Restriction map for
pF1KE0004
(Linear) (Six-base) MAPSORT of: KIEE0004.seq Check: 4530 from: 1 to: 1458
KIEE0004.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 182 1458
Size: 182 1276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 473 1222 1293 1390 1458
Size: 265 208 749 71 97 68
Fragments arranged by size:
749 265 208 97 71 68
AflIII A'CryG_T
Cuts at: 0 302 730 1289 1458
Size: 302 428 559 169
Fragments arranged by size:
559 428 302 169
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 442 474 1458
Size: 442 32 984
Fragments arranged by size:
984 442 32
ApaI G_GGCC'C
Cuts at: 0 1270 1458
Size: 1270 188
AvaI C'yCGr_G
Cuts at: 0 181 468 1458
Size: 181 287 990
Fragments arranged by size:
990 287 181
AvrII C'CTAG_G
Cuts at: 0 1077 1458
Size: 1077 381
BamHI G'GATC_C
Cuts at: 0 794 1458
Size: 794 664
BanI G'GyrC_C
Cuts at: 0 928 940 1458
Size: 928 12 518
Fragments arranged by size:
928 518 12
BanII G_rGCy'C
Cuts at: 0 329 1270 1325 1402 1458
Size: 329 941 55 77 56
Fragments arranged by size:
941 329 77 56 55
BclI T'GATC_A
Cuts at: 0 570 860 1458
Size: 570 290 598
Fragments arranged by size:
598 570 290
Bme1580I G_kGCm'C
Cuts at: 0 943 1270 1458
Size: 943 327 188
Fragments arranged by size:
943 327 188
BmrI ACTGGGnnnn_n'
Cuts at: 0 96 332 1458
Size: 96 236 1126
Fragments arranged by size:
1126 236 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 655 850 1309 1458
Size: 274 381 195 459 149
Fragments arranged by size:
459 381 274 195 149
Bpu10I CC'TnA_GC
Cuts at: 0 1396 1458
Size: 1396 62
BsaAI yAC'GTr
Cuts at: 0 305 1458
Size: 305 1153
BsaHI Gr'CG_yC
Cuts at: 0 622 1458
Size: 622 836
BsaWI w'CCGG_w
Cuts at: 0 455 1110 1458
Size: 455 655 348
Fragments arranged by size:
655 455 348
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 200 230 536 566 1458
Size: 200 30 306 30 892
Fragments arranged by size:
892 306 200 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 83 431 1458
Size: 83 348 1027
Fragments arranged by size:
1027 348 83
BseYI C'CCAG_C
Cuts at: 0 1185 1458
Size: 1185 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1458
Size: 550 908
BsiHKAI G_wGCw'C
Cuts at: 0 188 1458
Size: 188 1270
BsmI GAATG_Cn'
Cuts at: 0 853 1458
Size: 853 605
Bsp1286I G_dGCh'C
Cuts at: 0 188 329 943 1270 1325 1402 1458
Size: 188 141 614 327 55 77 56
Fragments arranged by size:
614 327 188 141 77 56 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 1367 1458
Size: 182 1185 91
Fragments arranged by size:
1185 182 91
BsrDI GCAATG_nn'
Cuts at: 0 358 1458
Size: 358 1100
BsrFI r'CCGG_y
Cuts at: 0 1222 1458
Size: 1222 236
BstYI r'GATC_y
Cuts at: 0 794 1436 1458
Size: 794 642 22
Fragments arranged by size:
794 642 22
Bsu36I CC'TnA_GG
Cuts at: 0 1177 1458
Size: 1177 281
BtgI C'CryG_G
Cuts at: 0 509 1458
Size: 509 949
BtsI GCAGTG_nn'
Cuts at: 0 987 1458
Size: 987 471
EaeI y'GGCC_r
Cuts at: 0 425 1458
Size: 425 1033
EarI CTCTTCn'nnn_
Cuts at: 0 747 1458
Size: 747 711
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 274 473 655 850 1222 1293 1309
Size: 265 9 199 182 195 372 71 16
Cuts at: 1309 1390 1458
Size: 81 68
Fragments arranged by size:
372 265 199 195 182 81 71 68
16 9
EcoO109I rG'GnC_Cy
Cuts at: 0 36 143 1182 1267 1332 1458
Size: 36 107 1039 85 65 126
Fragments arranged by size:
1039 126 107 85 65 36
FspI TGC'GCA
Cuts at: 0 887 1458
Size: 887 571
HaeII r_GCGC'y
Cuts at: 0 1217 1458
Size: 1217 241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1421 1453 1458
Size: 1421 32 5
Fragments arranged by size:
1421 32 5
HincII GTy'rAC
Cuts at: 0 761 1287 1458
Size: 761 526 171
Fragments arranged by size:
761 526 171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 891 1458
Size: 891 567
MslI CAynn'nnrTG
Cuts at: 0 191 1458
Size: 191 1267
NaeI GCC'GGC
Cuts at: 0 1224 1458
Size: 1224 234
NcoI C'CATG_G
Cuts at: 0 509 1458
Size: 509 949
NgoMIV G'CCGG_C
Cuts at: 0 1222 1458
Size: 1222 236
NspI r_CATG'y
Cuts at: 0 759 1293 1458
Size: 759 534 165
Fragments arranged by size:
759 534 165
PasI CC'CwG_GG
Cuts at: 0 996 1458
Size: 996 462
PciI A'CATG_T
Cuts at: 0 1289 1458
Size: 1289 169
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 843 1458
Size: 699 144 615
Fragments arranged by size:
699 615 144
PfoI T'CCnGG_A
Cuts at: 0 1148 1439 1458
Size: 1148 291 19
Fragments arranged by size:
1148 291 19
PpuMI rG'GwC_Cy
Cuts at: 0 143 1182 1458
Size: 143 1039 276
Fragments arranged by size:
1039 276 143
PspOMI G'GGCC_C
Cuts at: 0 1266 1458
Size: 1266 192
PstI C_TGCA'G
Cuts at: 0 507 938 1458
Size: 507 431 520
Fragments arranged by size:
520 507 431
SanDI GG'GwC_CC
Cuts at: 0 1182 1458
Size: 1182 276
SbfI CC_TGCA'GG
Cuts at: 0 938 1458
Size: 938 520
SfcI C'TryA_G
Cuts at: 0 503 934 1352 1458
Size: 503 431 418 106
Fragments arranged by size:
503 431 418 106
StuI AGG'CCT
Cuts at: 0 316 1007 1458
Size: 316 691 451
Fragments arranged by size:
691 451 316
StyI C'CwwG_G
Cuts at: 0 509 698 1077 1458
Size: 509 189 379 381
Fragments arranged by size:
509 381 379 189
TatI w'GTAC_w
Cuts at: 0 1292 1458
Size: 1292 166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 200 232 1144 1176 1458
Size: 200 32 912 32 282
Fragments arranged by size:
912 282 200 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 951 1333 1458
Size: 951 382 125
Fragments arranged by size:
951 382 125
Enzymes that do cut:
AarI AcuI AflIII AloI ApaI AvaI AvrII BamHI
BanI BanII BclI Bme1580I BmrI BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BstYI Bsu36I BtgI BtsI
EaeI EarI Eco57MI EcoO109I FspI HaeII Hin4I HincII
MmeI MslI NaeI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PpuMI PspOMI PstI SanDI SbfI SfcI
StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
BaeI BbeI BbsI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI
KpnI MfeI MluI MscI MspA1I NarI NdeI NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI