Restriction map for
pF1KE0026
(Linear) (Six-base) MAPSORT of: KIEE0026.seq Check: 1308 from: 1 to: 1455
KIEE0026.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 1136 1154 1455
Size: 1136 18 301
Fragments arranged by size:
1136 301 18
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 334 475 913 1455
Size: 298 36 141 438 542
Fragments arranged by size:
542 438 298 141 36
AflIII A'CryG_T
Cuts at: 0 969 1455
Size: 969 486
AlwNI CAG_nnn'CTG
Cuts at: 0 104 678 720 931 999 1455
Size: 104 574 42 211 68 456
Fragments arranged by size:
574 456 211 104 68 42
ApaLI G'TGCA_C
Cuts at: 0 973 1455
Size: 973 482
BamHI G'GATC_C
Cuts at: 0 3 921 1455
Size: 3 918 534
Fragments arranged by size:
918 534 3
BanI G'GyrC_C
Cuts at: 0 1219 1367 1455
Size: 1219 148 88
Fragments arranged by size:
1219 148 88
BanII G_rGCy'C
Cuts at: 0 77 186 701 1455
Size: 77 109 515 754
Fragments arranged by size:
754 515 109 77
BbeI G_GCGC'C
Cuts at: 0 1371 1455
Size: 1371 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 862 901 1455
Size: 862 39 554
Fragments arranged by size:
862 554 39
BbvCI CC'TCA_GC
Cuts at: 0 1192 1455
Size: 1192 263
BglI GCCn_nnn'nGGC
Cuts at: 0 216 1455
Size: 216 1239
Bme1580I G_kGCm'C
Cuts at: 0 977 1455
Size: 977 478
BmrI ACTGGGnnnn_n'
Cuts at: 0 165 883 1086 1455
Size: 165 718 203 369
Fragments arranged by size:
718 369 203 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 868 1455
Size: 700 168 587
Fragments arranged by size:
700 587 168
Bpu10I CC'TnA_GC
Cuts at: 0 253 1192 1455
Size: 253 939 263
Fragments arranged by size:
939 263 253
BsaI GGTCTCn'nnnn_
Cuts at: 0 658 1455
Size: 658 797
BsaHI Gr'CG_yC
Cuts at: 0 1368 1455
Size: 1368 87
BsaWI w'CCGG_w
Cuts at: 0 1139 1455
Size: 1139 316
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 1312 1342 1455
Size: 409 30 873 30 113
Fragments arranged by size:
873 409 113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1050 1247 1412 1455
Size: 1050 197 165 43
Fragments arranged by size:
1050 197 165 43
BseYI C'CCAG_C
Cuts at: 0 612 1455
Size: 612 843
BsiHKAI G_wGCw'C
Cuts at: 0 186 701 977 1455
Size: 186 515 276 478
Fragments arranged by size:
515 478 276 186
BsmBI CGTCTCn'nnnn_
Cuts at: 0 949 1455
Size: 949 506
Bsp1286I G_dGCh'C
Cuts at: 0 77 186 701 977 1455
Size: 77 109 515 276 478
Fragments arranged by size:
515 478 276 109 77
BspHI T'CATG_A
Cuts at: 0 435 1455
Size: 435 1020
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 1455
Size: 212 1243
BsrFI r'CCGG_y
Cuts at: 0 1067 1248 1455
Size: 1067 181 207
Fragments arranged by size:
1067 207 181
BstEII G'GTnAC_C
Cuts at: 0 283 1455
Size: 283 1172
BstXI CCAn_nnnn'nTGG
Cuts at: 0 15 1455
Size: 15 1440
BstYI r'GATC_y
Cuts at: 0 3 134 887 921 1455
Size: 3 131 753 34 534
Fragments arranged by size:
753 534 131 34 3
Bsu36I CC'TnA_GG
Cuts at: 0 67 1062 1455
Size: 67 995 393
Fragments arranged by size:
995 393 67
BtgI C'CryG_G
Cuts at: 0 300 1455
Size: 300 1155
BtsI GCAGTG_nn'
Cuts at: 0 1415 1455
Size: 1415 40
EaeI y'GGCC_r
Cuts at: 0 93 224 638 1427 1455
Size: 93 131 414 789 28
Fragments arranged by size:
789 414 131 93 28
EarI CTCTTCn'nnn_
Cuts at: 0 464 1455
Size: 464 991
EcoICRI GAG'CTC
Cuts at: 0 184 699 1455
Size: 184 515 756
Fragments arranged by size:
756 515 184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 334 475 700 868 913 1455
Size: 298 36 141 225 168 45 542
Fragments arranged by size:
542 298 225 168 141 45 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 66 1455
Size: 66 1389
EcoO109I rG'GnC_Cy
Cuts at: 0 441 717 1110 1455
Size: 441 276 393 345
Fragments arranged by size:
441 393 345 276
HaeII r_GCGC'y
Cuts at: 0 300 1371 1455
Size: 300 1071 84
Fragments arranged by size:
1071 300 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 173 205 1312 1344 1455
Size: 173 32 1107 32 111
Fragments arranged by size:
1107 173 111 32 32
HindIII A'AGCT_T
Cuts at: 0 481 1455
Size: 481 974
KasI G'GCGC_C
Cuts at: 0 1367 1455
Size: 1367 88
MscI TGG'CCA
Cuts at: 0 95 226 640 1429 1455
Size: 95 131 414 789 26
Fragments arranged by size:
789 414 131 95 26
MslI CAynn'nnrTG
Cuts at: 0 55 1455
Size: 55 1400
MspA1I CmG'CkG
Cuts at: 0 113 222 612 651 661 770 907 1455
Size: 113 109 390 39 10 109 137 548
Fragments arranged by size:
548 390 137 113 109 109 39 10
NaeI GCC'GGC
Cuts at: 0 1069 1455
Size: 1069 386
NarI GG'CG_CC
Cuts at: 0 1368 1455
Size: 1368 87
NcoI C'CATG_G
Cuts at: 0 300 1455
Size: 300 1155
NgoMIV G'CCGG_C
Cuts at: 0 1067 1455
Size: 1067 388
PasI CC'CwG_GG
Cuts at: 0 1363 1455
Size: 1363 92
PfoI T'CCnGG_A
Cuts at: 0 264 1455
Size: 264 1191
PpuMI rG'GwC_Cy
Cuts at: 0 717 1455
Size: 717 738
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 145 177 1455
Size: 145 32 1278
Fragments arranged by size:
1278 145 32
PstI C_TGCA'G
Cuts at: 0 361 1455
Size: 361 1094
PvuII CAG'CTG
Cuts at: 0 113 222 612 907 1455
Size: 113 109 390 295 548
Fragments arranged by size:
548 390 295 113 109
SacI G_AGCT'C
Cuts at: 0 186 701 1455
Size: 186 515 754
Fragments arranged by size:
754 515 186
SfcI C'TryA_G
Cuts at: 0 357 646 1395 1455
Size: 357 289 749 60
Fragments arranged by size:
749 357 289 60
SfoI GGC'GCC
Cuts at: 0 1369 1455
Size: 1369 86
StuI AGG'CCT
Cuts at: 0 355 475 762 1455
Size: 355 120 287 693
Fragments arranged by size:
693 355 287 120
StyI C'CwwG_G
Cuts at: 0 14 196 300 1455
Size: 14 182 104 1155
Fragments arranged by size:
1155 182 104 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1170 1455
Size: 1170 285
TatI w'GTAC_w
Cuts at: 0 419 870 1455
Size: 419 451 585
Fragments arranged by size:
585 451 419
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 409 441 1166 1198 1262 1294 1455
Size: 409 32 725 32 64 32 161
Fragments arranged by size:
725 409 161 64 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 675 1437 1455
Size: 15 660 762 18
Fragments arranged by size:
762 660 18 15
XmnI GAAnn'nnTTC
Cuts at: 0 319 1455
Size: 319 1136
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaLI BamHI BanI BanII
BbeI BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrFI BstEII BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HindIII KasI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PfoI PpuMI PsrI PstI
PvuII SacI SfcI SfoI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsgI
BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI MmeI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI