Restriction map for
pF1KB9954
(Linear) (Six-base) MAPSORT of: KIBB9954.seq Check: 7942 from: 1 to: 2031
KIBB9954.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1839 2031
Size: 1839 192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 615 916 1348 2031
Size: 164 451 301 432 683
Fragments arranged by size:
683 451 432 301 164
AfeI AGC'GCT
Cuts at: 0 165 2031
Size: 165 1866
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 227 2000 2031
Size: 227 1773 31
Fragments arranged by size:
1773 227 31
ApoI r'AATT_y
Cuts at: 0 327 484 679 748 1028 1262 1350 1400
Size: 327 157 195 69 280 234 88 50
Cuts at: 1400 1407 2004 2031
Size: 7 597 27
Fragments arranged by size:
597 327 280 234 195 157 88 69
50 27 7
BamHI G'GATC_C
Cuts at: 0 1795 2031
Size: 1795 236
BbsI GAAGACnn'nnnn_
Cuts at: 0 14 152 784 1871 2031
Size: 14 138 632 1087 160
Fragments arranged by size:
1087 632 160 138 14
BciVI GTATCCnnnnn_n'
Cuts at: 0 1608 2031
Size: 1608 423
BclI T'GATC_A
Cuts at: 0 993 1494 1809 2031
Size: 993 501 315 222
Fragments arranged by size:
993 501 315 222
BfrBI ATG'CAT
Cuts at: 0 730 970 1005 2031
Size: 730 240 35 1026
Fragments arranged by size:
1026 730 240 35
BglI GCCn_nnn'nGGC
Cuts at: 0 104 182 2031
Size: 104 78 1849
Fragments arranged by size:
1849 104 78
BglII A'GATC_T
Cuts at: 0 1623 2031
Size: 1623 408
BlpI GC'TnA_GC
Cuts at: 0 1271 2031
Size: 1271 760
BmgBI CAC'GTC
Cuts at: 0 586 2031
Size: 586 1445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 524 2031
Size: 524 1507
Bpu10I CC'TnA_GC
Cuts at: 0 960 2031
Size: 960 1071
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 899 2031
Size: 899 1132
BsaI GGTCTCn'nnnn_
Cuts at: 0 260 2031
Size: 260 1771
BsaBI GATnn'nnATC
Cuts at: 0 890 2031
Size: 890 1141
BsaHI Gr'CG_yC
Cuts at: 0 1191 2031
Size: 1191 840
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 602 632 2031
Size: 602 30 1399
Fragments arranged by size:
1399 602 30
BseYI C'CCAG_C
Cuts at: 0 273 2031
Size: 273 1758
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1448 2031
Size: 1448 583
BsiHKAI G_wGCw'C
Cuts at: 0 411 1824 2031
Size: 411 1413 207
Fragments arranged by size:
1413 411 207
BsmBI CGTCTCn'nnnn_
Cuts at: 0 667 2031
Size: 667 1364
Bsp1286I G_dGCh'C
Cuts at: 0 411 1824 2031
Size: 411 1413 207
Fragments arranged by size:
1413 411 207
BspHI T'CATG_A
Cuts at: 0 237 651 990 2031
Size: 237 414 339 1041
Fragments arranged by size:
1041 414 339 237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1839 2031
Size: 1839 192
BsrBI CCG'CTC
Cuts at: 0 574 2031
Size: 574 1457
BsrDI GCAATG_nn'
Cuts at: 0 192 1007 2031
Size: 192 815 1024
Fragments arranged by size:
1024 815 192
BsrFI r'CCGG_y
Cuts at: 0 1193 2031
Size: 1193 838
BstEII G'GTnAC_C
Cuts at: 0 565 2031
Size: 565 1466
BstYI r'GATC_y
Cuts at: 0 1623 1795 2031
Size: 1623 172 236
Fragments arranged by size:
1623 236 172
BtgI C'CryG_G
Cuts at: 0 521 2031
Size: 521 1510
DraI TTT'AAA
Cuts at: 0 2001 2031
Size: 2001 30
EarI CTCTTCn'nnn_
Cuts at: 0 699 939 2031
Size: 699 240 1092
Fragments arranged by size:
1092 699 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 615 916 1348 2031
Size: 164 451 301 432 683
Fragments arranged by size:
683 451 432 301 164
EcoRI G'AATT_C
Cuts at: 0 1350 1400 2031
Size: 1350 50 631
Fragments arranged by size:
1350 631 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1329 2031
Size: 1329 702
HaeII r_GCGC'y
Cuts at: 0 167 2031
Size: 167 1864
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1064 1096 1479 1511 1635 1667 2031
Size: 1064 32 383 32 124 32 364
Fragments arranged by size:
1064 383 364 124 32 32 32
HincII GTy'rAC
Cuts at: 0 1229 1320 2031
Size: 1229 91 711
Fragments arranged by size:
1229 711 91
HindIII A'AGCT_T
Cuts at: 0 41 2031
Size: 41 1990
HpaI GTT'AAC
Cuts at: 0 1229 2031
Size: 1229 802
MfeI C'AATT_G
Cuts at: 0 908 2031
Size: 908 1123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 934 1873 1973 2031
Size: 117 817 939 100 58
Fragments arranged by size:
939 817 117 100 58
MslI CAynn'nnrTG
Cuts at: 0 209 236 2031
Size: 209 27 1795
Fragments arranged by size:
1795 209 27
MspA1I CmG'CkG
Cuts at: 0 273 697 2031
Size: 273 424 1334
Fragments arranged by size:
1334 424 273
NaeI GCC'GGC
Cuts at: 0 1195 2031
Size: 1195 836
NgoMIV G'CCGG_C
Cuts at: 0 1193 2031
Size: 1193 838
NsiI A_TGCA'T
Cuts at: 0 732 972 1007 2031
Size: 732 240 35 1024
Fragments arranged by size:
1024 732 240 35
NspI r_CATG'y
Cuts at: 0 953 2031
Size: 953 1078
PasI CC'CwG_GG
Cuts at: 0 1893 2031
Size: 1893 138
PstI C_TGCA'G
Cuts at: 0 129 515 2031
Size: 129 386 1516
Fragments arranged by size:
1516 386 129
PvuII CAG'CTG
Cuts at: 0 273 697 2031
Size: 273 424 1334
Fragments arranged by size:
1334 424 273
SapI GCTCTTCn'nnn_
Cuts at: 0 699 939 2031
Size: 699 240 1092
Fragments arranged by size:
1092 699 240
SfcI C'TryA_G
Cuts at: 0 125 511 815 1113 2031
Size: 125 386 304 298 918
Fragments arranged by size:
918 386 304 298 125
SmlI C'TyrA_G
Cuts at: 0 914 2031
Size: 914 1117
StyI C'CwwG_G
Cuts at: 0 62 621 720 2031
Size: 62 559 99 1311
Fragments arranged by size:
1311 559 99 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 553 584 824 2031
Size: 553 31 240 1207
Fragments arranged by size:
1207 553 240 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 602 634 1410 1442 1830 1862 2031
Size: 602 32 776 32 388 32 169
Fragments arranged by size:
776 602 388 169 32 32 32
Enzymes that do cut:
AarI AcuI AfeI AlfI ApoI BamHI BbsI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BplI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaXI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstEII
BstYI BtgI DraI EarI Eco57MI EcoRI FalI HaeII
Hin4I HincII HindIII HpaI MfeI MmeI MslI MspA1I
NaeI NgoMIV NsiI NspI PasI PstI PvuII SapI
SfcI SmlI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BbvCI BcgI
Bme1580I BmrI BmtI BpmI BsaAI BsaWI BseRI BsiEI
BsiWI BsmI BspEI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI KasI KpnI MluI MscI NarI
NcoI NdeI NheI NotI NruI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI