Restriction map for
pF1KB9947
(Linear) (Six-base) MAPSORT of: KIBB9947.seq Check: 2593 from: 1 to: 1593
KIBB9947.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 706 1049 1593
Size: 421 285 343 544
Fragments arranged by size:
544 421 343 285
AflIII A'CryG_T
Cuts at: 0 86 1593
Size: 86 1507
AhdI GACnn_n'nnGTC
Cuts at: 0 1379 1593
Size: 1379 214
AleI CACnn'nnGTG
Cuts at: 0 1237 1593
Size: 1237 356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 581 1157 1593
Size: 581 576 436
Fragments arranged by size:
581 576 436
AlwNI CAG_nnn'CTG
Cuts at: 0 51 684 990 1137 1159 1593
Size: 51 633 306 147 22 434
Fragments arranged by size:
633 434 306 147 51 22
AvaI C'yCGr_G
Cuts at: 0 1022 1496 1593
Size: 1022 474 97
Fragments arranged by size:
1022 474 97
BanI G'GyrC_C
Cuts at: 0 565 1203 1243 1544 1593
Size: 565 638 40 301 49
Fragments arranged by size:
638 565 301 49 40
BanII G_rGCy'C
Cuts at: 0 368 1593
Size: 368 1225
BbeI G_GCGC'C
Cuts at: 0 1207 1548 1593
Size: 1207 341 45
Fragments arranged by size:
1207 341 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 909 1593
Size: 909 684
BbvCI CC'TCA_GC
Cuts at: 0 1448 1593
Size: 1448 145
BclI T'GATC_A
Cuts at: 0 1001 1593
Size: 1001 592
BglI GCCn_nnn'nGGC
Cuts at: 0 1150 1252 1593
Size: 1150 102 341
Fragments arranged by size:
1150 341 102
BglII A'GATC_T
Cuts at: 0 518 1593
Size: 518 1075
Bme1580I G_kGCm'C
Cuts at: 0 1344 1593
Size: 1344 249
BmgBI CAC'GTC
Cuts at: 0 1455 1593
Size: 1455 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 371 1423 1593
Size: 371 1052 170
Fragments arranged by size:
1052 371 170
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 1593
Size: 433 1160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 532 871 1012 1106 1135 1593
Size: 100 432 339 141 94 29 458
Fragments arranged by size:
458 432 339 141 100 94 29
Bpu10I CC'TnA_GC
Cuts at: 0 1448 1593
Size: 1448 145
BsaI GGTCTCn'nnnn_
Cuts at: 0 250 1593
Size: 250 1343
BsaAI yAC'GTr
Cuts at: 0 525 1339 1593
Size: 525 814 254
Fragments arranged by size:
814 525 254
BsaHI Gr'CG_yC
Cuts at: 0 1204 1545 1593
Size: 1204 341 48
Fragments arranged by size:
1204 341 48
BsaWI w'CCGG_w
Cuts at: 0 1537 1593
Size: 1537 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 1508 1538 1593
Size: 73 30 1405 30 55
Fragments arranged by size:
1405 73 55 30 30
BseYI C'CCAG_C
Cuts at: 0 44 156 958 1593
Size: 44 112 802 635
Fragments arranged by size:
802 635 112 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1593
Size: 37 1556
BsiHKAI G_wGCw'C
Cuts at: 0 86 1593
Size: 86 1507
BsmBI CGTCTCn'nnnn_
Cuts at: 0 169 1593
Size: 169 1424
Bsp1286I G_dGCh'C
Cuts at: 0 86 368 1344 1593
Size: 86 282 976 249
Fragments arranged by size:
976 282 249 86
BspHI T'CATG_A
Cuts at: 0 240 819 1593
Size: 240 579 774
Fragments arranged by size:
774 579 240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1295 1593
Size: 1295 298
BsrDI GCAATG_nn'
Cuts at: 0 1349 1593
Size: 1349 244
BsrFI r'CCGG_y
Cuts at: 0 314 1593
Size: 314 1279
BssSI C'ACGA_G
Cuts at: 0 878 1593
Size: 878 715
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1159 1593
Size: 1159 434
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1239 1593
Size: 1239 354
BstYI r'GATC_y
Cuts at: 0 353 518 681 1593
Size: 353 165 163 912
Fragments arranged by size:
912 353 165 163
BtgI C'CryG_G
Cuts at: 0 62 281 1238 1247 1398 1593
Size: 62 219 957 9 151 195
Fragments arranged by size:
957 219 195 151 62 9
BtsI GCAGTG_nn'
Cuts at: 0 383 1041 1278 1593
Size: 383 658 237 315
Fragments arranged by size:
658 383 315 237
DraIII CAC_nnn'GTG
Cuts at: 0 1320 1593
Size: 1320 273
EaeI y'GGCC_r
Cuts at: 0 1043 1322 1593
Size: 1043 279 271
Fragments arranged by size:
1043 279 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1183 1593
Size: 1183 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 421 532 706 871 1012 1049 1106
Size: 100 321 111 174 165 141 37 57
Cuts at: 1106 1135 1593
Size: 29 458
Fragments arranged by size:
458 321 174 165 141 111 100 57
37 29
EcoO109I rG'GnC_Cy
Cuts at: 0 346 768 1425 1593
Size: 346 422 657 168
Fragments arranged by size:
657 422 346 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 311 1593
Size: 311 1282
FspI TGC'GCA
Cuts at: 0 274 1126 1593
Size: 274 852 467
Fragments arranged by size:
852 467 274
FspAI rTGC'GCAy
Cuts at: 0 274 1126 1593
Size: 274 852 467
Fragments arranged by size:
852 467 274
HaeII r_GCGC'y
Cuts at: 0 1207 1548 1593
Size: 1207 341 45
Fragments arranged by size:
1207 341 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 338 370 1350 1365 1382 1397 1508 1540
Size: 338 32 980 15 17 15 111 32
Cuts at: 1540 1593
Size: 53
Fragments arranged by size:
980 338 111 53 32 32 17 15
15
HindIII A'AGCT_T
Cuts at: 0 288 1593
Size: 288 1305
KasI G'GCGC_C
Cuts at: 0 1203 1544 1593
Size: 1203 341 49
Fragments arranged by size:
1203 341 49
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 1593
Size: 644 949
MscI TGG'CCA
Cuts at: 0 1045 1324 1593
Size: 1045 279 269
Fragments arranged by size:
1045 279 269
MslI CAynn'nnrTG
Cuts at: 0 1237 1354 1593
Size: 1237 117 239
Fragments arranged by size:
1237 239 117
MspA1I CmG'CkG
Cuts at: 0 51 380 1593
Size: 51 329 1213
Fragments arranged by size:
1213 329 51
NarI GG'CG_CC
Cuts at: 0 1204 1545 1593
Size: 1204 341 48
Fragments arranged by size:
1204 341 48
NcoI C'CATG_G
Cuts at: 0 62 1593
Size: 62 1531
NspI r_CATG'y
Cuts at: 0 90 1131 1593
Size: 90 1041 462
Fragments arranged by size:
1041 462 90
PciI A'CATG_T
Cuts at: 0 86 1593
Size: 86 1507
PfoI T'CCnGG_A
Cuts at: 0 77 677 848 1055 1433 1593
Size: 77 600 171 207 378 160
Fragments arranged by size:
600 378 207 171 160 77
PmlI CAC'GTG
Cuts at: 0 1339 1593
Size: 1339 254
PpuMI rG'GwC_Cy
Cuts at: 0 346 768 1425 1593
Size: 346 422 657 168
Fragments arranged by size:
657 422 346 168
PstI C_TGCA'G
Cuts at: 0 390 909 1273 1593
Size: 390 519 364 320
Fragments arranged by size:
519 390 364 320
PvuII CAG'CTG
Cuts at: 0 51 1593
Size: 51 1542
SexAI A'CCwGG_T
Cuts at: 0 1316 1593
Size: 1316 277
SfcI C'TryA_G
Cuts at: 0 386 905 1269 1593
Size: 386 519 364 324
Fragments arranged by size:
519 386 364 324
SfoI GGC'GCC
Cuts at: 0 1205 1546 1593
Size: 1205 341 47
Fragments arranged by size:
1205 341 47
SphI G_CATG'C
Cuts at: 0 1131 1593
Size: 1131 462
StuI AGG'CCT
Cuts at: 0 1255 1441 1593
Size: 1255 186 152
Fragments arranged by size:
1255 186 152
StyI C'CwwG_G
Cuts at: 0 62 605 1593
Size: 62 543 988
Fragments arranged by size:
988 543 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 129 1231 1593
Size: 129 1102 362
Fragments arranged by size:
1102 362 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 194 226 1593
Size: 73 32 89 32 1367
Fragments arranged by size:
1367 89 73 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1429 1593
Size: 1429 164
XbaI T'CTAG_A
Cuts at: 0 333 1593
Size: 333 1260
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1053 1396 1593
Size: 1053 343 197
Fragments arranged by size:
1053 343 197
XmnI GAAnn'nnTTC
Cuts at: 0 916 1593
Size: 916 677
Enzymes that do cut:
AcuI AflIII AhdI AleI AlfI AlwNI AvaI BanI
BanII BbeI BbsI BbvCI BclI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI
BtsI DraIII EaeI EciI Eco57MI EcoO109I FalI FspI
FspAI HaeII Hin4I HindIII KasI MmeI MscI MslI
MspA1I NarI NcoI NspI PciI PfoI PmlI PpuMI
PstI PvuII SexAI SfcI SfoI SphI StuI StyI
TaqII TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BfrBI BlpI BmtI BpuEI
BsaBI BseRI BsiEI BsiWI BsmI BspEI BsrBI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV
FseI HincII HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI TatI XhoI XmaI ZraI