Restriction map for
pF1KB9973
(Linear) (Six-base) MAPSORT of: KIBB9973.seq Check: 9355 from: 1 to: 1662
KIBB9973.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 149 778 1505 1662
Size: 149 629 727 157
Fragments arranged by size:
727 629 157 149
AccI GT'mk_AC
Cuts at: 0 209 1662
Size: 209 1453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 376 1662
Size: 279 97 1286
Fragments arranged by size:
1286 279 97
AflII C'TTAA_G
Cuts at: 0 244 1662
Size: 244 1418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1318 1662
Size: 1318 344
AlwNI CAG_nnn'CTG
Cuts at: 0 150 159 435 720 1662
Size: 150 9 276 285 942
Fragments arranged by size:
942 285 276 150 9
ApaI G_GGCC'C
Cuts at: 0 1114 1662
Size: 1114 548
AvaI C'yCGr_G
Cuts at: 0 865 1290 1662
Size: 865 425 372
Fragments arranged by size:
865 425 372
BamHI G'GATC_C
Cuts at: 0 732 1459 1662
Size: 732 727 203
Fragments arranged by size:
732 727 203
BanI G'GyrC_C
Cuts at: 0 13 1503 1662
Size: 13 1490 159
Fragments arranged by size:
1490 159 13
BanII G_rGCy'C
Cuts at: 0 52 1010 1114 1354 1662
Size: 52 958 104 240 308
Fragments arranged by size:
958 308 240 104 52
BbeI G_GCGC'C
Cuts at: 0 17 1662
Size: 17 1645
BbsI GAAGACnn'nnnn_
Cuts at: 0 1162 1482 1662
Size: 1162 320 180
Fragments arranged by size:
1162 320 180
BbvCI CC'TCA_GC
Cuts at: 0 1237 1662
Size: 1237 425
BglI GCCn_nnn'nGGC
Cuts at: 0 652 1232 1662
Size: 652 580 430
Fragments arranged by size:
652 580 430
BlpI GC'TnA_GC
Cuts at: 0 1262 1662
Size: 1262 400
Bme1580I G_kGCm'C
Cuts at: 0 901 1114 1508 1662
Size: 901 213 394 154
Fragments arranged by size:
901 394 213 154
BmrI ACTGGGnnnn_n'
Cuts at: 0 793 1502 1662
Size: 793 709 160
Fragments arranged by size:
793 709 160
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 711 1218 1479 1662
Size: 711 507 261 183
Fragments arranged by size:
711 507 261 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 457 519 947 960 1339 1449 1462
Size: 412 45 62 428 13 379 110 13
Cuts at: 1462 1662
Size: 200
Fragments arranged by size:
428 412 379 200 110 62 45 13
13
Bpu10I CC'TnA_GC
Cuts at: 0 1237 1662
Size: 1237 425
BsaI GGTCTCn'nnnn_
Cuts at: 0 172 787 1178 1662
Size: 172 615 391 484
Fragments arranged by size:
615 484 391 172
BsaHI Gr'CG_yC
Cuts at: 0 14 1662
Size: 14 1648
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 1662
Size: 169 30 1463
Fragments arranged by size:
1463 169 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 1278 1344 1362 1662
Size: 112 1166 66 18 300
Fragments arranged by size:
1166 300 112 66 18
BseYI C'CCAG_C
Cuts at: 0 45 1018 1662
Size: 45 973 644
Fragments arranged by size:
973 644 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 706 1662
Size: 349 357 956
Fragments arranged by size:
956 357 349
BsiHKAI G_wGCw'C
Cuts at: 0 769 1336 1662
Size: 769 567 326
Fragments arranged by size:
769 567 326
BsmI GAATG_Cn'
Cuts at: 0 535 1662
Size: 535 1127
Bsp1286I G_dGCh'C
Cuts at: 0 52 769 901 1010 1114 1336 1354 1508
Size: 52 717 132 109 104 222 18 154
Cuts at: 1508 1662
Size: 154
Fragments arranged by size:
717 222 154 154 132 109 104 52
18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 778 1505 1662
Size: 149 629 727 157
Fragments arranged by size:
727 629 157 149
BsrBI CCG'CTC
Cuts at: 0 1591 1662
Size: 1591 71
BsrDI GCAATG_nn'
Cuts at: 0 899 1662
Size: 899 763
BsrFI r'CCGG_y
Cuts at: 0 1074 1662
Size: 1074 588
BssSI C'ACGA_G
Cuts at: 0 183 1662
Size: 183 1479
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 509 720 1662
Size: 150 359 211 942
Fragments arranged by size:
942 359 211 150
BstEII G'GTnAC_C
Cuts at: 0 549 1662
Size: 549 1113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 127 1662
Size: 127 1535
BstYI r'GATC_y
Cuts at: 0 228 732 1211 1459 1662
Size: 228 504 479 248 203
Fragments arranged by size:
504 479 248 228 203
BtgI C'CryG_G
Cuts at: 0 641 1662
Size: 641 1021
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 303 1662
Size: 303 1359
BtsI GCAGTG_nn'
Cuts at: 0 603 747 1219 1662
Size: 603 144 472 443
Fragments arranged by size:
603 472 443 144
DraIII CAC_nnn'GTG
Cuts at: 0 729 1662
Size: 729 933
EaeI y'GGCC_r
Cuts at: 0 754 1128 1529 1662
Size: 754 374 401 133
Fragments arranged by size:
754 401 374 133
EarI CTCTTCn'nnn_
Cuts at: 0 352 377 1662
Size: 352 25 1285
Fragments arranged by size:
1285 352 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1581 1662
Size: 1581 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 376 412 457 519 947 960 1339
Size: 279 97 36 45 62 428 13 379
Cuts at: 1339 1449 1462 1662
Size: 110 13 200
Fragments arranged by size:
428 379 279 200 110 97 62 45
36 13 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 481 1101 1630 1662
Size: 247 234 620 529 32
Fragments arranged by size:
620 529 247 234 32
EcoO109I rG'GnC_Cy
Cuts at: 0 854 909 1111 1233 1397 1662
Size: 854 55 202 122 164 265
Fragments arranged by size:
854 265 202 164 122 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 381 471 1662
Size: 381 90 1191
Fragments arranged by size:
1191 381 90
HaeII r_GCGC'y
Cuts at: 0 17 27 1662
Size: 17 10 1635
Fragments arranged by size:
1635 17 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 153 185 592 624 1149 1153
Size: 88 32 33 32 407 32 525 4
Cuts at: 1153 1181 1185 1435 1467 1662
Size: 28 4 250 32 195
Fragments arranged by size:
525 407 250 195 88 33 32 32
32 32 28 4 4
KasI G'GCGC_C
Cuts at: 0 13 1662
Size: 13 1649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 1515 1662
Size: 86 1429 147
Fragments arranged by size:
1429 147 86
MscI TGG'CCA
Cuts at: 0 1130 1662
Size: 1130 532
MspA1I CmG'CkG
Cuts at: 0 156 333 753 1662
Size: 156 177 420 909
Fragments arranged by size:
909 420 177 156
NaeI GCC'GGC
Cuts at: 0 1076 1662
Size: 1076 586
NarI GG'CG_CC
Cuts at: 0 14 1662
Size: 14 1648
NcoI C'CATG_G
Cuts at: 0 641 1662
Size: 641 1021
NgoMIV G'CCGG_C
Cuts at: 0 1074 1662
Size: 1074 588
NspI r_CATG'y
Cuts at: 0 999 1662
Size: 999 663
PasI CC'CwG_GG
Cuts at: 0 1103 1230 1662
Size: 1103 127 432
Fragments arranged by size:
1103 432 127
PflMI CCAn_nnn'nTGG
Cuts at: 0 41 435 546 1500 1662
Size: 41 394 111 954 162
Fragments arranged by size:
954 394 162 111 41
PfoI T'CCnGG_A
Cuts at: 0 1478 1662
Size: 1478 184
PpuMI rG'GwC_Cy
Cuts at: 0 909 1662
Size: 909 753
PspOMI G'GGCC_C
Cuts at: 0 1110 1662
Size: 1110 552
PstI C_TGCA'G
Cuts at: 0 146 155 338 1662
Size: 146 9 183 1324
Fragments arranged by size:
1324 183 146 9
PvuII CAG'CTG
Cuts at: 0 333 1662
Size: 333 1329
SanDI GG'GwC_CC
Cuts at: 0 909 1662
Size: 909 753
SapI GCTCTTCn'nnn_
Cuts at: 0 352 377 1662
Size: 352 25 1285
Fragments arranged by size:
1285 352 25
ScaI AGT'ACT
Cuts at: 0 112 1662
Size: 112 1550
SfcI C'TryA_G
Cuts at: 0 114 142 151 334 1551 1662
Size: 114 28 9 183 1217 111
Fragments arranged by size:
1217 183 114 111 28 9
SfoI GGC'GCC
Cuts at: 0 15 1662
Size: 15 1647
SmaI CCC'GGG
Cuts at: 0 867 1662
Size: 867 795
SmlI C'TyrA_G
Cuts at: 0 244 1662
Size: 244 1418
SphI G_CATG'C
Cuts at: 0 999 1662
Size: 999 663
SspI AAT'ATT
Cuts at: 0 495 1662
Size: 495 1167
StuI AGG'CCT
Cuts at: 0 397 1541 1662
Size: 397 1144 121
Fragments arranged by size:
1144 397 121
StyI C'CwwG_G
Cuts at: 0 371 641 954 1308 1601 1662
Size: 371 270 313 354 293 61
Fragments arranged by size:
371 354 313 293 270 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 774 1662
Size: 774 888
TatI w'GTAC_w
Cuts at: 0 110 1662
Size: 110 1552
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 169 201 717 749 1662
Size: 169 32 516 32 913
Fragments arranged by size:
913 516 169 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 42 543 648 1515 1662
Size: 42 501 105 867 147
Fragments arranged by size:
867 501 147 105 42
XmaI C'CCGG_G
Cuts at: 0 865 1662
Size: 865 797
XmnI GAAnn'nnTTC
Cuts at: 0 496 1662
Size: 496 1166
Enzymes that do cut:
AarI AccI AcuI AflII AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SanDI SapI ScaI SfcI
SfoI SmaI SmlI SphI SspI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BpuEI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI
BsrGI BssHII BstBI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MslI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SwaI Tth111I XbaI XhoI ZraI