Restriction map for
pF1KE0067
(Linear) (Six-base) MAPSORT of: KIEE0067.seq Check: 654 from: 1 to: 4341
KIEE0067.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1362 4341
Size: 1362 2979
AatII G_ACGT'C
Cuts at: 0 2626 4341
Size: 2626 1715
AccI GT'mk_AC
Cuts at: 0 922 2131 4341
Size: 922 1209 2210
Fragments arranged by size:
2210 1209 922
Acc65I G'GTAC_C
Cuts at: 0 27 2039 4341
Size: 27 2012 2302
Fragments arranged by size:
2302 2012 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1438 1495 3099 3359 4229 4341
Size: 1438 57 1604 260 870 112
Fragments arranged by size:
1604 1438 870 260 112 57
AgeI A'CCGG_T
Cuts at: 0 88 4341
Size: 88 4253
AleI CACnn'nnGTG
Cuts at: 0 1699 4341
Size: 1699 2642
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4334 4341
Size: 4334 7
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2399 2431 4064 4096 4341
Size: 2399 32 1633 32 245
Fragments arranged by size:
2399 1633 245 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 442 1063 4207 4341
Size: 442 621 3144 134
Fragments arranged by size:
3144 621 442 134
ApaI G_GGCC'C
Cuts at: 0 4115 4341
Size: 4115 226
ApaLI G'TGCA_C
Cuts at: 0 1235 4341
Size: 1235 3106
ApoI r'AATT_y
Cuts at: 0 623 686 1768 1976 2749 3062 3075 4341
Size: 623 63 1082 208 773 313 13 1266
Fragments arranged by size:
1266 1082 773 623 313 208 63 13
AvaI C'yCGr_G
Cuts at: 0 560 3689 4341
Size: 560 3129 652
Fragments arranged by size:
3129 652 560
BamHI G'GATC_C
Cuts at: 0 3461 4341
Size: 3461 880
BanI G'GyrC_C
Cuts at: 0 27 1346 2039 3756 3975 4341
Size: 27 1319 693 1717 219 366
Fragments arranged by size:
1717 1319 693 366 219 27
BanII G_rGCy'C
Cuts at: 0 500 974 2299 3646 3687 4115 4341
Size: 500 474 1325 1347 41 428 226
Fragments arranged by size:
1347 1325 500 474 428 226 41
BbeI G_GCGC'C
Cuts at: 0 1350 4341
Size: 1350 2991
BbsI GAAGACnn'nnnn_
Cuts at: 0 1777 2699 4341
Size: 1777 922 1642
Fragments arranged by size:
1777 1642 922
BciVI GTATCCnnnnn_n'
Cuts at: 0 1151 4341
Size: 1151 3190
BclI T'GATC_A
Cuts at: 0 212 2178 3299 4341
Size: 212 1966 1121 1042
Fragments arranged by size:
1966 1121 1042 212
BglI GCCn_nnn'nGGC
Cuts at: 0 3691 4341
Size: 3691 650
BglII A'GATC_T
Cuts at: 0 2514 4341
Size: 2514 1827
BlpI GC'TnA_GC
Cuts at: 0 636 4332 4341
Size: 636 3696 9
Fragments arranged by size:
3696 636 9
Bme1580I G_kGCm'C
Cuts at: 0 1079 1239 1872 3777 4115 4341
Size: 1079 160 633 1905 338 226
Fragments arranged by size:
1905 1079 633 338 226 160
BmgBI CAC'GTC
Cuts at: 0 1860 4341
Size: 1860 2481
BmrI ACTGGGnnnn_n'
Cuts at: 0 2461 3601 3714 3771 4341
Size: 2461 1140 113 57 570
Fragments arranged by size:
2461 1140 570 113 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2252 4341
Size: 2252 2089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1257 1383 2041 2077 2129 3679 4341
Size: 602 655 126 658 36 52 1550 662
Fragments arranged by size:
1550 662 658 655 602 126 52 36
Bpu10I CC'TnA_GC
Cuts at: 0 1253 3953 4341
Size: 1253 2700 388
Fragments arranged by size:
2700 1253 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 642 2153 4341
Size: 460 182 1511 2188
Fragments arranged by size:
2188 1511 460 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 290 4341
Size: 290 4051
BsaAI yAC'GTr
Cuts at: 0 2816 4341
