Restriction map for
pF1KB9937
(Linear) (Six-base) MAPSORT of: KIBB9937.seq Check: 76 from: 1 to: 1566
KIBB9937.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 0 782 1566
Size: 782 784
Acc65I G'GTAC_C
Cuts at: 0 731 823 1566
Size: 731 92 743
Fragments arranged by size:
743 731 92
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1566
Size: 721 845
AfeI AGC'GCT
Cuts at: 0 27 964 1022 1255 1566
Size: 27 937 58 233 311
Fragments arranged by size:
937 311 233 58 27
AlwNI CAG_nnn'CTG
Cuts at: 0 1518 1566
Size: 1518 48
ApoI r'AATT_y
Cuts at: 0 366 697 1566
Size: 366 331 869
Fragments arranged by size:
869 366 331
AvaI C'yCGr_G
Cuts at: 0 100 1360 1373 1566
Size: 100 1260 13 193
Fragments arranged by size:
1260 193 100 13
BamHI G'GATC_C
Cuts at: 0 227 1566
Size: 227 1339
BanI G'GyrC_C
Cuts at: 0 145 731 823 1566
Size: 145 586 92 743
Fragments arranged by size:
743 586 145 92
BanII G_rGCy'C
Cuts at: 0 1069 1184 1566
Size: 1069 115 382
Fragments arranged by size:
1069 382 115
BbeI G_GCGC'C
Cuts at: 0 149 1566
Size: 149 1417
BbsI GAAGACnn'nnnn_
Cuts at: 0 1249 1566
Size: 1249 317
BbvCI CC'TCA_GC
Cuts at: 0 1112 1566
Size: 1112 454
BclI T'GATC_A
Cuts at: 0 1478 1566
Size: 1478 88
BfrBI ATG'CAT
Cuts at: 0 481 1566
Size: 481 1085
BlpI GC'TnA_GC
Cuts at: 0 1178 1566
Size: 1178 388
Bme1580I G_kGCm'C
Cuts at: 0 1098 1566
Size: 1098 468
BmrI ACTGGGnnnn_n'
Cuts at: 0 1029 1539 1566
Size: 1029 510 27
Fragments arranged by size:
1029 510 27
BmtI G_CTAG'C
Cuts at: 0 328 1021 1566
Size: 328 693 545
Fragments arranged by size:
693 545 328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 59 618 1566
Size: 59 559 948
Fragments arranged by size:
948 559 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 922 1566
Size: 82 840 644
Fragments arranged by size:
840 644 82
Bpu10I CC'TnA_GC
Cuts at: 0 1112 1566
Size: 1112 454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 1510 1566
Size: 838 672 56
Fragments arranged by size:
838 672 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 853 1566
Size: 853 713
BsaAI yAC'GTr
Cuts at: 0 814 1476 1566
Size: 814 662 90
Fragments arranged by size:
814 662 90
BsaHI Gr'CG_yC
Cuts at: 0 4 146 779 1566
Size: 4 142 633 787
Fragments arranged by size:
787 633 142 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 196 226 934 964 1566
Size: 55 30 111 30 708 30 602
Fragments arranged by size:
708 602 111 55 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 341 409 841 943 970 1566
Size: 341 68 432 102 27 596
Fragments arranged by size:
596 432 341 102 68 27
BseYI C'CCAG_C
Cuts at: 0 106 380 1103 1566
Size: 106 274 723 463
Fragments arranged by size:
723 463 274 106
BsiEI CG_ry'CG
Cuts at: 0 86 1566
Size: 86 1480
BsiHKAI G_wGCw'C
Cuts at: 0 1069 1184 1566
Size: 1069 115 382
Fragments arranged by size:
1069 382 115
BsmI GAATG_Cn'
Cuts at: 0 483 1566
Size: 483 1083
Bsp1286I G_dGCh'C
Cuts at: 0 1069 1098 1184 1566
Size: 1069 29 86 382
Fragments arranged by size:
1069 382 86 29
BsrFI r'CCGG_y
Cuts at: 0 142 1566
Size: 142 1424
BstBI TT'CG_AA
Cuts at: 0 373 1566
Size: 373 1193
BstYI r'GATC_y
Cuts at: 0 227 258 1355 1566
Size: 227 31 1097 211
Fragments arranged by size:
1097 227 211 31
Bsu36I CC'TnA_GG
Cuts at: 0 353 1547 1566
Size: 353 1194 19
Fragments arranged by size:
1194 353 19
BtgI C'CryG_G
Cuts at: 0 149 818 1566
Size: 149 669 748
Fragments arranged by size:
748 669 149
BtsI GCAGTG_nn'
Cuts at: 0 1276 1566
Size: 1276 290
DrdI GACnn_nn'nnGTC
Cuts at: 0 778 1566
Size: 778 788
EaeI y'GGCC_r
Cuts at: 0 83 1208 1566
Size: 83 1125 358
Fragments arranged by size:
1125 358 83
EagI C'GGCC_G
Cuts at: 0 83 1566
Size: 83 1483
EarI CTCTTCn'nnn_
Cuts at: 0 350 634 797 1566
Size: 350 284 163 769
Fragments arranged by size:
769 350 284 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 35 1566
Size: 35 1531
EcoICRI GAG'CTC
Cuts at: 0 1067 1182 1566
Size: 1067 115 384
Fragments arranged by size:
1067 384 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 721 922 1566
Size: 82 639 201 644
Fragments arranged by size:
644 639 201 82
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1546 1566
Size: 1546 20
EcoO109I rG'GnC_Cy
Cuts at: 0 771 1566
Size: 771 795
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1558 1566
Size: 1558 8
HaeII r_GCGC'y
Cuts at: 0 29 149 966 1024 1257 1566
Size: 29 120 817 58 233 309
Fragments arranged by size:
817 309 233 120 58 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 735 767 1354 1386 1566
Size: 55 32 648 32 587 32 180
Fragments arranged by size:
648 587 180 55 32 32 32
HincII GTy'rAC
Cuts at: 0 1002 1566
Size: 1002 564
HindIII A'AGCT_T
Cuts at: 0 513 586 1566
Size: 513 73 980
Fragments arranged by size:
980 513 73
KasI G'GCGC_C
Cuts at: 0 145 1566
Size: 145 1421
KpnI G_GTAC'C
Cuts at: 0 735 827 1566
Size: 735 92 739
Fragments arranged by size:
739 735 92
MscI TGG'CCA
Cuts at: 0 1210 1566
Size: 1210 356
MslI CAynn'nnrTG
Cuts at: 0 652 901 1566
Size: 652 249 665
Fragments arranged by size:
665 652 249
MspA1I CmG'CkG
Cuts at: 0 151 1107 1518 1566
Size: 151 956 411 48
Fragments arranged by size:
956 411 151 48
NarI GG'CG_CC
Cuts at: 0 146 1566
Size: 146 1420
NdeI CA'TA_TG
Cuts at: 0 483 1566
Size: 483 1083
NheI G'CTAG_C
Cuts at: 0 324 1017 1566
Size: 324 693 549
Fragments arranged by size:
693 549 324
NsiI A_TGCA'T
Cuts at: 0 483 1566
Size: 483 1083
NspI r_CATG'y
Cuts at: 0 982 1566
Size: 982 584
PfoI T'CCnGG_A
Cuts at: 0 938 1566
Size: 938 628
PmlI CAC'GTG
Cuts at: 0 814 1476 1566
Size: 814 662 90
Fragments arranged by size:
814 662 90
PpuMI rG'GwC_Cy
Cuts at: 0 771 1566
Size: 771 795
PspXI vC'TCGA_Gb
Cuts at: 0 1360 1566
Size: 1360 206
PstI C_TGCA'G
Cuts at: 0 696 1283 1340 1566
Size: 696 587 57 226
Fragments arranged by size:
696 587 226 57
PvuII CAG'CTG
Cuts at: 0 1107 1518 1566
Size: 1107 411 48
Fragments arranged by size:
1107 411 48
SacI G_AGCT'C
Cuts at: 0 1069 1184 1566
Size: 1069 115 382
Fragments arranged by size:
1069 382 115
SacII CC_GC'GG
Cuts at: 0 152 1566
Size: 152 1414
SapI GCTCTTCn'nnn_
Cuts at: 0 634 797 1566
Size: 634 163 769
Fragments arranged by size:
769 634 163
ScaI AGT'ACT
Cuts at: 0 1528 1566
Size: 1528 38
SfcI C'TryA_G
Cuts at: 0 21 692 1279 1336 1566
Size: 21 671 587 57 230
Fragments arranged by size:
671 587 230 57 21
SfoI GGC'GCC
Cuts at: 0 147 1566
Size: 147 1419
SgrAI Cr'CCGG_yG
Cuts at: 0 142 1566
Size: 142 1424
SmlI C'TyrA_G
Cuts at: 0 853 1360 1489 1566
Size: 853 507 129 77
Fragments arranged by size:
853 507 129 77
SphI G_CATG'C
Cuts at: 0 982 1566
Size: 982 584
StyI C'CwwG_G
Cuts at: 0 290 1346 1457 1566
Size: 290 1056 111 109
Fragments arranged by size:
1056 290 111 109
TatI w'GTAC_w
Cuts at: 0 1526 1566
Size: 1526 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 934 966 1566
Size: 934 32 600
Fragments arranged by size:
934 600 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 835 1566
Size: 835 731
XhoI C'TCGA_G
Cuts at: 0 1360 1566
Size: 1360 206
XmnI GAAnn'nnTTC
Cuts at: 0 613 1566
Size: 613 953
ZraI GAC'GTC
Cuts at: 0 780 1566
Size: 780 786
Enzymes that do cut:
AatII Acc65I AcuI AfeI AlwNI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BclI BfrBI BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BsrFI BstBI BstYI Bsu36I BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HindIII KasI KpnI MscI
MslI MspA1I NarI NdeI NheI NsiI NspI PfoI
PmlI PpuMI PspXI PstI PvuII SacI SacII SapI
ScaI SfcI SfoI SgrAI SmlI SphI StyI TatI
TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BcgI BciVI BglI BglII BmgBI BsaBI BsaWI
BsgI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DraIII EcoRI EcoRV FseI FspI
FspAI HpaI MfeI MluI MmeI NaeI NcoI NgoMIV
NotI NruI PacI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SalI SanDI
SbfI SexAI SfiI SgfI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XmaI