(Linear) (Six-base) MAPSORT of: KIEE0027.seq Check: 8715 from: 1 to: 1710 KIEE0027.orf With 182 enzymes: SgfI * March 2, 2010 14:30 .. AatII G_ACGT'C Cuts at: 0 146 1710 Size: 146 1564 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 1710 Size: 235 1475 AflIII A'CryG_T Cuts at: 0 1225 1710 Size: 1225 485 AleI CACnn'nnGTG Cuts at: 0 986 1710 Size: 986 724 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 104 887 980 1710 Size: 104 783 93 730 Fragments arranged by size: 783 730 104 93 AlwNI CAG_nnn'CTG Cuts at: 0 488 690 1021 1557 1710 Size: 488 202 331 536 153 Fragments arranged by size: 536 488 331 202 153 ApaI G_GGCC'C Cuts at: 0 15 1710 Size: 15 1695 ApaLI G'TGCA_C Cuts at: 0 862 1710 Size: 862 848 AvaI C'yCGr_G Cuts at: 0 14 123 928 1710 Size: 14 109 805 782 Fragments arranged by size: 805 782 109 14 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 601 634 1710 Size: 601 33 1076 Fragments arranged by size: 1076 601 33 BanII G_rGCy'C Cuts at: 0 15 122 232 254 1710 Size: 15 107 110 22 1456 Fragments arranged by size: 1456 110 107 22 15 BbvCI CC'TCA_GC Cuts at: 0 1252 1710 Size: 1252 458 BfrBI ATG'CAT Cuts at: 0 1324 1710 Size: 1324 386 BglI GCCn_nnn'nGGC Cuts at: 0 997 1710 Size: 997 713 BlpI GC'TnA_GC Cuts at: 0 1078 1710 Size: 1078 632 Bme1580I G_kGCm'C Cuts at: 0 15 139 866 1527 1710 Size: 15 124 727 661 183 Fragments arranged by size: 727 661 183 124 15 BmrI ACTGGGnnnn_n' Cuts at: 0 1233 1343 1507 1671 1710 Size: 1233 110 164 164 39 Fragments arranged by size: 1233 164 164 110 39 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 529 1710 Size: 529 1181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 411 595 652 1031 1121 1397 1643 1710 Size: 411 184 57 379 90 276 246 67 Fragments arranged by size: 411 379 276 246 184 90 67 57 Bpu10I CC'TnA_GC Cuts at: 0 1252 1710 Size: 1252 458 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1431 1710 Size: 1431 279 BsaI GGTCTCn'nnnn_ Cuts at: 0 175 862 1568 1710 Size: 175 687 706 142 Fragments arranged by size: 706 687 175 142 BsaAI yAC'GTr Cuts at: 0 654 1710 Size: 654 1056 BsaHI Gr'CG_yC Cuts at: 0 143 1710 Size: 143 1567 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 516 546 1484 1514 1710 Size: 516 30 938 30 196 Fragments arranged by size: 938 516 196 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1190 1415 1710 Size: 1190 225 295 Fragments arranged by size: 1190 295 225 BseYI C'CCAG_C Cuts at: 0 93 1601 1710 Size: 93 1508 109 Fragments arranged by size: 1508 109 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 380 685 1531 1710 Size: 154 226 305 846 179 Fragments arranged by size: 846 305 226 179 154 BsiEI CG_ry'CG Cuts at: 0 107 1710 Size: 107 1603 BsiHKAI G_wGCw'C Cuts at: 0 26 232 866 1634 1710 Size: 26 206 634 768 76 Fragments arranged by size: 768 634 206 76 26 BsiWI C'GTAC_G Cuts at: 0 154 1710 Size: 154 1556 Bsp1286I G_dGCh'C Cuts at: 0 15 26 122 139 232 254 866 1527 Size: 15 11 96 17 93 22 612 661 Cuts at: 1527 1634 1710 Size: 107 76 Fragments arranged by size: 661 612 107 96 93 76 22 17 15 11 BspHI T'CATG_A Cuts at: 0 1382 1710 Size: 1382 328 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 556 707 962 1710 Size: 556 151 255 748 Fragments arranged by size: 748 556 255 151 BsrFI r'CCGG_y Cuts at: 0 49 1710 Size: 49 1661 BssHII G'CGCG_C Cuts at: 0 62 1710 Size: 62 1648 BssSI C'ACGA_G Cuts at: 0 859 1702 1710 Size: 859 843 8 Fragments arranged by size: 859 843 8 BstXI CCAn_nnnn'nTGG Cuts at: 0 1614 1710 Size: 1614 96 BstYI r'GATC_y Cuts at: 0 210 686 1710 Size: 210 476 1024 Fragments arranged by size: 1024 476 210 BtgI C'CryG_G Cuts at: 0 1055 1186 1710 Size: 1055 131 524 Fragments arranged by size: 1055 524 131 BtsI GCAGTG_nn' Cuts at: 0 28 981 993 1505 1710 Size: 28 953 12 512 205 Fragments arranged by size: 953 512 205 28 12 DrdI GACnn_nn'nnGTC Cuts at: 0 1197 1710 Size: 1197 513 EaeI y'GGCC_r Cuts at: 0 174 585 1568 1710 Size: 174 411 983 142 Fragments arranged by size: 983 411 174 142 EarI CTCTTCn'nnn_ Cuts at: 0 620 1447 1542 1710 Size: 620 827 95 168 Fragments arranged by size: 827 620 168 95 EcoICRI GAG'CTC Cuts at: 0 230 1710 Size: 230 1480 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 411 595 652 1031 1121 1397 1643 Size: 235 176 184 57 379 90 276 246 Cuts at: 1643 1710 Size: 67 Fragments arranged by size: 379 276 246 235 184 176 90 67 57 EcoNI CCTnn'n_nnAGG Cuts at: 0 238 1710 Size: 238 1472 EcoO109I rG'GnC_Cy Cuts at: 0 11 783 1710 Size: 11 772 927 Fragments arranged by size: 927 772 11 HaeII r_GCGC'y Cuts at: 0 1602 1710 Size: 1602 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 188 220 697 729 1103 1135 1710 Size: 188 32 477 32 374 32 575 Fragments arranged by size: 575 477 374 188 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 251 1710 Size: 251 1459 MscI TGG'CCA Cuts at: 0 587 1570 1710 Size: 587 983 140 Fragments arranged by size: 983 587 140 MslI CAynn'nnrTG Cuts at: 0 205 532 962 986 1513 1710 Size: 205 327 430 24 527 197 Fragments arranged by size: 527 430 327 205 197 24 NcoI C'CATG_G Cuts at: 0 1055 1186 1710 Size: 1055 131 524 Fragments arranged by size: 1055 524 131 NsiI A_TGCA'T Cuts at: 0 1326 1710 Size: 1326 384 NspI r_CATG'y Cuts at: 0 1077 1208 1274 1328 1710 Size: 1077 131 66 54 382 Fragments arranged by size: 1077 382 131 66 54 PasI CC'CwG_GG Cuts at: 0 1294 1710 Size: 1294 416 PflMI CCAn_nnn'nTGG Cuts at: 0 1187 1245 1519 1557 1710 Size: 1187 58 274 38 153 Fragments arranged by size: 1187 274 153 58 38 PspOMI G'GGCC_C Cuts at: 0 11 1710 Size: 11 1699 PstI C_TGCA'G Cuts at: 0 35 988 1417 1456 1658 1710 Size: 35 953 429 39 202 52 Fragments arranged by size: 953 429 202 52 39 35 SacI G_AGCT'C Cuts at: 0 232 1710 Size: 232 1478 SapI GCTCTTCn'nnn_ Cuts at: 0 620 1710 Size: 620 1090 SbfI CC_TGCA'GG Cuts at: 0 1417 1710 Size: 1417 293 SexAI A'CCwGG_T Cuts at: 0 792 1710 Size: 792 918 SfcI C'TryA_G Cuts at: 0 31 255 467 984 1001 1413 1452 1654 Size: 31 224 212 517 17 412 39 202 Cuts at: 1654 1710 Size: 56 Fragments arranged by size: 517 412 224 212 202 56 39 31 17 SmaI CCC'GGG Cuts at: 0 16 125 1710 Size: 16 109 1585 Fragments arranged by size: 1585 109 16 SmlI C'TyrA_G Cuts at: 0 1446 1710 Size: 1446 264 SphI G_CATG'C Cuts at: 0 1274 1710 Size: 1274 436 SspI AAT'ATT Cuts at: 0 757 1710 Size: 757 953 StyI C'CwwG_G Cuts at: 0 113 588 902 1055 1130 1186 1487 1565 Size: 113 475 314 153 75 56 301 78 Cuts at: 1565 1710 Size: 145 Fragments arranged by size: 475 314 301 153 145 113 78 75 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 121 895 1710 Size: 121 774 815 Fragments arranged by size: 815 774 121 TatI w'GTAC_w Cuts at: 0 545 1710 Size: 545 1165 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1484 1516 1710 Size: 1484 32 194 Fragments arranged by size: 1484 194 32 Tth111I GACn'n_nGTC Cuts at: 0 1015 1710 Size: 1015 695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1062 1710 Size: 1062 648 XmaI C'CCGG_G Cuts at: 0 14 123 1710 Size: 14 109 1587 Fragments arranged by size: 1587 109 14 ZraI GAC'GTC Cuts at: 0 144 1710 Size: 144 1566 Enzymes that do cut: AatII AcuI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BaeI BanII BbvCI BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspHI BspMI BsrFI BssHII BssSI BstXI BstYI BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MscI MslI NcoI NsiI NspI PasI PflMI PspOMI PstI SacI SapI SbfI SexAI SfcI SmaI SmlI SphI SspI StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BamHI BanI BbeI BbsI BcgI BciVI BclI BglII BmgBI BmtI BsaBI BsaWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI StuI SwaI XbaI XhoI XmnI