Restriction map for
pF1KE0050
(Linear) (Six-base) MAPSORT of: KIEE0050.seq Check: 2463 from: 1 to: 1407
KIEE0050.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 0 492 1373 1407
Size: 492 881 34
Fragments arranged by size:
881 492 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1335 1407
Size: 329 1006 72
Fragments arranged by size:
1006 329 72
AfeI AGC'GCT
Cuts at: 0 713 1407
Size: 713 694
AflIII A'CryG_T
Cuts at: 0 907 1407
Size: 907 500
AhdI GACnn_n'nnGTC
Cuts at: 0 680 1268 1352 1407
Size: 680 588 84 55
Fragments arranged by size:
680 588 84 55
AleI CACnn'nnGTG
Cuts at: 0 638 1407
Size: 638 769
ApoI r'AATT_y
Cuts at: 0 91 377 1407
Size: 91 286 1030
Fragments arranged by size:
1030 286 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 13 1407
Size: 13 1394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1248 1282 1407
Size: 1248 34 125
Fragments arranged by size:
1248 125 34
BclI T'GATC_A
Cuts at: 0 219 1407
Size: 219 1188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 827 911 995 1163 1247 1331 1407
Size: 743 84 84 84 168 84 84 76
Fragments arranged by size:
743 168 84 84 84 84 84 76
BsrDI GCAATG_nn'
Cuts at: 0 58 1407
Size: 58 1349
BstZ17I GTA'TAC
Cuts at: 0 493 1374 1407
Size: 493 881 33
Fragments arranged by size:
881 493 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1265 1407
Size: 1265 142
DrdI GACnn_nn'nnGTC
Cuts at: 0 15 74 1407
Size: 15 59 1333
Fragments arranged by size:
1333 59 15
EaeI y'GGCC_r
Cuts at: 0 162 1407
Size: 162 1245
EarI CTCTTCn'nnn_
Cuts at: 0 551 703 992 1407
Size: 551 152 289 415
Fragments arranged by size:
551 415 289 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 743 827 911 995 1163 1247 1331
Size: 329 414 84 84 84 168 84 84
Cuts at: 1331 1335 1407
Size: 4 72
Fragments arranged by size:
414 329 168 84 84 84 84 84
72 4
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 274 1407
Size: 274 1133
HaeII r_GCGC'y
Cuts at: 0 715 1407
Size: 715 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 666 698 1254 1286 1338 1370 1407
Size: 666 32 556 32 52 32 37
Fragments arranged by size:
666 556 52 37 32 32 32
HincII GTy'rAC
Cuts at: 0 20 1407
Size: 20 1387
HindIII A'AGCT_T
Cuts at: 0 267 1407
Size: 267 1140
MscI TGG'CCA
Cuts at: 0 164 1407
Size: 164 1243
MslI CAynn'nnrTG
Cuts at: 0 453 638 822 1158 1407
Size: 453 185 184 336 249
Fragments arranged by size:
453 336 249 185 184
NspI r_CATG'y
Cuts at: 0 232 911 1407
Size: 232 679 496
Fragments arranged by size:
679 496 232
PciI A'CATG_T
Cuts at: 0 907 1407
Size: 907 500
PsiI TTA'TAA
Cuts at: 0 1288 1407
Size: 1288 119
SphI G_CATG'C
Cuts at: 0 232 1407
Size: 232 1175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 1258 1407
Size: 189 1069 149
Fragments arranged by size:
1069 189 149
TatI w'GTAC_w
Cuts at: 0 205 462 1407
Size: 205 257 945
Fragments arranged by size:
945 257 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1041 1073 1407
Size: 1041 32 334
Fragments arranged by size:
1041 334 32
Tth111I GACn'n_nGTC
Cuts at: 0 12 1407
Size: 12 1395
XmnI GAAnn'nnTTC
Cuts at: 0 423 1407
Size: 423 984
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI ApoI BbsI
BcgI BclI BpmI BsrDI BstZ17I BtgZI DrdI EaeI
EarI Eco57MI FalI HaeII Hin4I HincII HindIII MscI
MslI NspI PciI PsiI SphI TaqII TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BciVI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtsI ClaI CspCI DraI DraIII EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI StyI SwaI XbaI XcmI XhoI
XmaI ZraI