(Linear) (Six-base) MAPSORT of: KIEE0012.seq  Check: 1271  from: 1  to: 1563



KIEE0012.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

Acc65I G'GTAC_C

Cuts at:      0      75    1563 
   Size:         75    1488
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     796    1084    1563 
   Size:        194     602     288     479

  Fragments arranged by size: 

                 602     479     288     194
 
AflII C'TTAA_G

Cuts at:      0     184    1563 
   Size:        184    1379
 
AflIII A'CryG_T

Cuts at:      0     927    1563 
   Size:        927     636
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      30    1563 
   Size:         30    1533
 
ApoI r'AATT_y

Cuts at:      0    1357    1429    1443    1563 
   Size:       1357      72      14     120

  Fragments arranged by size: 

                1357     120      72      14
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     968    1001    1563 
   Size:        968      33     562

  Fragments arranged by size: 

                 968     562      33
 
BanI G'GyrC_C

Cuts at:      0      75    1563 
   Size:         75    1488
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1090    1563 
   Size:       1090     473
 
BclI T'GATC_A

Cuts at:      0     324     591    1563 
   Size:        324     267     972

  Fragments arranged by size: 

                 972     324     267
 
BfrBI ATG'CAT

Cuts at:      0     221    1563 
   Size:        221    1342
 
BglI GCCn_nnn'nGGC

Cuts at:      0     377     921    1563 
   Size:        377     544     642

  Fragments arranged by size: 

                 642     544     377
 
BglII A'GATC_T

Cuts at:      0    1173    1563 
   Size:       1173     390
 
BlpI GC'TnA_GC

Cuts at:      0     661    1563 
   Size:        661     902
 
Bme1580I G_kGCm'C

Cuts at:      0     917    1563 
   Size:        917     646
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      12    1244    1563 
   Size:         12    1232     319

  Fragments arranged by size: 

                1232     319      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     922    1563 
   Size:        922     641
 
BsaBI GATnn'nnATC

Cuts at:      0    1057    1563 
   Size:       1057     506
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1100    1130    1563 
   Size:       1100      30     433

  Fragments arranged by size: 

                1100     433      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     436    1563 
   Size:        436    1127
 
BsmI GAATG_Cn'

Cuts at:      0     373    1563 
   Size:        373    1190
 
Bsp1286I G_dGCh'C

Cuts at:      0     917    1563 
   Size:        917     646
 
BspHI T'CATG_A

Cuts at:      0     861    1563 
   Size:        861     702
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     146     405    1563 
   Size:        146     259    1158

  Fragments arranged by size: 

                1158     259     146
 
BsrDI GCAATG_nn'

Cuts at:      0     539    1563 
   Size:        539    1024
 
BstYI r'GATC_y

Cuts at:      0     135    1173    1544    1563 
   Size:        135    1038     371      19

  Fragments arranged by size: 

                1038     371     135      19
 
Bsu36I CC'TnA_GG

Cuts at:      0    1133    1563 
   Size:       1133     430
 
DraI TTT'AAA

Cuts at:      0      83    1146    1335    1563 
   Size:         83    1063     189     228

  Fragments arranged by size: 

                1063     228     189      83
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     532    1563 
   Size:        532    1031
 
EaeI y'GGCC_r

Cuts at:      0     386     765    1563 
   Size:        386     379     798

  Fragments arranged by size: 

                 798     386     379
 
EarI CTCTTCn'nnn_

Cuts at:      0    1081    1424    1563 
   Size:       1081     343     139

  Fragments arranged by size: 

                1081     343     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     796    1084    1563 
   Size:        194     602     288     479

  Fragments arranged by size: 

                 602     479     288     194
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     950    1563 
   Size:        950     613
 
EcoO109I rG'GnC_Cy

Cuts at:      0     490    1563 
   Size:        490    1073
 
EcoRV GAT'ATC

Cuts at:      0     567    1563 
   Size:        567     996
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     199     453    1563 
   Size:        199     254    1110

  Fragments arranged by size: 

                1110     254     199
 
KpnI G_GTAC'C

Cuts at:      0      79    1563 
   Size:         79    1484
 
MfeI C'AATT_G

Cuts at:      0    1315    1563 
   Size:       1315     248
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     193    1563 
   Size:        193    1370
 
MscI TGG'CCA

Cuts at:      0     388     767    1563 
   Size:        388     379     796

  Fragments arranged by size: 

                 796     388     379
 
MslI CAynn'nnrTG

Cuts at:      0     269     599    1210    1563 
   Size:        269     330     611     353

  Fragments arranged by size: 

                 611     353     330     269
 
MspA1I CmG'CkG

Cuts at:      0    1192    1563 
   Size:       1192     371
 
NsiI A_TGCA'T

Cuts at:      0     223    1563 
   Size:        223    1340
 
NspI r_CATG'y

Cuts at:      0     450     931    1563 
   Size:        450     481     632

  Fragments arranged by size: 

                 632     481     450
 
PciI A'CATG_T

Cuts at:      0     927    1563 
   Size:        927     636
 
PpuMI rG'GwC_Cy

Cuts at:      0     490    1563 
   Size:        490    1073
 
PsiI TTA'TAA

Cuts at:      0     239    1563 
   Size:        239    1324
 
PvuII CAG'CTG

Cuts at:      0    1192    1563 
   Size:       1192     371
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1424    1563 
   Size:       1424     139
 
SfcI C'TryA_G

Cuts at:      0     822    1563 
   Size:        822     741
 
SmlI C'TyrA_G

Cuts at:      0     184     937    1563 
   Size:        184     753     626

  Fragments arranged by size: 

                 753     626     184
 
SphI G_CATG'C

Cuts at:      0     450    1563 
   Size:        450    1113
 
StyI C'CwwG_G

Cuts at:      0     799     845    1563 
   Size:        799      46     718

  Fragments arranged by size: 

                 799     718      46
 
TatI w'GTAC_w

Cuts at:      0     818    1563 
   Size:        818     745
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     509    1563 
   Size:        509    1054
 
XmnI GAAnn'nnTTC

Cuts at:      0    1019    1361    1563 
   Size:       1019     342     202

  Fragments arranged by size: 

                1019     342     202
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     AloI     ApoI     BaeI     BanI
     BbsI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
    BpuEI    BsaBI    BsaXI    BseRI     BsmI Bsp1286I    BspHI    BspMI
    BsrDI    BstYI   Bsu36I     DraI     DrdI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NsiI     NspI     PciI    PpuMI     PsiI    PvuII
     SapI     SfcI     SmlI     SphI     StyI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI    BmgBI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     EagI
     EciI  EcoICRI    EcoRI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     MluI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI