Restriction map for
pF1KE0086
(Linear) (Six-base) MAPSORT of: KIEE0086.seq Check: 9182 from: 1 to: 1575
KIEE0086.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 888 1125 1575
Size: 888 237 450
Fragments arranged by size:
888 450 237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1302 1575
Size: 658 644 273
Fragments arranged by size:
658 644 273
AfeI AGC'GCT
Cuts at: 0 316 611 1575
Size: 316 295 964
Fragments arranged by size:
964 316 295
AleI CACnn'nnGTG
Cuts at: 0 796 1534 1575
Size: 796 738 41
Fragments arranged by size:
796 738 41
AlwNI CAG_nnn'CTG
Cuts at: 0 894 1575
Size: 894 681
ApaI G_GGCC'C
Cuts at: 0 83 193 247 1244 1420 1575
Size: 83 110 54 997 176 155
Fragments arranged by size:
997 176 155 110 83 54
ApaLI G'TGCA_C
Cuts at: 0 355 1575
Size: 355 1220
AvaI C'yCGr_G
Cuts at: 0 57 76 193 246 613 1555 1575
Size: 57 19 117 53 367 942 20
Fragments arranged by size:
942 367 117 57 53 20 19
BamHI G'GATC_C
Cuts at: 0 1228 1575
Size: 1228 347
BanI G'GyrC_C
Cuts at: 0 603 1435 1480 1575
Size: 603 832 45 95
Fragments arranged by size:
832 603 95 45
BanII G_rGCy'C
Cuts at: 0 83 193 247 380 1244 1420 1575
Size: 83 110 54 133 864 176 155
Fragments arranged by size:
864 176 155 133 110 83 54
BbeI G_GCGC'C
Cuts at: 0 1484 1575
Size: 1484 91
BglI GCCn_nnn'nGGC
Cuts at: 0 106 1575
Size: 106 1469
BlpI GC'TnA_GC
Cuts at: 0 379 1332 1575
Size: 379 953 243
Fragments arranged by size:
953 379 243
Bme1580I G_kGCm'C
Cuts at: 0 37 64 83 193 247 359 1244 1420
Size: 37 27 19 110 54 112 885 176
Cuts at: 1420 1575
Size: 155
Fragments arranged by size:
885 176 155 112 110 54 37 27
19
BmgBI CAC'GTC
Cuts at: 0 354 495 1575
Size: 354 141 1080
Fragments arranged by size:
1080 354 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 832 1575
Size: 832 743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 829 1575
Size: 745 84 746
Fragments arranged by size:
746 745 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1396 1575
Size: 508 888 179
Fragments arranged by size:
888 508 179
BsaHI Gr'CG_yC
Cuts at: 0 172 752 904 1481 1575
Size: 172 580 152 577 94
Fragments arranged by size:
580 577 172 152 94
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 988 1575
Size: 247 741 587
Fragments arranged by size:
741 587 247
BseYI C'CCAG_C
Cuts at: 0 82 1148 1575
Size: 82 1066 427
Fragments arranged by size:
1066 427 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 568 1024 1134 1575
Size: 328 240 456 110 441
Fragments arranged by size:
456 441 328 240 110
BsiEI CG_ry'CG
Cuts at: 0 125 1514 1575
Size: 125 1389 61
Fragments arranged by size:
1389 125 61
BsiHKAI G_wGCw'C
Cuts at: 0 359 1575
Size: 359 1216
BsmBI CGTCTCn'nnnn_
Cuts at: 0 556 1575
Size: 556 1019
Bsp1286I G_dGCh'C
Cuts at: 0 37 64 83 193 247 359 380 1244
Size: 37 27 19 110 54 112 21 864
Cuts at: 1244 1420 1575
Size: 176 155
Fragments arranged by size:
864 176 155 112 110 54 37 27
21 19
BspHI T'CATG_A
Cuts at: 0 962 1575
Size: 962 613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 656 888 976 1125 1498 1575
Size: 656 232 88 149 373 77
Fragments arranged by size:
656 373 232 149 88 77
BsrBI CCG'CTC
Cuts at: 0 17 225 960 1575
Size: 17 208 735 615
Fragments arranged by size:
735 615 208 17
BsrDI GCAATG_nn'
Cuts at: 0 621 1575
Size: 621 954
BsrFI r'CCGG_y
Cuts at: 0 1082 1365 1575
Size: 1082 283 210
Fragments arranged by size:
1082 283 210
BssSI C'ACGA_G
Cuts at: 0 1064 1462 1575
Size: 1064 398 113
Fragments arranged by size:
1064 398 113
BstAPI GCAn_nnn'nTGC
Cuts at: 0 894 1575
Size: 894 681
BstYI r'GATC_y
Cuts at: 0 1228 1550 1575
Size: 1228 322 25
Fragments arranged by size:
1228 322 25
BtgI C'CryG_G
Cuts at: 0 212 419 473 761 1236 1475 1535 1575
Size: 212 207 54 288 475 239 60 40
Fragments arranged by size:
475 288 239 212 207 60 54 40
BtsI GCAGTG_nn'
Cuts at: 0 517 743 1575
Size: 517 226 832
Fragments arranged by size:
832 517 226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 462 497 1575
Size: 462 35 1078
Fragments arranged by size:
1078 462 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1464 1575
Size: 1464 111
EaeI y'GGCC_r
Cuts at: 0 122 416 461 710 1511 1545 1575
Size: 122 294 45 249 801 34 30
Fragments arranged by size:
801 294 249 122 45 34 30
EagI C'GGCC_G
Cuts at: 0 122 1511 1575
Size: 122 1389 64
Fragments arranged by size:
1389 122 64
EarI CTCTTCn'nnn_
Cuts at: 0 334 697 1039 1575
Size: 334 363 342 536
Fragments arranged by size:
536 363 342 334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 933 1575
Size: 244 689 642
Fragments arranged by size:
689 642 244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 745 829 1302 1575
Size: 658 87 84 473 273
Fragments arranged by size:
658 473 273 87 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1450 1575
Size: 1450 125
EcoO109I rG'GnC_Cy
Cuts at: 0 190 936 988 1416 1575
Size: 190 746 52 428 159
Fragments arranged by size:
746 428 190 159 52
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1218 1509 1575
Size: 1218 291 66
Fragments arranged by size:
1218 291 66
FspI TGC'GCA
Cuts at: 0 1450 1575
Size: 1450 125
HaeII r_GCGC'y
Cuts at: 0 89 262 318 431 613 1484 1575
Size: 89 173 56 113 182 871 91
Fragments arranged by size:
871 182 173 113 91 89 56
HincII GTy'rAC
Cuts at: 0 1053 1191 1389 1575
Size: 1053 138 198 186
Fragments arranged by size:
1053 198 186 138
KasI G'GCGC_C
Cuts at: 0 1480 1575
Size: 1480 95
MscI TGG'CCA
Cuts at: 0 463 712 1575
Size: 463 249 863
Fragments arranged by size:
863 463 249
MslI CAynn'nnrTG
Cuts at: 0 796 1009 1204 1534 1575
Size: 796 213 195 330 41
Fragments arranged by size:
796 330 213 195 41
MspA1I CmG'CkG
Cuts at: 0 214 505 708 763 863 885 894 1091
Size: 214 291 203 55 100 22 9 197
Cuts at: 1091 1160 1322 1575
Size: 69 162 253
Fragments arranged by size:
291 253 214 203 197 162 100 69
55 22 9
NarI GG'CG_CC
Cuts at: 0 1481 1575
Size: 1481 94
NotI GC'GGCC_GC
Cuts at: 0 122 1575
Size: 122 1453
PasI CC'CwG_GG
Cuts at: 0 163 939 1575
Size: 163 776 636
Fragments arranged by size:
776 636 163
PflMI CCAn_nnn'nTGG
Cuts at: 0 627 1137 1575
Size: 627 510 438
Fragments arranged by size:
627 510 438
PfoI T'CCnGG_A
Cuts at: 0 806 1575
Size: 806 769
PpuMI rG'GwC_Cy
Cuts at: 0 988 1575
Size: 988 587
PspOMI G'GGCC_C
Cuts at: 0 79 189 243 1240 1416 1575
Size: 79 110 54 997 176 159
Fragments arranged by size:
997 176 159 110 79 54
PspXI vC'TCGA_Gb
Cuts at: 0 613 1575
Size: 613 962
PstI C_TGCA'G
Cuts at: 0 266 750 899 987 1575
Size: 266 484 149 88 588
Fragments arranged by size:
588 484 266 149 88
PvuII CAG'CTG
Cuts at: 0 708 894 1322 1575
Size: 708 186 428 253
Fragments arranged by size:
708 428 253 186
SacII CC_GC'GG
Cuts at: 0 215 764 1575
Size: 215 549 811
Fragments arranged by size:
811 549 215
SfcI C'TryA_G
Cuts at: 0 262 746 895 983 1155 1575
Size: 262 484 149 88 172 420
Fragments arranged by size:
484 420 262 172 149 88
SfoI GGC'GCC
Cuts at: 0 1482 1575
Size: 1482 93
SmaI CCC'GGG
Cuts at: 0 59 78 195 248 1575
Size: 59 19 117 53 1327
Fragments arranged by size:
1327 117 59 53 19
SmlI C'TyrA_G
Cuts at: 0 523 613 1411 1575
Size: 523 90 798 164
Fragments arranged by size:
798 523 164 90
SrfI GCCC'GGGC
Cuts at: 0 59 1575
Size: 59 1516
StuI AGG'CCT
Cuts at: 0 1445 1575
Size: 1445 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 812 844 1575
Size: 812 32 731
Fragments arranged by size:
812 731 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 720 1065 1575
Size: 720 345 510
Fragments arranged by size:
720 510 345
XhoI C'TCGA_G
Cuts at: 0 613 1575
Size: 613 962
XmaI C'CCGG_G
Cuts at: 0 57 76 193 246 1575
Size: 57 19 117 53 1329
Fragments arranged by size:
1329 117 57 53 19
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BglI BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstYI BtgI BtsI CspCI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI
HaeII HincII KasI MscI MslI MspA1I NarI NotI
PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
SacII SfcI SfoI SmaI SmlI SrfI StuI TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BsiWI
BsmI BspEI BsrGI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII EcoICRI EcoRI EcoRV
FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI StyI SwaI TaqII TatI Tth111I XbaI XmnI
ZraI