Restriction map for
pF1KB9936
(Linear) (Six-base) MAPSORT of: KIBB9936.seq Check: 5515 from: 1 to: 1902
KIBB9936.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AatII G_ACGT'C
Cuts at: 0 1760 1902
Size: 1760 142
Acc65I G'GTAC_C
Cuts at: 0 1248 1902
Size: 1248 654
AflIII A'CryG_T
Cuts at: 0 1457 1902
Size: 1457 445
AhdI GACnn_n'nnGTC
Cuts at: 0 1029 1902
Size: 1029 873
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1511 1902
Size: 1511 391
AlwNI CAG_nnn'CTG
Cuts at: 0 150 1047 1647 1902
Size: 150 897 600 255
Fragments arranged by size:
897 600 255 150
ApaI G_GGCC'C
Cuts at: 0 163 1902
Size: 163 1739
ApaLI G'TGCA_C
Cuts at: 0 523 1902
Size: 523 1379
ApoI r'AATT_y
Cuts at: 0 25 1902
Size: 25 1877
AvaI C'yCGr_G
Cuts at: 0 258 790 1566 1902
Size: 258 532 776 336
Fragments arranged by size:
776 532 336 258
BanI G'GyrC_C
Cuts at: 0 220 287 400 874 1161 1248 1320 1902
Size: 220 67 113 474 287 87 72 582
Fragments arranged by size:
582 474 287 220 113 87 72 67
BanII G_rGCy'C
Cuts at: 0 163 800 1078 1417 1573 1902
Size: 163 637 278 339 156 329
Fragments arranged by size:
637 339 329 278 163 156
BbeI G_GCGC'C
Cuts at: 0 878 1165 1324 1902
Size: 878 287 159 578
Fragments arranged by size:
878 578 287 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 628 1902
Size: 628 1274
BciVI GTATCCnnnnn_n'
Cuts at: 0 1645 1902
Size: 1645 257
BglI GCCn_nnn'nGGC
Cuts at: 0 332 398 926 1489 1613 1667 1902
Size: 332 66 528 563 124 54 235
Fragments arranged by size:
563 528 332 235 124 66 54
Bme1580I G_kGCm'C
Cuts at: 0 163 527 1902
Size: 163 364 1375
Fragments arranged by size:
1375 364 163
BmgBI CAC'GTC
Cuts at: 0 522 1902
Size: 522 1380
BmrI ACTGGGnnnn_n'
Cuts at: 0 138 166 1260 1657 1902
Size: 138 28 1094 397 245
Fragments arranged by size:
1094 397 245 138 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 118 456 807 1057 1533 1534 1902
Size: 112 6 338 351 250 476 1 368
Fragments arranged by size:
476 368 351 338 250 112 6 1
Bpu10I CC'TnA_GC
Cuts at: 0 103 946 1902
Size: 103 843 956
Fragments arranged by size:
956 843 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1783 1902
Size: 1783 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 562 1902
Size: 562 1340
BsaBI GATnn'nnATC
Cuts at: 0 1303 1309 1902
Size: 1303 6 593
Fragments arranged by size:
1303 593 6
BsaHI Gr'CG_yC
Cuts at: 0 481 875 929 963 1025 1162 1321 1757
Size: 481 394 54 34 62 137 159 436
Cuts at: 1757 1902
Size: 145
Fragments arranged by size:
481 436 394 159 145 137 62 54
34
BsaWI w'CCGG_w
Cuts at: 0 1886 1902
Size: 1886 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 748 778 1902
Size: 748 30 1124
Fragments arranged by size:
1124 748 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 100 808 894 1106 1902
Size: 82 18 708 86 212 796
Fragments arranged by size:
796 708 212 86 82 18
BseYI C'CCAG_C
Cuts at: 0 163 921 1079 1100 1130 1198 1711 1866
Size: 163 758 158 21 30 68 513 155
Cuts at: 1866 1902
Size: 36
Fragments arranged by size:
758 513 163 158 155 68 36 30
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 448 794 1813 1902
Size: 31 417 346 1019 89
Fragments arranged by size:
1019 417 346 89 31
BsiHKAI G_wGCw'C
Cuts at: 0 475 527 895 952 1417 1902
Size: 475 52 368 57 465 485
Fragments arranged by size:
