 17
BspHI T'CATG_A
Cuts at: 0 651 1046 1467
Size: 651 395 421
Fragments arranged by size:
651 421 395
BsrBI CCG'CTC
Cuts at: 0 639 732 1142 1467
Size: 639 93 410 325
Fragments arranged by size:
639 410 325 93
BsrFI r'CCGG_y
Cuts at: 0 54 369 1220 1244 1467
Size: 54 315 851 24 223
Fragments arranged by size:
851 315 223 54 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 119 1377 1467
Size: 119 1258 90
Fragments arranged by size:
1258 119 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 402 684 1467
Size: 141 261 282 783
Fragments arranged by size:
783 282 261 141
BstYI r'GATC_y
Cuts at: 0 332 1467
Size: 332 1135
Bsu36I CC'TnA_GG
Cuts at: 0 1387 1467
Size: 1387 80
BtgI C'CryG_G
Cuts at: 0 425 773 1253 1467
Size: 425 348 480 214
Fragments arranged by size:
480 425 348 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 396 1467
Size: 396 1071
BtsI GCAGTG_nn'
Cuts at: 0 1171 1298 1467
Size: 1171 127 169
Fragments arranged by size:
1171 169 127
DrdI GACnn_nn'nnGTC
Cuts at: 0 316 1467
Size: 316 1151
EaeI y'GGCC_r
Cuts at: 0 1331 1467
Size: 1331 136
EarI CTCTTCn'nnn_
Cuts at: 0 1078 1467
Size: 1078 389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 699 852 1000 1141 1231 1378 1450
Size: 403 296 153 148 141 90 147 72
Cuts at: 1450 1467
Size: 17
Fragments arranged by size:
403 296 153 148 147 141 90 72
17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1075 1467
Size: 1075 392
EcoO109I rG'GnC_Cy
Cuts at: 0 247 659 750 806 807 975 1206 1280
Size: 247 412 91 56 1 168 231 74
Cuts at: 1280 1281 1383 1467
Size: 1 102 84
Fragments arranged by size:
412 247 231 168 102 91 84 74
56 1 1
FspI TGC'GCA
Cuts at: 0 601 1467
Size: 601 866
FspAI rTGC'GCAy
Cuts at: 0 601 1467
Size: 601 866
HaeII r_GCGC'y
Cuts at: 0 745 1396 1467
Size: 745 651 71
Fragments arranged by size:
745 651 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 392 424 1467
Size: 392 32 1043
Fragments arranged by size:
1043 392 32
HincII GTy'rAC
Cuts at: 0 129 1467
Size: 129 1338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1467
Size: 1046 421
MscI TGG'CCA
Cuts at: 0 1333 1467
Size: 1333 134
MslI CAynn'nnrTG
Cuts at: 0 88 319 628 838 1045 1051 1467
Size: 88 231 309 210 207 6 416
Fragments arranged by size:
416 309 231 210 207 88 6
MspA1I CmG'CkG
Cuts at: 0 299 360 493 548 738 948 1203 1322
Size: 299 61 133 55 190 210 255 119
Cuts at: 1322 1440 1467
Size: 118 27
Fragments arranged by size:
299 255 210 190 133 119 118 61
55 27
NaeI GCC'GGC
Cuts at: 0 1222 1246 1467
Size: 1222 24 221
Fragments arranged by size:
1222 221 24
NcoI C'CATG_G
Cuts at: 0 773 1253 1467
Size: 773 480 214
Fragments arranged by size:
773 480 214
NgoMIV G'CCGG_C
Cuts at: 0 1220 1244 1467
Size: 1220 24 223
Fragments arranged by size:
1220 223 24
NspI r_CATG'y
Cuts at: 0 43 584 966 1467
Size: 43 541 382 501
Fragments arranged by size:
541 501 382 43
PfoI T'CCnGG_A
Cuts at: 0 254 1076 1467
Size: 254 822 391
Fragments arranged by size:
822 391 254
PpuMI rG'GwC_Cy
Cuts at: 0 247 750 1206 1467
Size: 247 503 456 261
Fragments arranged by size:
503 456 261 247
PshAI GACnn'nnGTC
Cuts at: 0 269 614 1467
Size: 269 345 853
Fragments arranged by size:
853 345 269
PspOMI G'GGCC_C
Cuts at: 0 659 806 1105 1280 1382 1467
Size: 659 147 299 175 102 85
Fragments arranged by size:
659 299 175 147 102 85
PspXI vC'TCGA_Gb
Cuts at: 0 763 795 1467
Size: 763 32 672
Fragments arranged by size:
763 672 32
PstI C_TGCA'G
Cuts at: 0 785 1467
Size: 785 682
PvuII CAG'CTG
Cuts at: 0 548 738 1440 1467
Size: 548 190 702 27
Fragments arranged by size:
702 548 190 27
SanDI GG'GwC_CC
Cuts at: 0 750 1467
Size: 750 717
SbfI CC_TGCA'GG
Cuts at: 0 785 1467
Size: 785 682
SfcI C'TryA_G
Cuts at: 0 781 1467
Size: 781 686
SmaI CCC'GGG
Cuts at: 0 1262 1467
Size: 1262 205
SmlI C'TyrA_G
Cuts at: 0 763 795 1467
Size: 763 32 672
Fragments arranged by size:
763 672 32
SphI G_CATG'C
Cuts at: 0 584 966 1467
Size: 584 382 501
Fragments arranged by size:
584 501 382
StuI AGG'CCT
Cuts at: 0 220 1183 1467
Size: 220 963 284
Fragments arranged by size:
963 284 220
StyI C'CwwG_G
Cuts at: 0 773 1253 1275 1467
Size: 773 480 22 192
Fragments arranged by size:
773 480 192 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1125 1467
Size: 1125 342
Tth111I GACn'n_nGTC
Cuts at: 0 286 1467
Size: 286 1181
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 975 1326 1467
Size: 975 351 141
Fragments arranged by size:
975 351 141
XhoI C'TCGA_G
Cuts at: 0 763 795 1467
Size: 763 32 672
Fragments arranged by size:
763 672 32
XmaI C'CCGG_G
Cuts at: 0 1260 1467
Size: 1260 207
XmnI GAAnn'nnTTC
Cuts at: 0 925 1467
Size: 925 542
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaI ApoI AvaI
AvrII BanI BanII BbvCI BglI BlpI Bme1580I BpmI
Bpu10I BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SanDI
SbfI SfcI SmaI SmlI SphI StuI StyI TaqII
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI
BbeI BbsI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BmtI BplI BpuEI BsaI BsaHI BsiWI BsmI
BsmBI BspEI BspMI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I ClaI CspCI DraI DraIII EagI EciI
EcoICRI EcoRI EcoRV FalI FseI HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PsiI PsrI PvuI RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TatI TstI XbaI ZraI