Restriction map for
pF1KB9977
(Linear) (Six-base) MAPSORT of: KIBB9977.seq Check: 6933 from: 1 to: 1671
KIBB9977.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 64 1671
Size: 64 1607
AccI GT'mk_AC
Cuts at: 0 113 1671
Size: 113 1558
AclI AA'CG_TT
Cuts at: 0 887 1671
Size: 887 784
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 503 977 1671
Size: 431 72 474 694
Fragments arranged by size:
694 474 431 72
AleI CACnn'nnGTG
Cuts at: 0 1078 1671
Size: 1078 593
ApoI r'AATT_y
Cuts at: 0 563 902 1307 1325 1671
Size: 563 339 405 18 346
Fragments arranged by size:
563 405 346 339 18
BanI G'GyrC_C
Cuts at: 0 48 1671
Size: 48 1623
BbeI G_GCGC'C
Cuts at: 0 52 1671
Size: 52 1619
BbsI GAAGACnn'nnnn_
Cuts at: 0 1368 1392 1671
Size: 1368 24 279
Fragments arranged by size:
1368 279 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 15 49 1671
Size: 15 34 1622
Fragments arranged by size:
1622 34 15
BfrBI ATG'CAT
Cuts at: 0 1388 1671
Size: 1388 283
BmrI ACTGGGnnnn_n'
Cuts at: 0 212 861 1671
Size: 212 649 810
Fragments arranged by size:
810 649 212
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 1461 1671
Size: 369 1092 210
Fragments arranged by size:
1092 369 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 986 1671
Size: 374 612 685
Fragments arranged by size:
685 612 374
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1401 1671
Size: 259 1142 270
Fragments arranged by size:
1142 270 259
BsaBI GATnn'nnATC
Cuts at: 0 433 1565 1671
Size: 433 1132 106
Fragments arranged by size:
1132 433 106
BsaHI Gr'CG_yC
Cuts at: 0 49 1671
Size: 49 1622
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 86 1671
Size: 86 1585
BsiHKAI G_wGCw'C
Cuts at: 0 38 1056 1224 1671
Size: 38 1018 168 447
Fragments arranged by size:
1018 447 168 38
Bsp1286I G_dGCh'C
Cuts at: 0 38 1056 1224 1671
Size: 38 1018 168 447
Fragments arranged by size:
1018 447 168 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 64 1671
Size: 64 1607
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1342 1671
Size: 1342 329
BstYI r'GATC_y
Cuts at: 0 1018 1671
Size: 1018 653
Bsu36I CC'TnA_GG
Cuts at: 0 139 1671
Size: 139 1532
BtgI C'CryG_G
Cuts at: 0 44 52 1229 1671
Size: 44 8 1177 442
Fragments arranged by size:
1177 442 44 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 170 1671
Size: 170 1501
BtsI GCAGTG_nn'
Cuts at: 0 1260 1671
Size: 1260 411
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 760 795 1671
Size: 760 35 876
Fragments arranged by size:
876 760 35
DraI TTT'AAA
Cuts at: 0 797 1671
Size: 797 874
EarI CTCTTCn'nnn_
Cuts at: 0 522 953 1671
Size: 522 431 718
Fragments arranged by size:
718 522 431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 431 503 977 986 1671
Size: 374 57 72 474 9 685
Fragments arranged by size:
685 474 374 72 57 9
HaeII r_GCGC'y
Cuts at: 0 52 1671
Size: 52 1619
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 372 404 1671
Size: 372 32 1267
Fragments arranged by size:
1267 372 32
HincII GTy'rAC
Cuts at: 0 114 351 1671
Size: 114 237 1320
Fragments arranged by size:
1320 237 114
HindIII A'AGCT_T
Cuts at: 0 483 986 1671
Size: 483 503 685
Fragments arranged by size:
685 503 483
KasI G'GCGC_C
Cuts at: 0 48 1671
Size: 48 1623
MfeI C'AATT_G
Cuts at: 0 1529 1671
Size: 1529 142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1302 1671
Size: 1302 369
MslI CAynn'nnrTG
Cuts at: 0 1078 1671
Size: 1078 593
NarI GG'CG_CC
Cuts at: 0 49 1671
Size: 49 1622
NcoI C'CATG_G
Cuts at: 0 52 1229 1671
Size: 52 1177 442
Fragments arranged by size:
1177 442 52
NdeI CA'TA_TG
Cuts at: 0 753 1671
Size: 753 918
NsiI A_TGCA'T
Cuts at: 0 1390 1671
Size: 1390 281
PfoI T'CCnGG_A
Cuts at: 0 441 807 1085 1671
Size: 441 366 278 586
Fragments arranged by size:
586 441 366 278
PsiI TTA'TAA
Cuts at: 0 569 1671
Size: 569 1102
SalI G'TCGA_C
Cuts at: 0 112 1671
Size: 112 1559
SfcI C'TryA_G
Cuts at: 0 642 1535 1654 1671
Size: 642 893 119 17
Fragments arranged by size:
893 642 119 17
SfoI GGC'GCC
Cuts at: 0 50 1671
Size: 50 1621
SmlI C'TyrA_G
Cuts at: 0 274 1380 1671
Size: 274 1106 291
Fragments arranged by size:
1106 291 274
SpeI A'CTAG_T
Cuts at: 0 247 1671
Size: 247 1424
SspI AAT'ATT
Cuts at: 0 243 546 1671
Size: 243 303 1125
Fragments arranged by size:
1125 303 243
StyI C'CwwG_G
Cuts at: 0 52 1229 1671
Size: 52 1177 442
Fragments arranged by size:
1177 442 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 221 1671
Size: 45 176 1450
Fragments arranged by size:
1450 176 45
TatI w'GTAC_w
Cuts at: 0 396 651 1671
Size: 396 255 1020
Fragments arranged by size:
1020 396 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 621 653 1671
Size: 621 32 1018
Fragments arranged by size:
1018 621 32
XmnI GAAnn'nnTTC
Cuts at: 0 1375 1611 1671
Size: 1375 236 60
Fragments arranged by size:
1375 236 60
Enzymes that do cut:
AarI AccI AclI AcuI AleI ApoI BanI BbeI
BbsI BcgI BfrBI BmrI BplI BpmI BpuEI BsaBI
BsaHI BseRI BsiHKAI Bsp1286I BspMI BstAPI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI EarI Eco57MI HaeII
Hin4I HincII HindIII KasI MfeI MmeI MslI NarI
NcoI NdeI NsiI PfoI PsiI SalI SfcI SfoI
SmlI SpeI SspI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmtI Bpu10I BsaI BsaAI
BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I ClaI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MluI MscI MspA1I
NaeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SphI SrfI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI