Restriction map for
pF1KE0002
(Linear) (Six-base) MAPSORT of: KIEE0002.seq Check: 5873 from: 1 to: 1737
KIEE0002.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 99 208 1737
Size: 99 109 1529
Fragments arranged by size:
1529 109 99
AccI GT'mk_AC
Cuts at: 0 1410 1737
Size: 1410 327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1737
Size: 761 976
AfeI AGC'GCT
Cuts at: 0 722 1737
Size: 722 1015
AflII C'TTAA_G
Cuts at: 0 724 1737
Size: 724 1013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1174 1737
Size: 1174 563
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 505 537 1737
Size: 505 32 1200
Fragments arranged by size:
1200 505 32
AlwNI CAG_nnn'CTG
Cuts at: 0 114 228 510 690 759 1737
Size: 114 114 282 180 69 978
Fragments arranged by size:
978 282 180 114 114 69
ApaI G_GGCC'C
Cuts at: 0 123 928 1218 1387 1737
Size: 123 805 290 169 350
Fragments arranged by size:
805 350 290 169 123
ApoI r'AATT_y
Cuts at: 0 316 1737
Size: 316 1421
AvaI C'yCGr_G
Cuts at: 0 64 122 671 1085 1360 1380 1737
Size: 64 58 549 414 275 20 357
Fragments arranged by size:
549 414 357 275 64 58 20
BanI G'GyrC_C
Cuts at: 0 729 1053 1150 1278 1737
Size: 729 324 97 128 459
Fragments arranged by size:
729 459 324 128 97
BanII G_rGCy'C
Cuts at: 0 123 928 1077 1181 1218 1336 1367 1387
Size: 123 805 149 104 37 118 31 20
Cuts at: 1387 1737
Size: 350
Fragments arranged by size:
805 350 149 123 118 104 37 31
20
BbeI G_GCGC'C
Cuts at: 0 1282 1737
Size: 1282 455
BclI T'GATC_A
Cuts at: 0 230 1737
Size: 230 1507
BglI GCCn_nnn'nGGC
Cuts at: 0 1572 1737
Size: 1572 165
BglII A'GATC_T
Cuts at: 0 968 1737
Size: 968 769
Bme1580I G_kGCm'C
Cuts at: 0 123 504 928 1218 1387 1632 1737
Size: 123 381 424 290 169 245 105
Fragments arranged by size:
424 381 290 245 169 123 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 1465 1737
Size: 1465 272
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 1737
Size: 360 1377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 532 712 886 897 904 1291 1526
Size: 355 177 180 174 11 7 387 235
Cuts at: 1526 1737
Size: 211
Fragments arranged by size:
387 355 235 211 180 177 174 11
7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 1737
Size: 1351 386
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 901 1737
Size: 94 807 836
Fragments arranged by size:
836 807 94
BsaHI Gr'CG_yC
Cuts at: 0 1262 1279 1376 1407 1737
Size: 1262 17 97 31 330
Fragments arranged by size:
1262 330 97 31 17
BsaWI w'CCGG_w
Cuts at: 0 1291 1737
Size: 1291 446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 262 292 505 535 1403 1433 1641 1671
Size: 262 30 213 30 868 30 208 30
Cuts at: 1671 1737
Size: 66
Fragments arranged by size:
868 262 213 208 66 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1138 1141 1559 1631 1737
Size: 1138 3 418 72 106
Fragments arranged by size:
1138 418 106 72 3
BseYI C'CCAG_C
Cuts at: 0 114 353 368 404 1540 1557 1737
Size: 114 239 15 36 1136 17 180
Fragments arranged by size:
1136 239 180 114 36 17 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 517 703 1144 1737
Size: 116 401 186 441 593
Fragments arranged by size:
593 441 401 186 116
BsiEI CG_ry'CG
Cuts at: 0 393 950 1145 1343 1451 1737
Size: 393 557 195 198 108 286
Fragments arranged by size:
557 393 286 198 195 108
BsiHKAI G_wGCw'C
Cuts at: 0 173 1181 1367 1737
Size: 173 1008 186 370
Fragments arranged by size:
1008 370 186 173
BsmBI CGTCTCn'nnnn_
Cuts at: 0 772 1737
Size: 772 965
Bsp1286I G_dGCh'C
Cuts at: 0 123 173 504 928 1077 1181 1218 1336
Size: 123 50 331 424 149 104 37 118
Cuts at: 1336 1367 1387 1632 1737
Size: 31 20 245 105
Fragments arranged by size:
424 331 245 149 123 118 105 104
50 37 31 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 99 208 572 998 1166 1338 1737
Size: 99 109 364 426 168 172 399
Fragments arranged by size:
426 399 364 172 168 109 99
BsrBI CCG'CTC
Cuts at: 0 1131 1737
Size: 1131 606
BsrFI r'CCGG_y
Cuts at: 0 732 1100 1163 1307 1737
Size: 732 368 63 144 430
Fragments arranged by size:
732 430 368 144 63
BstEII G'GTnAC_C
Cuts at: 0 81 1116 1737
Size: 81 1035 621
Fragments arranged by size:
1035 621 81
BstYI r'GATC_y
Cuts at: 0 968 1737
Size: 968 769
BtgI C'CryG_G
Cuts at: 0 1430 1737
Size: 1430 307
BtsI GCAGTG_nn'
Cuts at: 0 1632 1737
Size: 1632 105
DrdI GACnn_nn'nnGTC
Cuts at: 0 951 1737
Size: 951 786
EaeI y'GGCC_r
Cuts at: 0 390 947 1142 1340 1427 1433 1448 1737
Size: 390 557 195 198 87 6 15 289
Fragments arranged by size:
557 390 289 198 195 87 15 6
EagI C'GGCC_G
Cuts at: 0 390 947 1142 1340 1448 1737
Size: 390 557 195 198 108 289
Fragments arranged by size:
557 390 289 198 195 108
EarI CTCTTCn'nnn_
Cuts at: 0 379 1085 1737
Size: 379 706 652
Fragments arranged by size:
706 652 379
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 505 628 1005 1737
Size: 505 123 377 732
Fragments arranged by size:
732 505 377 123
EcoICRI GAG'CTC
Cuts at: 0 1179 1365 1737
Size: 1179 186 372
Fragments arranged by size:
1179 372 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 532 712 761 886 897 904 1291
Size: 355 177 180 49 125 11 7 387
Cuts at: 1291 1526 1737
Size: 235 211
Fragments arranged by size:
387 355 235 211 180 177 125 49
11 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 265 1737
Size: 265 1472
EcoO109I rG'GnC_Cy
Cuts at: 0 119 687 795 924 925 1384 1737
Size: 119 568 108 129 1 459 353
Fragments arranged by size:
568 459 353 129 119 108 1
HaeII r_GCGC'y
Cuts at: 0 724 1282 1737
Size: 724 558 455
Fragments arranged by size:
724 558 455
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 262 294 870 902 1403 1435 1737
Size: 262 32 576 32 501 32 302
Fragments arranged by size:
576 501 302 262 32 32 32
HincII GTy'rAC
Cuts at: 0 1353 1411 1737
Size: 1353 58 326
Fragments arranged by size:
1353 326 58
KasI G'GCGC_C
Cuts at: 0 1278 1737
Size: 1278 459
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1737
Size: 455 1282
MslI CAynn'nnrTG
Cuts at: 0 961 1737
Size: 961 776
MspA1I CmG'CkG
Cuts at: 0 275 528 1258 1499 1737
Size: 275 253 730 241 238
Fragments arranged by size:
730 275 253 241 238
NaeI GCC'GGC
Cuts at: 0 1102 1737
Size: 1102 635
NarI GG'CG_CC
Cuts at: 0 1279 1737
Size: 1279 458
NgoMIV G'CCGG_C
Cuts at: 0 1100 1737
Size: 1100 637
PfoI T'CCnGG_A
Cuts at: 0 57 509 1737
Size: 57 452 1228
Fragments arranged by size:
1228 452 57
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 1737
Size: 505 32 1200
Fragments arranged by size:
1200 505 32
PpuMI rG'GwC_Cy
Cuts at: 0 795 1737
Size: 795 942
PspOMI G'GGCC_C
Cuts at: 0 119 924 1214 1383 1737
Size: 119 805 290 169 354
Fragments arranged by size:
805 354 290 169 119
PstI C_TGCA'G
Cuts at: 0 611 647 751 995 1737
Size: 611 36 104 244 742
Fragments arranged by size:
742 611 244 104 36
SacI G_AGCT'C
Cuts at: 0 1181 1367 1737
Size: 1181 186 370
Fragments arranged by size:
1181 370 186
SalI G'TCGA_C
Cuts at: 0 1409 1737
Size: 1409 328
SanDI GG'GwC_CC
Cuts at: 0 795 1737
Size: 795 942
SapI GCTCTTCn'nnn_
Cuts at: 0 379 1737
Size: 379 1358
SbfI CC_TGCA'GG
Cuts at: 0 995 1737
Size: 995 742
SfcI C'TryA_G
Cuts at: 0 607 643 747 991 1414 1737
Size: 607 36 104 244 423 323
Fragments arranged by size:
607 423 323 244 104 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1572 1737
Size: 1572 165
SfoI GGC'GCC
Cuts at: 0 1280 1737
Size: 1280 457
SmaI CCC'GGG
Cuts at: 0 66 124 1087 1737
Size: 66 58 963 650
Fragments arranged by size:
963 650 66 58
SmlI C'TyrA_G
Cuts at: 0 724 1366 1737
Size: 724 642 371
Fragments arranged by size:
724 642 371
StuI AGG'CCT
Cuts at: 0 260 1737
Size: 260 1477
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 551 1135 1699 1737
Size: 551 584 564 38
Fragments arranged by size:
584 564 551 38
TatI w'GTAC_w
Cuts at: 0 194 542 1737
Size: 194 348 1195
Fragments arranged by size:
1195 348 194
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 692 724 1641 1673 1737
Size: 692 32 917 32 64
Fragments arranged by size:
917 692 64 32 32
XmaI C'CCGG_G
Cuts at: 0 64 122 1085 1737
Size: 64 58 963 652
Fragments arranged by size:
963 652 64 58
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AlfI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BclI BglI
BglII Bme1580I BmrI BplI BpmI BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BstEII BstYI BtgI BtsI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI MmeI MslI MspA1I
NaeI NarI NgoMIV PfoI PpiI PpuMI PspOMI PstI
SacI SalI SanDI SapI SbfI SfcI SfiI SfoI
SmaI SmlI StuI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AhdI AleI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BfrBI BlpI BmgBI BmtI Bpu10I BsaAI
BsaBI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII ScaI SexAI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StyI SwaI Tth111I
XbaI XcmI XhoI XmnI ZraI