Size: 2816 1525
BsaBI GATnn'nnATC
Cuts at: 0 2477 4341
Size: 2477 1864
BsaHI Gr'CG_yC
Cuts at: 0 1347 1820 2623 4341
Size: 1347 473 803 1718
Fragments arranged by size:
1718 1347 803 473
BsaWI w'CCGG_w
Cuts at: 0 88 2648 4341
Size: 88 2560 1693
Fragments arranged by size:
2560 1693 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 152 182 194 224 452 482 1094 1124
Size: 152 30 12 30 228 30 612 30
Cuts at: 1124 2399 2429 2823 2853 4341
Size: 1275 30 394 30 1488
Fragments arranged by size:
1488 1275 612 394 228 152 30 30
30 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 215 1925 2228 3263 3985 4234 4341
Size: 163 52 1710 303 1035 722 249 107
Fragments arranged by size:
1710 1035 722 303 249 163 107 52
BseYI C'CCAG_C
Cuts at: 0 2588 3380 4341
Size: 2588 792 961
Fragments arranged by size:
2588 961 792
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1677 2786 3659 4341
Size: 1677 1109 873 682
Fragments arranged by size:
1677 1109 873 682
BsiEI CG_ry'CG
Cuts at: 0 198 4341
Size: 198 4143
BsiHKAI G_wGCw'C
Cuts at: 0 974 1239 3646 4341
Size: 974 265 2407 695
Fragments arranged by size:
2407 974 695 265
BsmI GAATG_Cn'
Cuts at: 0 233 3957 4341
Size: 233 3724 384
Fragments arranged by size:
3724 384 233
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1342 1827 1890 3803 4341
Size: 1342 485 63 1913 538
Fragments arranged by size:
1913 1342 538 485 63
Bsp1286I G_dGCh'C
Cuts at: 0 500 974 1079 1239 1872 2299 3646 3687
Size: 500 474 105 160 633 427 1347 41
Cuts at: 3687 3777 4115 4341
Size: 90 338 226
Fragments arranged by size:
1347 633 500 474 427 338 226 160
105 90 41
BspHI T'CATG_A
Cuts at: 0 2225 3468 3701 4304 4341
Size: 2225 1243 233 603 37
Fragments arranged by size:
2225 1243 603 233 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1362 1607 3299 4341
Size: 1362 245 1692 1042
Fragments arranged by size:
1692 1362 1042 245
BsrDI GCAATG_nn'
Cuts at: 0 586 4341
Size: 586 3755
BsrFI r'CCGG_y
Cuts at: 0 88 1966 4341
Size: 88 1878 2375
Fragments arranged by size:
2375 1878 88
BsrGI T'GTAC_A
Cuts at: 0 1054 1490 2547 4341
Size: 1054 436 1057 1794
Fragments arranged by size:
1794 1057 1054 436
BssHII G'CGCG_C
Cuts at: 0 71 4341
Size: 71 4270
BssSI C'ACGA_G
Cuts at: 0 2879 4341
Size: 2879 1462
BstAPI GCAn_nnn'nTGC
Cuts at: 0 400 4341
Size: 400 3941
BstBI TT'CG_AA
Cuts at: 0 3060 4341
Size: 3060 1281
BstEII G'GTnAC_C
Cuts at: 0 3309 4341
Size: 3309 1032
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1302 1554 3412 4341
Size: 1302 252 1858 929
Fragments arranged by size:
1858 1302 929 252
BstYI r'GATC_y
Cuts at: 0 696 2514 3030 3461 4171 4341
Size: 696 1818 516 431 710 170
Fragments arranged by size:
1818 710 696 516 431 170
BstZ17I GTA'TAC
Cuts at: 0 923 2132 4341
Size: 923 1209 2209
Fragments arranged by size:
2209 1209 923
BtgI C'CryG_G
Cuts at: 0 185 991 3710 4341
Size: 185 806 2719 631
Fragments arranged by size:
2719 806 631 185
BtsI GCAGTG_nn'
Cuts at: 0 115 3270 4341
Size: 115 3155 1071
Fragments arranged by size:
3155 1071 115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2240 2275 3068 3103 4341
Size: 2240 35 793 35 1238
Fragments arranged by size:
2240 1238 793 35 35
DraI TTT'AAA
Cuts at: 0 1001 4341
Size: 1001 3340
DrdI GACnn_nn'nnGTC
Cuts at: 0 2602 2854 4341
Size: 2602 252 1487
Fragments arranged by size:
2602 1487 252
EaeI y'GGCC_r
Cuts at: 0 1556 3187 4341
Size: 1556 1631 1154
Fragments arranged by size:
1631 1556 1154
EarI CTCTTCn'nnn_
Cuts at: 0 55 2198 3118 3378 4124 4341
Size: 55 2143 920 260 746 217
Fragments arranged by size:
2143 920 746 260 217 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 977 4341
Size: 977 3364
EcoICRI GAG'CTC
Cuts at: 0 972 3644 4341
Size: 972 2672 697
Fragments arranged by size:
2672 972 697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1257 1383 1438 1495 2041 2077 2129
Size: 602 655 126 55 57 546 36 52
Cuts at: 2129 3099 3359 3679 4229 4341
Size: 970 260 320 550 112
Fragments arranged by size:
970 655 602 550 546 320 260 126
112 57 55 52 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1940 4341
Size: 1940 2401
EcoO109I rG'GnC_Cy
Cuts at: 0 1448 2204 3438 4111 4341
Size: 1448 756 1234 673 230
Fragments arranged by size:
1448 1234 756 673 230
EcoRI G'AATT_C
Cuts at: 0 2749 3062 4341
Size: 2749 313 1279
Fragments arranged by size:
2749 1279 313
EcoRV GAT'ATC
Cuts at: 0 3814 4341
Size: 3814 527
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4193 4341
Size: 4193 148
HaeII r_GCGC'y
Cuts at: 0 1350 3749 4341
Size: 1350 2399 592
Fragments arranged by size:
2399 1350 592
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 152 184 358 390 554 586 1094 1126
Size: 152 32 174 32 164 32 508 32
Cuts at: 1126 1289 1321 1726 1758 2686 2718 3706 3738
Size: 163 32 405 32 928 32 988 32
Cuts at: 3738 4341
Size: 603
Fragments arranged by size:
988 928 603 508 405 174 164 163
152 32 32 32 32 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 104 2390 4341
Size: 104 2286 1951
Fragments arranged by size:
2286 1951 104
KasI G'GCGC_C
Cuts at: 0 1346 4341
Size: 1346 2995
KpnI G_GTAC'C
Cuts at: 0 31 2043 4341
Size: 31 2012 2298
Fragments arranged by size:
2298 2012 31
MfeI C'AATT_G
Cuts at: 0 2972 3236 4341
Size: 2972 264 1105
Fragments arranged by size:
2972 1105 264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 367 630 864 1037 2147 2212 3468
Size: 218 149 263 234 173 1110 65 1256
Cuts at: 3468 3749 4341
Size: 281 592
Fragments arranged by size:
1256 1110 592 281 263 234 218 173
149 65
MscI TGG'CCA
Cuts at: 0 1558 3189 4341
Size: 1558 1631 1152
Fragments arranged by size:
1631 1558 1152
MslI CAynn'nnrTG
Cuts at: 0 1552 1699 2224 2260 2527 3173 3263 3328
Size: 1552 147 525 36 267 646 90 65
Cuts at: 3328 3754 4341
Size: 426 587
Fragments arranged by size:
1552 646 587 525 426 267 147 90
65 36
MspA1I CmG'CkG
Cuts at: 0 187 717 1343 2588 4341
Size: 187 530 626 1245 1753
Fragments arranged by size:
1753 1245 626 530 187
NarI GG'CG_CC
Cuts at: 0 1347 4341
Size: 1347 2994
NcoI C'CATG_G
Cuts at: 0 991 3710 4341
Size: 991 2719 631
Fragments arranged by size:
2719 991 631
NspI r_CATG'y
Cuts at: 0 2809 4341
Size: 2809 1532
PflMI CCAn_nnn'nTGG
Cuts at: 0 2789 4341
Size: 2789 1552
PfoI T'CCnGG_A
Cuts at: 0 699 773 4341
Size: 699 74 3568
Fragments arranged by size:
3568 699 74
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2399 2431 4341
Size: 2399 32 1910
Fragments arranged by size:
2399 1910 32
PpuMI rG'GwC_Cy
Cuts at: 0 1448 3438 4341
Size: 1448 1990 903
Fragments arranged by size:
1990 1448 903
PshAI GACnn'nnGTC
Cuts at: 0 3161 4341
Size: 3161 1180
PsiI TTA'TAA
Cuts at: 0 1748 1802 4341
Size: 1748 54 2539
Fragments arranged by size:
2539 1748 54
PspOMI G'GGCC_C
Cuts at: 0 4111 4341
Size: 4111 230
PstI C_TGCA'G
Cuts at: 0 969 1359 2139 3522 4341
Size: 969 390 780 1383 819
Fragments arranged by size:
1383 969 819 780 390
PvuII CAG'CTG
Cuts at: 0 717 1343 2588 4341
Size: 717 626 1245 1753
Fragments arranged by size:
1753 1245 717 626
SacI G_AGCT'C
Cuts at: 0 974 3646 4341
Size: 974 2672 695
Fragments arranged by size:
2672 974 695
SacII CC_GC'GG
Cuts at: 0 188 4341
Size: 188 4153
SanDI GG'GwC_CC
Cuts at: 0 1448 4341
Size: 1448 2893
SapI GCTCTTCn'nnn_
Cuts at: 0 3378 4341
Size: 3378 963
SbfI CC_TGCA'GG
Cuts at: 0 3522 4341
Size: 3522 819
ScaI AGT'ACT
Cuts at: 0 2787 4341
Size: 2787 1554
SexAI A'CCwGG_T
Cuts at: 0 814 2440 4341
Size: 814 1626 1901
Fragments arranged by size:
1901 1626 814
SfcI C'TryA_G
Cuts at: 0 965 1355 2135 3518 4341
Size: 965 390 780 1383 823
Fragments arranged by size:
1383 965 823 780 390
SfoI GGC'GCC
Cuts at: 0 1348 4341
Size: 1348 2993
SmaI CCC'GGG
Cuts at: 0 3691 4341
Size: 3691 650
SmlI C'TyrA_G
Cuts at: 0 475 657 2168 4341
Size: 475 182 1511 2173
Fragments arranged by size:
2173 1511 475 182
SspI AAT'ATT
Cuts at: 0 954 3492 4341
Size: 954 2538 849
Fragments arranged by size:
2538 954 849
StuI AGG'CCT
Cuts at: 0 1760 2726 4341
Size: 1760 966 1615
Fragments arranged by size:
1760 1615 966
StyI C'CwwG_G
Cuts at: 0 245 991 1674 2843 2909 2954 3159 3710
Size: 245 746 683 1169 66 45 205 551
Cuts at: 3710 4149 4341
Size: 439 192
Fragments arranged by size:
1169 746 683 551 439 245 205 192
66 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 212 859 2496 2745 3418 3884 4034 4341
Size: 212 647 1637 249 673 466 150 307
Fragments arranged by size:
1637 673 647 466 307 249 212 150
TatI w'GTAC_w
Cuts at: 0 45 1054 1490 2072 2547 2785 4341
Size: 45 1009 436 582 475 238 1556
Fragments arranged by size:
1556 1009 582 475 436 238 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 107 139 194 226 407 439 452 484
Size: 107 32 55 32 181 32 13 32
Cuts at: 484 1058 1090 2823 2855 2865 2897 4341
Size: 574 32 1733 32 10 32 1444
Fragments arranged by size:
1733 1444 574 181 107 55 32 32
32 32 32 32 32 13 10
XbaI T'CTAG_A
Cuts at: 0 2157 4341
Size: 2157 2184
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 215 512 812 2033 2123 2978 3166 4341
Size: 215 297 300 1221 90 855 188 1175
Fragments arranged by size:
1221 1175 855 300 297 215 188 90
XmaI C'CCGG_G
Cuts at: 0 3689 4341
Size: 3689 652
XmnI GAAnn'nnTTC
Cuts at: 0 1768 2414 4341
Size: 1768 646 1927
Fragments arranged by size:
1927 1768 646
ZraI GAC'GTC
Cuts at: 0 2624 4341
Size: 2624 1717
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtsI CspCI DraI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HindIII KasI KpnI MfeI MmeI
MscI MslI MspA1I NarI NcoI NspI PflMI PfoI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AhdI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI BspEI
BsrBI Bsu36I BtgZI ClaI DraIII EagI FseI FspI
FspAI HincII HpaI MluI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PspXI PsrI PvuI RsrII SalI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI Tth111I XhoI