Restriction map for
pF1KB9980
(Linear) (Six-base) MAPSORT of: KIBB9980.seq Check: 1538 from: 1 to: 2124
KIBB9980.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 2124
Size: 1247 877
AflIII A'CryG_T
Cuts at: 0 846 2124
Size: 846 1278
AlwNI CAG_nnn'CTG
Cuts at: 0 1789 2124
Size: 1789 335
ApoI r'AATT_y
Cuts at: 0 125 275 893 1150 1378 1746 2124
Size: 125 150 618 257 228 368 378
Fragments arranged by size:
618 378 368 257 228 150 125
AvaI C'yCGr_G
Cuts at: 0 226 2124
Size: 226 1898
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 95 128 2124
Size: 95 33 1996
Fragments arranged by size:
1996 95 33
BamHI G'GATC_C
Cuts at: 0 544 2124
Size: 544 1580
BbsI GAAGACnn'nnnn_
Cuts at: 0 1487 1964 2124
Size: 1487 477 160
Fragments arranged by size:
1487 477 160
BclI T'GATC_A
Cuts at: 0 285 2124
Size: 285 1839
BfrBI ATG'CAT
Cuts at: 0 888 2124
Size: 888 1236
BglII A'GATC_T
Cuts at: 0 1290 1588 2124
Size: 1290 298 536
Fragments arranged by size:
1290 536 298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1816 2124
Size: 1816 308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 2124
Size: 186 1938
BsaI GGTCTCn'nnnn_
Cuts at: 0 597 1772 2124
Size: 597 1175 352
Fragments arranged by size:
1175 597 352
BsaBI GATnn'nnATC
Cuts at: 0 62 2081 2124
Size: 62 2019 43
Fragments arranged by size:
2019 62 43
BsaWI w'CCGG_w
Cuts at: 0 495 2124
Size: 495 1629
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 821 851 2124
Size: 821 30 1273
Fragments arranged by size:
1273 821 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1408 1510 1615 1775 2124
Size: 1408 102 105 160 349
Fragments arranged by size:
1408 349 160 105 102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 2124
Size: 1351 773
BsiHKAI G_wGCw'C
Cuts at: 0 1584 2124
Size: 1584 540
Bsp1286I G_dGCh'C
Cuts at: 0 1584 2124
Size: 1584 540
BspHI T'CATG_A
Cuts at: 0 186 384 2124
Size: 186 198 1740
Fragments arranged by size:
1740 198 186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 229 2124
Size: 229 1895
BstAPI GCAn_nnn'nTGC
Cuts at: 0 415 2124
Size: 415 1709
BstYI r'GATC_y
Cuts at: 0 544 1290 1588 2124
Size: 544 746 298 536
Fragments arranged by size:
746 544 536 298
BtgI C'CryG_G
Cuts at: 0 319 2124
Size: 319 1805
BtsI GCAGTG_nn'
Cuts at: 0 1339 2124
Size: 1339 785
ClaI AT'CG_AT
Cuts at: 0 1490 2124
Size: 1490 634
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 455 490 2124
Size: 455 35 1634
Fragments arranged by size:
1634 455 35
DraI TTT'AAA
Cuts at: 0 39 1872 2124
Size: 39 1833 252
Fragments arranged by size:
1833 252 39
DraIII CAC_nnn'GTG
Cuts at: 0 415 909 2124
Size: 415 494 1215
Fragments arranged by size:
1215 494 415
DrdI GACnn_nn'nnGTC
Cuts at: 0 1833 2124
Size: 1833 291
EarI CTCTTCn'nnn_
Cuts at: 0 905 1223 1701 1713 1722 1731 1773 2124
Size: 905 318 478 12 9 9 42 351
Fragments arranged by size:
905 478 351 318 42 12 9 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 1816 2124
Size: 1247 569 308
Fragments arranged by size:
1247 569 308
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1265 2124
Size: 1265 859
EcoRI G'AATT_C
Cuts at: 0 1746 2124
Size: 1746 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1045 2124
Size: 1045 1079
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 1217 1249 1669 1701 2044 2076
Size: 607 32 578 32 420 32 343 32
Cuts at: 2076 2124
Size: 48
Fragments arranged by size:
607 578 420 343 48 32 32 32
32
HincII GTy'rAC
Cuts at: 0 297 2124
Size: 297 1827
HindIII A'AGCT_T
Cuts at: 0 999 2124
Size: 999 1125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1412 2124
Size: 781 631 712
Fragments arranged by size:
781 712 631
MslI CAynn'nnrTG
Cuts at: 0 737 1469 2124
Size: 737 732 655
Fragments arranged by size:
737 732 655
MspA1I CmG'CkG
Cuts at: 0 777 2124
Size: 777 1347
NcoI C'CATG_G
Cuts at: 0 319 2124
Size: 319 1805
NsiI A_TGCA'T
Cuts at: 0 890 2124
Size: 890 1234
NspI r_CATG'y
Cuts at: 0 850 2124
Size: 850 1274
PciI A'CATG_T
Cuts at: 0 846 2124
Size: 846 1278
PshAI GACnn'nnGTC
Cuts at: 0 491 1790 2124
Size: 491 1299 334
Fragments arranged by size:
1299 491 334
PspXI vC'TCGA_Gb
Cuts at: 0 226 2124
Size: 226 1898
PstI C_TGCA'G
Cuts at: 0 12 2124
Size: 12 2112
PvuII CAG'CTG
Cuts at: 0 777 2124
Size: 777 1347
SapI GCTCTTCn'nnn_
Cuts at: 0 1773 2124
Size: 1773 351
ScaI AGT'ACT
Cuts at: 0 6 675 1306 2124
Size: 6 669 631 818
Fragments arranged by size:
818 669 631 6
SfcI C'TryA_G
Cuts at: 0 8 1104 2124
Size: 8 1096 1020
Fragments arranged by size:
1096 1020 8
SmlI C'TyrA_G
Cuts at: 0 201 226 2124
Size: 201 25 1898
Fragments arranged by size:
1898 201 25
SpeI A'CTAG_T
Cuts at: 0 2098 2124
Size: 2098 26
SspI AAT'ATT
Cuts at: 0 44 244 2124
Size: 44 200 1880
Fragments arranged by size:
1880 200 44
StyI C'CwwG_G
Cuts at: 0 319 419 2124
Size: 319 100 1705
Fragments arranged by size:
1705 319 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 312 1351 2124
Size: 241 71 1039 773
Fragments arranged by size:
1039 773 241 71
TatI w'GTAC_w
Cuts at: 0 4 673 1304 1371 2124
Size: 4 669 631 67 753
Fragments arranged by size:
753 669 631 67 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 791 823 2124
Size: 791 32 1301
Fragments arranged by size:
1301 791 32
XbaI T'CTAG_A
Cuts at: 0 1843 2124
Size: 1843 281
XhoI C'TCGA_G
Cuts at: 0 226 2124
Size: 226 1898
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AvaI BaeI BamHI BbsI
BclI BfrBI BglII BpmI BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BspMI BstAPI
BstYI BtgI BtsI ClaI CspCI DraI DraIII DrdI
EarI Eco57MI EcoNI EcoRI FalI Hin4I HincII HindIII
MmeI MslI MspA1I NcoI NsiI NspI PciI PshAI
PspXI PstI PvuII SapI ScaI SfcI SmlI SpeI
SspI StyI TaqII TatI TstI XbaI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BanI BanII BbeI BbvCI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BsaAI BsaHI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI EaeI EagI EciI EcoICRI
EcoO109I EcoRV FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI
Tth111I XcmI XmaI XmnI ZraI