Restriction map for
pF1KE0007
(Linear) (Six-base) MAPSORT of: KIEE0007.seq Check: 7801 from: 1 to: 1554
KIEE0007.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 814 1554
Size: 814 740
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 339 1414 1554
Size: 339 1075 140
Fragments arranged by size:
1075 339 140
ApoI r'AATT_y
Cuts at: 0 145 474 888 1554
Size: 145 329 414 666
Fragments arranged by size:
666 414 329 145
BanI G'GyrC_C
Cuts at: 0 1474 1554
Size: 1474 80
BanII G_rGCy'C
Cuts at: 0 488 1554
Size: 488 1066
BbsI GAAGACnn'nnnn_
Cuts at: 0 672 1554
Size: 672 882
BciVI GTATCCnnnnn_n'
Cuts at: 0 36 1554
Size: 36 1518
BmrI ACTGGGnnnn_n'
Cuts at: 0 790 1554
Size: 790 764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 1554
Size: 472 1082
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 771 1441 1554
Size: 631 140 670 113
Fragments arranged by size:
670 631 140 113
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 496 1554
Size: 496 1058
BseYI C'CCAG_C
Cuts at: 0 1225 1448 1554
Size: 1225 223 106
Fragments arranged by size:
1225 223 106
BsiHKAI G_wGCw'C
Cuts at: 0 122 488 496 1554
Size: 122 366 8 1058
Fragments arranged by size:
1058 366 122 8
BsmI GAATG_Cn'
Cuts at: 0 514 1007 1393 1426 1554
Size: 514 493 386 33 128
Fragments arranged by size:
514 493 386 128 33
BsmBI CGTCTCn'nnnn_
Cuts at: 0 526 1399 1554
Size: 526 873 155
Fragments arranged by size:
873 526 155
Bsp1286I G_dGCh'C
Cuts at: 0 122 488 496 1554
Size: 122 366 8 1058
Fragments arranged by size:
1058 366 122 8
BspHI T'CATG_A
Cuts at: 0 1043 1554
Size: 1043 511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 265 1361 1554
Size: 265 1096 193
Fragments arranged by size:
1096 265 193
BsrBI CCG'CTC
Cuts at: 0 1330 1554
Size: 1330 224
BsrFI r'CCGG_y
Cuts at: 0 1471 1554
Size: 1471 83
BsrGI T'GTAC_A
Cuts at: 0 588 672 1245 1554
Size: 588 84 573 309
Fragments arranged by size:
588 573 309 84
BssSI C'ACGA_G
Cuts at: 0 198 1554
Size: 198 1356
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1002 1554
Size: 1002 552
BstXI CCAn_nnnn'nTGG
Cuts at: 0 813 1554
Size: 813 741
BtgI C'CryG_G
Cuts at: 0 96 939 1081 1554
Size: 96 843 142 473
Fragments arranged by size:
843 473 142 96
BtsI GCAGTG_nn'
Cuts at: 0 373 1393 1554
Size: 373 1020 161
Fragments arranged by size:
1020 373 161
DraI TTT'AAA
Cuts at: 0 533 1554
Size: 533 1021
EaeI y'GGCC_r
Cuts at: 0 125 654 1463 1554
Size: 125 529 809 91
Fragments arranged by size:
809 529 125 91
EcoICRI GAG'CTC
Cuts at: 0 486 1554
Size: 486 1068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 1554
Size: 472 1082
EcoO109I rG'GnC_Cy
Cuts at: 0 985 1108 1554
Size: 985 123 446
Fragments arranged by size:
985 446 123
EcoRV GAT'ATC
Cuts at: 0 783 1554
Size: 783 771
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 800 832 1554
Size: 800 32 722
Fragments arranged by size:
800 722 32
HindIII A'AGCT_T
Cuts at: 0 139 1554
Size: 139 1415
MscI TGG'CCA
Cuts at: 0 127 656 1465 1554
Size: 127 529 809 89
Fragments arranged by size:
809 529 127 89
MslI CAynn'nnrTG
Cuts at: 0 466 1418 1554
Size: 466 952 136
Fragments arranged by size:
952 466 136
MspA1I CmG'CkG
Cuts at: 0 1229 1434 1554
Size: 1229 205 120
Fragments arranged by size:
1229 205 120
NcoI C'CATG_G
Cuts at: 0 96 939 1081 1554
Size: 96 843 142 473
Fragments arranged by size:
843 473 142 96
NspI r_CATG'y
Cuts at: 0 1377 1554
Size: 1377 177
PfoI T'CCnGG_A
Cuts at: 0 1346 1554
Size: 1346 208
PpuMI rG'GwC_Cy
Cuts at: 0 985 1108 1554
Size: 985 123 446
Fragments arranged by size:
985 446 123
PshAI GACnn'nnGTC
Cuts at: 0 48 350 1554
Size: 48 302 1204
Fragments arranged by size:
1204 302 48
PsiI TTA'TAA
Cuts at: 0 209 1554
Size: 209 1345
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 959 991 1554
Size: 959 32 563
Fragments arranged by size:
959 563 32
PstI C_TGCA'G
Cuts at: 0 1175 1238 1554
Size: 1175 63 316
Fragments arranged by size:
1175 316 63
PvuII CAG'CTG
Cuts at: 0 1434 1554
Size: 1434 120
SacI G_AGCT'C
Cuts at: 0 488 1554
Size: 488 1066
SfcI C'TryA_G
Cuts at: 0 217 824 1171 1234 1554
Size: 217 607 347 63 320
Fragments arranged by size:
607 347 320 217 63
SmlI C'TyrA_G
Cuts at: 0 646 786 1456 1554
Size: 646 140 670 98
Fragments arranged by size:
670 646 140 98
StyI C'CwwG_G
Cuts at: 0 96 503 939 1081 1554
Size: 96 407 436 142 473
Fragments arranged by size:
473 436 407 142 96
TatI w'GTAC_w
Cuts at: 0 588 672 1245 1554
Size: 588 84 573 309
Fragments arranged by size:
588 573 309 84
Enzymes that do cut:
AhdI AlfI ApoI BanI BanII BbsI BciVI BmrI
BpmI BpuEI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI
BtgI BtsI DraI EaeI EcoICRI Eco57MI EcoO109I EcoRV
Hin4I HindIII MscI MslI MspA1I NcoI NspI PfoI
PpuMI PshAI PsiI PsrI PstI PvuII SacI SfcI
SmlI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsgI BsiEI BsiWI BspEI BsrDI BssHII BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EagI EarI EciI EcoNI EcoRI FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PspOMI PspXI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI