Restriction map for
pF1KE0069
(Linear) (Six-base) MAPSORT of: KIEE0069.seq Check: 826 from: 1 to: 2214
KIEE0069.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AatII G_ACGT'C
Cuts at: 0 1517 2214
Size: 1517 697
AccI GT'mk_AC
Cuts at: 0 1244 2214
Size: 1244 970
Acc65I G'GTAC_C
Cuts at: 0 310 2214
Size: 310 1904
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1662 2214
Size: 292 1370 552
Fragments arranged by size:
1370 552 292
AfeI AGC'GCT
Cuts at: 0 1420 2214
Size: 1420 794
AleI CACnn'nnGTG
Cuts at: 0 1525 2214
Size: 1525 689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 82 2214
Size: 82 2132
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1567 1599 2214
Size: 1567 32 615
Fragments arranged by size:
1567 615 32
AlwNI CAG_nnn'CTG
Cuts at: 0 741 903 1941 2214
Size: 741 162 1038 273
Fragments arranged by size:
1038 741 273 162
ApaI G_GGCC'C
Cuts at: 0 1821 2214
Size: 1821 393
ApaLI G'TGCA_C
Cuts at: 0 1595 2214
Size: 1595 619
AvaI C'yCGr_G
Cuts at: 0 519 821 1120 1820 2127 2149 2214
Size: 519 302 299 700 307 22 65
Fragments arranged by size:
700 519 307 302 299 65 22
BamHI G'GATC_C
Cuts at: 0 1226 2214
Size: 1226 988
BanI G'GyrC_C
Cuts at: 0 169 310 644 825 1471 1672 1682 2040
Size: 169 141 334 181 646 201 10 358
Cuts at: 2040 2145 2154 2214
Size: 105 9 60
Fragments arranged by size:
646 358 334 201 181 169 141 105
60 10 9
BanII G_rGCy'C
Cuts at: 0 21 349 607 1766 1781 1821 2214
Size: 21 328 258 1159 15 40 393
Fragments arranged by size:
1159 393 328 258 40 21 15
BbeI G_GCGC'C
Cuts at: 0 1676 2149 2158 2214
Size: 1676 473 9 56
Fragments arranged by size:
1676 473 56 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 721 1294 1340 2214
Size: 721 573 46 874
Fragments arranged by size:
874 721 573 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 33 67 2214
Size: 33 34 2147
Fragments arranged by size:
2147 34 33
BglI GCCn_nnn'nGGC
Cuts at: 0 211 395 917 1451 2214
Size: 211 184 522 534 763
Fragments arranged by size:
763 534 522 211 184
BlpI GC'TnA_GC
Cuts at: 0 1235 1775 2214
Size: 1235 540 439
Fragments arranged by size:
1235 540 439
Bme1580I G_kGCm'C
Cuts at: 0 172 428 1001 1476 1559 1599 1810 1821
Size: 172 256 573 475 83 40 211 11
Cuts at: 1821 2214
Size: 393
Fragments arranged by size:
573 475 393 256 211 172 83 40
11
BmgBI CAC'GTC
Cuts at: 0 669 2214
Size: 669 1545
BmrI ACTGGGnnnn_n'
Cuts at: 0 1830 2214
Size: 1830 384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 35 2214
Size: 35 2179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 718 2214
Size: 631 87 1496
Fragments arranged by size:
1496 631 87
Bpu10I CC'TnA_GC
Cuts at: 0 1780 2214
Size: 1780 434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 2214
Size: 1156 1058
BsaI GGTCTCn'nnnn_
Cuts at: 0 526 2214
Size: 526 1688
BsaAI yAC'GTr
Cuts at: 0 1356 2091 2214
Size: 1356 735 123
Fragments arranged by size:
1356 735 123
BsaHI Gr'CG_yC
Cuts at: 0 218 1514 1673 2146 2155 2214
Size: 218 1296 159 473 9 59
Fragments arranged by size:
1296 473 218 159 59 9
BsaWI w'CCGG_w
Cuts at: 0 641 2214
Size: 641 1573
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 1567 1597 2214
Size: 55 30 1482 30 617
Fragments arranged by size:
1482 617 55 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 251 357 1575 1977 2214
Size: 145 106 106 1218 402 237
Fragments arranged by size:
1218 402 237 145 106 106
BseYI C'CCAG_C
Cuts at: 0 1029 1454 2076 2214
Size: 1029 425 622 138
Fragments arranged by size:
1029 622 425 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 2214
Size: 1030 1184
BsiEI CG_ry'CG
Cuts at: 0 453 2214
Size: 453 1761
BsiHKAI G_wGCw'C
Cuts at: 0 53 266 349 1112 1322 1599 1781 2128
Size: 53 213 83 763 210 277 182 347
Cuts at: 2128 2214
Size: 86
Fragments arranged by size:
763 347 277 213 210 182 86 83
53
BsiWI C'GTAC_G
Cuts at: 0 1292 2214
Size: 1292 922
BsmI GAATG_Cn'
Cuts at: 0 1701 2214
Size: 1701 513
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1365 1943 2120 2214
Size: 1365 578 177 94
Fragments arranged by size:
1365 578 177 94
Bsp1286I G_dGCh'C
Cuts at: 0 21 53 172 266 349 428 607 1001
Size: 21 32 119 94 83 79 179 394
Cuts at: 1001 1112 1322 1476 1559 1599 1766 1781 1810
Size: 111 210 154 83 40 167 15 29
Cuts at: 1810 1821 2128 2214
Size: 11 307 86
Fragments arranged by size:
394 307 210 179 167 154 119 111
94 86 83 83 79 40 32 29
21 15 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1052 1103 1230 2018 2214
Size: 1052 51 127 788 196
Fragments arranged by size:
1052 788 196 127 51
BsrBI CCG'CTC
Cuts at: 0 14 449 1224 1653 2214
Size: 14 435 775 429 561
Fragments arranged by size:
775 561 435 429 14
BsrFI r'CCGG_y
Cuts at: 0 276 1079 1160 1432 2214
Size: 276 803 81 272 782
Fragments arranged by size:
803 782 276 272 81
BssSI C'ACGA_G
Cuts at: 0 913 2214
Size: 913 1301
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 1135 2214
Size: 228 907 1079
Fragments arranged by size:
1079 907 228
BstYI r'GATC_y
Cuts at: 0 963 1124 1181 1226 2214
Size: 963 161 57 45 988
Fragments arranged by size:
988 963 161 57 45
BtgI C'CryG_G
Cuts at: 0 326 428 839 1148 1364 1466 1668 2067
Size: 326 102 411 309 216 102 202 399
Cuts at: 2067 2214
Size: 147
Fragments arranged by size:
411 399 326 309 216 202 147 102
102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 183 488 987 2214
Size: 183 305 499 1227
Fragments arranged by size:
1227 499 305 183
BtsI GCAGTG_nn'
Cuts at: 0 183 814 1212 1532 2128 2214
Size: 183 631 398 320 596 86
Fragments arranged by size:
631 596 398 320 183 86
DraIII CAC_nnn'GTG
Cuts at: 0 800 840 1878 2214
Size: 800 40 1038 336
Fragments arranged by size:
1038 800 336 40
DrdI GACnn_nn'nnGTC
Cuts at: 0 187 1513 2214
Size: 187 1326 701
Fragments arranged by size:
1326 701 187
EaeI y'GGCC_r
Cuts at: 0 450 954 1145 1361 1749 2001 2214
Size: 450 504 191 216 388 252 213
Fragments arranged by size:
504 450 388 252 216 213 191
EagI C'GGCC_G
Cuts at: 0 450 2214
Size: 450 1764
EarI CTCTTCn'nnn_
Cuts at: 0 976 2214
Size: 976 1238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 165 667 1147 2214
Size: 165 502 480 1067
Fragments arranged by size:
1067 502 480 165
EcoICRI GAG'CTC
Cuts at: 0 347 1779 2214
Size: 347 1432 435
Fragments arranged by size:
1432 435 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 631 718 1662 2214
Size: 292 339 87 944 552
Fragments arranged by size:
944 552 339 292 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1716 2214
Size: 1716 498
EcoO109I rG'GnC_Cy
Cuts at: 0 635 738 867 1817 1938 2214
Size: 635 103 129 950 121 276
Fragments arranged by size:
950 635 276 129 121 103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2029 2214
Size: 2029 185
FspI TGC'GCA
Cuts at: 0 43 2214
Size: 43 2171
HaeII r_GCGC'y
Cuts at: 0 1422 1676 2101 2149 2158 2214
Size: 1422 254 425 48 9 56
Fragments arranged by size:
1422 425 254 56 48 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 227 259 970 1002 1478 1510 1768 1800
Size: 227 32 711 32 476 32 258 32
Cuts at: 1800 2100 2132 2214
Size: 300 32 82
Fragments arranged by size:
711 476 300 258 227 82 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1245 2214
Size: 1245 969
KasI G'GCGC_C
Cuts at: 0 1672 2145 2154 2214
Size: 1672 473 9 60
Fragments arranged by size:
1672 473 60 9
KpnI G_GTAC'C
Cuts at: 0 314 2214
Size: 314 1900
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2015 2028 2214
Size: 2015 13 186
Fragments arranged by size:
2015 186 13
MscI TGG'CCA
Cuts at: 0 956 2003 2214
Size: 956 1047 211
Fragments arranged by size:
1047 956 211
MslI CAynn'nnrTG
Cuts at: 0 64 1147 1525 2214
Size: 64 1083 378 689
Fragments arranged by size:
1083 689 378 64
MspA1I CmG'CkG
Cuts at: 0 512 632 1167 1550 1670 2069 2137 2169
Size: 512 120 535 383 120 399 68 32
Cuts at: 2169 2214
Size: 45
Fragments arranged by size:
535 512 399 383 120 120 68 45
32
NaeI GCC'GGC
Cuts at: 0 1162 2214
Size: 1162 1052
NarI GG'CG_CC
Cuts at: 0 1673 2146 2155 2214
Size: 1673 473 9 59
Fragments arranged by size:
1673 473 59 9
NgoMIV G'CCGG_C
Cuts at: 0 1160 2214
Size: 1160 1054
NotI GC'GGCC_GC
Cuts at: 0 450 2214
Size: 450 1764
PasI CC'CwG_GG
Cuts at: 0 1766 2214
Size: 1766 448
PfoI T'CCnGG_A
Cuts at: 0 128 2214
Size: 128 2086
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1567 1599 2214
Size: 1567 32 615
Fragments arranged by size:
1567 615 32
PpuMI rG'GwC_Cy
Cuts at: 0 1938 2214
Size: 1938 276
PspOMI G'GGCC_C
Cuts at: 0 1817 2214
Size: 1817 397
PstI C_TGCA'G
Cuts at: 0 568 1452 2214
Size: 568 884 762
Fragments arranged by size:
884 762 568
PvuII CAG'CTG
Cuts at: 0 512 1550 2214
Size: 512 1038 664
Fragments arranged by size:
1038 664 512
SacI G_AGCT'C
Cuts at: 0 349 1781 2214
Size: 349 1432 433
Fragments arranged by size:
1432 433 349
SacII CC_GC'GG
Cuts at: 0 1671 2070 2214
Size: 1671 399 144
Fragments arranged by size:
1671 399 144
SalI G'TCGA_C
Cuts at: 0 1243 2214
Size: 1243 971
SbfI CC_TGCA'GG
Cuts at: 0 1452 2214
Size: 1452 762
SexAI A'CCwGG_T
Cuts at: 0 437 2214
Size: 437 1777
SfcI C'TryA_G
Cuts at: 0 564 627 1448 2214
Size: 564 63 821 766
Fragments arranged by size:
821 766 564 63
SfoI GGC'GCC
Cuts at: 0 1674 2147 2156 2214
Size: 1674 473 9 58
Fragments arranged by size:
1674 473 58 9
SmaI CCC'GGG
Cuts at: 0 521 823 2151 2214
Size: 521 302 1328 63
Fragments arranged by size:
1328 521 302 63
SmlI C'TyrA_G
Cuts at: 0 1171 2214
Size: 1171 1043
StuI AGG'CCT
Cuts at: 0 124 949 2214
Size: 124 825 1265
Fragments arranged by size:
1265 825 124
StyI C'CwwG_G
Cuts at: 0 1397 1502 2214
Size: 1397 105 712
Fragments arranged by size:
1397 712 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1459 2214
Size: 1459 755
TatI w'GTAC_w
Cuts at: 0 477 2214
Size: 477 1737
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 55 87 577 609 2214
Size: 55 32 490 32 1605
Fragments arranged by size:
1605 490 55 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1942 2214
Size: 1942 272
XmaI C'CCGG_G
Cuts at: 0 519 821 2149 2214
Size: 519 302 1328 65
Fragments arranged by size:
1328 519 302 65
XmnI GAAnn'nnTTC
Cuts at: 0 1969 2214
Size: 1969 245
ZraI GAC'GTC
Cuts at: 0 1515 2214
Size: 1515 699
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI
BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI PasI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SacII SalI SbfI SexAI SfcI
SfoI SmaI SmlI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AhdI ApoI AscI
AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI
BglII BmtI BsaBI BspEI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
EcoRI EcoRV FseI FspAI HindIII HpaI MfeI MluI
NcoI NdeI NheI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SanDI SapI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI