Restriction map for
pF1KE0019
(Linear) (Six-base) MAPSORT of: KIEE0019.seq Check: 3005 from: 1 to: 1776
KIEE0019.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 935 1776
Size: 935 841
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1039 1776
Size: 1039 737
ApaI G_GGCC'C
Cuts at: 0 276 1776
Size: 276 1500
ApaLI G'TGCA_C
Cuts at: 0 1628 1776
Size: 1628 148
ApoI r'AATT_y
Cuts at: 0 925 1776
Size: 925 851
AseI AT'TA_AT
Cuts at: 0 1103 1776
Size: 1103 673
BanII G_rGCy'C
Cuts at: 0 276 1364 1776
Size: 276 1088 412
Fragments arranged by size:
1088 412 276
BbsI GAAGACnn'nnnn_
Cuts at: 0 1262 1776
Size: 1262 514
BbvCI CC'TCA_GC
Cuts at: 0 1008 1776
Size: 1008 768
BclI T'GATC_A
Cuts at: 0 1697 1776
Size: 1697 79
Bme1580I G_kGCm'C
Cuts at: 0 276 1632 1776
Size: 276 1356 144
Fragments arranged by size:
1356 276 144
BmrI ACTGGGnnnn_n'
Cuts at: 0 228 1731 1776
Size: 228 1503 45
Fragments arranged by size:
1503 228 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1071 1776
Size: 1071 705
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1583 1776
Size: 571 1012 193
Fragments arranged by size:
1012 571 193
Bpu10I CC'TnA_GC
Cuts at: 0 1008 1776
Size: 1008 768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1776
Size: 136 1640
BsaI GGTCTCn'nnnn_
Cuts at: 0 387 895 1776
Size: 387 508 881
Fragments arranged by size:
881 508 387
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 932 935 1776
Size: 932 3 841
Fragments arranged by size:
932 841 3
BseYI C'CCAG_C
Cuts at: 0 635 1776
Size: 635 1141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1316 1633 1776
Size: 1057 259 317 143
Fragments arranged by size:
1057 317 259 143
BsiHKAI G_wGCw'C
Cuts at: 0 1052 1632 1776
Size: 1052 580 144
Fragments arranged by size:
1052 580 144
Bsp1286I G_dGCh'C
Cuts at: 0 276 1052 1364 1632 1776
Size: 276 776 312 268 144
Fragments arranged by size:
776 312 276 268 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1010 1655 1776
Size: 1010 645 121
Fragments arranged by size:
1010 645 121
BsrGI T'GTAC_A
Cuts at: 0 336 1776
Size: 336 1440
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1406 1776
Size: 1406 370
BstYI r'GATC_y
Cuts at: 0 693 1776
Size: 693 1083
BtgI C'CryG_G
Cuts at: 0 1668 1776
Size: 1668 108
BtsI GCAGTG_nn'
Cuts at: 0 1304 1776
Size: 1304 472
DraI TTT'AAA
Cuts at: 0 497 1776
Size: 497 1279
DraIII CAC_nnn'GTG
Cuts at: 0 1627 1776
Size: 1627 149
EaeI y'GGCC_r
Cuts at: 0 321 1379 1776
Size: 321 1058 397
Fragments arranged by size:
1058 397 321
EarI CTCTTCn'nnn_
Cuts at: 0 1040 1776
Size: 1040 736
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 935 1583 1776
Size: 571 364 648 193
Fragments arranged by size:
648 571 364 193
EcoO109I rG'GnC_Cy
Cuts at: 0 272 1776
Size: 272 1504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 348 380 991 1023 1776
Size: 348 32 611 32 753
Fragments arranged by size:
753 611 348 32 32
HindIII A'AGCT_T
Cuts at: 0 1509 1776
Size: 1509 267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 225 1399 1547 1776
Size: 46 179 1174 148 229
Fragments arranged by size:
1174 229 179 148 46
MscI TGG'CCA
Cuts at: 0 323 1381 1776
Size: 323 1058 395
Fragments arranged by size:
1058 395 323
MslI CAynn'nnrTG
Cuts at: 0 185 1690 1776
Size: 185 1505 86
Fragments arranged by size:
1505 185 86
MspA1I CmG'CkG
Cuts at: 0 334 1012 1776
Size: 334 678 764
Fragments arranged by size:
764 678 334
NdeI CA'TA_TG
Cuts at: 0 1223 1776
Size: 1223 553
NspI r_CATG'y
Cuts at: 0 1026 1594 1776
Size: 1026 568 182
Fragments arranged by size:
1026 568 182
PspOMI G'GGCC_C
Cuts at: 0 272 1776
Size: 272 1504
PstI C_TGCA'G
Cuts at: 0 1555 1776
Size: 1555 221
PvuII CAG'CTG
Cuts at: 0 334 1012 1776
Size: 334 678 764
Fragments arranged by size:
764 678 334
SapI GCTCTTCn'nnn_
Cuts at: 0 1040 1776
Size: 1040 736
SfcI C'TryA_G
Cuts at: 0 1180 1551 1776
Size: 1180 371 225
Fragments arranged by size:
1180 371 225
SmlI C'TyrA_G
Cuts at: 0 115 1776
Size: 115 1661
SphI G_CATG'C
Cuts at: 0 1026 1776
Size: 1026 750
SspI AAT'ATT
Cuts at: 0 165 1492 1776
Size: 165 1327 284
Fragments arranged by size:
1327 284 165
StuI AGG'CCT
Cuts at: 0 1033 1776
Size: 1033 743
TatI w'GTAC_w
Cuts at: 0 336 1246 1776
Size: 336 910 530
Fragments arranged by size:
910 530 336
XbaI T'CTAG_A
Cuts at: 0 939 1432 1776
Size: 939 493 344
Fragments arranged by size:
939 493 344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 241 643 1089 1776
Size: 241 402 446 687
Fragments arranged by size:
687 446 402 241
XmnI GAAnn'nnTTC
Cuts at: 0 197 1776
Size: 197 1579
Enzymes that do cut:
AcuI AlfI ApaI ApaLI ApoI AseI BanII BbsI
BbvCI BclI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrGI
BstAPI BstYI BtgI BtsI DraI DraIII EaeI EarI
Eco57MI EcoO109I Hin4I HindIII MmeI MscI MslI MspA1I
NdeI NspI PspOMI PstI PvuII SapI SfcI SmlI
SphI SspI StuI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StyI SwaI TaqII
TstI Tth111I XhoI XmaI ZraI