Restriction map for
pF1KE0081
(Linear) (Six-base) MAPSORT of: KIEE0081.seq Check: 9024 from: 1 to: 4146
KIEE0081.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 859 4146
Size: 859 3287
AccI GT'mk_AC
Cuts at: 0 2375 4146
Size: 2375 1771
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1236 2448 3074 4146
Size: 431 805 1212 626 1072
Fragments arranged by size:
1212 1072 805 626 431
AflIII A'CryG_T
Cuts at: 0 2219 4146
Size: 2219 1927
AgeI A'CCGG_T
Cuts at: 0 793 4146
Size: 793 3353
AhdI GACnn_n'nnGTC
Cuts at: 0 1980 3642 4146
Size: 1980 1662 504
Fragments arranged by size:
1980 1662 504
AleI CACnn'nnGTG
Cuts at: 0 4012 4146
Size: 4012 134
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 198 230 4146
Size: 198 32 3916
Fragments arranged by size:
3916 198 32
AlwNI CAG_nnn'CTG
Cuts at: 0 255 1152 2274 2471 2823 4146
Size: 255 897 1122 197 352 1323
Fragments arranged by size:
1323 1122 897 352 255 197
ApaI G_GGCC'C
Cuts at: 0 1573 4146
Size: 1573 2573
ApoI r'AATT_y
Cuts at: 0 1326 2089 4063 4146
Size: 1326 763 1974 83
Fragments arranged by size:
1974 1326 763 83
AvaI C'yCGr_G
Cuts at: 0 535 1462 1540 1690 2502 4146
Size: 535 927 78 150 812 1644
Fragments arranged by size:
1644 927 812 535 150 78
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3322 3355 4146
Size: 3322 33 791
Fragments arranged by size:
3322 791 33
BanI G'GyrC_C
Cuts at: 0 24 4146
Size: 24 4122
BanII G_rGCy'C
Cuts at: 0 1378 1573 1699 2503 2603 3031 3535 3581
Size: 1378 195 126 804 100 428 504 46
Cuts at: 3581 4146
Size: 565
Fragments arranged by size:
1378 804 565 504 428 195 126 100
46
BbsI GAAGACnn'nnnn_
Cuts at: 0 1103 2041 2056 2442 3062 4146
Size: 1103 938 15 386 620 1084
Fragments arranged by size:
1103 1084 938 620 386 15
BbvCI CC'TCA_GC
Cuts at: 0 466 4146
Size: 466 3680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3969 4003 4146
Size: 3969 34 143
Fragments arranged by size:
3969 143 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 892 3323 4146
Size: 892 2431 823
Fragments arranged by size:
2431 892 823
BclI T'GATC_A
Cuts at: 0 845 4146
Size: 845 3301
BglI GCCn_nnn'nGGC
Cuts at: 0 4120 4146
Size: 4120 26
BglII A'GATC_T
Cuts at: 0 1958 3737 4146
Size: 1958 1779 409
Fragments arranged by size:
1958 1779 409
BlpI GC'TnA_GC
Cuts at: 0 980 2479 4146
Size: 980 1499 1667
Fragments arranged by size:
1667 1499 980
Bme1580I G_kGCm'C
Cuts at: 0 29 1482 1573 4146
Size: 29 1453 91 2573
Fragments arranged by size:
2573 1453 91 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 30 4146
Size: 30 4116
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 628 1555 2517 4146
Size: 628 927 962 1629
Fragments arranged by size:
1629 962 927 628
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 147 648 1351 1504 1510 1693 1795
Size: 140 7 501 703 153 6 183 102
Cuts at: 1795 2420 2989 3169 3652 4146
Size: 625 569 180 483 494
Fragments arranged by size:
703 625 569 501 494 483 183 180
153 140 102 7 6
Bpu10I CC'TnA_GC
Cuts at: 0 466 3025 4146
Size: 466 2559 1121
Fragments arranged by size:
2559 1121 466
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 408 2382 3650 4146
Size: 222 186 1974 1268 496
Fragments arranged by size:
1974 1268 496 222 186
BsaI GGTCTCn'nnnn_
Cuts at: 0 1324 2418 2990 4146
Size: 1324 1094 572 1156
Fragments arranged by size:
1324 1156 1094 572
BsaAI yAC'GTr
Cuts at: 0 2001 4146
Size: 2001 2145
BsaHI Gr'CG_yC
Cuts at: 0 1994 3262 4146
Size: 1994 1268 884
Fragments arranged by size:
1994 1268 884
BsaWI w'CCGG_w
Cuts at: 0 793 4146
Size: 793 3353
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 336 366 1813 1843 4146
Size: 336 30 1447 30 2303
Fragments arranged by size:
2303 1447 336 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 651 1444 1690 2035 2353 2356 2605 2628
Size: 651 793 246 345 318 3 249 23
Cuts at: 2628 4146
Size: 1518
Fragments arranged by size:
1518 793 651 345 318 249 246 23
3
BseYI C'CCAG_C
Cuts at: 0 248 1408 1733 4146
Size: 248 1160 325 2413
Fragments arranged by size:
2413 1160 325 248
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1729 2668 3963 4146
Size: 1729 939 1295 183
Fragments arranged by size:
1729 1295 939 183
BsiEI CG_ry'CG
Cuts at: 0 1953 2057 4075 4146
Size: 1953 104 2018 71
Fragments arranged by size:
2018 1953 104 71
BsiHKAI G_wGCw'C
Cuts at: 0 1820 3031 3989 4146
Size: 1820 1211 958 157
Fragments arranged by size:
1820 1211 958 157
Bsp1286I G_dGCh'C
Cuts at: 0 29 1378 1482 1573 1699 1820 2503 2603
Size: 29 1349 104 91 126 121 683 100
Cuts at: 2603 3031 3535 3581 3989 4146
Size: 428 504 46 408 157
Fragments arranged by size:
1349 683 504 428 408 157 126 121
104 100 91 46 29
BspHI T'CATG_A
Cuts at: 0 308 956 3155 4146
Size: 308 648 2199 991
Fragments arranged by size:
2199 991 648 308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 859 1973 3113 4146
Size: 859 1114 1140 1033
Fragments arranged by size:
1140 1114 1033 859
BsrBI CCG'CTC
Cuts at: 0 306 1887 4146
Size: 306 1581 2259
Fragments arranged by size:
2259 1581 306
BsrDI GCAATG_nn'
Cuts at: 0 521 2535 2555 3860 3992 4053 4146
Size: 521 2014 20 1305 132 61 93
Fragments arranged by size:
2014 1305 521 132 93 61 20
BsrFI r'CCGG_y
Cuts at: 0 793 2750 2981 4146
Size: 793 1957 231 1165
Fragments arranged by size:
1957 1165 793 231
BsrGI T'GTAC_A
Cuts at: 0 1061 4146
Size: 1061 3085
BssSI C'ACGA_G
Cuts at: 0 46 2661 4146
Size: 46 2615 1485
Fragments arranged by size:
2615 1485 46
BstAPI GCAn_nnn'nTGC
Cuts at: 0 913 4146
Size: 913 3233
BstEII G'GTnAC_C
Cuts at: 0 2064 4146
Size: 2064 2082
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2286 2398 2907 3043 3927 4146
Size: 2286 112 509 136 884 219
Fragments arranged by size:
2286 884 509 219 136 112
BstYI r'GATC_y
Cuts at: 0 560 1958 3669 3708 3737 4146
Size: 560 1398 1711 39 29 409
Fragments arranged by size:
1711 1398 560 409 39 29
Bsu36I CC'TnA_GG
Cuts at: 0 149 768 2640 4146
Size: 149 619 1872 1506
Fragments arranged by size:
1872 1506 619 149
BtgI C'CryG_G
Cuts at: 0 178 600 2537 2675 3495 3581 4146
Size: 178 422 1937 138 820 86 565
Fragments arranged by size:
1937 820 565 422 178 138 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 86 842 4146
Size: 86 756 3304
Fragments arranged by size:
3304 756 86
BtsI GCAGTG_nn'
Cuts at: 0 902 1346 1821 3278 4146
Size: 902 444 475 1457 868
Fragments arranged by size:
1457 902 868 475 444
ClaI AT'CG_AT
Cuts at: 0 3902 4146
Size: 3902 244
DraI TTT'AAA
Cuts at: 0 3228 4146
Size: 3228 918
DraIII CAC_nnn'GTG
Cuts at: 0 3507 4146
Size: 3507 639
DrdI GACnn_nn'nnGTC
Cuts at: 0 385 4146
Size: 385 3761
EaeI y'GGCC_r
Cuts at: 0 1187 2097 2276 3403 3498 4072 4121 4146