485 475 465 368 57 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 64 1902
Size: 64 1838
Bsp1286I G_dGCh'C
Cuts at: 0 163 475 527 800 895 952 1078 1417
Size: 163 312 52 273 95 57 126 339
Cuts at: 1417 1573 1902
Size: 156 329
Fragments arranged by size:
339 329 312 273 163 156 126 95
57 52
BspEI T'CCGG_A
Cuts at: 0 1886 1902
Size: 1886 16
BspHI T'CATG_A
Cuts at: 0 92 1902
Size: 92 1810
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 239 1902
Size: 158 81 1663
Fragments arranged by size:
1663 158 81
BsrBI CCG'CTC
Cuts at: 0 771 1902
Size: 771 1131
BsrFI r'CCGG_y
Cuts at: 0 326 347 832 877 1323 1351 1902
Size: 326 21 485 45 446 28 551
Fragments arranged by size:
551 485 446 326 45 28 21
BsrGI T'GTAC_A
Cuts at: 0 1559 1902
Size: 1559 343
BssSI C'ACGA_G
Cuts at: 0 1214 1902
Size: 1214 688
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1047 1902
Size: 1047 855
BstBI TT'CG_AA
Cuts at: 0 10 1902
Size: 10 1892
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 1740 1902
Size: 181 1559 162
Fragments arranged by size:
1559 181 162
BtgI C'CryG_G
Cuts at: 0 19 224 291 422 1019 1620 1902
Size: 19 205 67 131 597 601 282
Fragments arranged by size:
601 597 282 205 131 67 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 959 1296 1902
Size: 959 337 606
Fragments arranged by size:
959 606 337
BtsI GCAGTG_nn'
Cuts at: 0 513 1902
Size: 513 1389
ClaI AT'CG_AT
Cuts at: 0 1297 1902
Size: 1297 605
DrdI GACnn_nn'nnGTC
Cuts at: 0 1701 1902
Size: 1701 201
EaeI y'GGCC_r
Cuts at: 0 324 845 879 1287 1361 1481 1595 1742
Size: 324 521 34 408 74 120 114 147
Cuts at: 1742 1859 1902
Size: 117 43
Fragments arranged by size:
521 408 324 147 120 117 114 74
43 34
EarI CTCTTCn'nnn_
Cuts at: 0 560 1902
Size: 560 1342
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 79 447 1159 1902
Size: 79 368 712 743
Fragments arranged by size:
743 712 368 79
EcoICRI GAG'CTC
Cuts at: 0 1415 1902
Size: 1415 487
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 118 456 807 1057 1533 1534 1902
Size: 112 6 338 351 250 476 1 368
Fragments arranged by size:
476 368 351 338 250 112 6 1
EcoO109I rG'GnC_Cy
Cuts at: 0 99 160 188 512 651 731 1902
Size: 99 61 28 324 139 80 1171
Fragments arranged by size:
1171 324 139 99 80 61 28
HaeII r_GCGC'y
Cuts at: 0 878 1165 1205 1324 1352 1902
Size: 878 287 40 119 28 550
Fragments arranged by size:
878 550 287 119 40 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 1015 1047 1300 1332 1722 1754
Size: 559 32 424 32 253 32 390 32
Cuts at: 1754 1902
Size: 148
Fragments arranged by size:
559 424 390 253 148 32 32 32
32
HincII GTy'rAC
Cuts at: 0 548 1902
Size: 548 1354
HpaI GTT'AAC
Cuts at: 0 548 1902
Size: 548 1354
KasI G'GCGC_C
Cuts at: 0 874 1161 1320 1902
Size: 874 287 159 582
Fragments arranged by size:
874 582 287 159
KpnI G_GTAC'C
Cuts at: 0 1252 1902
Size: 1252 650
MluI A'CGCG_T
Cuts at: 0 1457 1902
Size: 1457 445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1796 1902
Size: 1796 106
MscI TGG'CCA
Cuts at: 0 1289 1902
Size: 1289 613
MspA1I CmG'CkG
Cuts at: 0 21 389 424 434 783 925 993 1047
Size: 21 368 35 10 349 142 68 54
Cuts at: 1047 1512 1902
Size: 465 390
Fragments arranged by size:
465 390 368 349 142 68 54 35
21 10
NaeI GCC'GGC
Cuts at: 0 328 349 879 1325 1353 1902
Size: 328 21 530 446 28 549
Fragments arranged by size:
549 530 446 328 28 21
NarI GG'CG_CC
Cuts at: 0 875 1162 1321 1902
Size: 875 287 159 581
Fragments arranged by size:
875 581 287 159
NcoI C'CATG_G
Cuts at: 0 224 291 1019 1902
Size: 224 67 728 883
Fragments arranged by size:
883 728 224 67
NgoMIV G'CCGG_C
Cuts at: 0 326 347 877 1323 1351 1902
Size: 326 21 530 446 28 551
Fragments arranged by size:
551 530 446 326 28 21
NspI r_CATG'y
Cuts at: 0 1836 1902
Size: 1836 66
PasI CC'CwG_GG
Cuts at: 0 1408 1437 1902
Size: 1408 29 465
Fragments arranged by size:
1408 465 29
PflMI CCAn_nnn'nTGG
Cuts at: 0 297 1512 1902
Size: 297 1215 390
Fragments arranged by size:
1215 390 297
PpuMI rG'GwC_Cy
Cuts at: 0 99 188 512 651 731 1902
Size: 99 89 324 139 80 1171
Fragments arranged by size:
1171 324 139 99 89 80
PspOMI G'GGCC_C
Cuts at: 0 159 1902
Size: 159 1743
PstI C_TGCA'G
Cuts at: 0 1334 1902
Size: 1334 568
PvuII CAG'CTG
Cuts at: 0 389 434 925 1047 1512 1902
Size: 389 45 491 122 465 390
Fragments arranged by size:
491 465 390 389 122 45
SacI G_AGCT'C
Cuts at: 0 1417 1902
Size: 1417 485
SacII CC_GC'GG
Cuts at: 0 22 425 1902
Size: 22 403 1477
Fragments arranged by size:
1477 403 22
SanDI GG'GwC_CC
Cuts at: 0 188 512 731 1902
Size: 188 324 219 1171
Fragments arranged by size:
1171 324 219 188
ScaI AGT'ACT
Cuts at: 0 178 1902
Size: 178 1724
SfcI C'TryA_G
Cuts at: 0 695 1013 1330 1902
Size: 695 318 317 572
Fragments arranged by size:
695 572 318 317
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1489 1902
Size: 1489 413
SfoI GGC'GCC
Cuts at: 0 876 1163 1322 1902
Size: 876 287 159 580
Fragments arranged by size:
876 580 287 159
SmaI CCC'GGG
Cuts at: 0 1568 1902
Size: 1568 334
SmlI C'TyrA_G
Cuts at: 0 1798 1902
Size: 1798 104
SrfI GCCC'GGGC
Cuts at: 0 1568 1902
Size: 1568 334
StyI C'CwwG_G
Cuts at: 0 224 291 1019 1902
Size: 224 67 728 883
Fragments arranged by size:
883 728 224 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 730 1014 1081 1902
Size: 730 284 67 821
Fragments arranged by size:
821 730 284 67
TatI w'GTAC_w
Cuts at: 0 176 1559 1902
Size: 176 1383 343
Fragments arranged by size:
1383 343 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 748 780 1902
Size: 748 32 1122
Fragments arranged by size:
1122 748 32
Tth111I GACn'n_nGTC
Cuts at: 0 627 1902
Size: 627 1275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 214 1902
Size: 214 1688
XmaI C'CCGG_G
Cuts at: 0 1566 1902
Size: 1566 336
ZraI GAC'GTC
Cuts at: 0 1758 1902
Size: 1758 144
Enzymes that do cut:
AatII Acc65I AflIII AhdI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BciVI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BtgI BtgZI BtsI ClaI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PpuMI
PspOMI PstI PvuII SacI SacII SanDI ScaI SfcI
SfiI SfoI SmaI SmlI SrfI StyI TaqII TatI
TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AgeI AleI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BcgI BclI BfrBI BglII BlpI BmtI BplI BsaAI
BsiEI BsiWI BsmI BsrDI BssHII BstEII BstYI BstZ17I
Bsu36I CspCI DraI DraIII EagI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII MfeI MslI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SapI SbfI SexAI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI XbaI XhoI XmnI