Restriction map for
pF1KB9972
(Linear) (Six-base) MAPSORT of: KIBB9972.seq Check: 5362 from: 1 to: 2112
KIBB9972.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AleI CACnn'nnGTG
Cuts at: 0 1616 1786 2112
Size: 1616 170 326
Fragments arranged by size:
1616 326 170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 458 2112
Size: 458 1654
AlwNI CAG_nnn'CTG
Cuts at: 0 1263 1878 1911 2001 2112
Size: 1263 615 33 90 111
Fragments arranged by size:
1263 615 111 90 33
ApaI G_GGCC'C
Cuts at: 0 133 2112
Size: 133 1979
ApoI r'AATT_y
Cuts at: 0 1270 2112
Size: 1270 842
AvaI C'yCGr_G
Cuts at: 0 669 803 2112
Size: 669 134 1309
Fragments arranged by size:
1309 669 134
AvrII C'CTAG_G
Cuts at: 0 2056 2112
Size: 2056 56
BanI G'GyrC_C
Cuts at: 0 60 454 1477 2112
Size: 60 394 1023 635
Fragments arranged by size:
1023 635 394 60
BanII G_rGCy'C
Cuts at: 0 133 1081 1601 1745 2112
Size: 133 948 520 144 367
Fragments arranged by size:
948 520 367 144 133
BbeI G_GCGC'C
Cuts at: 0 64 2112
Size: 64 2048
BbvCI CC'TCA_GC
Cuts at: 0 572 1198 2112
Size: 572 626 914
Fragments arranged by size:
914 626 572
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 573 607 2112
Size: 573 34 1505
Fragments arranged by size:
1505 573 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 465 2112
Size: 465 1647
BclI T'GATC_A
Cuts at: 0 1265 2112
Size: 1265 847
BglI GCCn_nnn'nGGC
Cuts at: 0 1146 1431 2112
Size: 1146 285 681
Fragments arranged by size:
1146 681 285
BlpI GC'TnA_GC
Cuts at: 0 1994 2112
Size: 1994 118
Bme1580I G_kGCm'C
Cuts at: 0 133 1037 2112
Size: 133 904 1075
Fragments arranged by size:
1075 904 133
BmrI ACTGGGnnnn_n'
Cuts at: 0 117 2112
Size: 117 1995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 366 1095 2112
Size: 366 729 1017
Fragments arranged by size:
1017 729 366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 728 1065 1858 1945 2112
Size: 171 557 337 793 87 167
Fragments arranged by size:
793 557 337 171 167 87
Bpu10I CC'TnA_GC
Cuts at: 0 572 1198 2112
Size: 572 626 914
Fragments arranged by size:
914 626 572
BsaAI yAC'GTr
Cuts at: 0 1032 2112
Size: 1032 1080
BsaHI Gr'CG_yC
Cuts at: 0 61 2112
Size: 61 2051
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 666 696 1672 1702 2112
Size: 666 30 976 30 410
Fragments arranged by size:
976 666 410 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 949 1696 1753 1909 2112
Size: 949 747 57 156 203
Fragments arranged by size:
949 747 203 156 57
BseYI C'CCAG_C
Cuts at: 0 144 1074 1704 1767 2112
Size: 144 930 630 63 345
Fragments arranged by size:
930 630 345 144 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 262 970 1594 2112
Size: 196 66 708 624 518
Fragments arranged by size:
708 624 518 196 66
BsiEI CG_ry'CG
Cuts at: 0 77 2112
Size: 77 2035
BsiHKAI G_wGCw'C
Cuts at: 0 1529 1601 1745 2112
Size: 1529 72 144 367
Fragments arranged by size:
1529 367 144 72
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1645 1726 2112
Size: 1645 81 386
Fragments arranged by size:
1645 386 81
Bsp1286I G_dGCh'C
Cuts at: 0 133 1037 1081 1529 1601 1745 2112
Size: 133 904 44 448 72 144 367
Fragments arranged by size:
904 448 367 144 133 72 44
BsrBI CCG'CTC
Cuts at: 0 659 1008 2112
Size: 659 349 1104
Fragments arranged by size:
1104 659 349
BsrFI r'CCGG_y
Cuts at: 0 63 1474 2112
Size: 63 1411 638
Fragments arranged by size:
1411 638 63
BsrGI T'GTAC_A
Cuts at: 0 1094 2112
Size: 1094 1018
BssHII G'CGCG_C
Cuts at: 0 4 1899 1971 2112
Size: 4 1895 72 141
Fragments arranged by size:
1895 141 72 4
BstEII G'GTnAC_C
Cuts at: 0 682 1794 1827 2112
Size: 682 1112 33 285
Fragments arranged by size:
1112 682 285 33
BstXI CCAn_nnnn'nTGG
Cuts at: 0 475 2112
Size: 475 1637
BtgI C'CryG_G
Cuts at: 0 1127 1823 2063 2112
Size: 1127 696 240 49
Fragments arranged by size:
1127 696 240 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 377 2112
Size: 377 1735
BtsI GCAGTG_nn'
Cuts at: 0 409 2112
Size: 409 1703
EaeI y'GGCC_r
Cuts at: 0 74 402 594 1880 2112
Size: 74 328 192 1286 232
Fragments arranged by size:
1286 328 232 192 74
EagI C'GGCC_G
Cuts at: 0 74 2112
Size: 74 2038
EarI CTCTTCn'nnn_
Cuts at: 0 491 1579 2112
Size: 491 1088 533
Fragments arranged by size:
1088 533 491
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 885 2112
Size: 885 1227
EcoICRI GAG'CTC
Cuts at: 0 1599 1743 2112
Size: 1599 144 369
Fragments arranged by size:
1599 369 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 728 1065 1858 1945 2112
Size: 171 557 337 793 87 167
Fragments arranged by size:
793 557 337 171 167 87
EcoO109I rG'GnC_Cy
Cuts at: 0 130 1700 2097 2112
Size: 130 1570 397 15
Fragments arranged by size:
1570 397 130 15
FspI TGC'GCA
Cuts at: 0 1867 2112
Size: 1867 245
HaeII r_GCGC'y
Cuts at: 0 22 64 70 1423 2112
Size: 22 42 6 1353 689
Fragments arranged by size:
1353 689 42 22 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 632 664 1684 1705 1716 1737
Size: 490 32 110 32 1020 21 11 21
Cuts at: 1737 2112
Size: 375
Fragments arranged by size:
1020 490 375 110 32 32 21 21
11
HindIII A'AGCT_T
Cuts at: 0 286 728 2112
Size: 286 442 1384
Fragments arranged by size:
1384 442 286
KasI G'GCGC_C
Cuts at: 0 60 2112
Size: 60 2052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 800 1827 2112
Size: 533 267 1027 285
Fragments arranged by size:
1027 533 285 267
MscI TGG'CCA
Cuts at: 0 1882 2112
Size: 1882 230
MslI CAynn'nnrTG
Cuts at: 0 895 1616 1663 1786 2112
Size: 895 721 47 123 326
Fragments arranged by size:
895 721 326 123 47
MspA1I CmG'CkG
Cuts at: 0 1878 1968 1998 2112
Size: 1878 90 30 114
Fragments arranged by size:
1878 114 90 30
NaeI GCC'GGC
Cuts at: 0 65 1476 2112
Size: 65 1411 636
Fragments arranged by size:
1411 636 65
NarI GG'CG_CC
Cuts at: 0 61 2112
Size: 61 2051
NcoI C'CATG_G
Cuts at: 0 1823 2063 2112
Size: 1823 240 49
Fragments arranged by size:
1823 240 49
NgoMIV G'CCGG_C
Cuts at: 0 63 1474 2112
Size: 63 1411 638
Fragments arranged by size:
1411 638 63
NspI r_CATG'y
Cuts at: 0 1470 1872 2112
Size: 1470 402 240
Fragments arranged by size:
1470 402 240
PfoI T'CCnGG_A
Cuts at: 0 467 548 1241 2112
Size: 467 81 693 871
Fragments arranged by size:
871 693 467 81
PpuMI rG'GwC_Cy
Cuts at: 0 1700 2112
Size: 1700 412
PspOMI G'GGCC_C
Cuts at: 0 129 2112
Size: 129 1983
PstI C_TGCA'G
Cuts at: 0 563 1217 1916 2112
Size: 563 654 699 196
Fragments arranged by size:
699 654 563 196
PvuII CAG'CTG
Cuts at: 0 1878 1968 1998 2112
Size: 1878 90 30 114
Fragments arranged by size:
1878 114 90 30
SacI G_AGCT'C
Cuts at: 0 1601 1745 2112
Size: 1601 144 367
Fragments arranged by size:
1601 367 144
SapI GCTCTTCn'nnn_
Cuts at: 0 491 2112
Size: 491 1621
SfcI C'TryA_G
Cuts at: 0 559 1213 1912 2112
Size: 559 654 699 200
Fragments arranged by size:
699 654 559 200
SfoI GGC'GCC
Cuts at: 0 62 2112
Size: 62 2050
SgrAI Cr'CCGG_yG
Cuts at: 0 63 2112
Size: 63 2049
SmaI CCC'GGG
Cuts at: 0 671 805 2112
Size: 671 134 1307
Fragments arranged by size:
1307 671 134
SphI G_CATG'C
Cuts at: 0 1470 1872 2112
Size: 1470 402 240
Fragments arranged by size:
1470 402 240
StuI AGG'CCT
Cuts at: 0 544 2112
Size: 544 1568
StyI C'CwwG_G
Cuts at: 0 368 908 1121 1823 2056 2063 2112
Size: 368 540 213 702 233 7 49
Fragments arranged by size:
702 540 368 233 213 49 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1828 2112
Size: 1828 284
TatI w'GTAC_w
Cuts at: 0 314 827 854 1094 2112
Size: 314 513 27 240 1018
Fragments arranged by size:
1018 513 314 240 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 757 789 2112
Size: 757 32 1323
Fragments arranged by size:
1323 757 32
Tth111I GACn'n_nGTC
Cuts at: 0 1660 2112
Size: 1660 452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 366 897 1515 2112
Size: 123 243 531 618 597
Fragments arranged by size:
618 597 531 243 123
XmaI C'CCGG_G
Cuts at: 0 669 803 2112
Size: 669 134 1309
Fragments arranged by size:
1309 669 134
Enzymes that do cut:
AleI AlfI AlwNI ApaI ApoI AvaI AvrII BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI
BsrFI BsrGI BssHII BstEII BstXI BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PfoI PpuMI PspOMI
PstI PvuII SacI SapI SfcI SfoI SgrAI SmaI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI
BaeI BamHI BbsI BfrBI BglII BmgBI BmtI BpuEI
BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI BspMI
BsrDI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI
FseI FspAI HincII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SmlI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI XmnI ZraI