Size: 1187 910 179 1127 95 574 49 25
Fragments arranged by size:
1187 1127 910 574 179 95 49 25
EagI C'GGCC_G
Cuts at: 0 4072 4146
Size: 4072 74
EarI CTCTTCn'nnn_
Cuts at: 0 4 39 572 2285 2701 3568 4146
Size: 4 35 533 1713 416 867 578
Fragments arranged by size:
1713 867 578 533 416 35 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3556 4146
Size: 3556 590
EcoICRI GAG'CTC
Cuts at: 0 3029 4146
Size: 3029 1117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 147 431 648 1236 1351 1504 1510
Size: 140 7 284 217 588 115 153 6
Cuts at: 1510 1693 1795 2420 2448 2989 3074 3169 3652
Size: 183 102 625 28 541 85 95 483
Cuts at: 3652 4146
Size: 494
Fragments arranged by size:
625 588 541 494 483 284 217 183
153 140 115 102 95 85 28 7
6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3753 3769 4146
Size: 3753 16 377
Fragments arranged by size:
3753 377 16
EcoO109I rG'GnC_Cy
Cuts at: 0 1842 1890 4146
Size: 1842 48 2256
Fragments arranged by size:
2256 1842 48
EcoRI G'AATT_C
Cuts at: 0 1326 4146
Size: 1326 2820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3564 4146
Size: 3564 582
FspI TGC'GCA
Cuts at: 0 855 3092 4146
Size: 855 2237 1054
Fragments arranged by size:
2237 1054 855
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 764 796 1966 1998 2888 2920 3628 3660
Size: 764 32 1170 32 890 32 708 32
Cuts at: 3660 4146
Size: 486
Fragments arranged by size:
1170 890 764 708 486 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3243 4146
Size: 3243 903
HindIII A'AGCT_T
Cuts at: 0 318 443 4146
Size: 318 125 3703
Fragments arranged by size:
3703 318 125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 1973 2093 2347 2471 4146
Size: 649 1324 120 254 124 1675
Fragments arranged by size:
1675 1324 649 254 124 120
MscI TGG'CCA
Cuts at: 0 1189 2099 2278 3405 4123 4146
Size: 1189 910 179 1127 718 23
Fragments arranged by size:
1189 1127 910 718 179 23
MslI CAynn'nnrTG
Cuts at: 0 1018 1777 2623 2905 2953 3041 3346 3806
Size: 1018 759 846 282 48 88 305 460
Cuts at: 3806 3925 4012 4146
Size: 119 87 134
Fragments arranged by size:
1018 846 759 460 305 282 134 119
88 87 48
MspA1I CmG'CkG
Cuts at: 0 169 709 1195 1754 2076 2398 2589 2714
Size: 169 540 486 559 322 322 191 125
Cuts at: 2714 2787 3258 3497 3852 4146
Size: 73 471 239 355 294
Fragments arranged by size:
559 540 486 471 355 322 322 294
239 191 169 125 73
NaeI GCC'GGC
Cuts at: 0 2752 4146
Size: 2752 1394
NcoI C'CATG_G
Cuts at: 0 600 2537 3581 4146
Size: 600 1937 1044 565
Fragments arranged by size:
1937 1044 600 565
NdeI CA'TA_TG
Cuts at: 0 1791 4146
Size: 1791 2355
NgoMIV G'CCGG_C
Cuts at: 0 2750 4146
Size: 2750 1396
NspI r_CATG'y
Cuts at: 0 1260 1507 2223 4146
Size: 1260 247 716 1923
Fragments arranged by size:
1923 1260 716 247
PasI CC'CwG_GG
Cuts at: 0 3396 4146
Size: 3396 750
PciI A'CATG_T
Cuts at: 0 2219 4146
Size: 2219 1927
PflMI CCAn_nnn'nTGG
Cuts at: 0 255 606 3131 3412 3507 4035 4146
Size: 255 351 2525 281 95 528 111
Fragments arranged by size:
2525 528 351 281 255 111 95
PfoI T'CCnGG_A
Cuts at: 0 563 1367 1670 4146
Size: 563 804 303 2476
Fragments arranged by size:
2476 804 563 303
PmlI CAC'GTG
Cuts at: 0 2001 4146
Size: 2001 2145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3295 3327 4146
Size: 3295 32 819
Fragments arranged by size:
3295 819 32
PpuMI rG'GwC_Cy
Cuts at: 0 1842 4146
Size: 1842 2304
PshAI GACnn'nnGTC
Cuts at: 0 2056 4146
Size: 2056 2090
PsiI TTA'TAA
Cuts at: 0 98 4146
Size: 98 4048
PspOMI G'GGCC_C
Cuts at: 0 1569 4146
Size: 1569 2577
PspXI vC'TCGA_Gb
Cuts at: 0 1690 4146
Size: 1690 2456
PstI C_TGCA'G
Cuts at: 0 874 1341 2081 3257 3338 4146
Size: 874 467 740 1176 81 808
Fragments arranged by size:
1176 874 808 740 467 81
PvuII CAG'CTG
Cuts at: 0 169 709 2076 2398 2589 2787 3258 3852
Size: 169 540 1367 322 191 198 471 594
Cuts at: 3852 4146
Size: 294
Fragments arranged by size:
1367 594 540 471 322 294 198 191
169
SacI G_AGCT'C
Cuts at: 0 3031 4146
Size: 3031 1115
SacII CC_GC'GG
Cuts at: 0 3498 4146
Size: 3498 648
SapI GCTCTTCn'nnn_
Cuts at: 0 572 2701 4146
Size: 572 2129 1445
Fragments arranged by size:
2129 1445 572
ScaI AGT'ACT
Cuts at: 0 842 2083 4146
Size: 842 1241 2063
Fragments arranged by size:
2063 1241 842
SexAI A'CCwGG_T
Cuts at: 0 1592 4146
Size: 1592 2554
SfcI C'TryA_G
Cuts at: 0 870 1337 2077 2976 3253 3320 3334 4146
Size: 870 467 740 899 277 67 14 812
Fragments arranged by size:
899 870 812 740 467 277 67 14
SgrAI Cr'CCGG_yG
Cuts at: 0 793 4146
Size: 793 3353
SmaI CCC'GGG
Cuts at: 0 1464 4146
Size: 1464 2682
SmlI C'TyrA_G
Cuts at: 0 201 423 1690 2361 3665 4146
Size: 201 222 1267 671 1304 481
Fragments arranged by size:
1304 1267 671 481 222 201
SphI G_CATG'C
Cuts at: 0 1260 1507 4146
Size: 1260 247 2639
Fragments arranged by size:
2639 1260 247
SspI AAT'ATT
Cuts at: 0 545 571 4146
Size: 545 26 3575
Fragments arranged by size:
3575 545 26
StuI AGG'CCT
Cuts at: 0 1527 1741 3023 4146
Size: 1527 214 1282 1123
Fragments arranged by size:
1527 1282 1123 214
StyI C'CwwG_G
Cuts at: 0 600 1604 2537 2654 3173 3548 3581 3749
Size: 600 1004 933 117 519 375 33 168
Cuts at: 3749 3770 4146
Size: 21 376
Fragments arranged by size:
1004 933 600 519 376 375 168 117
33 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 12 3477 4146
Size: 12 3465 669
Fragments arranged by size:
3465 669 12
TatI w'GTAC_w
Cuts at: 0 531 840 1061 2081 3338 4146
Size: 531 309 221 1020 1257 808
Fragments arranged by size:
1257 1020 808 531 309 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 336 347 368 379 1813 1845 2115 2147
Size: 336 11 21 11 1434 32 270 32
Cuts at: 2147 2297 2329 3375 3407 4146
Size: 150 32 1046 32 739
Fragments arranged by size:
1434 1046 739 336 270 150 32 32
32 32 21 11 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1416 2544 3132 4146
Size: 1416 1128 588 1014
Fragments arranged by size:
1416 1128 1014 588
XhoI C'TCGA_G
Cuts at: 0 1690 4146
Size: 1690 2456
XmaI C'CCGG_G
Cuts at: 0 1462 4146
Size: 1462 2684
XmnI GAAnn'nnTTC
Cuts at: 0 1360 2764 4146
Size: 1360 1404 1382
Fragments arranged by size:
1404 1382 1360
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AhdI AleI AloI
AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I HincII
HindIII MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuII SacI
SacII SapI ScaI SexAI SfcI SgrAI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AlfI ApaLI AscI
AseI AsiSI AvrII BamHI BbeI BfrBI BmgBI BmtI
BsaBI BsiWI BsmI BsmBI BspEI BssHII BstBI BstZ17I
CspCI EcoRV FseI FspAI HaeII HpaI KasI KpnI
MfeI MluI NarI NheI NotI NruI NsiI PacI
PmeI PsrI PvuI RsrII SalI SanDI SbfI SfiI
SfoI SgfI SnaBI SpeI SrfI SwaI Tth111I XbaI
ZraI