Restriction map for
pF1KE0028
(Linear) (Six-base) MAPSORT of: KIEE0028.seq Check: 2893 from: 1 to: 2241
KIEE0028.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 150 2241
Size: 150 2091
AatII G_ACGT'C
Cuts at: 0 916 2241
Size: 916 1325
AccI GT'mk_AC
Cuts at: 0 290 1820 2241
Size: 290 1530 421
Fragments arranged by size:
1530 421 290
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 646 2241
Size: 201 445 1595
Fragments arranged by size:
1595 445 201
AflIII A'CryG_T
Cuts at: 0 2047 2241
Size: 2047 194
AhdI GACnn_n'nnGTC
Cuts at: 0 912 2241
Size: 912 1329
AleI CACnn'nnGTG
Cuts at: 0 1904 2241
Size: 1904 337
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1642 2241
Size: 1642 599
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 100 132 1864 1896 2241
Size: 100 32 1732 32 345
Fragments arranged by size:
1732 345 100 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 517 1087 1153 2241
Size: 517 570 66 1088
Fragments arranged by size:
1088 570 517 66
ApaI G_GGCC'C
Cuts at: 0 844 1806 1954 2241
Size: 844 962 148 287
Fragments arranged by size:
962 844 287 148
AvaI C'yCGr_G
Cuts at: 0 1133 1174 2241
Size: 1133 41 1067
Fragments arranged by size:
1133 1067 41
AvrII C'CTAG_G
Cuts at: 0 2118 2241
Size: 2118 123
BamHI G'GATC_C
Cuts at: 0 1674 2241
Size: 1674 567
BanI G'GyrC_C
Cuts at: 0 892 992 2241
Size: 892 100 1249
Fragments arranged by size:
1249 892 100
BanII G_rGCy'C
Cuts at: 0 817 844 862 1094 1106 1806 1954 2136
Size: 817 27 18 232 12 700 148 182
Cuts at: 2136 2241
Size: 105
Fragments arranged by size:
817 700 232 182 148 105 27 18
12
BbeI G_GCGC'C
Cuts at: 0 996 2241
Size: 996 1245
BbvCI CC'TCA_GC
Cuts at: 0 943 2241
Size: 943 1298
BciVI GTATCCnnnnn_n'
Cuts at: 0 1929 1963 2241
Size: 1929 34 278
Fragments arranged by size:
1929 278 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2123 2241
Size: 2123 118
BlpI GC'TnA_GC
Cuts at: 0 1088 2241
Size: 1088 1153
Bme1580I G_kGCm'C
Cuts at: 0 844 895 1806 1954 2241
Size: 844 51 911 148 287
Fragments arranged by size:
911 844 287 148 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 756 968 1041 2241
Size: 756 212 73 1200
Fragments arranged by size:
1200 756 212 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1047 1839 2241
Size: 1047 792 402
Fragments arranged by size:
1047 792 402
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1645 1818 2122 2175 2241
Size: 340 1305 173 304 53 66
Fragments arranged by size:
1305 340 304 173 66 53
Bpu10I CC'TnA_GC
Cuts at: 0 323 487 836 943 2241
Size: 323 164 349 107 1298
Fragments arranged by size:
1298 349 323 164 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 1897 2133 2241
Size: 804 1093 236 108
Fragments arranged by size:
1093 804 236 108
BsaHI Gr'CG_yC
Cuts at: 0 86 913 993 1751 2066 2241
Size: 86 827 80 758 315 175
Fragments arranged by size:
827 758 315 175 86 80
BsaWI w'CCGG_w
Cuts at: 0 916 967 2241
Size: 916 51 1274
Fragments arranged by size:
1274 916 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 100 130 2241
Size: 100 30 2111
Fragments arranged by size:
2111 100 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 836 931 1178 1219 1275 2241
Size: 649 187 95 247 41 56 966
Fragments arranged by size:
966 649 247 187 95 56 41
BseYI C'CCAG_C
Cuts at: 0 1194 1401 2079 2241
Size: 1194 207 678 162
Fragments arranged by size:
1194 678 207 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 756 1073 1527 2241
Size: 460 296 317 454 714
Fragments arranged by size:
714 460 454 317 296
BsiHKAI G_wGCw'C
Cuts at: 0 862 1106 2161 2241
Size: 862 244 1055 80
Fragments arranged by size:
1055 862 244 80
BsmI GAATG_Cn'
Cuts at: 0 753 2241
Size: 753 1488
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1432 2241
Size: 1432 809
Bsp1286I G_dGCh'C
Cuts at: 0 817 844 862 895 1094 1106 1806 1954
Size: 817 27 18 33 199 12 700 148
Cuts at: 1954 2136 2161 2241
Size: 182 25 80
Fragments arranged by size:
817 700 199 182 148 80 33 27
25 18 12
BspEI T'CCGG_A
Cuts at: 0 916 2241
Size: 916 1325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 53 150 1733 1977 2188 2241
Size: 53 97 1583 244 211 53
Fragments arranged by size:
1583 244 211 97 53 53
BsrDI GCAATG_nn'
Cuts at: 0 601 1685 2241
Size: 601 1084 556
Fragments arranged by size:
1084 601 556
BsrFI r'CCGG_y
Cuts at: 0 983 2241
Size: 983 1258
BsrGI T'GTAC_A
Cuts at: 0 1699 1856 2241
Size: 1699 157 385
Fragments arranged by size:
1699 385 157
BssSI C'ACGA_G
Cuts at: 0 770 2241
Size: 770 1471
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 156 2241
Size: 72 84 2085
Fragments arranged by size:
2085 84 72
BstEII G'GTnAC_C
Cuts at: 0 2142 2241
Size: 2142 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1906 2226 2241
Size: 1906 320 15
Fragments arranged by size:
1906 320 15
BstYI r'GATC_y
Cuts at: 0 1674 2241
Size: 1674 567
Bsu36I CC'TnA_GG
Cuts at: 0 1154 2241
Size: 1154 1087
BtgI C'CryG_G
Cuts at: 0 11 54 347 728 1005 1946 2147 2165
Size: 11 43 293 381 277 941 201 18
Cuts at: 2165 2195 2241
Size: 30 46
Fragments arranged by size:
941 381 293 277 201 46 43 30
18 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1804 2102 2241
Size: 1804 298 139
Fragments arranged by size:
1804 298 139
BtsI GCAGTG_nn'
Cuts at: 0 70 1236 1413 2159 2241
Size: 70 1166 177 746 82
Fragments arranged by size:
1166 746 177 82 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2064 2099 2241
Size: 2064 35 142
Fragments arranged by size:
2064 142 35
DraIII CAC_nnn'GTG
Cuts at: 0 772 2241
Size: 772 1469
DrdI GACnn_nn'nnGTC
Cuts at: 0 31 2241
Size: 31 2210
EaeI y'GGCC_r
Cuts at: 0 863 953 2124 2241
Size: 863 90 1171 117
Fragments arranged by size:
1171 863 117 90
EarI CTCTTCn'nnn_
Cuts at: 0 55 220 644 650 805 928 2017 2241
Size: 55 165 424 6 155 123 1089 224
Fragments arranged by size:
1089 424 224 165 155 123 55 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 115 1159 2241
Size: 19 96 1044 1082
Fragments arranged by size:
1082 1044 96 19
EcoICRI GAG'CTC
Cuts at: 0 860 1104 2241
Size: 860 244 1137
Fragments arranged by size:
1137 860 244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 340 646 1645 1818 2122 2175 2241
Size: 201 139 306 999 173 304 53 66
Fragments arranged by size:
999 306 304 201 173 139 66 53
EcoO109I rG'GnC_Cy
Cuts at: 0 840 841 906 1150 1520 1802 1951 2177
Size: 840 1 65 244 370 282 149 226
Cuts at: 2177 2241
Size: 64
Fragments arranged by size:
840 370 282 244 226 149 65 64
1
HaeII r_GCGC'y
Cuts at: 0 996 2241
Size: 996 1245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 233 265 898 930 2241
Size: 233 32 633 32 1311
Fragments arranged by size:
1311 633 233 32 32
HindIII A'AGCT_T
Cuts at: 0 592 2241
Size: 592 1649
KasI G'GCGC_C
Cuts at: 0 992 2241
Size: 992 1249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 693 719 2241
Size: 693 26 1522
Fragments arranged by size:
1522 693 26
MscI TGG'CCA
Cuts at: 0 955 2126 2241
Size: 955 1171 115
Fragments arranged by size:
1171 955 115
MslI CAynn'nnrTG
Cuts at: 0 1297 1904 2164 2241
Size: 1297 607 260 77
Fragments arranged by size:
1297 607 260 77
MspA1I CmG'CkG
Cuts at: 0 56 81 144 947 990 1356 1744 1976
Size: 56 25 63 803 43 366 388 232
Cuts at: 1976 2241
Size: 265
Fragments arranged by size:
803 388 366 265 232 63 56 43
25
NarI GG'CG_CC
Cuts at: 0 993 2241
Size: 993 1248
NcoI C'CATG_G
Cuts at: 0 728 1005 2165 2195 2241
Size: 728 277 1160 30 46
Fragments arranged by size:
1160 728 277 46 30
NspI r_CATG'y
Cuts at: 0 510 1485 1773 2043 2051 2241
Size: 510 975 288 270 8 190
Fragments arranged by size:
975 510 288 270 190 8
PasI CC'CwG_GG
Cuts at: 0 2000 2241
Size: 2000 241
PciI A'CATG_T
Cuts at: 0 2047 2241
Size: 2047 194
PfoI T'CCnGG_A
Cuts at: 0 35 2241
Size: 35 2206
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 2241
Size: 100 32 2109
Fragments arranged by size:
2109 100 32
PpuMI rG'GwC_Cy
Cuts at: 0 906 2177 2241
Size: 906 1271 64
Fragments arranged by size:
1271 906 64
PspOMI G'GGCC_C
Cuts at: 0 840 1802 1950 2241
Size: 840 962 148 291
Fragments arranged by size:
962 840 291 148
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1840 1872 2241
Size: 1840 32 369
Fragments arranged by size:
1840 369 32
PstI C_TGCA'G
Cuts at: 0 149 1001 1243 2241
Size: 149 852 242 998
Fragments arranged by size:
998 852 242 149
PvuII CAG'CTG
Cuts at: 0 81 144 1744 2241
Size: 81 63 1600 497
Fragments arranged by size:
1600 497 81 63
SacI G_AGCT'C
Cuts at: 0 862 1106 2241
Size: 862 244 1135
Fragments arranged by size:
1135 862 244
SacII CC_GC'GG
Cuts at: 0 57 2241
Size: 57 2184
SapI GCTCTTCn'nnn_
Cuts at: 0 55 220 805 928 2241
Size: 55 165 585 123 1313
Fragments arranged by size:
1313 585 165 123 55
SbfI CC_TGCA'GG
Cuts at: 0 1001 2241
Size: 1001 1240
SexAI A'CCwGG_T
Cuts at: 0 557 2241
Size: 557 1684
SfcI C'TryA_G
Cuts at: 0 145 997 1239 2241
Size: 145 852 242 1002
Fragments arranged by size:
1002 852 242 145
SfoI GGC'GCC
Cuts at: 0 994 2241
Size: 994 1247
SmlI C'TyrA_G
Cuts at: 0 819 1912 2112 2241
Size: 819 1093 200 129
Fragments arranged by size:
1093 819 200 129
SphI G_CATG'C
Cuts at: 0 510 2241
Size: 510 1731
StuI AGG'CCT
Cuts at: 0 661 2241
Size: 661 1580
StyI C'CwwG_G
Cuts at: 0 662 728 896 923 1005 2118 2165 2195
Size: 662 66 168 27 82 1113 47 30
Cuts at: 2195 2241
Size: 46
Fragments arranged by size:
1113 662 168 82 66 47 46 30
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2071 2241
Size: 2071 170
TatI w'GTAC_w
Cuts at: 0 1699 1856 1925 2241
Size: 1699 157 69 316
Fragments arranged by size:
1699 316 157 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1210 1242 2241
Size: 1210 32 999
Fragments arranged by size:
1210 999 32
XmnI GAAnn'nnTTC
Cuts at: 0 115 2241
Size: 115 2126
ZraI GAC'GTC
Cuts at: 0 914 2241
Size: 914 1327
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI AvaI AvrII BamHI BanI BanII
BbeI BbvCI BciVI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HindIII KasI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PciI PfoI PpiI PpuMI PspOMI PsrI PstI PvuII
SacI SacII SapI SbfI SexAI SfcI SfoI SmlI
SphI StuI StyI TaqII TatI TstI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI ApaLI ApoI AscI
AseI AsiSI BaeI BbsI BcgI BclI BfrBI BglII
BmgBI BmtI BsaI BsaAI BsaBI BsiEI BsiWI BspHI
BsrBI BssHII BstBI BstZ17I ClaI DraI EagI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI
KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflMI PmeI PmlI PshAI PsiI
PspXI PvuI RsrII SalI SanDI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XcmI XhoI XmaI