Restriction map for
pF1KB9962
(Linear) (Six-base) MAPSORT of: KIBB9962.seq Check: 1245 from: 1 to: 2076
KIBB9962.orf
With 182 enzymes: SgfI *
September 25, 2012 15:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1442 2076
Size: 1442 634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 1144 2076
Size: 393 751 932
Fragments arranged by size:
932 751 393
AflIII A'CryG_T
Cuts at: 0 698 2076
Size: 698 1378
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 484 516 662 694 2076
Size: 484 32 146 32 1382
Fragments arranged by size:
1382 484 146 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1975 2076
Size: 1975 101
ApaI G_GGCC'C
Cuts at: 0 78 2076
Size: 78 1998
ApoI r'AATT_y
Cuts at: 0 524 836 1605 2076
Size: 524 312 769 471
Fragments arranged by size:
769 524 471 312
AvaI C'yCGr_G
Cuts at: 0 1891 2076
Size: 1891 185
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1244 1277 2076
Size: 1244 33 799
Fragments arranged by size:
1244 799 33
BanII G_rGCy'C
Cuts at: 0 78 132 217 592 1330 1423 1942 2076
Size: 78 54 85 375 738 93 519 134
Fragments arranged by size:
738 519 375 134 93 85 78 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 1073 2076
Size: 1073 1003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1312 1346 2076
Size: 1312 34 730
Fragments arranged by size:
1312 730 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 41 486 1791 2076
Size: 41 445 1305 285
Fragments arranged by size:
1305 445 285 41
BfrBI ATG'CAT
Cuts at: 0 763 2076
Size: 763 1313
BglII A'GATC_T
Cuts at: 0 1413 2076
Size: 1413 663
Bme1580I G_kGCm'C
Cuts at: 0 78 2076
Size: 78 1998
BmrI ACTGGGnnnn_n'
Cuts at: 0 1123 1455 1608 2076
Size: 1123 332 153 468
Fragments arranged by size:
1123 468 332 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 1874 1952 2076
Size: 941 933 78 124
Fragments arranged by size:
941 933 124 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1005 2076
Size: 1005 1071
BsaBI GATnn'nnATC
Cuts at: 0 1169 2076
Size: 1169 907
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 662 692 1087 1117 1582 1612
Size: 190 30 442 30 395 30 465 30
Cuts at: 1612 2076
Size: 464
Fragments arranged by size:
465 464 442 395 190 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 553 948 1171 1587 2076
Size: 553 395 223 416 489
Fragments arranged by size:
553 489 416 395 223
BseYI C'CCAG_C
Cuts at: 0 1395 1649 2076
Size: 1395 254 427
Fragments arranged by size:
1395 427 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 433 2076
Size: 177 256 1643
Fragments arranged by size:
1643 256 177
BsiHKAI G_wGCw'C
Cuts at: 0 1330 1942 2076
Size: 1330 612 134
Fragments arranged by size:
1330 612 134
Bsp1286I G_dGCh'C
Cuts at: 0 78 132 217 592 1330 1423 1942 2076
Size: 78 54 85 375 738 93 519 134
Fragments arranged by size:
738 519 375 134 93 85 78 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1442 2076
Size: 1442 634
BsrFI r'CCGG_y
Cuts at: 0 767 1076 2076
Size: 767 309 1000
Fragments arranged by size:
1000 767 309
BsrGI T'GTAC_A
Cuts at: 0 395 2076
Size: 395 1681
BstYI r'GATC_y
Cuts at: 0 719 755 1413 1543 2076
Size: 719 36 658 130 533
Fragments arranged by size:
719 658 533 130 36
Bsu36I CC'TnA_GG
Cuts at: 0 70 1238 2076
Size: 70 1168 838
Fragments arranged by size:
1168 838 70
BtsI GCAGTG_nn'
Cuts at: 0 96 1402 1881 2076
Size: 96 1306 479 195
Fragments arranged by size:
1306 479 195 96
DraI TTT'AAA
Cuts at: 0 1479 2076
Size: 1479 597
DraIII CAC_nnn'GTG
Cuts at: 0 875 2076
Size: 875 1201
EaeI y'GGCC_r
Cuts at: 0 23 2076
Size: 23 2053
EarI CTCTTCn'nnn_
Cuts at: 0 305 1120 1947 2076
Size: 305 815 827 129
Fragments arranged by size:
827 815 305 129
EcoICRI GAG'CTC
Cuts at: 0 1328 1940 2076
Size: 1328 612 136
Fragments arranged by size:
1328 612 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 941 1144 1874 1952 2076
Size: 393 548 203 730 78 124
Fragments arranged by size:
730 548 393 203 124 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1438 2076
Size: 1438 638
EcoO109I rG'GnC_Cy
Cuts at: 0 74 2076
Size: 74 2002
EcoRI G'AATT_C
Cuts at: 0 836 1605 2076
Size: 836 769 471
Fragments arranged by size:
836 769 471
EcoRV GAT'ATC
Cuts at: 0 278 2076
Size: 278 1798
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 280 312 711 743 1405 1437 1567 1599
Size: 280 32 399 32 662 32 130 32
Cuts at: 1599 1645 1677 1727 1759 1789 1821 2076
Size: 46 32 50 32 30 32 255
Fragments arranged by size:
662 399 280 255 130 50 46 32
32 32 32 32 32 32 30
HincII GTy'rAC
Cuts at: 0 209 1619 2076
Size: 209 1410 457
Fragments arranged by size:
1410 457 209
HindIII A'AGCT_T
Cuts at: 0 1558 2076
Size: 1558 518
HpaI GTT'AAC
Cuts at: 0 209 2076
Size: 209 1867
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 131 1388 2076
Size: 58 73 1257 688
Fragments arranged by size:
1257 688 73 58
MslI CAynn'nnrTG
Cuts at: 0 1039 2076
Size: 1039 1037
MspA1I CmG'CkG
Cuts at: 0 337 2076
Size: 337 1739
NaeI GCC'GGC
Cuts at: 0 769 2076
Size: 769 1307
NgoMIV G'CCGG_C
Cuts at: 0 767 2076
Size: 767 1309
NsiI A_TGCA'T
Cuts at: 0 765 2076
Size: 765 1311
NspI r_CATG'y
Cuts at: 0 702 714 767 2076
Size: 702 12 53 1309
Fragments arranged by size:
1309 702 53 12
PciI A'CATG_T
Cuts at: 0 698 2076
Size: 698 1378
PflMI CCAn_nnn'nTGG
Cuts at: 0 875 2076
Size: 875 1201
PfoI T'CCnGG_A
Cuts at: 0 348 2076
Size: 348 1728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 662 694 2076
Size: 662 32 1382
Fragments arranged by size:
1382 662 32
PspOMI G'GGCC_C
Cuts at: 0 74 2076
Size: 74 2002
PstI C_TGCA'G
Cuts at: 0 1260 1309 2076
Size: 1260 49 767
Fragments arranged by size:
1260 767 49
PvuII CAG'CTG
Cuts at: 0 337 2076
Size: 337 1739
SacI G_AGCT'C
Cuts at: 0 1330 1942 2076
Size: 1330 612 134
Fragments arranged by size:
1330 612 134
SapI GCTCTTCn'nnn_
Cuts at: 0 1947 2076
Size: 1947 129
ScaI AGT'ACT
Cuts at: 0 1851 2076
Size: 1851 225
SfcI C'TryA_G
Cuts at: 0 963 1256 1305 1731 2076
Size: 963 293 49 426 345
Fragments arranged by size:
963 426 345 293 49
SmlI C'TyrA_G
Cuts at: 0 984 2076
Size: 984 1092
SphI G_CATG'C
Cuts at: 0 767 2076
Size: 767 1309
SspI AAT'ATT
Cuts at: 0 640 790 1755 2005 2076
Size: 640 150 965 250 71
Fragments arranged by size:
965 640 250 150 71
StyI C'CwwG_G
Cuts at: 0 776 1715 2076
Size: 776 939 361
Fragments arranged by size:
939 776 361
TatI w'GTAC_w
Cuts at: 0 395 1425 1849 2076
Size: 395 1030 424 227
Fragments arranged by size:
1030 424 395 227
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1582 1614 2076
Size: 1582 32 462
Fragments arranged by size:
1582 462 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 872 2076
Size: 872 1204
XmnI GAAnn'nnTTC
Cuts at: 0 791 822 1229 2076
Size: 791 31 407 847
Fragments arranged by size:
847 791 407 31
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApaI ApoI AvaI
BaeI BanII BbsI BcgI BciVI BfrBI BglII Bme1580I
BmrI BpmI BpuEI BsaBI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BsrGI BstYI Bsu36I BtsI
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV Hin4I HincII HindIII HpaI MmeI MslI
MspA1I NaeI NgoMIV NsiI NspI PciI PflMI PfoI
PpiI PspOMI PstI PvuII SacI SapI ScaI SfcI
SmlI SphI SspI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvrII BamHI
BanI BbeI BbvCI BclI BglI BlpI BmgBI BmtI
BplI Bpu10I BsaI BsaAI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI DrdI EagI EciI FalI FseI FspI FspAI
HaeII KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI PacI PasI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI