Restriction map for
pF1KE0005
(Linear) (Six-base) MAPSORT of: KIEE0005.seq Check: 2545 from: 1 to: 2103
KIEE0005.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
Acc65I G'GTAC_C
Cuts at: 0 1838 2103
Size: 1838 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1369 1760 2098 2103
Size: 653 716 391 338 5
Fragments arranged by size:
716 653 391 338 5
AflIII A'CryG_T
Cuts at: 0 1873 2103
Size: 1873 230
AleI CACnn'nnGTG
Cuts at: 0 857 2103
Size: 857 1246
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1301 1333 2103
Size: 1301 32 770
Fragments arranged by size:
1301 770 32
ApoI r'AATT_y
Cuts at: 0 478 544 681 688 1191 1477 1610 1707
Size: 478 66 137 7 503 286 133 97
Cuts at: 1707 1724 2103
Size: 17 379
Fragments arranged by size:
503 478 379 286 137 133 97 66
17 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 317 350 2103
Size: 317 33 1753
Fragments arranged by size:
1753 317 33
BanI G'GyrC_C
Cuts at: 0 1838 2103
Size: 1838 265
BbsI GAAGACnn'nnnn_
Cuts at: 0 1400 1697 2103
Size: 1400 297 406
Fragments arranged by size:
1400 406 297
BciVI GTATCCnnnnn_n'
Cuts at: 0 1555 1792 2103
Size: 1555 237 311
Fragments arranged by size:
1555 311 237
BclI T'GATC_A
Cuts at: 0 54 2103
Size: 54 2049
BglII A'GATC_T
Cuts at: 0 219 2103
Size: 219 1884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 2103
Size: 1046 1057
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 2103
Size: 205 1898
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1643 1673 2103
Size: 1643 30 430
Fragments arranged by size:
1643 430 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1759 2103
Size: 1759 344
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1684 2103
Size: 1684 419
BsmI GAATG_Cn'
Cuts at: 0 886 2103
Size: 886 1217
BspHI T'CATG_A
Cuts at: 0 399 1974 2103
Size: 399 1575 129
Fragments arranged by size:
1575 399 129
BsrDI GCAATG_nn'
Cuts at: 0 1046 1510 2103
Size: 1046 464 593
Fragments arranged by size:
1046 593 464
BstEII G'GTnAC_C
Cuts at: 0 289 2103
Size: 289 1814
BstYI r'GATC_y
Cuts at: 0 219 2103
Size: 219 1884
Bsu36I CC'TnA_GG
Cuts at: 0 1225 2103
Size: 1225 878
BtsI GCAGTG_nn'
Cuts at: 0 807 1840 2103
Size: 807 1033 263
Fragments arranged by size:
1033 807 263
ClaI AT'CG_AT
Cuts at: 0 149 2103
Size: 149 1954
DraI TTT'AAA
Cuts at: 0 657 699 1196 1884 2103
Size: 657 42 497 688 219
Fragments arranged by size:
688 657 497 219 42
DraIII CAC_nnn'GTG
Cuts at: 0 94 2103
Size: 94 2009
DrdI GACnn_nn'nnGTC
Cuts at: 0 1984 2103
Size: 1984 119
EarI CTCTTCn'nnn_
Cuts at: 0 629 1736 2103
Size: 629 1107 367
Fragments arranged by size:
1107 629 367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1046 1369 1760 2098 2103
Size: 653 393 323 391 338 5
Fragments arranged by size:
653 393 391 338 323 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 922 2103
Size: 922 1181
EcoRI G'AATT_C
Cuts at: 0 478 1477 2103
Size: 478 999 626
Fragments arranged by size:
999 626 478
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1477 2103
Size: 1477 626
FspI TGC'GCA
Cuts at: 0 1579 2103
Size: 1579 524
FspAI rTGC'GCAy
Cuts at: 0 1579 2103
Size: 1579 524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1137 1169 1612 1644 1735 1767 2103
Size: 1137 32 443 32 91 32 336
Fragments arranged by size:
1137 443 336 91 32 32 32
HincII GTy'rAC
Cuts at: 0 747 2103
Size: 747 1356
KpnI G_GTAC'C
Cuts at: 0 1842 2103
Size: 1842 261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 1484 2103
Size: 42 1442 619
Fragments arranged by size:
1442 619 42
MslI CAynn'nnrTG
Cuts at: 0 857 2009 2103
Size: 857 1152 94
Fragments arranged by size:
1152 857 94
NspI r_CATG'y
Cuts at: 0 1877 2103
Size: 1877 226
PciI A'CATG_T
Cuts at: 0 1873 2103
Size: 1873 230
PflMI CCAn_nnn'nTGG
Cuts at: 0 1795 1965 2103
Size: 1795 170 138
Fragments arranged by size:
1795 170 138
ScaI AGT'ACT
Cuts at: 0 1793 2103
Size: 1793 310
SexAI A'CCwGG_T
Cuts at: 0 1730 1911 2103
Size: 1730 181 192
Fragments arranged by size:
1730 192 181
SfcI C'TryA_G
Cuts at: 0 563 1130 1867 1920 2103
Size: 563 567 737 53 183
Fragments arranged by size:
737 567 563 183 53
SmlI C'TyrA_G
Cuts at: 0 184 2103
Size: 184 1919
SspI AAT'ATT
Cuts at: 0 661 1080 1122 1806 2103
Size: 661 419 42 684 297
Fragments arranged by size:
684 661 419 297 42
StuI AGG'CCT
Cuts at: 0 870 1007 2103
Size: 870 137 1096
Fragments arranged by size:
1096 870 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1115 1546 2103
Size: 1115 431 557
Fragments arranged by size:
1115 557 431
TatI w'GTAC_w
Cuts at: 0 278 1791 2103
Size: 278 1513 312
Fragments arranged by size:
1513 312 278
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 752 784 2103
Size: 752 32 1319
Fragments arranged by size:
1319 752 32
Tth111I GACn'n_nGTC
Cuts at: 0 1151 2103
Size: 1151 952
Enzymes that do cut:
Acc65I AcuI AflIII AleI AloI ApoI BaeI BanI
BbsI BciVI BclI BglII BpmI BpuEI BsaXI BseRI
BsgI BsmI BspHI BsrDI BstEII BstYI Bsu36I BtsI
ClaI DraI DraIII DrdI EarI Eco57MI EcoNI EcoRI
FalI FspI FspAI Hin4I HincII KpnI MmeI MslI
NspI PciI PflMI ScaI SexAI SfcI SmlI SspI
StuI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BanII BbeI BbvCI BcgI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgI BtgZI CspCI
EaeI EagI EciI EcoICRI EcoO109I EcoRV FseI HaeII
HindIII HpaI KasI MfeI MluI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StyI
SwaI XbaI XcmI XhoI XmaI XmnI ZraI