Restriction map for
pF1KE0023
(Linear) (Six-base) MAPSORT of: KIEE0023.seq Check: 1285 from: 1 to: 2865
KIEE0023.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 995 2865
Size: 995 1870
AclI AA'CG_TT
Cuts at: 0 963 2865
Size: 963 1902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1688 1982 2539 2865
Size: 701 987 294 557 326
Fragments arranged by size:
987 701 557 326 294
AfeI AGC'GCT
Cuts at: 0 1355 2865
Size: 1355 1510
AflIII A'CryG_T
Cuts at: 0 131 2004 2865
Size: 131 1873 861
Fragments arranged by size:
1873 861 131
ApoI r'AATT_y
Cuts at: 0 223 262 435 533 689 1024 1144 1501
Size: 223 39 173 98 156 335 120 357
Cuts at: 1501 1515 1897 2262 2562 2865
Size: 14 382 365 300 303
Fragments arranged by size:
382 365 357 335 303 300 223 173
156 120 98 39 14
AseI AT'TA_AT
Cuts at: 0 821 2865
Size: 821 2044
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1916 1949 2865
Size: 1916 33 916
Fragments arranged by size:
1916 916 33
BanII G_rGCy'C
Cuts at: 0 7 1783 2602 2865
Size: 7 1776 819 263
Fragments arranged by size:
1776 819 263 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 2816 2865
Size: 2816 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 257 291 2679 2713 2865
Size: 257 34 2388 34 152
Fragments arranged by size:
2388 257 152 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1286 2865
Size: 1286 1579
BclI T'GATC_A
Cuts at: 0 975 2865
Size: 975 1890
BfrBI ATG'CAT
Cuts at: 0 494 2421 2865
Size: 494 1927 444
Fragments arranged by size:
1927 494 444
BglII A'GATC_T
Cuts at: 0 613 1197 2865
Size: 613 584 1668
Fragments arranged by size:
1668 613 584
BmgBI CAC'GTC
Cuts at: 0 2693 2865
Size: 2693 172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2157 2621 2865
Size: 2157 464 244
Fragments arranged by size:
2157 464 244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1736 2422 2453 2726 2865
Size: 1736 686 31 273 139
Fragments arranged by size:
1736 686 273 139 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 630 2865
Size: 311 319 2235
Fragments arranged by size:
2235 319 311
BsaI GGTCTCn'nnnn_
Cuts at: 0 1012 2720 2865
Size: 1012 1708 145
Fragments arranged by size:
1708 1012 145
BsaAI yAC'GTr
Cuts at: 0 517 2865
Size: 517 2348
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1346 2203 2865
Size: 1346 857 662
Fragments arranged by size:
1346 857 662
BsiHKAI G_wGCw'C
Cuts at: 0 7 1783 2865
Size: 7 1776 1082
Fragments arranged by size:
1776 1082 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 1783 2602 2865
Size: 7 1776 819 263
Fragments arranged by size:
1776 819 263 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 588 2865
Size: 588 2277
BsrDI GCAATG_nn'
Cuts at: 0 86 273 413 494 2865
Size: 86 187 140 81 2371
Fragments arranged by size:
2371 187 140 86 81
BsrGI T'GTAC_A
Cuts at: 0 2616 2865
Size: 2616 249
BstAPI GCAn_nnn'nTGC
Cuts at: 0 88 2865
Size: 88 2777
BstEII G'GTnAC_C
Cuts at: 0 15 2865
Size: 15 2850
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1321 2865
Size: 1321 1544
BstYI r'GATC_y
Cuts at: 0 613 1047 1197 2865
Size: 613 434 150 1668
Fragments arranged by size:
1668 613 434 150
BtsI GCAGTG_nn'
Cuts at: 0 2187 2865
Size: 2187 678
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 464 499 2865
Size: 464 35 2366
Fragments arranged by size:
2366 464 35
DraI TTT'AAA
Cuts at: 0 2304 2397 2801 2865
Size: 2304 93 404 64
Fragments arranged by size:
2304 404 93 64
EaeI y'GGCC_r
Cuts at: 0 1944 2865
Size: 1944 921
EarI CTCTTCn'nnn_
Cuts at: 0 1619 1958 2180 2438 2501 2865
Size: 1619 339 222 258 63 364
Fragments arranged by size:
1619 364 339 258 222 63
EcoICRI GAG'CTC
Cuts at: 0 5 1781 2865
Size: 5 1776 1084
Fragments arranged by size:
1776 1084 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1688 1736 1982 2422 2453 2539 2726
Size: 701 987 48 246 440 31 86 187
Cuts at: 2726 2865
Size: 139
Fragments arranged by size:
987 701 440 246 187 139 86 48
31
EcoRI G'AATT_C
Cuts at: 0 262 2262 2562 2865
Size: 262 2000 300 303
Fragments arranged by size:
2000 303 300 262
EcoRV GAT'ATC
Cuts at: 0 2276 2865
Size: 2276 589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1165 2865
Size: 1165 1700
HaeII r_GCGC'y
Cuts at: 0 61 1357 2865
Size: 61 1296 1508
Fragments arranged by size:
1508 1296 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1222 1254 1443 1475 2865
Size: 1222 32 189 32 1390
Fragments arranged by size:
1390 1222 189 32 32
HincII GTy'rAC
Cuts at: 0 786 2865
Size: 786 2079
HindIII A'AGCT_T
Cuts at: 0 2110 2865
Size: 2110 755
HpaI GTT'AAC
Cuts at: 0 786 2865
Size: 786 2079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 2865
Size: 1162 1703
MscI TGG'CCA
Cuts at: 0 1946 2865
Size: 1946 919
MslI CAynn'nnrTG
Cuts at: 0 278 1450 2024 2865
Size: 278 1172 574 841
Fragments arranged by size:
1172 841 574 278
NdeI CA'TA_TG
Cuts at: 0 1058 2865
Size: 1058 1807
NsiI A_TGCA'T
Cuts at: 0 496 2423 2865
Size: 496 1927 442
Fragments arranged by size:
1927 496 442
NspI r_CATG'y
Cuts at: 0 135 2008 2777 2865
Size: 135 1873 769 88
Fragments arranged by size:
1873 769 135 88
PciI A'CATG_T
Cuts at: 0 131 2004 2865
Size: 131 1873 861
Fragments arranged by size:
1873 861 131
PfoI T'CCnGG_A
Cuts at: 0 2339 2865
Size: 2339 526
PstI C_TGCA'G
Cuts at: 0 56 2865
Size: 56 2809
SacI G_AGCT'C
Cuts at: 0 7 1783 2865
Size: 7 1776 1082
Fragments arranged by size:
1776 1082 7
SapI GCTCTTCn'nnn_
Cuts at: 0 2501 2865
Size: 2501 364
ScaI AGT'ACT
Cuts at: 0 1433 2865
Size: 1433 1432
SfcI C'TryA_G
Cuts at: 0 52 1193 2865
Size: 52 1141 1672
Fragments arranged by size:
1672 1141 52
SmlI C'TyrA_G
Cuts at: 0 326 609 2865
Size: 326 283 2256
Fragments arranged by size:
2256 326 283
SspI AAT'ATT
Cuts at: 0 825 2204 2865
Size: 825 1379 661
Fragments arranged by size:
1379 825 661
StyI C'CwwG_G
Cuts at: 0 829 1346 2865
Size: 829 517 1519
Fragments arranged by size:
1519 829 517
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3 692 759 2164 2865
Size: 3 689 67 1405 701
Fragments arranged by size:
1405 701 689 67 3
TatI w'GTAC_w
Cuts at: 0 1431 2616 2865
Size: 1431 1185 249
Fragments arranged by size:
1431 1185 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 89 121 288 320 2865
Size: 89 32 167 32 2545
Fragments arranged by size:
2545 167 89 32 32
XbaI T'CTAG_A
Cuts at: 0 2436 2865
Size: 2436 429
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2397 2865
Size: 2397 468
XmnI GAAnn'nnTTC
Cuts at: 0 689 1829 2289 2865
Size: 689 1140 460 576
Fragments arranged by size:
1140 689 576 460
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII ApoI AseI BaeI
BanII BbsI BcgI BciVI BclI BfrBI BglII BmgBI
BplI BpmI BpuEI BsaI BsaAI BseRI BsiHKAI Bsp1286I
BspMI BsrDI BsrGI BstAPI BstEII BstXI BstYI BtsI
CspCI DraI EaeI EarI EcoICRI Eco57MI EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII HpaI MmeI MscI
MslI NdeI NsiI NspI PciI PfoI PstI SacI
SapI ScaI SfcI SmlI SspI StyI TaqII TatI
TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BamHI BanI BbeI BbvCI BglI BlpI Bme1580I BmrI
BmtI Bpu10I BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstBI BstZ17I Bsu36I BtgI BtgZI ClaI
DraIII DrdI EagI EciI EcoNI EcoO109I FseI FspI
FspAI KasI KpnI MfeI MluI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XhoI XmaI ZraI