Restriction map for
pF1KB9989
(Linear) (Six-base) MAPSORT of: KIBB9989.seq Check: 2564 from: 1 to: 1737
KIBB9989.orf
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 1737
Size: 608 1129
AflII C'TTAA_G
Cuts at: 0 1066 1737
Size: 1066 671
AflIII A'CryG_T
Cuts at: 0 723 1702 1737
Size: 723 979 35
Fragments arranged by size:
979 723 35
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 329 1737
Size: 329 1408
ApoI r'AATT_y
Cuts at: 0 839 1138 1499 1737
Size: 839 299 361 238
Fragments arranged by size:
839 361 299 238
BbsI GAAGACnn'nnnn_
Cuts at: 0 718 1618 1737
Size: 718 900 119
Fragments arranged by size:
900 718 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 146 180 1737
Size: 146 34 1557
Fragments arranged by size:
1557 146 34
BfrBI ATG'CAT
Cuts at: 0 412 556 1737
Size: 412 144 1181
Fragments arranged by size:
1181 412 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 1370 1737
Size: 1370 367
BmtI G_CTAG'C
Cuts at: 0 1429 1737
Size: 1429 308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1737
Size: 224 1513
BsaI GGTCTCn'nnnn_
Cuts at: 0 250 1723 1737
Size: 250 1473 14
Fragments arranged by size:
1473 250 14
BsaBI GATnn'nnATC
Cuts at: 0 434 1737
Size: 434 1303
BsaHI Gr'CG_yC
Cuts at: 0 869 1737
Size: 869 868
BsaWI w'CCGG_w
Cuts at: 0 171 1737
Size: 171 1566
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1214 1244 1392 1422 1737
Size: 1214 30 148 30 315
Fragments arranged by size:
1214 315 148 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 721 1737
Size: 721 1016
BseYI C'CCAG_C
Cuts at: 0 1177 1737
Size: 1177 560
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1067 1252 1685 1737
Size: 896 171 185 433 52
Fragments arranged by size:
896 433 185 171 52
BsiHKAI G_wGCw'C
Cuts at: 0 1306 1470 1737
Size: 1306 164 267
Fragments arranged by size:
1306 267 164
BsmBI CGTCTCn'nnnn_
Cuts at: 0 149 1737
Size: 149 1588
Bsp1286I G_dGCh'C
Cuts at: 0 1306 1470 1737
Size: 1306 164 267
Fragments arranged by size:
1306 267 164
BspEI T'CCGG_A
Cuts at: 0 171 1737
Size: 171 1566
BspHI T'CATG_A
Cuts at: 0 1644 1680 1737
Size: 1644 36 57
Fragments arranged by size:
1644 57 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 743 1737
Size: 743 994
BsrDI GCAATG_nn'
Cuts at: 0 1210 1737
Size: 1210 527
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1389 1528 1737
Size: 1389 139 209
Fragments arranged by size:
1389 209 139
BstBI TT'CG_AA
Cuts at: 0 1215 1737
Size: 1215 522
Bsu36I CC'TnA_GG
Cuts at: 0 773 1737
Size: 773 964
BtgI C'CryG_G
Cuts at: 0 583 923 1737
Size: 583 340 814
Fragments arranged by size:
814 583 340
BtsI GCAGTG_nn'
Cuts at: 0 158 164 1403 1673 1737
Size: 158 6 1239 270 64
Fragments arranged by size:
1239 270 158 64 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 578 613 1737
Size: 578 35 1124
Fragments arranged by size:
1124 578 35
DraI TTT'AAA
Cuts at: 0 543 1638 1737
Size: 543 1095 99
Fragments arranged by size:
1095 543 99
DrdI GACnn_nn'nnGTC
Cuts at: 0 223 1737
Size: 223 1514
EaeI y'GGCC_r
Cuts at: 0 23 900 1737
Size: 23 877 837
Fragments arranged by size:
877 837 23
EarI CTCTTCn'nnn_
Cuts at: 0 626 686 1737
Size: 626 60 1051
Fragments arranged by size:
1051 626 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 608 1737
Size: 224 384 1129
Fragments arranged by size:
1129 384 224
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1056 1737
Size: 1056 681
EcoO109I rG'GnC_Cy
Cuts at: 0 755 1737
Size: 755 982
EcoRV GAT'ATC
Cuts at: 0 1011 1344 1737
Size: 1011 333 393
Fragments arranged by size:
1011 393 333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 407 735 1737
Size: 407 328 1002
Fragments arranged by size:
1002 407 328
HaeII r_GCGC'y
Cuts at: 0 969 1737
Size: 969 768
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 1392 1424 1737
Size: 3 32 1357 32 313
Fragments arranged by size:
1357 313 32 32 3
HincII GTy'rAC
Cuts at: 0 800 1263 1737
Size: 800 463 474
Fragments arranged by size:
800 474 463
MscI TGG'CCA
Cuts at: 0 902 1737
Size: 902 835
MslI CAynn'nnrTG
Cuts at: 0 359 1679 1737
Size: 359 1320 58
Fragments arranged by size:
1320 359 58
MspA1I CmG'CkG
Cuts at: 0 90 1737
Size: 90 1647
NcoI C'CATG_G
Cuts at: 0 583 923 1737
Size: 583 340 814
Fragments arranged by size:
814 583 340
NdeI CA'TA_TG
Cuts at: 0 402 1514 1737
Size: 402 1112 223
Fragments arranged by size:
1112 402 223
NheI G'CTAG_C
Cuts at: 0 1425 1737
Size: 1425 312
NsiI A_TGCA'T
Cuts at: 0 414 558 1737
Size: 414 144 1179
Fragments arranged by size:
1179 414 144
NspI r_CATG'y
Cuts at: 0 727 1433 1706 1737
Size: 727 706 273 31
Fragments arranged by size:
727 706 273 31
PciI A'CATG_T
Cuts at: 0 723 1702 1737
Size: 723 979 35
Fragments arranged by size:
979 723 35
PflMI CCAn_nnn'nTGG
Cuts at: 0 1440 1737
Size: 1440 297
PpuMI rG'GwC_Cy
Cuts at: 0 755 1737
Size: 755 982
PstI C_TGCA'G
Cuts at: 0 1029 1398 1737
Size: 1029 369 339
Fragments arranged by size:
1029 369 339
SapI GCTCTTCn'nnn_
Cuts at: 0 626 1737
Size: 626 1111
SfcI C'TryA_G
Cuts at: 0 464 1025 1394 1626 1737
Size: 464 561 369 232 111
Fragments arranged by size:
561 464 369 232 111
SmlI C'TyrA_G
Cuts at: 0 1066 1737
Size: 1066 671
SphI G_CATG'C
Cuts at: 0 1433 1737
Size: 1433 304
SspI AAT'ATT
Cuts at: 0 564 1737
Size: 564 1173
StyI C'CwwG_G
Cuts at: 0 583 923 1737
Size: 583 340 814
Fragments arranged by size:
814 583 340
TatI w'GTAC_w
Cuts at: 0 1361 1737
Size: 1361 376
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1214 1246 1737
Size: 1214 32 491
Fragments arranged by size:
1214 491 32
Tth111I GACn'n_nGTC
Cuts at: 0 1404 1737
Size: 1404 333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1275 1418 1737
Size: 1275 143 319
Fragments arranged by size:
1275 319 143
Enzymes that do cut:
AcuI AflII AflIII AlfI ApoI BbsI BcgI BfrBI
BmrI BmtI BpmI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BstAPI BstBI Bsu36I BtgI BtsI CspCI
DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII MscI MslI MspA1I NcoI
NdeI NheI NsiI NspI PciI PflMI PpuMI PstI
SapI SfcI SmlI SphI SspI StyI TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BplI
Bpu10I BpuEI BsaAI BsiEI BsiWI BsmI BsrBI BsrFI
BsrGI BssHII BssSI BstEII BstXI BstYI BstZ17I BtgZI
ClaI DraIII EagI EciI EcoICRI EcoRI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NgoMIV NotI NruI PacI PasI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TaqII XbaI XhoI XmaI
XmnI ZraI