Restriction map for
pF1KB9955
(Linear) (Six-base) MAPSORT of: KIBB9955.seq Check: 7986 from: 1 to: 1611
KIBB9955.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1422 1611
Size: 1422 189
Acc65I G'GTAC_C
Cuts at: 0 80 511 862 1611
Size: 80 431 351 749
Fragments arranged by size:
749 431 351 80
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 982 1541 1611
Size: 655 327 559 70
Fragments arranged by size:
655 559 327 70
AflIII A'CryG_T
Cuts at: 0 1343 1611
Size: 1343 268
AhdI GACnn_n'nnGTC
Cuts at: 0 184 1347 1611
Size: 184 1163 264
Fragments arranged by size:
1163 264 184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 729 1611
Size: 729 882
ApaI G_GGCC'C
Cuts at: 0 657 1611
Size: 657 954
ApoI r'AATT_y
Cuts at: 0 322 611 1611
Size: 322 289 1000
Fragments arranged by size:
1000 322 289
AvaI C'yCGr_G
Cuts at: 0 650 800 1303 1611
Size: 650 150 503 308
Fragments arranged by size:
650 503 308 150
BanI G'GyrC_C
Cuts at: 0 80 511 547 862 1611
Size: 80 431 36 315 749
Fragments arranged by size:
749 431 315 80 36
BanII G_rGCy'C
Cuts at: 0 76 657 1010 1083 1493 1586 1611
Size: 76 581 353 73 410 93 25
Fragments arranged by size:
581 410 353 93 76 73 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1253 1568 1611
Size: 1253 315 43
Fragments arranged by size:
1253 315 43
BbvCI CC'TCA_GC
Cuts at: 0 107 1611
Size: 107 1504
BciVI GTATCCnnnnn_n'
Cuts at: 0 628 1611
Size: 628 983
BclI T'GATC_A
Cuts at: 0 818 1611
Size: 818 793
BglI GCCn_nnn'nGGC
Cuts at: 0 1306 1611
Size: 1306 305
BglII A'GATC_T
Cuts at: 0 1167 1611
Size: 1167 444
BlpI GC'TnA_GC
Cuts at: 0 476 1611
Size: 476 1135
Bme1580I G_kGCm'C
Cuts at: 0 657 1611
Size: 657 954
BmgBI CAC'GTC
Cuts at: 0 1344 1611
Size: 1344 267
BmrI ACTGGGnnnn_n'
Cuts at: 0 651 838 1581 1611
Size: 651 187 743 30
Fragments arranged by size:
743 651 187 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 271 1611
Size: 271 1340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1447 1611
Size: 401 1046 164
Fragments arranged by size:
1046 401 164
Bpu10I CC'TnA_GC
Cuts at: 0 107 1611
Size: 107 1504
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1611
Size: 955 656
BsaI GGTCTCn'nnnn_
Cuts at: 0 584 715 1611
Size: 584 131 896
Fragments arranged by size:
896 584 131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 581 611 1611
Size: 581 30 1000
Fragments arranged by size:
1000 581 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 251 1018 1611
Size: 61 190 767 593
Fragments arranged by size:
767 593 190 61
BseYI C'CCAG_C
Cuts at: 0 118 400 1611
Size: 118 282 1211
Fragments arranged by size:
1211 282 118
BsiHKAI G_wGCw'C
Cuts at: 0 76 1083 1493 1611
Size: 76 1007 410 118
Fragments arranged by size:
1007 410 118 76
BsiWI C'GTAC_G
Cuts at: 0 229 1611
Size: 229 1382
BsmBI CGTCTCn'nnnn_
Cuts at: 0 215 1611
Size: 215 1396
Bsp1286I G_dGCh'C
Cuts at: 0 76 657 1010 1083 1493 1586 1611
Size: 76 581 353 73 410 93 25
Fragments arranged by size:
581 410 353 93 76 73 25
BspHI T'CATG_A
Cuts at: 0 950 1493 1611
Size: 950 543 118
Fragments arranged by size:
950 543 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 697 1422 1611
Size: 697 725 189
Fragments arranged by size:
725 697 189
BsrBI CCG'CTC
Cuts at: 0 368 1611
Size: 368 1243
BsrDI GCAATG_nn'
Cuts at: 0 19 1611
Size: 19 1592
BsrGI T'GTAC_A
Cuts at: 0 681 1611
Size: 681 930
BstEII G'GTnAC_C
Cuts at: 0 1377 1611
Size: 1377 234
BstXI CCAn_nnnn'nTGG
Cuts at: 0 76 1611
Size: 76 1535
BstYI r'GATC_y
Cuts at: 0 1167 1611
Size: 1167 444
BtsI GCAGTG_nn'
Cuts at: 0 998 1500 1611
Size: 998 502 111
Fragments arranged by size:
998 502 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1082 1117 1611
Size: 1082 35 494
Fragments arranged by size:
1082 494 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 976 1611
Size: 976 635
EaeI y'GGCC_r
Cuts at: 0 462 1611
Size: 462 1149
EarI CTCTTCn'nnn_
Cuts at: 0 210 434 1071 1383 1611
Size: 210 224 637 312 228
Fragments arranged by size:
637 312 228 224 210
EcoICRI GAG'CTC
Cuts at: 0 74 1081 1491 1611
Size: 74 1007 410 120
Fragments arranged by size:
1007 410 120 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 655 982 1447 1541 1611
Size: 401 254 327 465 94 70
Fragments arranged by size:
465 401 327 254 94 70
EcoO109I rG'GnC_Cy
Cuts at: 0 221 1515 1611
Size: 221 1294 96
Fragments arranged by size:
1294 221 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 170 202 581 613 1159 1191 1333 1365
Size: 170 32 379 32 546 32 142 32
Cuts at: 1365 1611
Size: 246
Fragments arranged by size:
546 379 246 170 142 32 32 32
32
HincII GTy'rAC
Cuts at: 0 423 1611
Size: 423 1188
KpnI G_GTAC'C
Cuts at: 0 84 515 866 1611
Size: 84 431 351 745
Fragments arranged by size:
745 431 351 84
MslI CAynn'nnrTG
Cuts at: 0 1189 1611
Size: 1189 422
NdeI CA'TA_TG
Cuts at: 0 1207 1611
Size: 1207 404
PflMI CCAn_nnn'nTGG
Cuts at: 0 1292 1611
Size: 1292 319
PfoI T'CCnGG_A
Cuts at: 0 1556 1611
Size: 1556 55
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 1611
Size: 58 32 1521
Fragments arranged by size:
1521 58 32
PpuMI rG'GwC_Cy
Cuts at: 0 221 1515 1611
Size: 221 1294 96
Fragments arranged by size:
1294 221 96
PsiI TTA'TAA
Cuts at: 0 608 1611
Size: 608 1003
PspOMI G'GGCC_C
Cuts at: 0 653 1611
Size: 653 958
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1309 1341 1611
Size: 1309 32 270
Fragments arranged by size:
1309 270 32
PstI C_TGCA'G
Cuts at: 0 708 815 1128 1611
Size: 708 107 313 483
Fragments arranged by size:
708 483 313 107
SacI G_AGCT'C
Cuts at: 0 76 1083 1493 1611
Size: 76 1007 410 118
Fragments arranged by size:
1007 410 118 76
SapI GCTCTTCn'nnn_
Cuts at: 0 1071 1611
Size: 1071 540
ScaI AGT'ACT
Cuts at: 0 1543 1611
Size: 1543 68
SexAI A'CCwGG_T
Cuts at: 0 1596 1611
Size: 1596 15
SfcI C'TryA_G
Cuts at: 0 704 811 1124 1446 1611
Size: 704 107 313 322 165
Fragments arranged by size:
704 322 313 165 107
SmaI CCC'GGG
Cuts at: 0 652 1611
Size: 652 959
SmlI C'TyrA_G
Cuts at: 0 800 934 1611
Size: 800 134 677
Fragments arranged by size:
800 677 134
SspI AAT'ATT
Cuts at: 0 332 1611
Size: 332 1279
StyI C'CwwG_G
Cuts at: 0 174 751 1611
Size: 174 577 860
Fragments arranged by size:
860 577 174
TatI w'GTAC_w
Cuts at: 0 681 1256 1541 1611
Size: 681 575 285 70
Fragments arranged by size:
681 575 285 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 233 265 548 580 1611
Size: 233 32 283 32 1031
Fragments arranged by size:
1031 283 233 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 595 1611
Size: 595 1016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 408 1594 1611
Size: 408 1186 17
Fragments arranged by size:
1186 408 17
XhoI C'TCGA_G
Cuts at: 0 800 1611
Size: 800 811
XmaI C'CCGG_G
Cuts at: 0 650 1611
Size: 650 961
XmnI GAAnn'nnTTC
Cuts at: 0 323 668 1611
Size: 323 345 943
Fragments arranged by size:
943 345 323
Enzymes that do cut:
AarI Acc65I AcuI AflIII AhdI AlfI ApaI ApoI
AvaI BanI BanII BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BseYI BsiHKAI BsiWI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstEII BstXI
BstYI BtsI CspCI DrdI EaeI EarI EcoICRI Eco57MI
EcoO109I Hin4I HincII KpnI MslI NdeI PflMI PfoI
PpiI PpuMI PsiI PspOMI PsrI PstI SacI SapI
ScaI SexAI SfcI SmaI SmlI SspI StyI TatI
TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AleI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BfrBI BmtI BsaAI BsaBI BsaHI BsaWI
BsgI BsiEI BsmI BspEI BsrFI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI MmeI
MscI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PshAI PspXI PvuI PvuII RsrII SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI StuI SwaI TaqII XbaI ZraI