Restriction map for
pF1KE0076
(Linear) (Six-base) MAPSORT of: KIEE0076.seq Check: 4307 from: 1 to: 4809
KIEE0076.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2250 2281 4210 4809
Size: 2250 31 1929 599
Fragments arranged by size:
2250 1929 599 31
AatII G_ACGT'C
Cuts at: 0 1028 1844 4809
Size: 1028 816 2965
Fragments arranged by size:
2965 1028 816
AccI GT'mk_AC
Cuts at: 0 2192 4809
Size: 2192 2617
Acc65I G'GTAC_C
Cuts at: 0 3207 4400 4694 4809
Size: 3207 1193 294 115
Fragments arranged by size:
3207 1193 294 115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1582 2055 2789 3096 4809
Size: 1582 473 734 307 1713
Fragments arranged by size:
1713 1582 734 473 307
AgeI A'CCGG_T
Cuts at: 0 297 4809
Size: 297 4512
AleI CACnn'nnGTG
Cuts at: 0 422 4809
Size: 422 4387
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 923 4809
Size: 923 3886
AlwNI CAG_nnn'CTG
Cuts at: 0 21 411 685 825 948 1059 1717 1779
Size: 21 390 274 140 123 111 658 62
Cuts at: 1779 2128 2296 3229 3247 4603 4809
Size: 349 168 933 18 1356 206
Fragments arranged by size:
1356 933 658 390 349 274 206 168
140 123 111 62 21 18
ApaI G_GGCC'C
Cuts at: 0 2143 2331 3082 3132 3803 4741 4763 4809
Size: 2143 188 751 50 671 938 22 46
Fragments arranged by size:
2143 938 751 671 188 50 46 22
ApaLI G'TGCA_C
Cuts at: 0 1936 2459 4809
Size: 1936 523 2350
Fragments arranged by size:
2350 1936 523
AvaI C'yCGr_G
Cuts at: 0 790 805 871 1120 1225 2142 2393 3286
Size: 790 15 66 249 105 917 251 893
Cuts at: 3286 4117 4780 4809
Size: 831 663 29
Fragments arranged by size:
917 893 831 790 663 251 249 105
66 29 15
AvrII C'CTAG_G
Cuts at: 0 610 4809
Size: 610 4199
BanI G'GyrC_C
Cuts at: 0 215 2162 2262 2342 2605 2841 3207 3217
Size: 215 1947 100 80 263 236 366 10
Cuts at: 3217 4069 4400 4694 4809
Size: 852 331 294 115
Fragments arranged by size:
1947 852 366 331 294 263 236 215
115 100 80 10
BanII G_rGCy'C
Cuts at: 0 449 1049 1085 1550 2143 2331 2483 3082
Size: 449 600 36 465 593 188 152 599
Cuts at: 3082 3132 3416 3803 4271 4741 4763 4809
Size: 50 284 387 468 470 22 46
Fragments arranged by size:
600 599 593 470 468 465 449 387
284 188 152 50 46 36 22
BbeI G_GCGC'C
Cuts at: 0 219 4809
Size: 219 4590
BbsI GAAGACnn'nnnn_
Cuts at: 0 1478 1590 4809
Size: 1478 112 3219
Fragments arranged by size:
3219 1478 112
BbvCI CC'TCA_GC
Cuts at: 0 818 2853 4809
Size: 818 2035 1956
Fragments arranged by size:
2035 1956 818
BglI GCCn_nnn'nGGC
Cuts at: 0 1062 1260 1720 1735 1855 3330 3364 4809
Size: 1062 198 460 15 120 1475 34 1445
Fragments arranged by size:
1475 1445 1062 460 198 120 34 15
BlpI GC'TnA_GC
Cuts at: 0 1544 2482 3840 4413 4809
Size: 1544 938 1358 573 396
Fragments arranged by size:
1544 1358 938 573 396
Bme1580I G_kGCm'C
Cuts at: 0 1067 1940 2143 2165 2267 2331 2345 2463
Size: 1067 873 203 22 102 64 14 118
Cuts at: 2463 2608 2634 2846 3082 3132 3220 3803 4390
Size: 145 26 212 236 50 88 583 587
Cuts at: 4390 4506 4741 4763 4809
Size: 116 235 22 46
Fragments arranged by size:
1067 873 587 583 236 235 212 203
145 118 116 102 88 64 50 46
26 22 22 14
BmgBI CAC'GTC
Cuts at: 0 3405 4809
Size: 3405 1404
BmrI ACTGGGnnnn_n'
Cuts at: 0 506 1369 1913 2213 2261 2420 2683 2901
Size: 506 863 544 300 48 159 263 218
Cuts at: 2901 3363 3383 3507 3806 4225 4809
Size: 462 20 124 299 419 584
Fragments arranged by size:
863 584 544 506 462 419 300 299
263 218 159 124 48 20
BmtI G_CTAG'C
Cuts at: 0 4520 4809
Size: 4520 289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 762 3504 3516 4809
Size: 762 2742 12 1293
Fragments arranged by size:
2742 1293 762 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 925 1063 2437 2780 3622 4270 4391
Size: 904 21 138 1374 343 842 648 121
Cuts at: 4391 4809
Size: 418
Fragments arranged by size:
1374 904 842 648 418 343 138 121
21
Bpu10I CC'TnA_GC
Cuts at: 0 599 605 686 818 1504 2853 4330 4809
Size: 599 6 81 132 686 1349 1477 479
Fragments arranged by size:
1477 1349 686 599 479 132 81 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1512 3552 4258 4809
Size: 1512 2040 706 551
Fragments arranged by size:
2040 1512 706 551
BsaI GGTCTCn'nnnn_
Cuts at: 0 1341 1386 2818 3475 4007 4809
Size: 1341 45 1432 657 532 802
Fragments arranged by size:
1432 1341 802 657 532 45
BsaBI GATnn'nnATC
Cuts at: 0 376 4809
Size: 376 4433
BsaHI Gr'CG_yC
Cuts at: 0 216 722 1025 1217 1811 1841 4172 4809
Size: 216 506 303 192 594 30 2331 637
Fragments arranged by size:
2331 637 594 506 303 216 192 30
BsaWI w'CCGG_w
Cuts at: 0 297 4809
Size: 297 4512
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 154 184 4477 4507 4809
Size: 154 30 4293 30 302
Fragments arranged by size:
4293 302 154 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 495 556 1292 2535 3246 3249 3252 3255
Size: 495 61 736 1243 711 3 3 3
Cuts at: 3255 3258 4809
Size: 3 1551
Fragments arranged by size:
1551 1243 736 711 495 61 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 205 329 577 623 767 833 1049 1452
Size: 205 124 248 46 144 66 216 403
Cuts at: 1452 1524 1665 1790 2128 2324 2642 2713 3034
Size: 72 141 125 338 196 318 71 321
Cuts at: 3034 4102 4350 4375 4809
Size: 1068 248 25 434
Fragments arranged by size:
1068 434 403 338 321 318 248 248
216 205 196 144 141 125 124 72
71 66 46 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 385 452 2005 4809
Size: 66 319 67 1553 2804
Fragments arranged by size:
2804 1553 319 67 66
BsiEI CG_ry'CG
Cuts at: 0 302 1644 1706 4809
Size: 302 1342 62 3103
Fragments arranged by size:
3103 1342 302 62
BsiHKAI G_wGCw'C
Cuts at: 0 1105 1897 1940 2463 2483 3796 4116 4809
Size: 1105 792 43 523 20 1313 320 693
Fragments arranged by size:
1313 1105 792 693 523 320 43 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 299 2217 4809
Size: 299 1918 2592
Fragments arranged by size:
2592 1918 299
Bsp1286I G_dGCh'C
Cuts at: 0 449 1049 1067 1085 1105 1550 1897 1940
Size: 449 600 18 18 20 445 347 43
Cuts at: 1940 2143 2165 2267 2331 2345 2463 2483 2608
Size: 203 22 102 64 14 118 20 125
Cuts at: 2608 2634 2846 3082 3132 3220 3416 3796 3803
Size: 26 212 236 50 88 196 380 7
Cuts at: 3803 4116 4271 4390 4506 4741 4763 4809
Size: 313 155 119 116 235 22 46
Fragments arranged by size:
600 449 445 380 347 313 236 235
212 203 196 155 125 119 118 116
102 88 64 50 46 43 26 22
22 20 20 18 18 14 7
BspHI T'CATG_A
Cuts at: 0 380 4809
Size: 380 4429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 359 419 1667 2250 2281 2909 4210 4352
Size: 359 60 1248 583 31 628 1301 142
Cuts at: 4352 4809
Size: 457
Fragments arranged by size:
1301 1248 628 583 457 359 142 60
31
BsrBI CCG'CTC
Cuts at: 0 546 573 870 2867 3855 4748 4809
Size: 546 27 297 1997 988 893 61
Fragments arranged by size:
1997 988 893 546 297 61 27
BsrFI r'CCGG_y
Cuts at: 0 297 1184 2509 3052 3525 4174 4809
Size: 297 887 1325 543 473 649 635
Fragments arranged by size:
1325 887 649 635 543 473 297
BsrGI T'GTAC_A
Cuts at: 0 426 4809
Size: 426 4383
BssHII G'CGCG_C
Cuts at: 0 2749 4809
Size: 2749 2060
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1983 2990 3247 3637 4603 4809
Size: 1983 1007 257 390 966 206
Fragments arranged by size:
1983 1007 966 390 257 206
BstEII G'GTnAC_C
Cuts at: 0 1132 4809
Size: 1132 3677
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1263 1885 2415 2691 4254 4809
Size: 1263 622 530 276 1563 555
Fragments arranged by size:
1563 1263 622 555 530 276
BstYI r'GATC_y
Cuts at: 0 1172 1439 4809
Size: 1172 267 3370
Fragments arranged by size:
3370 1172 267
Bsu36I CC'TnA_GG
Cuts at: 0 539 1127 2430 2466 3771 4809
Size: 539 588 1303 36 1305 1038
Fragments arranged by size:
1305 1303 1038 588 539 36
BtgI C'CryG_G
Cuts at: 0 256 653 665 1256 1733 2117 2337 2436
Size: 256 397 12 591 477 384 220 99
Cuts at: 2436 2858 3696 4160 4584 4763 4809
Size: 422 838 464 424 179 46
Fragments arranged by size:
838 591 477 464 424 422 397 384
256 220 179 99 46 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 785 4809
Size: 785 4024
BtsI GCAGTG_nn'
Cuts at: 0 1065 2702 3015 3973 4089 4809
Size: 1065 1637 313 958 116 720
Fragments arranged by size:
1637 1065 958 720 313 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4149 4184 4809
Size: 4149 35 625
Fragments arranged by size:
4149 625 35
DraIII CAC_nnn'GTG
Cuts at: 0 1609 1921 4585 4809
Size: 1609 312 2664 224
Fragments arranged by size:
2664 1609 312 224
DrdI GACnn_nn'nnGTC
Cuts at: 0 3403 3474 4809
Size: 3403 71 1335
Fragments arranged by size:
3403 1335 71
EaeI y'GGCC_r
Cuts at: 0 518 630 662 1076 1277 1433 1641 1703
Size: 518 112 32 414 201 156 208 62
Cuts at: 1703 1727 1736 1847 2028 2048 2114 2361 2654
Size: 24 9 111 181 20 66 247 293
Cuts at: 2654 3869 4077 4237 4809
Size: 1215 208 160 572
Fragments arranged by size:
1215 572 518 414 293 247 208 208
201 181 160 156 112 111 66 62
32 24 20 9
EagI C'GGCC_G
Cuts at: 0 1641 1703 4809
Size: 1641 62 3106
Fragments arranged by size:
3106 1641 62
EarI CTCTTCn'nnn_
Cuts at: 0 2509 4223 4809
Size: 2509 1714 586
Fragments arranged by size:
2509 1714 586
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1203 1476 1596 1782 2366 2734 3720 3744
Size: 1203 273 120 186 584 368 986 24
Cuts at: 3744 4783 4809
Size: 1039 26
Fragments arranged by size:
1203 1039 986 584 368 273 186 120
26 24
EcoICRI GAG'CTC
Cuts at: 0 2481 4809
Size: 2481 2328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 925 1063 1582 2055 2437 2780 2789
Size: 904 21 138 519 473 382 343 9
Cuts at: 2789 3096 3622 4270 4391 4809
Size: 307 526 648 121 418
Fragments arranged by size:
904 648 526 519 473 418 382 343
307 138 121 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 232 403 632 3724 4183 4809
Size: 232 171 229 3092 459 626
Fragments arranged by size:
3092 626 459 232 229 171
EcoO109I rG'GnC_Cy
Cuts at: 0 18 201 387 408 786 945 1669 1944
Size: 18 183 186 21 378 159 724 275
Cuts at: 1944 2139 2328 2649 3078 3079 3128 3129 3800
Size: 195 189 321 429 1 49 1 671
Cuts at: 3800 3833 3992 4033 4341 4737 4738 4759 4809
Size: 33 159 41 308 396 1 21 50
Fragments arranged by size:
724 671 429 396 378 321 308 275
195 189 186 183 159 159 50 49
41 33 21 21 18 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1164 1875 3123 4767 4809
Size: 1164 711 1248 1644 42
Fragments arranged by size:
1644 1248 1164 711 42
HaeII r_GCGC'y
Cuts at: 0 219 2496 3044 3053 4809
Size: 219 2277 548 9 1756
Fragments arranged by size:
2277 1756 548 219 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 784 816 1087 1119 1164 1196 1915 1947
Size: 784 32 271 32 45 32 719 32
Cuts at: 1947 2530 2562 3343 3375 3568 3600 3619 3651
Size: 583 32 781 32 193 32 19 32
Cuts at: 3651 4809
Size: 1158
Fragments arranged by size:
1158 784 781 719 583 271 193 45
32 32 32 32 32 32 32 32
19
HincII GTy'rAC
Cuts at: 0 3906 4809
Size: 3906 903
KasI G'GCGC_C
Cuts at: 0 215 4809
Size: 215 4594
KpnI G_GTAC'C
Cuts at: 0 3211 4404 4698 4809
Size: 3211 1193 294 111
Fragments arranged by size:
3211 1193 294 111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 474 548 3458 3461 3527 4809
Size: 282 192 74 2910 3 66 1282
Fragments arranged by size:
2910 1282 282 192 74 66 3
MscI TGG'CCA
Cuts at: 0 520 632 1435 1738 1849 2656 4079 4809
Size: 520 112 803 303 111 807 1423 730
Fragments arranged by size:
1423 807 803 730 520 303 112 111
MslI CAynn'nnrTG
Cuts at: 0 422 1423 1738 4809
Size: 422 1001 315 3071
Fragments arranged by size:
3071 1001 422 315
MspA1I CmG'CkG
Cuts at: 0 249 287 369 771 981 1557 1693 1726
Size: 249 38 82 402 210 576 136 33
Cuts at: 1726 1907 2128 2646 2708 3766 3868 4146 4312
Size: 181 221 518 62 1058 102 278 166
Cuts at: 4312 4350 4379 4417 4603 4809
Size: 38 29 38 186 206
Fragments arranged by size:
1058 576 518 402 278 249 221 210
206 186 181 166 136 102 82 62
38 38 38 33 29
NaeI GCC'GGC
Cuts at: 0 1186 3054 4176 4809
Size: 1186 1868 1122 633
Fragments arranged by size:
1868 1186 1122 633
NarI GG'CG_CC
Cuts at: 0 216 4809
Size: 216 4593
NcoI C'CATG_G
Cuts at: 0 1733 2337 2858 4763 4809
Size: 1733 604 521 1905 46
Fragments arranged by size:
1905 1733 604 521 46
NgoMIV G'CCGG_C
Cuts at: 0 1184 3052 4174 4809
Size: 1184 1868 1122 635
Fragments arranged by size:
1868 1184 1122 635
NheI G'CTAG_C
Cuts at: 0 4516 4809
Size: 4516 293
NotI GC'GGCC_GC
Cuts at: 0 1641 4809
Size: 1641 3168
NspI r_CATG'y
Cuts at: 0 3969 4809
Size: 3969 840
PasI CC'CwG_GG
Cuts at: 0 15 1059 4809
Size: 15 1044 3750
Fragments arranged by size:
3750 1044 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 21 948 2281 4585 4809
Size: 21 927 1333 2304 224
Fragments arranged by size:
2304 1333 927 224 21
PfoI T'CCnGG_A
Cuts at: 0 65 645 824 845 2230 2590 3425 3917
Size: 65 580 179 21 1385 360 835 492
Cuts at: 3917 4809
Size: 892
Fragments arranged by size:
1385 892 835 580 492 360 179 65
21
PpuMI rG'GwC_Cy
Cuts at: 0 201 387 408 1669 4033 4341 4809
Size: 201 186 21 1261 2364 308 468
Fragments arranged by size:
2364 1261 468 308 201 186 21
PspOMI G'GGCC_C
Cuts at: 0 2139 2327 3078 3128 3799 4737 4759 4809
Size: 2139 188 751 50 671 938 22 50
Fragments arranged by size:
2139 938 751 671 188 50 50 22
PspXI vC'TCGA_Gb
Cuts at: 0 790 4809
Size: 790 4019
PstI C_TGCA'G
Cuts at: 0 416 2995 3243 4184 4317 4599 4679 4809
Size: 416 2579 248 941 133 282 80 130
Fragments arranged by size:
2579 941 416 282 248 133 130 80
PvuII CAG'CTG
Cuts at: 0 369 771 981 1693 4312 4379 4417 4809
Size: 369 402 210 712 2619 67 38 392
Fragments arranged by size:
2619 712 402 392 369 210 67 38
SacI G_AGCT'C
Cuts at: 0 2483 4809
Size: 2483 2326
SanDI GG'GwC_CC
Cuts at: 0 201 1669 4809
Size: 201 1468 3140
Fragments arranged by size:
3140 1468 201
SapI GCTCTTCn'nnn_
Cuts at: 0 4223 4809
Size: 4223 586
SbfI CC_TGCA'GG
Cuts at: 0 4679 4809
Size: 4679 130
ScaI AGT'ACT
Cuts at: 0 79 1834 4809
Size: 79 1755 2975
Fragments arranged by size:
2975 1755 79
SfcI C'TryA_G
Cuts at: 0 412 487 2448 2991 3239 3948 4180 4313
Size: 412 75 1961 543 248 709 232 133
Cuts at: 4313 4595 4675 4689 4809
Size: 282 80 14 120
Fragments arranged by size:
1961 709 543 412 282 248 232 133
120 80 75 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1735 1855 3364 4809
Size: 1735 120 1509 1445
Fragments arranged by size:
1735 1509 1445 120
SfoI GGC'GCC
Cuts at: 0 217 4809
Size: 217 4592
SgrAI Cr'CCGG_yG
Cuts at: 0 1184 4809
Size: 1184 3625
SmaI CCC'GGG
Cuts at: 0 1122 2144 2395 4809
Size: 1122 1022 251 2414
Fragments arranged by size:
2414 1122 1022 251
SmlI C'TyrA_G
Cuts at: 0 790 1491 3567 4273 4809
Size: 790 701 2076 706 536
Fragments arranged by size:
2076 790 706 701 536
SrfI GCCC'GGGC
Cuts at: 0 2144 2395 4809
Size: 2144 251 2414
Fragments arranged by size:
2414 2144 251
StuI AGG'CCT
Cuts at: 0 151 3103 3347 3367 3565 3730 4809
Size: 151 2952 244 20 198 165 1079
Fragments arranged by size:
2952 1079 244 198 165 151 20
StyI C'CwwG_G
Cuts at: 0 233 610 633 968 1682 1733 2337 2678
Size: 233 377 23 335 714 51 604 341
Cuts at: 2678 2858 3212 4080 4535 4763 4809
Size: 180 354 868 455 228 46
Fragments arranged by size:
868 714 604 455 377 354 341 335
233 228 180 51 46 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 525 1932 1969 2180 2360 2401 2491
Size: 175 350 1407 37 211 180 41 90
Cuts at: 2491 2637 2893 3048 3526 4099 4169 4173 4720
Size: 146 256 155 478 573 70 4 547
Cuts at: 4720 4809
Size: 89
Fragments arranged by size:
1407 573 547 478 350 256 211 180
175 155 146 90 89 70 41 37
4
TatI w'GTAC_w
Cuts at: 0 77 426 491 1832 4809
Size: 77 349 65 1341 2977
Fragments arranged by size:
2977 1341 349 77 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 1905 1937 3648 3680 4354 4386
Size: 154 32 1719 32 1711 32 674 32
Cuts at: 4386 4477 4509 4809
Size: 91 32 300
Fragments arranged by size:
1719 1711 674 300 154 91 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4641 4809
Size: 4641 168
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1263 3423 4720 4809
Size: 1263 2160 1297 89
Fragments arranged by size:
2160 1297 1263 89
XhoI C'TCGA_G
Cuts at: 0 790 4809
Size: 790 4019
XmaI C'CCGG_G
Cuts at: 0 1120 2142 2393 4809
Size: 1120 1022 251 2416
Fragments arranged by size:
2416 1120 1022 251
ZraI GAC'GTC
Cuts at: 0 1026 1842 4809
Size: 1026 816 2967
Fragments arranged by size:
2967 1026 816
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AgeI AleI AlfI
AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NspI PasI PflMI PfoI PpuMI PspOMI PspXI
PstI PvuII SacI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgrAI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AhdI AloI ApoI AscI
AseI AsiSI BaeI BamHI BcgI BciVI BclI BfrBI
BglII BsaAI BsiWI BsmI BspEI BsrDI BssSI BstBI
BstZ17I ClaI DraI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI MfeI MluI NdeI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI
RsrII SacII SalI SexAI SgfI SnaBI SpeI SphI
SspI SwaI XbaI XmnI