Restriction map for
pF1KE0057
(Linear) (Six-base) MAPSORT of: KIEE0057.seq Check: 4008 from: 1 to: 3537
KIEE0057.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1077 3188 3537
Size: 1077 2111 349
Fragments arranged by size:
2111 1077 349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 93 398 932 1041 1426 1459 1771
Size: 28 65 305 534 109 385 33 312
Cuts at: 1771 1831 1939 3157 3453 3520 3537
Size: 60 108 1218 296 67 17
Fragments arranged by size:
1218 534 385 312 305 296 109 108
67 65 60 33 28 17
AfeI AGC'GCT
Cuts at: 0 1762 1828 3537
Size: 1762 66 1709
Fragments arranged by size:
1762 1709 66
AleI CACnn'nnGTG
Cuts at: 0 566 3537
Size: 566 2971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 655 3449 3537
Size: 655 2794 88
Fragments arranged by size:
2794 655 88
AlwNI CAG_nnn'CTG
Cuts at: 0 1282 1404 2051 2139 2212 2300 3180 3324
Size: 1282 122 647 88 73 88 880 144
Cuts at: 3324 3537
Size: 213
Fragments arranged by size:
1282 880 647 213 144 122 88 88
73
ApaI G_GGCC'C
Cuts at: 0 2606 3537
Size: 2606 931
ApoI r'AATT_y
Cuts at: 0 1285 1438 1972 3537
Size: 1285 153 534 1565
Fragments arranged by size:
1565 1285 534 153
AvaI C'yCGr_G
Cuts at: 0 938 1978 3537
Size: 938 1040 1559
Fragments arranged by size:
1559 1040 938
BamHI G'GATC_C
Cuts at: 0 481 2452 3537
Size: 481 1971 1085
Fragments arranged by size:
1971 1085 481
BanI G'GyrC_C
Cuts at: 0 932 1089 1230 2535 2595 2911 3537
Size: 932 157 141 1305 60 316 626
Fragments arranged by size:
1305 932 626 316 157 141 60
BanII G_rGCy'C
Cuts at: 0 46 378 1985 2206 2606 2635 3386 3537
Size: 46 332 1607 221 400 29 751 151
Fragments arranged by size:
1607 751 400 332 221 151 46 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 311 545 1161 1248 3537
Size: 311 234 616 87 2289
Fragments arranged by size:
2289 616 311 234 87
BbvCI CC'TCA_GC
Cuts at: 0 1529 1620 3537
Size: 1529 91 1917
Fragments arranged by size:
1917 1529 91
BciVI GTATCCnnnnn_n'
Cuts at: 0 1450 3537
Size: 1450 2087
BclI T'GATC_A
Cuts at: 0 624 3537
Size: 624 2913
BglI GCCn_nnn'nGGC
Cuts at: 0 3451 3537
Size: 3451 86
BglII A'GATC_T
Cuts at: 0 851 1043 1803 3537
Size: 851 192 760 1734
Fragments arranged by size:
1734 851 760 192
BlpI GC'TnA_GC
Cuts at: 0 818 1304 2306 3537
Size: 818 486 1002 1231
Fragments arranged by size:
1231 1002 818 486
Bme1580I G_kGCm'C
Cuts at: 0 937 2031 2598 2606 2916 3537
Size: 937 1094 567 8 310 621
Fragments arranged by size:
1094 937 621 567 310 8
BmgBI CAC'GTC
Cuts at: 0 1509 3537
Size: 1509 2028
BmrI ACTGGGnnnn_n'
Cuts at: 0 560 675 1735 3537
Size: 560 115 1060 1802
Fragments arranged by size:
1802 1060 560 115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1443 2434 3152 3537
Size: 1443 991 718 385
Fragments arranged by size:
1443 991 718 385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 787 1413 1947 2995 3047 3105 3346
Size: 485 302 626 534 1048 52 58 241
Cuts at: 3346 3537
Size: 191
Fragments arranged by size:
1048 626 534 485 302 241 191 58
52
Bpu10I CC'TnA_GC
Cuts at: 0 648 839 1529 1620 1699 2064 3083 3537
Size: 648 191 690 91 79 365 1019 454
Fragments arranged by size:
1019 690 648 454 365 191 91 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 216 2333 2954 3537
Size: 37 179 2117 621 583
Fragments arranged by size:
2117 621 583 179 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 2820 2856 3537
Size: 2820 36 681
Fragments arranged by size:
2820 681 36
BsaBI GATnn'nnATC
Cuts at: 0 1672 2451 3537
Size: 1672 779 1086
Fragments arranged by size:
1672 1086 779
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1030 1060 2469 2499 3537
Size: 1030 30 1409 30 1038
Fragments arranged by size:
1409 1038 1030 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 194 738 1038 1771 1848 2945 3537
Size: 194 544 300 733 77 1097 592
Fragments arranged by size:
1097 733 592 544 300 194 77
BseYI C'CCAG_C
Cuts at: 0 45 1010 1210 1792 1995 2093 2456 2862
Size: 45 965 200 582 203 98 363 406
Cuts at: 2862 2991 3045 3317 3379 3537
Size: 129 54 272 62 158
Fragments arranged by size:
965 582 406 363 272 203 200 158
129 98 62 54 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 3537
Size: 946 2591
BsiEI CG_ry'CG
Cuts at: 0 1736 2844 3537
Size: 1736 1108 693
Fragments arranged by size:
1736 1108 693
BsiHKAI G_wGCw'C
Cuts at: 0 697 1985 2065 2307 3537
Size: 697 1288 80 242 1230
Fragments arranged by size:
1288 1230 697 242 80
Bsp1286I G_dGCh'C
Cuts at: 0 46 378 697 937 1985 2031 2065 2206
Size: 46 332 319 240 1048 46 34 141
Cuts at: 2206 2307 2598 2606 2635 2916 3386 3537
Size: 101 291 8 29 281 470 151
Fragments arranged by size:
1048 470 332 319 291 281 240 151
141 101 46 46 34 29 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 1077 3188 3537
Size: 191 886 2111 349
Fragments arranged by size:
2111 886 349 191
BsrBI CCG'CTC
Cuts at: 0 21 2079 3537
Size: 21 2058 1458
Fragments arranged by size:
2058 1458 21
BsrFI r'CCGG_y
Cuts at: 0 530 3537
Size: 530 3007
BstAPI GCAn_nnn'nTGC
Cuts at: 0 914 2685 3442 3537
Size: 914 1771 757 95
Fragments arranged by size:
1771 914 757 95
BstEII G'GTnAC_C
Cuts at: 0 1410 3537
Size: 1410 2127
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1782 2250 3537
Size: 1782 468 1287
Fragments arranged by size:
1782 1287 468
BstYI r'GATC_y
Cuts at: 0 447 481 588 785 851 1043 1667 1803
Size: 447 34 107 197 66 192 624 136
Cuts at: 1803 2452 3537
Size: 649 1085
Fragments arranged by size:
1085 649 624 447 197 192 136 107
66 34
BtgI C'CryG_G
Cuts at: 0 1736 2123 2747 3537
Size: 1736 387 624 790
Fragments arranged by size:
1736 790 624 387
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2539 3501 3537
Size: 2539 962 36
Fragments arranged by size:
2539 962 36
BtsI GCAGTG_nn'
Cuts at: 0 240 642 882 934 1081 2273 3443 3537
Size: 240 402 240 52 147 1192 1170 94
Fragments arranged by size:
1192 1170 402 240 240 147 94 52
DrdI GACnn_nn'nnGTC
Cuts at: 0 2834 3537
Size: 2834 703
EaeI y'GGCC_r
Cuts at: 0 22 71 1487 1733 1835 2270 2841 3537
Size: 22 49 1416 246 102 435 571 696
Fragments arranged by size:
1416 696 571 435 246 102 49 22
EagI C'GGCC_G
Cuts at: 0 1733 2841 3537
Size: 1733 1108 696
Fragments arranged by size:
1733 1108 696
EarI CTCTTCn'nnn_
Cuts at: 0 1056 1495 2732 3229 3302 3537
Size: 1056 439 1237 497 73 235
Fragments arranged by size:
1237 1056 497 439 235 73
EcoICRI GAG'CTC
Cuts at: 0 1983 3537
Size: 1983 1554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 93 398 485 787 932 1041 1413
Size: 28 65 305 87 302 145 109 372
Cuts at: 1413 1426 1459 1771 1831 1939 1947 2995 3047
Size: 13 33 312 60 108 8 1048 52
Cuts at: 3047 3105 3157 3346 3453 3520 3537
Size: 58 52 189 107 67 17
Fragments arranged by size:
1048 372 312 305 302 189 145 109
108 107 87 67 65 60 58 52
52 33 28 17 13 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 198 1702 1786 2960 3537
Size: 198 1504 84 1174 577
Fragments arranged by size:
1504 1174 577 198 84
EcoO109I rG'GnC_Cy
Cuts at: 0 153 942 2136 2188 2209 2472 2563 2603
Size: 153 789 1194 52 21 263 91 40
Cuts at: 2603 2836 2904 2965 3429 3537
Size: 233 68 61 464 108
Fragments arranged by size:
1194 789 464 263 233 153 108 91
68 61 52 40 21
EcoRI G'AATT_C
Cuts at: 0 1285 3537
Size: 1285 2252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1431 1489 2666 3115 3258 3537
Size: 1431 58 1177 449 143 279
Fragments arranged by size:
1431 1177 449 279 143 58
HaeII r_GCGC'y
Cuts at: 0 1764 1830 3253 3537
Size: 1764 66 1423 284
Fragments arranged by size:
1764 1423 284 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1030 1062 2422 2454 2709 2741 3211 3243
Size: 1030 32 1360 32 255 32 470 32
Cuts at: 3243 3537
Size: 294
Fragments arranged by size:
1360 1030 470 294 255 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1367 1447 2877 3417 3537
Size: 1367 80 1430 540 120
Fragments arranged by size:
1430 1367 540 120 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3282 3537
Size: 3282 255
MscI TGG'CCA
Cuts at: 0 73 1489 1837 2272 3537
Size: 73 1416 348 435 1265
Fragments arranged by size:
1416 1265 435 348 73
MslI CAynn'nnrTG
Cuts at: 0 566 2278 3537
Size: 566 1712 1259
Fragments arranged by size:
1712 1259 566
MspA1I CmG'CkG
Cuts at: 0 28 726 911 1303 3537
Size: 28 698 185 392 2234
Fragments arranged by size:
2234 698 392 185 28
NcoI C'CATG_G
Cuts at: 0 2123 2747 3537
Size: 2123 624 790
Fragments arranged by size:
2123 790 624
NdeI CA'TA_TG
Cuts at: 0 1025 3537
Size: 1025 2512
NspI r_CATG'y
Cuts at: 0 288 1995 2756 3445 3537
Size: 288 1707 761 689 92
Fragments arranged by size:
1707 761 689 288 92
PasI CC'CwG_GG
Cuts at: 0 477 2259 2294 2635 2713 2999 3537
Size: 477 1782 35 341 78 286 538
Fragments arranged by size:
1782 538 477 341 286 78 35
PflMI CCAn_nnn'nTGG
Cuts at: 0 2941 3324 3537
Size: 2941 383 213
Fragments arranged by size:
2941 383 213
PfoI T'CCnGG_A
Cuts at: 0 2977 3150 3537
Size: 2977 173 387
Fragments arranged by size:
2977 387 173
PpuMI rG'GwC_Cy
Cuts at: 0 153 942 2136 2188 2472 2563 2836 2904
Size: 153 789 1194 52 284 91 273 68
Cuts at: 2904 2965 3537
Size: 61 572
Fragments arranged by size:
1194 789 572 284 273 153 91 68
61 52
PspOMI G'GGCC_C
Cuts at: 0 2602 3537
Size: 2602 935
PstI C_TGCA'G
Cuts at: 0 1088 1313 1579 2135 3170 3537
Size: 1088 225 266 556 1035 367
Fragments arranged by size:
1088 1035 556 367 266 225
PvuII CAG'CTG
Cuts at: 0 28 726 911 1303 3537
Size: 28 698 185 392 2234
Fragments arranged by size:
2234 698 392 185 28
SacI G_AGCT'C
Cuts at: 0 1985 3537
Size: 1985 1552
SanDI GG'GwC_CC
Cuts at: 0 153 942 2563 2904 3537
Size: 153 789 1621 341 633
Fragments arranged by size:
1621 789 633 341 153
SapI GCTCTTCn'nnn_
Cuts at: 0 3229 3537
Size: 3229 308
SexAI A'CCwGG_T
Cuts at: 0 2468 3537
Size: 2468 1069
SfcI C'TryA_G
Cuts at: 0 1084 1309 1575 1911 2131 2223 2589 3166
Size: 1084 225 266 336 220 92 366 577
Cuts at: 3166 3459 3537
Size: 293 78
Fragments arranged by size:
1084 577 366 336 293 266 225 220
92 78
SmlI C'TyrA_G
Cuts at: 0 16 195 2348 2969 3537
Size: 16 179 2153 621 568
Fragments arranged by size:
2153 621 568 179 16
SpeI A'CTAG_T
Cuts at: 0 832 3537
Size: 832 2705
SphI G_CATG'C
Cuts at: 0 288 2756 3445 3537
Size: 288 2468 689 92
Fragments arranged by size:
2468 689 288 92
SspI AAT'ATT
Cuts at: 0 134 3537
Size: 134 3403
StuI AGG'CCT
Cuts at: 0 653 1318 1570 1585 2646 3537
Size: 653 665 252 15 1061 891
Fragments arranged by size:
1061 891 665 653 252 15
StyI C'CwwG_G
Cuts at: 0 1319 1535 1660 2123 2488 2494 2531 2747
Size: 1319 216 125 463 365 6 37 216
Cuts at: 2747 3537
Size: 790
Fragments arranged by size:
1319 790 463 365 216 216 125 37
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 694 2975 3537
Size: 694 2281 562
Fragments arranged by size:
2281 694 562
TatI w'GTAC_w
Cuts at: 0 989 3537
Size: 989 2548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1888 1920 3537
Size: 1888 32 1617
Fragments arranged by size:
1888 1617 32
XmnI GAAnn'nnTTC
Cuts at: 0 391 3537
Size: 391 3146
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HindIII MmeI MscI MslI MspA1I NcoI NdeI
NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SanDI SapI SexAI SfcI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BfrBI BmtI BsaAI BsaHI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DraIII EciI EcoRV
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI Tth111I
XbaI XcmI XhoI XmaI ZraI