Restriction map for
pF1KE0039
(Linear) (Six-base) MAPSORT of: KIEE0039.seq Check: 1057 from: 1 to: 1539
KIEE0039.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AflIII A'CryG_T
Cuts at: 0 1113 1539
Size: 1113 426
AleI CACnn'nnGTG
Cuts at: 0 267 1539
Size: 267 1272
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1210 1242 1539
Size: 1210 32 297
Fragments arranged by size:
1210 297 32
AlwNI CAG_nnn'CTG
Cuts at: 0 223 1539
Size: 223 1316
ApoI r'AATT_y
Cuts at: 0 185 433 649 1539
Size: 185 248 216 890
Fragments arranged by size:
890 248 216 185
AseI AT'TA_AT
Cuts at: 0 949 1539
Size: 949 590
AvaI C'yCGr_G
Cuts at: 0 485 1539
Size: 485 1054
BamHI G'GATC_C
Cuts at: 0 723 1539
Size: 723 816
BanI G'GyrC_C
Cuts at: 0 276 902 1539
Size: 276 626 637
Fragments arranged by size:
637 626 276
BanII G_rGCy'C
Cuts at: 0 671 1539
Size: 671 868
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 1539
Size: 265 1274
BciVI GTATCCnnnnn_n'
Cuts at: 0 1276 1539
Size: 1276 263
BclI T'GATC_A
Cuts at: 0 684 1049 1539
Size: 684 365 490
Fragments arranged by size:
684 490 365
BglI GCCn_nnn'nGGC
Cuts at: 0 296 1539
Size: 296 1243
BlpI GC'TnA_GC
Cuts at: 0 803 1539
Size: 803 736
BmgBI CAC'GTC
Cuts at: 0 1114 1539
Size: 1114 425
BmrI ACTGGGnnnn_n'
Cuts at: 0 673 1539
Size: 673 866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1209 1539
Size: 1209 330
Bpu10I CC'TnA_GC
Cuts at: 0 98 1539
Size: 98 1441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1539
Size: 268 1271
BsaWI w'CCGG_w
Cuts at: 0 62 357 1539
Size: 62 295 1182
Fragments arranged by size:
1182 295 62
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1401 1539
Size: 1401 138
Bsp1286I G_dGCh'C
Cuts at: 0 671 1539
Size: 671 868
BspEI T'CCGG_A
Cuts at: 0 62 357 1539
Size: 62 295 1182
Fragments arranged by size:
1182 295 62
BspHI T'CATG_A
Cuts at: 0 254 467 645 1367 1539
Size: 254 213 178 722 172
Fragments arranged by size:
722 254 213 178 172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 807 1539
Size: 807 732
BssSI C'ACGA_G
Cuts at: 0 368 1539
Size: 368 1171
BstAPI GCAn_nnn'nTGC
Cuts at: 0 373 1184 1539
Size: 373 811 355
Fragments arranged by size:
811 373 355
BstYI r'GATC_y
Cuts at: 0 723 914 1539
Size: 723 191 625
Fragments arranged by size:
723 625 191
Bsu36I CC'TnA_GG
Cuts at: 0 791 1539
Size: 791 748
BtgI C'CryG_G
Cuts at: 0 492 1214 1314 1539
Size: 492 722 100 225
Fragments arranged by size:
722 492 225 100
DraI TTT'AAA
Cuts at: 0 894 1029 1539
Size: 894 135 510
Fragments arranged by size:
894 510 135
EaeI y'GGCC_r
Cuts at: 0 537 964 1539
Size: 537 427 575
Fragments arranged by size:
575 537 427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1209 1539
Size: 1209 330
EcoRV GAT'ATC
Cuts at: 0 756 927 1428 1539
Size: 756 171 501 111
Fragments arranged by size:
756 501 171 111
HaeII r_GCGC'y
Cuts at: 0 9 1539
Size: 9 1530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 1455 1487 1539
Size: 1049 32 374 32 52
Fragments arranged by size:
1049 374 52 32 32
HindIII A'AGCT_T
Cuts at: 0 1485 1539
Size: 1485 54
MscI TGG'CCA
Cuts at: 0 539 966 1539
Size: 539 427 573
Fragments arranged by size:
573 539 427
MslI CAynn'nnrTG
Cuts at: 0 267 584 680 833 1539
Size: 267 317 96 153 706
Fragments arranged by size:
706 317 267 153 96
MspA1I CmG'CkG
Cuts at: 0 979 1539
Size: 979 560
NcoI C'CATG_G
Cuts at: 0 492 1214 1539
Size: 492 722 325
Fragments arranged by size:
722 492 325
PasI CC'CwG_GG
Cuts at: 0 310 1539
Size: 310 1229
PflMI CCAn_nnn'nTGG
Cuts at: 0 1215 1539
Size: 1215 324
PfoI T'CCnGG_A
Cuts at: 0 1107 1254 1539
Size: 1107 147 285
Fragments arranged by size:
1107 285 147
PshAI GACnn'nnGTC
Cuts at: 0 487 1539
Size: 487 1052
PsiI TTA'TAA
Cuts at: 0 34 1539
Size: 34 1505
PspXI vC'TCGA_Gb
Cuts at: 0 485 1539
Size: 485 1054
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 806 838 1539
Size: 806 32 701
Fragments arranged by size:
806 701 32
PvuII CAG'CTG
Cuts at: 0 979 1539
Size: 979 560
SfcI C'TryA_G
Cuts at: 0 336 1539
Size: 336 1203
SmlI C'TyrA_G
Cuts at: 0 247 485 1539
Size: 247 238 1054
Fragments arranged by size:
1054 247 238
SspI AAT'ATT
Cuts at: 0 1356 1539
Size: 1356 183
StyI C'CwwG_G
Cuts at: 0 492 524 605 1214 1539
Size: 492 32 81 609 325
Fragments arranged by size:
609 492 325 81 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 279 302 556 1539
Size: 279 23 254 983
Fragments arranged by size:
983 279 254 23
TatI w'GTAC_w
Cuts at: 0 1289 1539
Size: 1289 250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 203 235 1539
Size: 203 32 1304
Fragments arranged by size:
1304 203 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 775 1539
Size: 775 764
XhoI C'TCGA_G
Cuts at: 0 485 1539
Size: 485 1054
Enzymes that do cut:
AflIII AleI AloI AlwNI ApoI AseI AvaI BamHI
BanI BanII BbsI BciVI BclI BglI BlpI BmgBI
BmrI BpmI Bpu10I BpuEI BsaWI BseRI Bsp1286I BspEI
BspHI BspMI BssSI BstAPI BstYI Bsu36I BtgI DraI
EaeI Eco57MI EcoRV HaeII Hin4I HindIII MscI MslI
MspA1I NcoI PasI PflMI PfoI PshAI PsiI PspXI
PsrI PvuII SfcI SmlI SspI StyI TaqII TatI
TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI ApaI ApaLI AscI AsiSI AvrII
BaeI BbeI BbvCI BcgI BfrBI BglII Bme1580I BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI
BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XmaI XmnI
ZraI