Restriction map for
pF1KE0016
(Linear) (Six-base) MAPSORT of: KIEE0016.seq Check: 231 from: 1 to: 2223
KIEE0016.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AatII G_ACGT'C
Cuts at: 0 374 2223
Size: 374 1849
AccI GT'mk_AC
Cuts at: 0 219 2223
Size: 219 2004
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 1145 1931 2033 2223
Size: 795 350 786 102 190
Fragments arranged by size:
795 786 350 190 102
AleI CACnn'nnGTG
Cuts at: 0 898 2223
Size: 898 1325
AlwNI CAG_nnn'CTG
Cuts at: 0 1055 2223
Size: 1055 1168
ApaI G_GGCC'C
Cuts at: 0 883 2223
Size: 883 1340
ApaLI G'TGCA_C
Cuts at: 0 481 2223
Size: 481 1742
ApoI r'AATT_y
Cuts at: 0 721 2223
Size: 721 1502
AseI AT'TA_AT
Cuts at: 0 1939 2223
Size: 1939 284
AvaI C'yCGr_G
Cuts at: 0 294 1768 2223
Size: 294 1474 455
Fragments arranged by size:
1474 455 294
BamHI G'GATC_C
Cuts at: 0 1145 2223
Size: 1145 1078
BanII G_rGCy'C
Cuts at: 0 883 1293 2223
Size: 883 410 930
Fragments arranged by size:
930 883 410
BbsI GAAGACnn'nnnn_
Cuts at: 0 366 1133 2223
Size: 366 767 1090
Fragments arranged by size:
1090 767 366
BbvCI CC'TCA_GC
Cuts at: 0 1604 2223
Size: 1604 619
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 512 2223
Size: 478 34 1711
Fragments arranged by size:
1711 478 34
BclI T'GATC_A
Cuts at: 0 1950 2223
Size: 1950 273
BglI GCCn_nnn'nGGC
Cuts at: 0 439 1097 2223
Size: 439 658 1126
Fragments arranged by size:
1126 658 439
BglII A'GATC_T
Cuts at: 0 1785 2223
Size: 1785 438
Bme1580I G_kGCm'C
Cuts at: 0 485 534 877 883 2223
Size: 485 49 343 6 1340
Fragments arranged by size:
1340 485 343 49 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 882 1077 2223
Size: 854 28 195 1146
Fragments arranged by size:
1146 854 195 28
Bpu10I CC'TnA_GC
Cuts at: 0 790 1294 1445 1604 2223
Size: 790 504 151 159 619
Fragments arranged by size:
790 619 504 159 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2011 2223
Size: 2011 212
BsaAI yAC'GTr
Cuts at: 0 324 855 1800 2223
Size: 324 531 945 423
Fragments arranged by size:
945 531 423 324
BsaBI GATnn'nnATC
Cuts at: 0 23 1790 2223
Size: 23 1767 433
Fragments arranged by size:
1767 433 23
BsaHI Gr'CG_yC
Cuts at: 0 371 442 1773 2223
Size: 371 71 1331 450
Fragments arranged by size:
1331 450 371 71
BsaWI w'CCGG_w
Cuts at: 0 1718 2223
Size: 1718 505
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 110 140 898 928 1600 1630 2223
Size: 110 30 758 30 672 30 593
Fragments arranged by size:
758 672 593 110 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 796 1621 1870 2223
Size: 796 825 249 353
Fragments arranged by size:
825 796 353 249
BseYI C'CCAG_C
Cuts at: 0 681 841 1249 1338 1983 2223
Size: 681 160 408 89 645 240
Fragments arranged by size:
681 645 408 240 160 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 409 536 1696 2223
Size: 325 84 127 1160 527
Fragments arranged by size:
1160 527 325 127 84
BsiEI CG_ry'CG
Cuts at: 0 513 2223
Size: 513 1710
BsiHKAI G_wGCw'C
Cuts at: 0 485 1123 1422 1850 2223
Size: 485 638 299 428 373
Fragments arranged by size:
638 485 428 373 299
BsmI GAATG_Cn'
Cuts at: 0 600 978 1818 2223
Size: 600 378 840 405
Fragments arranged by size:
840 600 405 378
Bsp1286I G_dGCh'C
Cuts at: 0 485 534 877 883 1123 1293 1422 1850
Size: 485 49 343 6 240 170 129 428
Cuts at: 1850 2223
Size: 373
Fragments arranged by size:
485 428 373 343 240 170 129 49
6
BspHI T'CATG_A
Cuts at: 0 201 2223
Size: 201 2022
BsrDI GCAATG_nn'
Cuts at: 0 1322 2223
Size: 1322 901
BsrFI r'CCGG_y
Cuts at: 0 251 885 2223
Size: 251 634 1338
Fragments arranged by size:
1338 634 251
BstYI r'GATC_y
Cuts at: 0 1145 1785 2223
Size: 1145 640 438
Fragments arranged by size:
1145 640 438
BtgI C'CryG_G
Cuts at: 0 311 2064 2150 2223
Size: 311 1753 86 73
Fragments arranged by size:
1753 311 86 73
BtsI GCAGTG_nn'
Cuts at: 0 650 2223
Size: 650 1573
DraI TTT'AAA
Cuts at: 0 689 2223
Size: 689 1534
DraIII CAC_nnn'GTG
Cuts at: 0 312 491 2223
Size: 312 179 1732
Fragments arranged by size:
1732 312 179
EaeI y'GGCC_r
Cuts at: 0 2 452 510 647 1131 2111 2223
Size: 2 450 58 137 484 980 112
Fragments arranged by size:
980 484 450 137 112 58 2
EagI C'GGCC_G
Cuts at: 0 510 2223
Size: 510 1713
EarI CTCTTCn'nnn_
Cuts at: 0 814 1917 2223
Size: 814 1103 306
Fragments arranged by size:
1103 814 306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1407 2223
Size: 1407 816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 854 882 1077 1145 1931 2033 2223
Size: 795 59 28 195 68 786 102 190
Fragments arranged by size:
795 786 195 190 102 68 59 28
EcoO109I rG'GnC_Cy
Cuts at: 0 162 189 801 2223
Size: 162 27 612 1422
Fragments arranged by size:
1422 612 162 27
FspI TGC'GCA
Cuts at: 0 1378 2223
Size: 1378 845
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 361 393 1137 1169 1600 1632
Size: 110 32 219 32 744 32 431 32
Cuts at: 1632 2223
Size: 591
Fragments arranged by size:
744 591 431 219 110 32 32 32
32
HincII GTy'rAC
Cuts at: 0 153 330 2223
Size: 153 177 1893
Fragments arranged by size:
1893 177 153
HindIII A'AGCT_T
Cuts at: 0 2015 2223
Size: 2015 208
HpaI GTT'AAC
Cuts at: 0 153 2223
Size: 153 2070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1899 2223
Size: 1899 324
MscI TGG'CCA
Cuts at: 0 649 2113 2223
Size: 649 1464 110
Fragments arranged by size:
1464 649 110
MslI CAynn'nnrTG
Cuts at: 0 709 898 934 1802 2223
Size: 709 189 36 868 421
Fragments arranged by size:
868 709 421 189 36
MspA1I CmG'CkG
Cuts at: 0 1330 1382 1498 1651 2223
Size: 1330 52 116 153 572
Fragments arranged by size:
1330 572 153 116 52
NaeI GCC'GGC
Cuts at: 0 887 2223
Size: 887 1336
NcoI C'CATG_G
Cuts at: 0 2064 2223
Size: 2064 159
NgoMIV G'CCGG_C
Cuts at: 0 885 2223
Size: 885 1338
NspI r_CATG'y
Cuts at: 0 789 933 2223
Size: 789 144 1290
Fragments arranged by size:
1290 789 144
PasI CC'CwG_GG
Cuts at: 0 183 437 2223
Size: 183 254 1786
Fragments arranged by size:
1786 254 183
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 614 2223
Size: 312 302 1609
Fragments arranged by size:
1609 312 302
PmlI CAC'GTG
Cuts at: 0 324 1800 2223
Size: 324 1476 423
Fragments arranged by size:
1476 423 324
PpuMI rG'GwC_Cy
Cuts at: 0 162 189 2223
Size: 162 27 2034
Fragments arranged by size:
2034 162 27
PspOMI G'GGCC_C
Cuts at: 0 879 2223
Size: 879 1344
PstI C_TGCA'G
Cuts at: 0 401 2223
Size: 401 1822
PvuII CAG'CTG
Cuts at: 0 1498 2223
Size: 1498 725
SfcI C'TryA_G
Cuts at: 0 397 1594 2223
Size: 397 1197 629
Fragments arranged by size:
1197 629 397
SmlI C'TyrA_G
Cuts at: 0 2026 2223
Size: 2026 197
SphI G_CATG'C
Cuts at: 0 789 933 2223
Size: 789 144 1290
Fragments arranged by size:
1290 789 144
StyI C'CwwG_G
Cuts at: 0 555 2064 2223
Size: 555 1509 159
Fragments arranged by size:
1509 555 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 606 2223
Size: 606 1617
TatI w'GTAC_w
Cuts at: 0 401 2223
Size: 401 1822
Tth111I GACn'n_nGTC
Cuts at: 0 124 2223
Size: 124 2099
XbaI T'CTAG_A
Cuts at: 0 2103 2223
Size: 2103 120
ZraI GAC'GTC
Cuts at: 0 372 2223
Size: 372 1851
Enzymes that do cut:
AatII AccI AcuI AleI AlwNI ApaI ApaLI ApoI
AseI AvaI BamHI BanII BbsI BbvCI BcgI BclI
BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrFI BstYI BtgI BtsI
DraI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I
FspI Hin4I HincII HindIII HpaI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PflMI PmlI
PpuMI PspOMI PstI PvuII SfcI SmlI SphI StyI
TaqII TatI Tth111I XbaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AscI AsiSI AvrII BaeI BanI BbeI
BciVI BfrBI BlpI BmgBI BmrI BmtI BplI BsaI
BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspAI HaeII KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TstI XcmI XhoI XmaI XmnI