Restriction map for
pF1KE0085
(Linear) (Six-base) MAPSORT of: KIEE0085.seq Check: 5403 from: 1 to: 4251
KIEE0085.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3771 4251
Size: 3771 480
AclI AA'CG_TT
Cuts at: 0 2711 4251
Size: 2711 1540
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1178 2534 2660 2716 3944 4251
Size: 460 718 1356 126 56 1228 307
Fragments arranged by size:
1356 1228 718 460 307 126 56
AflII C'TTAA_G
Cuts at: 0 426 4251
Size: 426 3825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 749 4251
Size: 749 3502
AlwNI CAG_nnn'CTG
Cuts at: 0 1822 4251
Size: 1822 2429
ApaI G_GGCC'C
Cuts at: 0 264 4251
Size: 264 3987
ApoI r'AATT_y
Cuts at: 0 199 504 772 882 1250 1329 1423 1715
Size: 199 305 268 110 368 79 94 292
Cuts at: 1715 1968 2240 2270 2555 4251
Size: 253 272 30 285 1696
Fragments arranged by size:
1696 368 305 292 285 272 268 253
199 110 94 79 30
AseI AT'TA_AT
Cuts at: 0 2222 4251
Size: 2222 2029
AvaI C'yCGr_G
Cuts at: 0 52 75 263 296 3773 4251
Size: 52 23 188 33 3477 478
Fragments arranged by size:
3477 478 188 52 33 23
BanI G'GyrC_C
Cuts at: 0 125 4251
Size: 125 4126
BanII G_rGCy'C
Cuts at: 0 63 76 264 3374 4251
Size: 63 13 188 3110 877
Fragments arranged by size:
3110 877 188 63 13
BbeI G_GCGC'C
Cuts at: 0 129 4251
Size: 129 4122
BbsI GAAGACnn'nnnn_
Cuts at: 0 1640 2680 3362 4088 4251
Size: 1640 1040 682 726 163
Fragments arranged by size:
1640 1040 726 682 163
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 221 4251
Size: 187 34 4030
Fragments arranged by size:
4030 187 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4152 4251
Size: 4152 99
Bme1580I G_kGCm'C
Cuts at: 0 264 4251
Size: 264 3987
BmrI ACTGGGnnnn_n'
Cuts at: 0 1548 3940 4251
Size: 1548 2392 311
Fragments arranged by size:
2392 1548 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2037 3232 3532 3889 4251
Size: 2037 1195 300 357 362
Fragments arranged by size:
2037 1195 362 357 300
Bpu10I CC'TnA_GC
Cuts at: 0 3431 4154 4251
Size: 3431 723 97
Fragments arranged by size:
3431 723 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 890 2125 3214 4251
Size: 847 43 1235 1089 1037
Fragments arranged by size:
1235 1089 1037 847 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 332 4251
Size: 332 3919
BsaAI yAC'GTr
Cuts at: 0 1939 3949 4251
Size: 1939 2010 302
Fragments arranged by size:
2010 1939 302
BsaBI GATnn'nnATC
Cuts at: 0 1868 4251
Size: 1868 2383
BsaHI Gr'CG_yC
Cuts at: 0 126 4251
Size: 126 4125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4232 4251
Size: 4232 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2041 2896 3485 4251
Size: 2041 855 589 766
Fragments arranged by size:
2041 855 766 589
BseYI C'CCAG_C
Cuts at: 0 334 4251
Size: 334 3917
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2885 3805 4251
Size: 2885 920 446
Fragments arranged by size:
2885 920 446
BsiHKAI G_wGCw'C
Cuts at: 0 3374 3467 4251
Size: 3374 93 784
Fragments arranged by size:
3374 784 93
BsmI GAATG_Cn'
Cuts at: 0 738 3343 3977 4251
Size: 738 2605 634 274
Fragments arranged by size:
2605 738 634 274
Bsp1286I G_dGCh'C
Cuts at: 0 63 76 264 3374 3467 4251
Size: 63 13 188 3110 93 784
Fragments arranged by size:
3110 784 188 93 63 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3771 4251
Size: 3771 480
BsrDI GCAATG_nn'
Cuts at: 0 3056 3719 4251
Size: 3056 663 532
Fragments arranged by size:
3056 663 532
BssHII G'CGCG_C
Cuts at: 0 80 308 4251
Size: 80 228 3943
Fragments arranged by size:
3943 228 80
BstAPI GCAn_nnn'nTGC
Cuts at: 0 418 1649 2158 4251
Size: 418 1231 509 2093
Fragments arranged by size:
2093 1231 509 418
BstBI TT'CG_AA
Cuts at: 0 197 4251
Size: 197 4054
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2061 3750 4251
Size: 2061 1689 501
Fragments arranged by size:
2061 1689 501
BstYI r'GATC_y
Cuts at: 0 3315 4251
Size: 3315 936
BtgI C'CryG_G
Cuts at: 0 187 4251
Size: 187 4064
BtsI GCAGTG_nn'
Cuts at: 0 162 1328 2948 4251
Size: 162 1166 1620 1303
Fragments arranged by size:
1620 1303 1166 162
DraI TTT'AAA
Cuts at: 0 1587 2352 2489 4251
Size: 1587 765 137 1762
Fragments arranged by size:
1762 1587 765 137
EarI CTCTTCn'nnn_
Cuts at: 0 389 470 1085 2636 2735 2984 4251
Size: 389 81 615 1551 99 249 1267
Fragments arranged by size:
1551 1267 615 389 249 99 81
EcoICRI GAG'CTC
Cuts at: 0 3372 4251
Size: 3372 879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1178 2037 2534 2660 2716 3232 3532
Size: 460 718 859 497 126 56 516 300
Cuts at: 3532 3889 3944 4251
Size: 357 55 307
Fragments arranged by size:
859 718 516 497 460 357 307 300
126 56 55
EcoO109I rG'GnC_Cy
Cuts at: 0 260 4251
Size: 260 3991
EcoRI G'AATT_C
Cuts at: 0 199 4251
Size: 199 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1038 3610 4246 4251
Size: 1038 2572 636 5
Fragments arranged by size:
2572 1038 636 5
HaeII r_GCGC'y
Cuts at: 0 129 4251
Size: 129 4122
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1731 1763 2190 2222 2664 2696 3066 3098
Size: 1731 32 427 32 442 32 370 32
Cuts at: 3098 3543 3575 4032 4064 4251
Size: 445 32 457 32 187
Fragments arranged by size:
1731 457 445 442 427 370 187 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1498 1986 3643 4251
Size: 1498 488 1657 608
Fragments arranged by size:
1657 1498 608 488
HindIII A'AGCT_T
Cuts at: 0 1103 1693 1723 2042 2483 4022 4251
Size: 1103 590 30 319 441 1539 229
Fragments arranged by size:
1539 1103 590 441 319 229 30
HpaI GTT'AAC
Cuts at: 0 1986 4251
Size: 1986 2265
KasI G'GCGC_C
Cuts at: 0 125 4251
Size: 125 4126
MfeI C'AATT_G
Cuts at: 0 2590 4251
Size: 2590 1661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 4251
Size: 1134 3117
MslI CAynn'nnrTG
Cuts at: 0 1321 4251
Size: 1321 2930
MspA1I CmG'CkG
Cuts at: 0 189 2016 2115 2860 2899 3325 4251
Size: 189 1827 99 745 39 426 926
Fragments arranged by size:
1827 926 745 426 189 99 39
NarI GG'CG_CC
Cuts at: 0 126 4251
Size: 126 4125
NdeI CA'TA_TG
Cuts at: 0 4031 4251
Size: 4031 220
PflMI CCAn_nnn'nTGG
Cuts at: 0 957 4251
Size: 957 3294
PfoI T'CCnGG_A
Cuts at: 0 3248 4251
Size: 3248 1003
PsiI TTA'TAA
Cuts at: 0 880 3613 4251
Size: 880 2733 638
Fragments arranged by size:
2733 880 638
PspOMI G'GGCC_C
Cuts at: 0 260 4251
Size: 260 3991
PspXI vC'TCGA_Gb
Cuts at: 0 296 4251
Size: 296 3955
PvuII CAG'CTG
Cuts at: 0 2016 2115 2860 2899 3325 4251
Size: 2016 99 745 39 426 926
Fragments arranged by size:
2016 926 745 426 99 39
SacI G_AGCT'C
Cuts at: 0 3374 4251
Size: 3374 877
SacII CC_GC'GG
Cuts at: 0 190 4251
Size: 190 4061
SapI GCTCTTCn'nnn_
Cuts at: 0 1085 2984 4251
Size: 1085 1899 1267
Fragments arranged by size:
1899 1267 1085
ScaI AGT'ACT
Cuts at: 0 3150 3191 3234 4251
Size: 3150 41 43 1017
Fragments arranged by size:
3150 1017 43 41
SfcI C'TryA_G
Cuts at: 0 2719 3268 4251
Size: 2719 549 983
Fragments arranged by size:
2719 983 549
SfoI GGC'GCC
Cuts at: 0 127 4251
Size: 127 4124
SmaI CCC'GGG
Cuts at: 0 54 265 3775 4251
Size: 54 211 3510 476
Fragments arranged by size:
3510 476 211 54
SmlI C'TyrA_G
Cuts at: 0 296 426 826 905 2104 3193 4251
Size: 296 130 400 79 1199 1089 1058
Fragments arranged by size:
1199 1089 1058 400 296 130 79
SnaBI TAC'GTA
Cuts at: 0 1939 4251
Size: 1939 2312
SpeI A'CTAG_T
Cuts at: 0 2002 4251
Size: 2002 2249
SspI AAT'ATT
Cuts at: 0 1169 2220 4251
Size: 1169 1051 2031
Fragments arranged by size:
2031 1169 1051
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 4251
Size: 337 3914
TatI w'GTAC_w
Cuts at: 0 2981 3148 3189 3232 3264 3716 3731 4251
Size: 2981 167 41 43 32 452 15 520
Fragments arranged by size:
2981 520 452 167 43 41 32 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4232 4251
Size: 4232 19
Tth111I GACn'n_nGTC
Cuts at: 0 2370 4251
Size: 2370 1881
XbaI T'CTAG_A
Cuts at: 0 1360 2208 4251
Size: 1360 848 2043
Fragments arranged by size:
2043 1360 848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 342 4251
Size: 342 3909
XhoI C'TCGA_G
Cuts at: 0 296 4251
Size: 296 3955
XmaI C'CCGG_G
Cuts at: 0 52 263 3773 4251
Size: 52 211 3510 478
Fragments arranged by size:
3510 478 211 52
XmnI GAAnn'nnTTC
Cuts at: 0 782 1148 3593 4251
Size: 782 366 2445 658
Fragments arranged by size:
2445 782 658 366
Enzymes that do cut:
AarI AclI AcuI AflII AlfI AlwNI ApaI ApoI
AseI AvaI BanI BanII BbeI BbsI BcgI BciVI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BssHII BstAPI BstBI BstXI BstYI BtgI
BtsI DraI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI
MslI MspA1I NarI NdeI PflMI PfoI PsiI PspOMI
PspXI PvuII SacI SacII SapI ScaI SfcI SfoI
SmaI SmlI SnaBI SpeI SspI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflIII AgeI AhdI AleI
AloI ApaLI AscI AsiSI AvrII BaeI BamHI BbvCI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssSI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoNI EcoRV FseI
FspI FspAI KpnI MluI MscI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PpuMI PshAI PsrI PstI PvuI
RsrII SalI SanDI SbfI SexAI SfiI SgfI SgrAI
SphI SrfI StuI StyI SwaI ZraI