Restriction map for
pF1KE0079
(Linear) (Six-base) MAPSORT of: KIEE0079.seq Check: 8514 from: 1 to: 1848
KIEE0079.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 63 1510 1602 1848
Size: 63 1447 92 246
Fragments arranged by size:
1447 246 92 63
AccI GT'mk_AC
Cuts at: 0 1646 1848
Size: 1646 202
Acc65I G'GTAC_C
Cuts at: 0 350 1848
Size: 350 1498
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1002 1460 1848
Size: 1002 458 388
Fragments arranged by size:
1002 458 388
AflIII A'CryG_T
Cuts at: 0 164 931 1848
Size: 164 767 917
Fragments arranged by size:
917 767 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1350 1848
Size: 1350 498
AlwNI CAG_nnn'CTG
Cuts at: 0 512 1080 1848
Size: 512 568 768
Fragments arranged by size:
768 568 512
ApaLI G'TGCA_C
Cuts at: 0 339 1848
Size: 339 1509
AscI GG'CGCG_CC
Cuts at: 0 1726 1848
Size: 1726 122
AvaI C'yCGr_G
Cuts at: 0 1477 1783 1812 1848
Size: 1477 306 29 36
Fragments arranged by size:
1477 306 36 29
BanI G'GyrC_C
Cuts at: 0 20 350 412 1522 1848
Size: 20 330 62 1110 326
Fragments arranged by size:
1110 330 326 62 20
BanII G_rGCy'C
Cuts at: 0 1220 1694 1848
Size: 1220 474 154
Fragments arranged by size:
1220 474 154
BbeI G_GCGC'C
Cuts at: 0 24 416 1848
Size: 24 392 1432
Fragments arranged by size:
1432 392 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 161 883 1016 1848
Size: 161 722 133 832
Fragments arranged by size:
832 722 161 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1803 1837 1848
Size: 1803 34 11
Fragments arranged by size:
1803 34 11
BglI GCCn_nnn'nGGC
Cuts at: 0 295 467 676 1848
Size: 295 172 209 1172
Fragments arranged by size:
1172 295 209 172
BlpI GC'TnA_GC
Cuts at: 0 1509 1848
Size: 1509 339
Bme1580I G_kGCm'C
Cuts at: 0 343 931 1848
Size: 343 588 917
Fragments arranged by size:
917 588 343
BmrI ACTGGGnnnn_n'
Cuts at: 0 1308 1332 1404 1848
Size: 1308 24 72 444
Fragments arranged by size:
1308 444 72 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1827 1848
Size: 1827 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 889 1153 1587 1848
Size: 378 511 264 434 261
Fragments arranged by size:
511 434 378 264 261
BsaI GGTCTCn'nnnn_
Cuts at: 0 1463 1711 1848
Size: 1463 248 137
Fragments arranged by size:
1463 248 137
BsaHI Gr'CG_yC
Cuts at: 0 21 413 1848
Size: 21 392 1435
Fragments arranged by size:
1435 392 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1042 1072 1573 1603 1848
Size: 1042 30 501 30 245
Fragments arranged by size:
1042 501 245 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 83 886 1729 1848
Size: 83 803 843 119
Fragments arranged by size:
843 803 119 83
BseYI C'CCAG_C
Cuts at: 0 346 416 1061 1312 1848
Size: 346 70 645 251 536
Fragments arranged by size:
645 536 346 251 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1194 1245 1320 1729 1843 1848
Size: 1194 51 75 409 114 5
Fragments arranged by size:
1194 409 114 75 51 5
BsiHKAI G_wGCw'C
Cuts at: 0 343 1186 1848
Size: 343 843 662
Fragments arranged by size:
843 662 343
Bsp1286I G_dGCh'C
Cuts at: 0 343 931 1186 1220 1694 1848
Size: 343 588 255 34 474 154
Fragments arranged by size:
588 474 343 255 154 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 63 1510 1602 1848
Size: 63 1447 92 246
Fragments arranged by size:
1447 246 92 63
BsrDI GCAATG_nn'
Cuts at: 0 1287 1848
Size: 1287 561
BsrFI r'CCGG_y
Cuts at: 0 307 1818 1848
Size: 307 1511 30
Fragments arranged by size:
1511 307 30
BssHII G'CGCG_C
Cuts at: 0 9 53 398 1726 1848
Size: 9 44 345 1328 122
Fragments arranged by size:
1328 345 122 44 9
BssSI C'ACGA_G
Cuts at: 0 1378 1848
Size: 1378 470
BstAPI GCAn_nnn'nTGC
Cuts at: 0 426 458 1343 1848
Size: 426 32 885 505
Fragments arranged by size:
885 505 426 32
Bsu36I CC'TnA_GG
Cuts at: 0 789 1848
Size: 789 1059
BtgI C'CryG_G
Cuts at: 0 299 494 1694 1848
Size: 299 195 1200 154
Fragments arranged by size:
1200 299 195 154
DraIII CAC_nnn'GTG
Cuts at: 0 395 449 1848
Size: 395 54 1399
Fragments arranged by size:
1399 395 54
DrdI GACnn_nn'nnGTC
Cuts at: 0 802 1245 1848
Size: 802 443 603
Fragments arranged by size:
802 603 443
EaeI y'GGCC_r
Cuts at: 0 296 762 1284 1548 1848
Size: 296 466 522 264 300
Fragments arranged by size:
522 466 300 296 264
EarI CTCTTCn'nnn_
Cuts at: 0 661 1848
Size: 661 1187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 889 1002 1153 1460 1587 1848
Size: 378 511 113 151 307 127 261
Fragments arranged by size:
511 378 307 261 151 127 113
EcoO109I rG'GnC_Cy
Cuts at: 0 288 1055 1848
Size: 288 767 793
Fragments arranged by size:
793 767 288
HaeII r_GCGC'y
Cuts at: 0 24 333 416 1848
Size: 24 309 83 1432
Fragments arranged by size:
1432 309 83 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 78 110 769 801 1042 1074 1848
Size: 78 32 659 32 241 32 774
Fragments arranged by size:
774 659 241 78 32 32 32
KasI G'GCGC_C
Cuts at: 0 20 412 1848
Size: 20 392 1436
Fragments arranged by size:
1436 392 20
KpnI G_GTAC'C
Cuts at: 0 354 1848
Size: 354 1494
MscI TGG'CCA
Cuts at: 0 1286 1550 1848
Size: 1286 264 298
Fragments arranged by size:
1286 298 264
MslI CAynn'nnrTG
Cuts at: 0 1639 1848
Size: 1639 209
MspA1I CmG'CkG
Cuts at: 0 30 1607 1848
Size: 30 1577 241
Fragments arranged by size:
1577 241 30
NaeI GCC'GGC
Cuts at: 0 309 1848
Size: 309 1539
NarI GG'CG_CC
Cuts at: 0 21 413 1848
Size: 21 392 1435
Fragments arranged by size:
1435 392 21
NcoI C'CATG_G
Cuts at: 0 1694 1848
Size: 1694 154
NgoMIV G'CCGG_C
Cuts at: 0 307 1848
Size: 307 1541
NspI r_CATG'y
Cuts at: 0 168 845 935 1848
Size: 168 677 90 913
Fragments arranged by size:
913 677 168 90
PciI A'CATG_T
Cuts at: 0 164 931 1848
Size: 164 767 917
Fragments arranged by size:
917 767 164
PflMI CCAn_nnn'nTGG
Cuts at: 0 1396 1848
Size: 1396 452
PfoI T'CCnGG_A
Cuts at: 0 1763 1848
Size: 1763 85
PspXI vC'TCGA_Gb
Cuts at: 0 1812 1848
Size: 1812 36
PstI C_TGCA'G
Cuts at: 0 74 1848
Size: 74 1774
SfcI C'TryA_G
Cuts at: 0 70 702 1848
Size: 70 632 1146
Fragments arranged by size:
1146 632 70
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 295 1848
Size: 295 1553
SfoI GGC'GCC
Cuts at: 0 22 414 1848
Size: 22 392 1434
Fragments arranged by size:
1434 392 22
SmlI C'TyrA_G
Cuts at: 0 1812 1848
Size: 1812 36
StuI AGG'CCT
Cuts at: 0 1810 1848
Size: 1810 38
StyI C'CwwG_G
Cuts at: 0 531 1694 1841 1848
Size: 531 1163 147 7
Fragments arranged by size:
1163 531 147 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1012 1848
Size: 1012 836
TatI w'GTAC_w
Cuts at: 0 161 808 1848
Size: 161 647 1040
Fragments arranged by size:
1040 647 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 358 390 433 465 1848
Size: 358 32 43 32 1383
Fragments arranged by size:
1383 358 43 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1417 1480 1848
Size: 1417 63 368
Fragments arranged by size:
1417 368 63
XhoI C'TCGA_G
Cuts at: 0 1812 1848
Size: 1812 36
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AlwNI ApaLI
AscI AvaI BanI BanII BbeI BbsI BcgI BglI
BlpI Bme1580I BmrI BplI BpmI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI
BssHII BssSI BstAPI Bsu36I BtgI DraIII DrdI EaeI
EarI Eco57MI EcoO109I HaeII Hin4I KasI KpnI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PfoI PspXI PstI SfcI SfiI SfoI SmlI
StuI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AloI
ApaI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BfrBI BglII BmgBI BmtI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BstBI BstEII BstXI BstYI BstZ17I
BtgZI BtsI ClaI CspCI DraI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XmaI XmnI ZraI