Restriction map for
pF1KE0059
(Linear) (Six-base) MAPSORT of: KIEE0059.seq Check: 57 from: 1 to: 1425
KIEE0059.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 1425
Size: 687 738
AflIII A'CryG_T
Cuts at: 0 633 1425
Size: 633 792
AgeI A'CCGG_T
Cuts at: 0 745 1425
Size: 745 680
AleI CACnn'nnGTG
Cuts at: 0 830 1082 1166 1334 1425
Size: 830 252 84 168 91
Fragments arranged by size:
830 252 168 91 84
ApoI r'AATT_y
Cuts at: 0 569 613 653 821 905 989 1073 1157
Size: 569 44 40 168 84 84 84 84
Cuts at: 1157 1241 1325 1409 1425
Size: 84 84 84 16
Fragments arranged by size:
569 168 84 84 84 84 84 84
84 44 40 16
AseI AT'TA_AT
Cuts at: 0 488 1425
Size: 488 937
BanII G_rGCy'C
Cuts at: 0 191 883 967 1135 1425
Size: 191 692 84 168 290
Fragments arranged by size:
692 290 191 168 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 503 1425
Size: 503 922
BbvCI CC'TCA_GC
Cuts at: 0 5 1425
Size: 5 1420
BglII A'GATC_T
Cuts at: 0 231 1425
Size: 231 1194
BlpI GC'TnA_GC
Cuts at: 0 1050 1425
Size: 1050 375
BmrI ACTGGGnnnn_n'
Cuts at: 0 400 1425
Size: 400 1025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 1425
Size: 327 1098
Bpu10I CC'TnA_GC
Cuts at: 0 5 149 1425
Size: 5 144 1276
Fragments arranged by size:
1276 144 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 355 1425
Size: 140 215 1070
Fragments arranged by size:
1070 215 140
BsaWI w'CCGG_w
Cuts at: 0 745 1425
Size: 745 680
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1425
Size: 67 1358
BsiHKAI G_wGCw'C
Cuts at: 0 883 967 1135 1425
Size: 883 84 168 290
Fragments arranged by size:
883 290 168 84
Bsp1286I G_dGCh'C
Cuts at: 0 191 883 967 1135 1425
Size: 191 692 84 168 290
Fragments arranged by size:
692 290 191 168 84
BsrFI r'CCGG_y
Cuts at: 0 745 1425
Size: 745 680
BstYI r'GATC_y
Cuts at: 0 231 1389 1425
Size: 231 1158 36
Fragments arranged by size:
1158 231 36
ClaI AT'CG_AT
Cuts at: 0 241 1425
Size: 241 1184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1008 1043 1425
Size: 1008 35 382
Fragments arranged by size:
1008 382 35
EcoICRI GAG'CTC
Cuts at: 0 881 965 1133 1425
Size: 881 84 168 292
Fragments arranged by size:
881 292 168 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 687 1425
Size: 327 360 738
Fragments arranged by size:
738 360 327
EcoRI G'AATT_C
Cuts at: 0 569 821 905 989 1073 1157 1241 1325
Size: 569 252 84 84 84 84 84 84
Cuts at: 1325 1409 1425
Size: 84 16
Fragments arranged by size:
569 252 84 84 84 84 84 84
84 16
EcoRV GAT'ATC
Cuts at: 0 397 1425
Size: 397 1028
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 223 255 1425
Size: 223 32 1170
Fragments arranged by size:
1170 223 32
HincII GTy'rAC
Cuts at: 0 1147 1425
Size: 1147 278
HindIII A'AGCT_T
Cuts at: 0 970 1425
Size: 970 455
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 1425
Size: 86 1339
MslI CAynn'nnrTG
Cuts at: 0 578 830 914 998 1082 1166 1250 1266
Size: 578 252 84 84 84 84 84 16
Cuts at: 1266 1334 1425
Size: 68 91
Fragments arranged by size:
578 252 91 84 84 84 84 84
68 16
NdeI CA'TA_TG
Cuts at: 0 1263 1425
Size: 1263 162
NspI r_CATG'y
Cuts at: 0 444 637 901 1425
Size: 444 193 264 524
Fragments arranged by size:
524 444 264 193
PciI A'CATG_T
Cuts at: 0 633 1425
Size: 633 792
SacI G_AGCT'C
Cuts at: 0 883 967 1135 1425
Size: 883 84 168 290
Fragments arranged by size:
883 290 168 84
ScaI AGT'ACT
Cuts at: 0 340 1425
Size: 340 1085
SmlI C'TyrA_G
Cuts at: 0 155 334 1425
Size: 155 179 1091
Fragments arranged by size:
1091 179 155
SphI G_CATG'C
Cuts at: 0 901 1425
Size: 901 524
SspI AAT'ATT
Cuts at: 0 425 1425
Size: 425 1000
StuI AGG'CCT
Cuts at: 0 221 532 700 784 1204 1288 1425
Size: 221 311 168 84 420 84 137
Fragments arranged by size:
420 311 221 168 137 84 84
TatI w'GTAC_w
Cuts at: 0 338 1425
Size: 338 1087
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 1211 1243 1425
Size: 229 32 950 32 182
Fragments arranged by size:
950 229 182 32 32
Enzymes that do cut:
AcuI AflIII AgeI AleI ApoI AseI BanII BbsI
BbvCI BglII BlpI BmrI BpmI Bpu10I BpuEI BsaWI
BseRI BsiHKAI Bsp1286I BsrFI BstYI ClaI CspCI EcoICRI
Eco57MI EcoRI EcoRV Hin4I HincII HindIII MmeI MslI
NdeI NspI PciI SacI ScaI SmlI SphI SspI
StuI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI Bme1580I BmgBI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoNI EcoO109I FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StyI
SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI