Restriction map for
pF1KB9971
(Linear) (Six-base) MAPSORT of: KIBB9971.seq Check: 2043 from: 1 to: 1662
KIBB9971.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 353 1662
Size: 353 1309
AccI GT'mk_AC
Cuts at: 0 308 1662
Size: 308 1354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1437 1662
Size: 908 529 225
Fragments arranged by size:
908 529 225
AflII C'TTAA_G
Cuts at: 0 1630 1662
Size: 1630 32
AflIII A'CryG_T
Cuts at: 0 596 1662
Size: 596 1066
AlwNI CAG_nnn'CTG
Cuts at: 0 630 1662
Size: 630 1032
ApaI G_GGCC'C
Cuts at: 0 236 1662
Size: 236 1426
ApoI r'AATT_y
Cuts at: 0 676 1227 1662
Size: 676 551 435
Fragments arranged by size:
676 551 435
AvaI C'yCGr_G
Cuts at: 0 687 1161 1653 1662
Size: 687 474 492 9
Fragments arranged by size:
687 492 474 9
BanI G'GyrC_C
Cuts at: 0 1105 1657 1662
Size: 1105 552 5
Fragments arranged by size:
1105 552 5
BanII G_rGCy'C
Cuts at: 0 196 236 694 994 1091 1662
Size: 196 40 458 300 97 571
Fragments arranged by size:
571 458 300 196 97 40
BbeI G_GCGC'C
Cuts at: 0 1109 1662
Size: 1109 553
BbsI GAAGACnn'nnnn_
Cuts at: 0 111 1443 1662
Size: 111 1332 219
Fragments arranged by size:
1332 219 111
BbvCI CC'TCA_GC
Cuts at: 0 298 1459 1662
Size: 298 1161 203
Fragments arranged by size:
1161 298 203
BglI GCCn_nnn'nGGC
Cuts at: 0 368 780 1662
Size: 368 412 882
Fragments arranged by size:
882 412 368
BlpI GC'TnA_GC
Cuts at: 0 167 172 988 1361 1662
Size: 167 5 816 373 301
Fragments arranged by size:
816 373 301 167 5
Bme1580I G_kGCm'C
Cuts at: 0 236 1660 1662
Size: 236 1424 2
Fragments arranged by size:
1424 236 2
BmtI G_CTAG'C
Cuts at: 0 323 1662
Size: 323 1339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 210 678 1662
Size: 43 167 468 984
Fragments arranged by size:
984 468 167 43
Bpu10I CC'TnA_GC
Cuts at: 0 298 1459 1662
Size: 298 1161 203
Fragments arranged by size:
1161 298 203
BsaI GGTCTCn'nnnn_
Cuts at: 0 1537 1561 1662
Size: 1537 24 101
Fragments arranged by size:
1537 101 24
BsaBI GATnn'nnATC
Cuts at: 0 1264 1662
Size: 1264 398
BsaHI Gr'CG_yC
Cuts at: 0 1106 1580 1662
Size: 1106 474 82
Fragments arranged by size:
1106 474 82
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1385 1415 1662
Size: 1385 30 247
Fragments arranged by size:
1385 247 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 1662
Size: 217 1445
BseYI C'CCAG_C
Cuts at: 0 351 371 1662
Size: 351 20 1291
Fragments arranged by size:
1291 351 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1081 1264 1662
Size: 282 799 183 398
Fragments arranged by size:
799 398 282 183
BsiHKAI G_wGCw'C
Cuts at: 0 575 1079 1662
Size: 575 504 583
Fragments arranged by size:
583 575 504
BsmI GAATG_Cn'
Cuts at: 0 471 1362 1662
Size: 471 891 300
Fragments arranged by size:
891 471 300
Bsp1286I G_dGCh'C
Cuts at: 0 196 236 575 694 994 1079 1091 1660
Size: 196 40 339 119 300 85 12 569
Cuts at: 1660 1662
Size: 2
Fragments arranged by size:
569 339 300 196 119 85 40 12
2
BspHI T'CATG_A
Cuts at: 0 1256 1662
Size: 1256 406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 18 353 1286 1662
Size: 18 335 933 376
Fragments arranged by size:
933 376 335 18
BsrBI CCG'CTC
Cuts at: 0 1147 1662
Size: 1147 515
BsrDI GCAATG_nn'
Cuts at: 0 1328 1662
Size: 1328 334
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1479 1662
Size: 1479 183
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 157 184 1662
Size: 108 49 27 1478
Fragments arranged by size:
1478 108 49 27
Bsu36I CC'TnA_GG
Cuts at: 0 1027 1662
Size: 1027 635
BtgI C'CryG_G
Cuts at: 0 8 236 1214 1517 1662
Size: 8 228 978 303 145
Fragments arranged by size:
978 303 228 145 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 897 1662
Size: 897 765
BtsI GCAGTG_nn'
Cuts at: 0 144 1662
Size: 144 1518
DraI TTT'AAA
Cuts at: 0 950 1662
Size: 950 712
EaeI y'GGCC_r
Cuts at: 0 255 326 519 1217 1311 1662
Size: 255 71 193 698 94 351
Fragments arranged by size:
698 351 255 193 94 71
