Restriction map for
pF1KB9951
(Linear) (Six-base) MAPSORT of: KIBB9951.seq Check: 4163 from: 1 to: 1605
KIBB9951.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 493 505 1417 1605
Size: 50 443 12 912 188
Fragments arranged by size:
912 443 188 50 12
AflIII A'CryG_T
Cuts at: 0 455 1605
Size: 455 1150
AleI CACnn'nnGTG
Cuts at: 0 578 754 1605
Size: 578 176 851
Fragments arranged by size:
851 578 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1348 1605
Size: 1348 257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 950 982 1605
Size: 950 32 623
Fragments arranged by size:
950 623 32
AlwNI CAG_nnn'CTG
Cuts at: 0 73 1588 1605
Size: 73 1515 17
Fragments arranged by size:
1515 73 17
ApaLI G'TGCA_C
Cuts at: 0 540 794 1605
Size: 540 254 811
Fragments arranged by size:
811 540 254
ApoI r'AATT_y
Cuts at: 0 1174 1605
Size: 1174 431
AvaI C'yCGr_G
Cuts at: 0 1459 1605
Size: 1459 146
BanI G'GyrC_C
Cuts at: 0 1421 1605
Size: 1421 184
BbsI GAAGACnn'nnnn_
Cuts at: 0 496 697 893 1605
Size: 496 201 196 712
Fragments arranged by size:
712 496 201 196
BbvCI CC'TCA_GC
Cuts at: 0 114 1310 1605
Size: 114 1196 295
Fragments arranged by size:
1196 295 114
BciVI GTATCCnnnnn_n'
Cuts at: 0 252 1074 1605
Size: 252 822 531
Fragments arranged by size:
822 531 252
BglI GCCn_nnn'nGGC
Cuts at: 0 183 1096 1605
Size: 183 913 509
Fragments arranged by size:
913 509 183
BlpI GC'TnA_GC
Cuts at: 0 1472 1605
Size: 1472 133
Bme1580I G_kGCm'C
Cuts at: 0 544 798 992 1277 1605
Size: 544 254 194 285 328
Fragments arranged by size:
544 328 285 254 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 835 1605
Size: 820 15 770
Fragments arranged by size:
820 770 15
Bpu10I CC'TnA_GC
Cuts at: 0 114 562 1310 1426 1605
Size: 114 448 748 116 179
Fragments arranged by size:
748 448 179 116 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 1531 1605
Size: 1531 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 559 589 763 793 1605
Size: 28 531 30 174 30 812
Fragments arranged by size:
812 531 174 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1075 1267 1378 1590 1605
Size: 1075 192 111 212 15
Fragments arranged by size:
1075 212 192 111 15
BseYI C'CCAG_C
Cuts at: 0 972 1235 1485 1605
Size: 972 263 250 120
Fragments arranged by size:
972 263 250 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1501 1605
Size: 556 945 104
Fragments arranged by size:
945 556 104
BsiHKAI G_wGCw'C
Cuts at: 0 544 798 1605
Size: 544 254 807
Fragments arranged by size:
807 544 254
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1602 1605
Size: 1602 3
Bsp1286I G_dGCh'C
Cuts at: 0 544 798 992 1277 1605
Size: 544 254 194 285 328
Fragments arranged by size:
544 328 285 254 194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 578 1605
Size: 578 1027
BsrDI GCAATG_nn'
Cuts at: 0 834 1605
Size: 834 771
BsrFI r'CCGG_y
Cuts at: 0 1418 1514 1605
Size: 1418 96 91
Fragments arranged by size:
1418 96 91
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1077 1605
Size: 1077 528
BstYI r'GATC_y
Cuts at: 0 1199 1605
Size: 1199 406
BtgI C'CryG_G
Cuts at: 0 254 597 1605
Size: 254 343 1008
Fragments arranged by size:
1008 343 254
BtsI GCAGTG_nn'
Cuts at: 0 39 773 815 873 1014 1348 1605
Size: 39 734 42 58 141 334 257
Fragments arranged by size:
734 334 257 141 58 42 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 749 784 1605
Size: 749 35 821
Fragments arranged by size:
821 749 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 87 1605
Size: 87 1518
EaeI y'GGCC_r
Cuts at: 0 251 1605
Size: 251 1354
EarI CTCTTCn'nnn_
Cuts at: 0 164 190 200 698 1116 1529 1605
Size: 164 26 10 498 418 413 76
Fragments arranged by size:
498 418 413 164 76 26 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 493 505 820 835 1417 1605
Size: 50 443 12 315 15 582 188
Fragments arranged by size:
582 443 315 188 50 15 12
HaeII r_GCGC'y
Cuts at: 0 189 1605
Size: 189 1416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 763 795 1191 1223 1246 1278 1605
Size: 30 733 32 396 32 23 32 327
Fragments arranged by size:
733 396 327 32 32 32 30 23
HincII GTy'rAC
Cuts at: 0 1520 1605
Size: 1520 85
MfeI C'AATT_G
Cuts at: 0 367 1605
Size: 367 1238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1318 1382 1605
Size: 1318 64 223
Fragments arranged by size:
1318 223 64
MscI TGG'CCA
Cuts at: 0 253 1605
Size: 253 1352
MslI CAynn'nnrTG
Cuts at: 0 578 754 1605
Size: 578 176 851
Fragments arranged by size:
851 578 176
MspA1I CmG'CkG
Cuts at: 0 118 1309 1485 1588 1605
Size: 118 1191 176 103 17
Fragments arranged by size:
1191 176 118 103 17
NcoI C'CATG_G
Cuts at: 0 254 597 1605
Size: 254 343 1008
Fragments arranged by size:
1008 343 254
NdeI CA'TA_TG
Cuts at: 0 843 1605
Size: 843 762
NspI r_CATG'y
Cuts at: 0 459 792 1605
Size: 459 333 813
Fragments arranged by size:
813 459 333
PciI A'CATG_T
Cuts at: 0 455 1605
Size: 455 1150
PflMI CCAn_nnn'nTGG
Cuts at: 0 739 1605
Size: 739 866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 1115 1147 1605
Size: 283 32 800 32 458
Fragments arranged by size:
800 458 283 32 32
PstI C_TGCA'G
Cuts at: 0 329 926 1113 1605
Size: 329 597 187 492
Fragments arranged by size:
597 492 329 187
PvuII CAG'CTG
Cuts at: 0 118 1309 1485 1605
Size: 118 1191 176 120
Fragments arranged by size:
1191 176 120 118
SapI GCTCTTCn'nnn_
Cuts at: 0 698 1605
Size: 698 907
SbfI CC_TGCA'GG
Cuts at: 0 1113 1605
Size: 1113 492
ScaI AGT'ACT
Cuts at: 0 26 1605
Size: 26 1579
SexAI A'CCwGG_T
Cuts at: 0 228 1605
Size: 228 1377
SfcI C'TryA_G
Cuts at: 0 325 922 1109 1605
Size: 325 597 187 496
Fragments arranged by size:
597 496 325 187
SpeI A'CTAG_T
Cuts at: 0 1084 1605
Size: 1084 521
SspI AAT'ATT
Cuts at: 0 403 1605
Size: 403 1202
StyI C'CwwG_G
Cuts at: 0 254 597 1605
Size: 254 343 1008
Fragments arranged by size:
1008 343 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 986 1605
Size: 986 619
TatI w'GTAC_w
Cuts at: 0 24 1142 1605
Size: 24 1118 463
Fragments arranged by size:
1118 463 24
XbaI T'CTAG_A
Cuts at: 0 882 1056 1605
Size: 882 174 549
Fragments arranged by size:
882 549 174
XmnI GAAnn'nnTTC
Cuts at: 0 338 1130 1549 1605
Size: 338 792 419 56
Fragments arranged by size:
792 419 338 56
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI AlwNI ApaLI ApoI
AvaI BanI BbsI BbvCI BciVI BglI BlpI Bme1580I
BpmI Bpu10I BsaI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrDI BsrFI BstXI BstYI BtgI
BtsI CspCI DrdI EaeI EarI Eco57MI HaeII Hin4I
HincII MfeI MmeI MscI MslI MspA1I NcoI NdeI
NspI PciI PflMI PpiI PstI PvuII SapI SbfI
ScaI SexAI SfcI SpeI SspI StyI TaqII TatI
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbeI BcgI BclI BfrBI BglII BmgBI BmrI
BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI
DraIII EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SphI SrfI StuI SwaI TstI Tth111I XcmI XhoI
XmaI ZraI