Restriction map for
pF1KE0084
(Linear) (Six-base) MAPSORT of: KIEE0084.seq Check: 9320 from: 1 to: 2616
KIEE0084.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1941 2616
Size: 1941 675
AatII G_ACGT'C
Cuts at: 0 857 2616
Size: 857 1759
AccI GT'mk_AC
Cuts at: 0 836 2616
Size: 836 1780
Acc65I G'GTAC_C
Cuts at: 0 2492 2616
Size: 2492 124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1215 1481 1564 1675 2021 2403 2616
Size: 592 623 266 83 111 346 382 213
Fragments arranged by size:
623 592 382 346 266 213 111 83
AflIII A'CryG_T
Cuts at: 0 377 2616
Size: 377 2239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1667 2616
Size: 1667 949
AlwNI CAG_nnn'CTG
Cuts at: 0 702 1126 2616
Size: 702 424 1490
Fragments arranged by size:
1490 702 424
ApaI G_GGCC'C
Cuts at: 0 2305 2616
Size: 2305 311
ApoI r'AATT_y
Cuts at: 0 1568 1597 2616
Size: 1568 29 1019
Fragments arranged by size:
1568 1019 29
AvaI C'yCGr_G
Cuts at: 0 169 453 2162 2267 2616
Size: 169 284 1709 105 349
Fragments arranged by size:
1709 349 284 169 105
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 786 819 2616
Size: 786 33 1797
Fragments arranged by size:
1797 786 33
BanI G'GyrC_C
Cuts at: 0 135 733 1984 2492 2616
Size: 135 598 1251 508 124
Fragments arranged by size:
1251 598 508 135 124
BanII G_rGCy'C
Cuts at: 0 8 260 754 1133 2305 2616
Size: 8 252 494 379 1172 311
Fragments arranged by size:
1172 494 379 311 252 8
BbeI G_GCGC'C
Cuts at: 0 139 2616
Size: 139 2477
BbsI GAAGACnn'nnnn_
Cuts at: 0 1499 1532 1847 2616
Size: 1499 33 315 769
Fragments arranged by size:
1499 769 315 33
BbvCI CC'TCA_GC
Cuts at: 0 2208 2616
Size: 2208 408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2258 2292 2616
Size: 2258 34 324
Fragments arranged by size:
2258 324 34
BclI T'GATC_A
Cuts at: 0 932 2616
Size: 932 1684
BlpI GC'TnA_GC
Cuts at: 0 277 753 1127 2616
Size: 277 476 374 1489
Fragments arranged by size:
1489 476 374 277
Bme1580I G_kGCm'C
Cuts at: 0 768 1987 2305 2616
Size: 768 1219 318 311
Fragments arranged by size:
1219 768 318 311
BmgBI CAC'GTC
Cuts at: 0 147 2616
Size: 147 2469
BmrI ACTGGGnnnn_n'
Cuts at: 0 280 2168 2616
Size: 280 1888 448
Fragments arranged by size:
1888 448 280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 468 2616
Size: 468 2148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 364 496 1167 1267 2419 2616
Size: 226 138 132 671 100 1152 197
Fragments arranged by size:
1152 671 226 197 138 132 100
Bpu10I CC'TnA_GC
Cuts at: 0 2208 2616
Size: 2208 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 2616
Size: 1054 1562
BsaI GGTCTCn'nnnn_
Cuts at: 0 342 895 1279 2411 2616
Size: 342 553 384 1132 205
Fragments arranged by size:
1132 553 384 342 205
BsaHI Gr'CG_yC
Cuts at: 0 136 338 854 1019 2616
Size: 136 202 516 165 1597
Fragments arranged by size:
1597 516 202 165 136
BsaWI w'CCGG_w
Cuts at: 0 722 2616
Size: 722 1894
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2305 2335 2476 2506 2616
Size: 2305 30 141 30 110
Fragments arranged by size:
2305 141 110 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 895 907 1980 2189 2407 2616
Size: 130 765 12 1073 209 218 209
Fragments arranged by size:
1073 765 218 209 209 130 12
BseYI C'CCAG_C
Cuts at: 0 81 359 1269 1784 2539 2616
Size: 81 278 910 515 755 77
Fragments arranged by size:
910 755 515 278 81 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1096 1975 2616
Size: 1096 879 641
Fragments arranged by size:
1096 879 641
BsiEI CG_ry'CG
Cuts at: 0 107 219 2616
Size: 107 112 2397
Fragments arranged by size:
2397 112 107
BsiHKAI G_wGCw'C
Cuts at: 0 1133 2616
Size: 1133 1483
BsmI GAATG_Cn'
Cuts at: 0 1768 2616
Size: 1768 848
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1026 1221 1588 2328 2503 2616
Size: 1026 195 367 740 175 113
Fragments arranged by size:
1026 740 367 195 175 113
Bsp1286I G_dGCh'C
Cuts at: 0 8 260 754 768 1133 1987 2305 2616
Size: 8 252 494 14 365 854 318 311
Fragments arranged by size:
854 494 365 318 311 252 14 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1941 1947 2165 2303 2616
Size: 1941 6 218 138 313
Fragments arranged by size:
1941 313 218 138 6
BsrBI CCG'CTC
Cuts at: 0 120 2616
Size: 120 2496
BsrDI GCAATG_nn'
Cuts at: 0 663 2616
Size: 663 1953
BsrFI r'CCGG_y
Cuts at: 0 106 311 2616
Size: 106 205 2305
Fragments arranged by size:
2305 205 106
BssHII G'CGCG_C
Cuts at: 0 13 2616
Size: 13 2603
BssSI C'ACGA_G
Cuts at: 0 1304 1915 2389 2616
Size: 1304 611 474 227
Fragments arranged by size:
1304 611 474 227
BstAPI GCAn_nnn'nTGC
Cuts at: 0 702 2616
Size: 702 1914
BstEII G'GTnAC_C
Cuts at: 0 1477 2103 2616
Size: 1477 626 513
Fragments arranged by size:
1477 626 513
BstYI r'GATC_y
Cuts at: 0 521 698 2616
Size: 521 177 1918
Fragments arranged by size:
1918 521 177
BtgI C'CryG_G
Cuts at: 0 352 1484 2234 2616
Size: 352 1132 750 382
Fragments arranged by size:
1132 750 382 352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2204 2239 2616
Size: 2204 35 377
Fragments arranged by size:
2204 377 35
DraIII CAC_nnn'GTG
Cuts at: 0 2103 2616
Size: 2103 513
EaeI y'GGCC_r
Cuts at: 0 104 216 263 665 941 2616
Size: 104 112 47 402 276 1675
Fragments arranged by size:
1675 402 276 112 104 47
EagI C'GGCC_G
Cuts at: 0 104 216 2616
Size: 104 112 2400
Fragments arranged by size:
2400 112 104
EarI CTCTTCn'nnn_
Cuts at: 0 2261 2616
Size: 2261 355
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 1504 2616
Size: 74 1430 1112
Fragments arranged by size:
1430 1112 74
EcoICRI GAG'CTC
Cuts at: 0 1131 2616
Size: 1131 1485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 364 496 592 1167 1215 1267 1481
Size: 226 138 132 96 575 48 52 214
Cuts at: 1481 1564 1675 2021 2403 2419 2616
Size: 83 111 346 382 16 197
Fragments arranged by size:
575 382 346 226 214 197 138 132
111 96 83 52 48 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2500 2616
Size: 2500 116
EcoO109I rG'GnC_Cy
Cuts at: 0 194 239 927 2071 2170 2301 2302 2616
Size: 194 45 688 1144 99 131 1 314
Fragments arranged by size:
1144 688 314 194 131 99 45 1
EcoRI G'AATT_C
Cuts at: 0 1568 2616
Size: 1568 1048
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1926 2616
Size: 1926 690
FspI TGC'GCA
Cuts at: 0 706 2616
Size: 706 1910
HaeII r_GCGC'y
Cuts at: 0 139 169 367 2616
Size: 139 30 198 2249
Fragments arranged by size:
2249 198 139 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1240 1272 2305 2327 2337 2359 2616
Size: 1240 32 1033 22 10 22 257
Fragments arranged by size:
1240 1033 257 32 22 22 10
HincII GTy'rAC
Cuts at: 0 2385 2616
Size: 2385 231
HindIII A'AGCT_T
Cuts at: 0 742 2616
Size: 742 1874
KasI G'GCGC_C
Cuts at: 0 135 2616
Size: 135 2481
KpnI G_GTAC'C
Cuts at: 0 2496 2616
Size: 2496 120
MluI A'CGCG_T
Cuts at: 0 377 2616
Size: 377 2239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 2616
Size: 581 2035
MscI TGG'CCA
Cuts at: 0 265 667 943 2616
Size: 265 402 276 1673
Fragments arranged by size:
1673 402 276 265
MslI CAynn'nnrTG
Cuts at: 0 610 2616
Size: 610 2006
MspA1I CmG'CkG
Cuts at: 0 234 276 354 1042 1123 1245 1999 2543
Size: 234 42 78 688 81 122 754 544
Cuts at: 2543 2616
Size: 73
Fragments arranged by size:
754 688 544 234 122 81 78 73
42
NaeI GCC'GGC
Cuts at: 0 108 2616
Size: 108 2508
NarI GG'CG_CC
Cuts at: 0 136 2616
Size: 136 2480
NcoI C'CATG_G
Cuts at: 0 1484 2616
Size: 1484 1132
NdeI CA'TA_TG
Cuts at: 0 1809 2616
Size: 1809 807
NgoMIV G'CCGG_C
Cuts at: 0 106 2616
Size: 106 2510
NotI GC'GGCC_GC
Cuts at: 0 216 2616
Size: 216 2400
NspI r_CATG'y
Cuts at: 0 600 711 1671 1932 2616
Size: 600 111 960 261 684
Fragments arranged by size:
960 684 600 261 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 977 1269 2616
Size: 381 596 292 1347
Fragments arranged by size:
1347 596 381 292
PfoI T'CCnGG_A
Cuts at: 0 1675 2616
Size: 1675 941
PpuMI rG'GwC_Cy
Cuts at: 0 194 239 2170 2616
Size: 194 45 1931 446
Fragments arranged by size:
1931 446 194 45
PspOMI G'GGCC_C
Cuts at: 0 2301 2616
Size: 2301 315
PstI C_TGCA'G
Cuts at: 0 698 2300 2616
Size: 698 1602 316
Fragments arranged by size:
1602 698 316
PvuII CAG'CTG
Cuts at: 0 1123 1245 1999 2616
Size: 1123 122 754 617
Fragments arranged by size:
1123 754 617 122
RsrII CG'GwC_CG
Cuts at: 0 2313 2616
Size: 2313 303
SacI G_AGCT'C
Cuts at: 0 1133 2616
Size: 1133 1483
SacII CC_GC'GG
Cuts at: 0 355 2616
Size: 355 2261
SanDI GG'GwC_CC
Cuts at: 0 194 2170 2616
Size: 194 1976 446
Fragments arranged by size:
1976 446 194
SbfI CC_TGCA'GG
Cuts at: 0 2300 2616
Size: 2300 316
SexAI A'CCwGG_T
Cuts at: 0 839 1181 2099 2616
Size: 839 342 918 517
Fragments arranged by size:
918 839 517 342
SfcI C'TryA_G
Cuts at: 0 694 2088 2296 2616
Size: 694 1394 208 320
Fragments arranged by size:
1394 694 320 208
SfoI GGC'GCC
Cuts at: 0 137 2616
Size: 137 2479
SmlI C'TyrA_G
Cuts at: 0 7 1069 2616
Size: 7 1062 1547
Fragments arranged by size:
1547 1062 7
SphI G_CATG'C
Cuts at: 0 711 2616
Size: 711 1905
StuI AGG'CCT
Cuts at: 0 1617 2616
Size: 1617 999
StyI C'CwwG_G
Cuts at: 0 161 651 1484 1979 2616
Size: 161 490 833 495 637
Fragments arranged by size:
833 637 495 490 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 984 2330 2370 2616
Size: 984 1346 40 246
Fragments arranged by size:
1346 984 246 40
Tth111I GACn'n_nGTC
Cuts at: 0 2116 2616
Size: 2116 500
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1266 2616
Size: 1266 1350
XmnI GAAnn'nnTTC
Cuts at: 0 1513 2616
Size: 1513 1103
ZraI GAC'GTC
Cuts at: 0 855 2616
Size: 855 1761
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AlfI AlwNI
ApaI ApoI AvaI BaeI BanI BanII BbeI BbsI
BbvCI BcgI BclI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstYI
BtgI CspCI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI
PflMI PfoI PpuMI PspOMI PstI PvuII RsrII SacI
SacII SanDI SbfI SexAI SfcI SfoI SmlI SphI
StuI StyI TaqII Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI AvrII BamHI BciVI BfrBI BglI
BglII BmtI BsaAI BsaBI BsiWI BspEI BspHI BsrGI
BstBI BstXI BstZ17I Bsu36I BtgZI BtsI ClaI DraI
DrdI EcoRV FseI FspAI HpaI MfeI NheI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI SalI SapI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TatI TstI XbaI XhoI XmaI