EarI CTCTTCn'nnn_
Cuts at: 0 399 616 826 962 1662
Size: 399 217 210 136 700
Fragments arranged by size:
700 399 217 210 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 604 1662
Size: 604 1058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 210 678 908 1437 1662
Size: 43 167 468 230 529 225
Fragments arranged by size:
529 468 230 225 167 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 622 967 1662
Size: 622 345 695
Fragments arranged by size:
695 622 345
EcoO109I rG'GnC_Cy
Cuts at: 0 232 355 627 1166 1662
Size: 232 123 272 539 496
Fragments arranged by size:
539 496 272 232 123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 78 1056 1662
Size: 78 978 606
Fragments arranged by size:
978 606 78
HaeII r_GCGC'y
Cuts at: 0 270 296 751 786 1109 1662
Size: 270 26 455 35 323 553
Fragments arranged by size:
553 455 323 270 35 26
HincII GTy'rAC
Cuts at: 0 555 1662
Size: 555 1107
KasI G'GCGC_C
Cuts at: 0 1105 1662
Size: 1105 557
MscI TGG'CCA
Cuts at: 0 257 328 1219 1662
Size: 257 71 891 443
Fragments arranged by size:
891 443 257 71
MslI CAynn'nnrTG
Cuts at: 0 106 601 1048 1219 1300 1662
Size: 106 495 447 171 81 362
Fragments arranged by size:
495 447 362 171 106 81
MspA1I CmG'CkG
Cuts at: 0 238 302 351 795 1115 1519 1662
Size: 238 64 49 444 320 404 143
Fragments arranged by size:
444 404 320 238 143 64 49
NarI GG'CG_CC
Cuts at: 0 1106 1662
Size: 1106 556
NcoI C'CATG_G
Cuts at: 0 1214 1662
Size: 1214 448
NheI G'CTAG_C
Cuts at: 0 319 1662
Size: 319 1343
NspI r_CATG'y
Cuts at: 0 600 1662
Size: 600 1062
PciI A'CATG_T
Cuts at: 0 596 1662
Size: 596 1066
PfoI T'CCnGG_A
Cuts at: 0 433 1662
Size: 433 1229
PpuMI rG'GwC_Cy
Cuts at: 0 355 1662
Size: 355 1307
PspOMI G'GGCC_C
Cuts at: 0 232 1662
Size: 232 1430
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1662
Size: 1045 32 585
Fragments arranged by size:
1045 585 32
PstI C_TGCA'G
Cuts at: 0 626 794 1662
Size: 626 168 868
Fragments arranged by size:
868 626 168
PvuII CAG'CTG
Cuts at: 0 302 795 1662
Size: 302 493 867
Fragments arranged by size:
867 493 302
SacII CC_GC'GG
Cuts at: 0 239 1520 1662
Size: 239 1281 142
Fragments arranged by size:
1281 239 142
SanDI GG'GwC_CC
Cuts at: 0 355 1662
Size: 355 1307
SapI GCTCTTCn'nnn_
Cuts at: 0 399 616 1662
Size: 399 217 1046
Fragments arranged by size:
1046 399 217
SexAI A'CCwGG_T
Cuts at: 0 416 1662
Size: 416 1246
SfcI C'TryA_G
Cuts at: 0 622 790 1662
Size: 622 168 872
Fragments arranged by size:
872 622 168
SfoI GGC'GCC
Cuts at: 0 1107 1662
Size: 1107 555
SmaI CCC'GGG
Cuts at: 0 1163 1662
Size: 1163 499
SmlI C'TyrA_G
Cuts at: 0 1630 1662
Size: 1630 32
StuI AGG'CCT
Cuts at: 0 227 247 1662
Size: 227 20 1415
Fragments arranged by size:
1415 227 20
StyI C'CwwG_G
Cuts at: 0 252 1214 1547 1590 1662
Size: 252 962 333 43 72
Fragments arranged by size:
962 333 252 72 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 363 1662
Size: 363 1299
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1385 1417 1662
Size: 1385 32 245
Fragments arranged by size:
1385 245 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 702 1194 1662
Size: 702 492 468
Fragments arranged by size:
702 492 468
XmaI C'CCGG_G
Cuts at: 0 1161 1662
Size: 1161 501
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BglI BlpI
Bme1580I BmtI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BstAPI BstXI Bsu36I BtgI BtgZI BtsI
DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI
HaeII HincII KasI MscI MslI MspA1I NarI NcoI
NheI NspI PciI PfoI PpuMI PspOMI PsrI PstI
PvuII SacII SanDI SapI SexAI SfcI SfoI SmaI
SmlI StuI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BplI
BpuEI BsaAI BsaWI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I ClaI
CspCI DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
MmeI NaeI NdeI NgoMIV NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI RsrII SacI SalI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmnI ZraI