Restriction map for
pF1KE0038
(Linear) (Six-base) MAPSORT of: KIEE0038.seq Check: 4522 from: 1 to: 2106
KIEE0038.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 1871 2106
Size: 1871 235
Acc65I G'GTAC_C
Cuts at: 0 1654 2106
Size: 1654 452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 2106
Size: 29 2077
AflIII A'CryG_T
Cuts at: 0 506 1792 2068 2106
Size: 506 1286 276 38
Fragments arranged by size:
1286 506 276 38
AleI CACnn'nnGTG
Cuts at: 0 507 1562 2106
Size: 507 1055 544
Fragments arranged by size:
1055 544 507
ApoI r'AATT_y
Cuts at: 0 146 228 399 872 1195 1225 1597 1751
Size: 146 82 171 473 323 30 372 154
Cuts at: 1751 1948 2014 2106
Size: 197 66 92
Fragments arranged by size:
473 372 323 197 171 154 146 92
82 66 30
AseI AT'TA_AT
Cuts at: 0 386 431 2106
Size: 386 45 1675
Fragments arranged by size:
1675 386 45
BanI G'GyrC_C
Cuts at: 0 1654 2106
Size: 1654 452
BanII G_rGCy'C
Cuts at: 0 1779 2106
Size: 1779 327
BbsI GAAGACnn'nnnn_
Cuts at: 0 513 2106
Size: 513 1593
BglII A'GATC_T
Cuts at: 0 720 2106
Size: 720 1386
BmrI ACTGGGnnnn_n'
Cuts at: 0 685 2106
Size: 685 1421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1519 2106
Size: 1519 587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1458 2106
Size: 1458 648
BsaI GGTCTCn'nnnn_
Cuts at: 0 1537 2106
Size: 1537 569
BsaAI yAC'GTr
Cuts at: 0 702 2106
Size: 702 1404
BsaBI GATnn'nnATC
Cuts at: 0 914 1467 2106
Size: 914 553 639
Fragments arranged by size:
914 639 553
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1081 1111 2106
Size: 1081 30 995
Fragments arranged by size:
1081 995 30
BseYI C'CCAG_C
Cuts at: 0 1902 2106
Size: 1902 204
BsiHKAI G_wGCw'C
Cuts at: 0 730 1827 2106
Size: 730 1097 279
Fragments arranged by size:
1097 730 279
BsmI GAATG_Cn'
Cuts at: 0 642 1573 2106
Size: 642 931 533
Fragments arranged by size:
931 642 533
Bsp1286I G_dGCh'C
Cuts at: 0 730 1779 1827 2106
Size: 730 1049 48 279
Fragments arranged by size:
1049 730 279 48
BsrDI GCAATG_nn'
Cuts at: 0 78 883 1718 2106
Size: 78 805 835 388
Fragments arranged by size:
835 805 388 78
BsrFI r'CCGG_y
Cuts at: 0 1306 2106
Size: 1306 800
BsrGI T'GTAC_A
Cuts at: 0 1588 2106
Size: 1588 518
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1117 2106
Size: 1117 989
BstYI r'GATC_y
Cuts at: 0 262 512 679 720 2106
Size: 262 250 167 41 1386
Fragments arranged by size:
1386 262 250 167 41
Bsu36I CC'TnA_GG
Cuts at: 0 746 2106
Size: 746 1360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1800 1835 2106
Size: 1800 35 271
Fragments arranged by size:
1800 271 35
DraI TTT'AAA
Cuts at: 0 1080 2106
Size: 1080 1026
DraIII CAC_nnn'GTG
Cuts at: 0 130 2106
Size: 130 1976
EarI CTCTTCn'nnn_
Cuts at: 0 192 2106
Size: 192 1914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 1519 2106
Size: 29 1490 587
Fragments arranged by size:
1490 587 29
EcoRI G'AATT_C
Cuts at: 0 1751 2014 2106
Size: 1751 263 92
Fragments arranged by size:
1751 263 92
EcoRV GAT'ATC
Cuts at: 0 1250 2106
Size: 1250 856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 105 2106
Size: 105 2001
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 169 201 1169 1201 2106
Size: 25 32 112 32 968 32 905
Fragments arranged by size:
968 905 112 32 32 32 25
HincII GTy'rAC
Cuts at: 0 1673 2106
Size: 1673 433
HindIII A'AGCT_T
Cuts at: 0 213 2106
Size: 213 1893
KpnI G_GTAC'C
Cuts at: 0 1658 2106
Size: 1658 448
MfeI C'AATT_G
Cuts at: 0 1389 2106
Size: 1389 717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 744 1194 2106
Size: 744 450 912
Fragments arranged by size:
912 744 450
MslI CAynn'nnrTG
Cuts at: 0 507 1355 1562 2106
Size: 507 848 207 544
Fragments arranged by size:
848 544 507 207
MspA1I CmG'CkG
Cuts at: 0 1817 2106
Size: 1817 289
NaeI GCC'GGC
Cuts at: 0 1308 2106
Size: 1308 798
NgoMIV G'CCGG_C
Cuts at: 0 1306 2106
Size: 1306 800
NspI r_CATG'y
Cuts at: 0 510 892 1796 2072 2085 2106
Size: 510 382 904 276 13 21
Fragments arranged by size:
904 510 382 276 21 13
PacI TTA_AT'TAA
Cuts at: 0 431 2106
Size: 431 1675
PciI A'CATG_T
Cuts at: 0 506 1792 2068 2106
Size: 506 1286 276 38
Fragments arranged by size:
1286 506 276 38
PflMI CCAn_nnn'nTGG
Cuts at: 0 664 1420 1728 2106
Size: 664 756 308 378
Fragments arranged by size:
756 664 378 308
PsiI TTA'TAA
Cuts at: 0 1505 1735 2106
Size: 1505 230 371
Fragments arranged by size:
1505 371 230
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1531 1563 2024 2056 2106
Size: 1531 32 461 32 50
Fragments arranged by size:
1531 461 50 32 32
PvuII CAG'CTG
Cuts at: 0 1817 2106
Size: 1817 289
SfcI C'TryA_G
Cuts at: 0 1509 1886 2106
Size: 1509 377 220
Fragments arranged by size:
1509 377 220
SmlI C'TyrA_G
Cuts at: 0 1473 2106
Size: 1473 633
StyI C'CwwG_G
Cuts at: 0 939 1967 2106
Size: 939 1028 139
Fragments arranged by size:
1028 939 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 294 1552 2106
Size: 294 1258 554
Fragments arranged by size:
1258 554 294
TatI w'GTAC_w
Cuts at: 0 1588 2106
Size: 1588 518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 567 2106
Size: 567 1539
XmnI GAAnn'nnTTC
Cuts at: 0 873 1335 1600 2106
Size: 873 462 265 506
Fragments arranged by size:
873 506 462 265
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI ApoI AseI BanI
BanII BbsI BglII BmrI BpmI BpuEI BsaI BsaAI
BsaBI BsaXI BseYI BsiHKAI BsmI Bsp1286I BsrDI BsrFI
BsrGI BstAPI BstYI Bsu36I CspCI DraI DraIII EarI
Eco57MI EcoRI EcoRV FalI Hin4I HincII HindIII KpnI
MfeI MmeI MslI MspA1I NaeI NgoMIV NspI PacI
PciI PflMI PsiI PsrI PvuII SfcI SmlI StyI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaHI
BsaWI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgI BtgZI BtsI ClaI DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I FseI FspI FspAI HaeII HpaI
KasI MluI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PasI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmaI ZraI